Prupe.6G130400_v2.0.a1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0011144
Physical Location & Seq
Reverse (-)
10155580 .. 10159083
3504 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G130400.2

Sequence Viewer

Length: 729 bp
ATGGCTTCCAATGGGAAACATGGGTCTCCCTCTTATGTGCCCTCTGCCCCATCCCTGCCCGAGTCCTACAGGCAGCAGTACTTTCAGGGGAGTGGCGGCTCCTATGGTTACTGGCAGCAGCACTCTCAGGGCAATGGCAGCTCCTATAGTTACGGGCAGCAGCAGGGGCCTTCTTCATACGGGCACTCTGGTTTTCCTCCGGGAACAGACCCAGCTGTGATTAGCAGCTTTCAGATGGTCGACAGGGACAGGAGTGGTTTTATTGATGATAATGAGCTGCAGCAAGCTCTTTCTTCTGGGTACCAGAGATTTAGCCTCAGGACCATTCGCTTGCTCATCTTTCTCTTCAAAAGCCCCAATGATCCTCTCCGAGTTGGCCCCAAAGAGTTTGCTGCACTCTGGACTTGTCTTGGTCAATGGCGAGGCATATTTGAGAAATATGACAAAGATAGGAGTGGGAAAATTGATTCGATGGAACTGAGGGATGCACTCTACAGTCTAGGCCTTGCCATCCCACCTTCTGTTCTTCAACTTTTGATTTCCAAGTATGATGATGGAAGTGGGAGCAGGGTTGAACTCAATTTCGATAGTTTTGTGGAATGCGGGATGATTGTGAAGGGTTTGACTGATAAGTTCAAAGAGAGAGATCTGCGATATACCGGTTCGGCAACAATTAATTATGATACATTCATGTCTATGGTCATCCCATTTTTTGTGTCATATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

243

Amino Acids

27.05

Weight (kDa)

6.3

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 300
AccB1I GGYRCC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 240
AciI CCGC 2 cut(s) 96, 603
AclWI GGATC 1 cut(s) 356
AfaI GTAC 2 cut(s) 80, 302
AgeI ACCGGT 1 cut(s) 659
AgsI TTSAA 4 cut(s) 349, 530, 575, 637
AluBI AGCT 5 cut(s) 141, 215, 228, 277, 287
AluI AGCT 5 cut(s) 141, 215, 228, 277, 287
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 1 cut(s) 356
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 3 cut(s) 167, 376, 502
AseI ATTAAT 1 cut(s) 675
AsiGI ACCGGT 1 cut(s) 659
Asp718I GGTACC 1 cut(s) 300
AspS9I GGNCC 3 cut(s) 167, 321, 377
AsuC2I CCSGG 1 cut(s) 201
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 317
BaeGI GKGCMC 2 cut(s) 42, 186
BanI GGYRCC 1 cut(s) 300
BccI CCATC 5 cut(s) 58, 229, 466, 518, 548
BcnI CCSGG 1 cut(s) 201
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 1 cut(s) 500
BfmI CTRYAG 4 cut(s) 67, 145, 278, 493
BglII AGATCT 1 cut(s) 646
BmcAI AGTACT 1 cut(s) 80
Bme1390I CCNGG 1 cut(s) 201
Bme18I GGWCC 1 cut(s) 321
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 3 cut(s) 167, 321, 377
BmiI GGNNCC 4 cut(s) 100, 168, 302, 379
BmrFI CCNGG 1 cut(s) 201
BmsI GCATC 1 cut(s) 475
BpuMI CCSGG 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 659
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 1 cut(s) 116
Bse21I CCTNAGG 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 139
BseGI GGATG 5 cut(s) 50, 490, 510, 612, 702
BseMI GCAATG 1 cut(s) 139
BseMII CTCAG 3 cut(s) 140, 331, 470
BseNI ACTGG 1 cut(s) 116
BseSI GKGCMC 2 cut(s) 42, 186
BseYI CCCAGC 1 cut(s) 211
BsgI GTGCAG 1 cut(s) 378
BshFI GGCC 3 cut(s) 169, 378, 504
BshNI GGYRCC 1 cut(s) 300
BshTI ACCGGT 1 cut(s) 659
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 2 cut(s) 200, 660
BslFI GGGAC 1 cut(s) 260
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 260
BsmI GAATGC 1 cut(s) 605
BsnI GGCC 3 cut(s) 169, 378, 504
Bso31I GGTCTC 1 cut(s) 30
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 2 cut(s) 42, 186
Bsp143I GATC 2 cut(s) 361, 646
BspACI CCGC 2 cut(s) 96, 603
BspANI GGCC 3 cut(s) 169, 378, 504
BspCNI CTCAG 3 cut(s) 139, 330, 471
BspLI GGNNCC 4 cut(s) 100, 168, 302, 379
BspMAI CTGCAG 1 cut(s) 282
BspPI GGATC 1 cut(s) 356
BspT107I GGYRCC 1 cut(s) 300
BspTNI GGTCTC 1 cut(s) 30
BsrDI GCAATG 1 cut(s) 139
BsrFI RCCGGY 1 cut(s) 659
BsrI ACTGG 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 659
BssMI GATC 2 cut(s) 361, 646
Bst4CI ACNGT 1 cut(s) 497
Bst6I CTCTTC 1 cut(s) 350
BstC8I GCNNGC 2 cut(s) 285, 332
BstDEI CTNAG 3 cut(s) 126, 317, 479
BstF5I GGATG 5 cut(s) 50, 490, 510, 612, 702
BstKTI GATC 2 cut(s) 364, 649
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 2 cut(s) 361, 646
BstMWI GCNNNNNNNGC 2 cut(s) 138, 166
BstSCI CCNGG 1 cut(s) 199
BstSFI CTRYAG 4 cut(s) 67, 145, 278, 493
BstSLI GKGCMC 2 cut(s) 42, 186
BstX2I RGATCY 1 cut(s) 646
BstYI RGATCY 1 cut(s) 646
Bsu36I CCTNAGG 1 cut(s) 317
BsuRI GGCC 3 cut(s) 169, 378, 504
BtsCI GGATG 5 cut(s) 50, 490, 510, 612, 702
Cac8I GCNNGC 2 cut(s) 285, 332
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 3 cut(s) 167, 321, 377
Csp6I GTAC 2 cut(s) 79, 301
CspAI ACCGGT 1 cut(s) 659
CviAII CATG 2 cut(s) 20, 691
CviQI GTAC 2 cut(s) 79, 301
DdeI CTNAG 3 cut(s) 126, 317, 479
DpnI GATC 2 cut(s) 363, 648
DpnII GATC 2 cut(s) 361, 646
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Eco147I AGGCCT 1 cut(s) 504
Eco31I GGTCTC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 321
Eco81I CCTNAGG 1 cut(s) 317
Eco88I CYCGRG 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 167
FaeI CATG 2 cut(s) 23, 694
FaqI GGGAC 1 cut(s) 260
FatI CATG 2 cut(s) 19, 690
FauI CCCGC 1 cut(s) 596
FblI GTMKAC 1 cut(s) 240
FokI GGATG 5 cut(s) 37, 497, 497, 619, 689
FspBI CTAG 1 cut(s) 500
GsaI CCCAGC 1 cut(s) 215
HaeIII GGCC 3 cut(s) 169, 378, 504
HapII CCGG 2 cut(s) 200, 660
Hin1II CATG 2 cut(s) 23, 694
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 2 cut(s) 62, 467
HpaII CCGG 2 cut(s) 200, 660
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 2 cut(s) 234, 371
Hpy188III TCNNGA 2 cut(s) 319, 400
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 3 cut(s) 180, 528, 610
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4V TGCA 3 cut(s) 280, 395, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 166
HpyF3I CTNAG 3 cut(s) 126, 317, 479
Hsp92II CATG 2 cut(s) 23, 694
KpnI GGTACC 1 cut(s) 304
Kzo9I GATC 2 cut(s) 361, 646
LmnI GCTCC 3 cut(s) 104, 146, 564
LweI GCATC 1 cut(s) 475
MaeI CTAG 1 cut(s) 500
MaeIII GTNAC 2 cut(s) 107, 149
MalI GATC 2 cut(s) 363, 648
MboI GATC 2 cut(s) 361, 646
MboII GAAGA 4 cut(s) 165, 285, 337, 518
MflI RGATCY 1 cut(s) 646
MhlI GDGCHC 2 cut(s) 42, 186
MluCI AATT 5 cut(s) 462, 580, 672, 676, 724
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 7 cut(s) 40, 52, 207, 326, 375, 416, 474
MseI TTAA 1 cut(s) 675
MslI CAYNNNNRTG 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 215
MspI CCGG 2 cut(s) 200, 660
MspR9I CCNGG 1 cut(s) 201
Mva1269I GAATGC 1 cut(s) 605
MwoI GCNNNNNNNGC 2 cut(s) 138, 166
NciI CCSGG 1 cut(s) 201
NdeII GATC 2 cut(s) 361, 646
NlaIII CATG 2 cut(s) 23, 694
NlaIV GGNNCC 4 cut(s) 100, 168, 302, 379
PceI AGGCCT 1 cut(s) 504
PctI GAATGC 1 cut(s) 605
PfeI GAWTC 1 cut(s) 467
PfoI TCCNGGA 1 cut(s) 199
PinAI ACCGGT 1 cut(s) 659
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshBI ATTAAT 1 cut(s) 675
PspFI CCCAGC 1 cut(s) 211
PspN4I GGNNCC 4 cut(s) 100, 168, 302, 379
PspPI GGNCC 3 cut(s) 167, 321, 377
PstI CTGCAG 1 cut(s) 282
PsuI RGATCY 1 cut(s) 646
PvuII CAGCTG 1 cut(s) 215
RsaI GTAC 2 cut(s) 80, 302
RsaNI GTAC 2 cut(s) 79, 301
RseI CAYNNNNRTG 1 cut(s) 695
SalI GTCGAC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 675
Sau3AI GATC 2 cut(s) 361, 646
Sau96I GGNCC 3 cut(s) 167, 321, 377
ScaI AGTACT 1 cut(s) 80
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 201
SduI GDGCHC 2 cut(s) 42, 186
SetI ASST 6 cut(s) 143, 217, 230, 279, 289, 520
SfaNI GCATC 1 cut(s) 475
SfcI CTRYAG 4 cut(s) 67, 145, 278, 493
SinI GGWCC 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 695
Sse9I AATT 5 cut(s) 462, 580, 672, 676, 724
SseBI AGGCCT 1 cut(s) 504
SsiI CCGC 2 cut(s) 96, 603
SspMI CTAG 1 cut(s) 500
StuI AGGCCT 1 cut(s) 504
StyD4I CCNGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 497
TaqI TCGA 3 cut(s) 240, 470, 585
TasI AATT 5 cut(s) 462, 580, 672, 676, 724
TatI WGTACW 1 cut(s) 78
TauI GCSGC 1 cut(s) 99
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 1 cut(s) 675
Tru9I TTAA 1 cut(s) 675
TspDTI ATGAA 2 cut(s) 165, 679
VpaK11BI GGWCC 1 cut(s) 321
VspI ATTAAT 1 cut(s) 675
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 1 cut(s) 500
ZrmI AGTACT 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.