MD03G1144700.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011144
Physical Location & Seq
Reverse (-)
15607935 .. 15609834
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1144700.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGCTTCCAATGGGAAATACGGCTCTCACCCTTACGCGCCCTCTGCGCCATCCCTGCCCGATTCCTACGGTCCCCAGCACTCCCAGTACTCCCATGGTTACGGGCAGCCACAGCCGCAGCCGCAGCCACAGGGGTGGTCTTCTTCTTTTTCTTCATATGGGCAGTCGGGTTTTCCAGCGGGGACAGACCCGGCTGTGATTAGAAGCTTTGAGATGGTGGATAGGGACAGGAGTGGATACATCGACGAGAACGAGCTGCAGCAAGCTCTGTCTTCTGGGTACCAGAGGTTTAGCCTCAGGACCATTCGCCTGCTCATTTTTCTCTTCAAAAGTCCCACGGATTCTCTCAGAGTTGGGCCCAAGGAATTTGCTGCGCTCTGGATTTGTCTTGGACAATGGCGATCCATATTCGAGAGGTATGACAAAGATGGGAGTGGAAAGATTGATTCGATGGAACTCAGAGATGCACTCTACTACGGTCTAGGCCTTGCCATGCCACCTTCTGTTCTTCAACTTTTGATTTCCAAGTATGATGATGGAAGTGGCCGCAGGGTTGAACTAAACTTTGACAGTTTCGTGGAATGCGGGATGATTGTGAGGGGTTTGACCGATAGGTTCAAAGAGAAAGATCGGCGGTATACTGGTTCAGCAAATTTTGGTTACGACGAATTTATGTCTATGGTCATCCCATTTCTTGTGTCGTACAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

237

Amino Acids

26.57

Weight (kDa)

5.94

Isoelectric Point (pI)

53.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 132 - 158 5.8e-07 EF-hand domain
EF-hand_1 PF00036 133 - 157 4.7e-06 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 279
AccI GTMKAC 1 cut(s) 638
AccII CGCG 1 cut(s) 38
AciI CCGC 6 cut(s) 116, 122, 179, 547, 585, 634
AclWI GGATC 1 cut(s) 396
AcoI YGGCCR 1 cut(s) 544
AcsI RAATTY 3 cut(s) 365, 652, 668
AfaI GTAC 3 cut(s) 89, 281, 704
AgsI TTSAA 4 cut(s) 328, 512, 557, 619
AleI CACNNNNGTG 1 cut(s) 133
AluBI AGCT 3 cut(s) 207, 256, 266
AluI AGCT 3 cut(s) 207, 256, 266
AlwI GGATC 1 cut(s) 396
AoxI GGCC 3 cut(s) 356, 484, 544
ApaI GGGCCC 1 cut(s) 360
ApeKI GCWGC 6 cut(s) 106, 118, 124, 256, 259, 371
ApoI RAATTY 3 cut(s) 365, 652, 668
Asp718I GGTACC 1 cut(s) 279
AspLEI GCGC 3 cut(s) 40, 49, 376
AspS9I GGNCC 4 cut(s) 71, 300, 356, 357
AsuC2I CCSGG 1 cut(s) 191
AsuHPI GGTGA 1 cut(s) 20
AvaII GGWCC 2 cut(s) 71, 300
AxyI CCTNAGG 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 360
BanI GGYRCC 1 cut(s) 279
BanII GRGCYC 1 cut(s) 360
BbsI GAAGAC 2 cut(s) 132, 264
BbvI GCAGC 6 cut(s) 118, 130, 136, 243, 271, 358
BccI CCATC 5 cut(s) 58, 208, 422, 445, 530
BceAI ACGGC 1 cut(s) 37
BciVI GTATCC 1 cut(s) 230
BcnI CCSGG 1 cut(s) 191
BfaI CTAG 1 cut(s) 482
BfmI CTRYAG 1 cut(s) 257
BfuI GTATCC 1 cut(s) 230
BisI GCNGC 9 cut(s) 107, 116, 119, 122, 125, 257, 260, 372, 547
BlsI GCNGC 9 cut(s) 108, 117, 120, 123, 126, 258, 261, 373, 548
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 2 cut(s) 71, 300
BmgT120I GGNCC 4 cut(s) 71, 300, 356, 357
BmiI GGNNCC 3 cut(s) 73, 281, 358
BmrFI CCNGG 1 cut(s) 191
BmrI ACTGGG 1 cut(s) 79
BmsI GCATC 1 cut(s) 454
BmuI ACTGGG 1 cut(s) 79
BpiI GAAGAC 2 cut(s) 132, 264
BplI GAGNNNNNCTC 2 cut(s) 453, 485
BpuMI CCSGG 1 cut(s) 191
BsaJI CCNNGG 3 cut(s) 94, 336, 360
Bse1I ACTGG 2 cut(s) 85, 646
Bse21I CCTNAGG 1 cut(s) 296
BseDI CCNNGG 3 cut(s) 94, 336, 360
BseGI GGATG 3 cut(s) 50, 594, 684
BseMII CTCAG 3 cut(s) 310, 361, 472
BseNI ACTGG 2 cut(s) 85, 646
BseSI GKGCMC 1 cut(s) 360
BseXI GCAGC 6 cut(s) 118, 130, 136, 243, 271, 358
BseYI CCCAGC 1 cut(s) 75
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 3 cut(s) 358, 486, 546
BshNI GGYRCC 1 cut(s) 279
BsiSI CCGG 1 cut(s) 191
BslFI GGGAC 4 cut(s) 57, 196, 239, 318
BsmFI GGGAC 4 cut(s) 57, 196, 239, 318
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 3 cut(s) 358, 486, 546
Bsp120I GGGCCC 1 cut(s) 356
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 2 cut(s) 401, 628
Bsp19I CCATGG 1 cut(s) 94
BspACI CCGC 6 cut(s) 116, 122, 179, 547, 585, 634
BspANI GGCC 3 cut(s) 358, 486, 546
BspCNI CTCAG 3 cut(s) 309, 360, 471
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 3 cut(s) 73, 281, 358
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 1 cut(s) 396
BspT107I GGYRCC 1 cut(s) 279
BsrI ACTGG 2 cut(s) 85, 646
BssECI CCNNGG 3 cut(s) 94, 336, 360
BssMI GATC 2 cut(s) 401, 628
BssNAI GTATAC 1 cut(s) 639
BssT1I CCWWGG 2 cut(s) 94, 360
Bst1107I GTATAC 1 cut(s) 639
Bst4CI ACNGT 3 cut(s) 71, 479, 572
Bst6I CTCTTC 1 cut(s) 329
BstC8I GCNNGC 2 cut(s) 264, 311
BstDEI CTNAG 3 cut(s) 296, 347, 458
BstDSI CCRYGG 2 cut(s) 94, 336
BstF5I GGATG 3 cut(s) 50, 594, 684
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 3 cut(s) 40, 49, 376
BstKTI GATC 2 cut(s) 404, 631
BstMBI GATC 2 cut(s) 401, 628
BstMWI GCNNNNNNNGC 7 cut(s) 44, 46, 55, 112, 115, 121, 124
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 360
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 6 cut(s) 118, 130, 136, 243, 271, 358
BstV2I GAAGAC 2 cut(s) 132, 264
BstXI CCANNNNNNTGG 1 cut(s) 135
BstZ17I GTATAC 1 cut(s) 639
Bsu36I CCTNAGG 1 cut(s) 296
BsuI GTATCC 1 cut(s) 230
BsuRI GGCC 3 cut(s) 358, 486, 546
BtgI CCRYGG 2 cut(s) 94, 336
BtsCI GGATG 3 cut(s) 50, 594, 684
Cac8I GCNNGC 2 cut(s) 264, 311
CfoI GCGC 3 cut(s) 40, 49, 376
Cfr13I GGNCC 4 cut(s) 71, 300, 356, 357
Csp6I GTAC 3 cut(s) 88, 280, 703
CviAII CATG 2 cut(s) 95, 493
CviQI GTAC 3 cut(s) 88, 280, 703
DdeI CTNAG 3 cut(s) 296, 347, 458
DpnI GATC 2 cut(s) 403, 630
DpnII GATC 2 cut(s) 401, 628
EaeI YGGCCR 1 cut(s) 544
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 2 cut(s) 94, 360
Eco147I AGGCCT 1 cut(s) 486
Eco24I GRGCYC 1 cut(s) 360
Eco47I GGWCC 2 cut(s) 71, 300
Eco81I CCTNAGG 1 cut(s) 296
EcoT14I CCWWGG 2 cut(s) 94, 360
EcoT38I GRGCYC 1 cut(s) 360
ErhI CCWWGG 2 cut(s) 94, 360
FaeI CATG 2 cut(s) 98, 496
FaqI GGGAC 4 cut(s) 57, 196, 239, 318
FatI CATG 2 cut(s) 94, 492
FauI CCCGC 2 cut(s) 172, 578
FauNDI CATATG 1 cut(s) 157
FblI GTMKAC 1 cut(s) 638
Fnu4HI GCNGC 9 cut(s) 107, 116, 119, 122, 125, 257, 260, 372, 547
FokI GGATG 3 cut(s) 37, 601, 671
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 9 cut(s) 107, 116, 119, 122, 125, 257, 260, 372, 547
FspBI CTAG 1 cut(s) 482
GlaI GCGC 3 cut(s) 39, 48, 375
GluI GCNGC 9 cut(s) 107, 116, 119, 122, 125, 257, 260, 372, 547
GsaI CCCAGC 1 cut(s) 79
HaeIII GGCC 3 cut(s) 358, 486, 546
HapII CCGG 1 cut(s) 191
HhaI GCGC 3 cut(s) 40, 49, 376
Hin1II CATG 2 cut(s) 98, 496
Hin6I GCGC 3 cut(s) 38, 47, 374
HinP1I GCGC 3 cut(s) 38, 47, 374
HindIII AAGCTT 1 cut(s) 205
HinfI GANTC 3 cut(s) 62, 341, 446
HpaII CCGG 1 cut(s) 191
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 1 cut(s) 639
Hpy188I TCNGA 2 cut(s) 350, 461
Hpy188III TCNNGA 3 cut(s) 298, 379, 412
Hpy8I GTNNAC 1 cut(s) 639
Hpy99I CGWCG 2 cut(s) 248, 668
HpyAV CCTTC 1 cut(s) 510
HpyCH4III ACNGT 3 cut(s) 71, 479, 572
HpyCH4V TGCA 2 cut(s) 259, 467
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 46, 55, 112, 115, 121, 124
HpyF3I CTNAG 3 cut(s) 296, 347, 458
Hsp92II CATG 2 cut(s) 98, 496
HspAI GCGC 3 cut(s) 38, 47, 374
KpnI GGTACC 1 cut(s) 283
Kzo9I GATC 2 cut(s) 401, 628
Lsp1109I GCAGC 6 cut(s) 118, 130, 136, 243, 271, 358
LweI GCATC 1 cut(s) 454
MaeI CTAG 1 cut(s) 482
MaeIII GTNAC 2 cut(s) 98, 659
MalI GATC 2 cut(s) 403, 630
MboI GATC 2 cut(s) 401, 628
MboII GAAGA 6 cut(s) 132, 135, 144, 264, 316, 500
MfeI CAATTG 1 cut(s) 706
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 4 cut(s) 365, 652, 668, 706
MnlI CCTC 5 cut(s) 52, 279, 305, 408, 591
MslI CAYNNNNRTG 1 cut(s) 133
MspA1I CMGCKG 1 cut(s) 179
MspI CCGG 1 cut(s) 191
MspR9I CCNGG 1 cut(s) 191
MunI CAATTG 1 cut(s) 706
Mva1269I GAATGC 1 cut(s) 587
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 7 cut(s) 44, 46, 55, 112, 115, 121, 124
NciI CCSGG 1 cut(s) 191
NcoI CCATGG 1 cut(s) 94
NdeI CATATG 1 cut(s) 157
NdeII GATC 2 cut(s) 401, 628
NlaIII CATG 2 cut(s) 98, 496
NlaIV GGNNCC 3 cut(s) 73, 281, 358
OliI CACNNNNGTG 1 cut(s) 133
PceI AGGCCT 1 cut(s) 486
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PctI GAATGC 1 cut(s) 587
PfeI GAWTC 3 cut(s) 62, 341, 446
PkrI GCNGC 9 cut(s) 108, 117, 120, 123, 126, 258, 261, 373, 548
PspFI CCCAGC 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 73, 281, 358
PspOMI GGGCCC 1 cut(s) 356
PspPI GGNCC 4 cut(s) 71, 300, 356, 357
PstI CTGCAG 1 cut(s) 261
RsaI GTAC 3 cut(s) 89, 281, 704
RsaNI GTAC 3 cut(s) 88, 280, 703
RseI CAYNNNNRTG 1 cut(s) 133
SatI GCNGC 9 cut(s) 107, 116, 119, 122, 125, 257, 260, 372, 547
Sau3AI GATC 2 cut(s) 401, 628
Sau96I GGNCC 4 cut(s) 71, 300, 356, 357
ScaI AGTACT 1 cut(s) 89
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 360
SetI ASST 7 cut(s) 209, 258, 268, 290, 419, 502, 617
SfaNI GCATC 1 cut(s) 454
SfcI CTRYAG 1 cut(s) 257
SinI GGWCC 2 cut(s) 71, 300
SmiMI CAYNNNNRTG 1 cut(s) 133
Sse9I AATT 4 cut(s) 365, 652, 668, 706
SseBI AGGCCT 1 cut(s) 486
SsiI CCGC 6 cut(s) 116, 122, 179, 547, 585, 634
SspMI CTAG 1 cut(s) 482
StuI AGGCCT 1 cut(s) 486
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 2 cut(s) 94, 360
TaaI ACNGT 3 cut(s) 71, 479, 572
TaqI TCGA 3 cut(s) 243, 411, 449
TaqII GACCGA 1 cut(s) 623
TasI AATT 4 cut(s) 365, 652, 668, 706
TatI WGTACW 1 cut(s) 87
TauI GCSGC 3 cut(s) 118, 124, 549
TfiI GAWTC 3 cut(s) 62, 341, 446
TseI GCWGC 6 cut(s) 106, 118, 124, 256, 259, 371
TspDTI ATGAA 1 cut(s) 144
TspGWI ACGGA 1 cut(s) 353
VpaK11BI GGWCC 2 cut(s) 71, 300
XapI RAATTY 3 cut(s) 365, 652, 668
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 638
XspI CTAG 1 cut(s) 482
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.