Rh5BG307200

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0011144
Physical Location & Seq
Reverse (-)
40061091 .. 40078916
17826 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG307200.1

Sequence Viewer

Length: 792 bp
ATGGCTTTTTCTAATGCCGACTATGGTTCTAACTCATACGCTGCTTCTGCCCCATCCCTGCCCGACTCCCCTGAGCACCAAGAACAAGAATATTACAGCACCACCTTTTCAAATGATCCTCCTCCTCCTGCGGCCAGAAATTACTACTACGGGAACAGCAAGCCTTTCTCACAATACGGGCGTAGTTACGCCCGTAGTTACGGCCATAGTTACGCCCGTAGATACGAGGGCCCGTCTCCTTCTTCATATGGGGAATCGGGAGGGGATTTTCCACCGGGAACCGACCCGGCTGTGATTAGCAGCTTTCAGATGGTGGATAAGGACAGGAGTGGCTTCATTGATGACAATGAATTGCAACTAGCTCTTTCTTCTGGCTACCATAAGTTTAGCCTCAGGACCATTCGCTTGCTAATGTTTCTCTTCAAAAATCCCGACAATGATTATATTCTAAAACTCGGTCCTCAGGAATTTGCTGCACTCTGGAGTTGTCTTGGTGAATGGCGAACCATGTTTCAAAAATATGACACAGATAGGAGTGGGAAGATTGATGTGCTGGAGTTGAGAGATGCACTGTATGGTCTAGGGCTTGCAGTCCCTCCTACTGTTCTTCAAGTTTTGACTTCCAGGTACGAAGATGGAAGTGGGCGTCGGGCTGAACTCGATTTTGACAGTTTTGTCGAATGTGGGATGATTGTAAAGGGCTTGACTGAGAAGTTCAAAGAGAAGGATCCGCGATATACTGGTTCAGCAACGTTCAGTTACAACGCATTTATGTCTATGAATGTTAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

263

Amino Acids

29.53

Weight (kDa)

5.08

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 166 - 192 1.7e-06 EF hand domain
EF-hand_6 PF13405 166 - 195 1.3e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 674
AccII CGCG 1 cut(s) 733
AciI CCGC 2 cut(s) 131, 731
AclI AACGTT 1 cut(s) 752
AclWI GGATC 3 cut(s) 110, 722, 735
AcoI YGGCCR 2 cut(s) 132, 202
AcsI RAATTY 1 cut(s) 467
AcyI GRCGYC 1 cut(s) 646
AfaI GTAC 1 cut(s) 629
AgsI TTSAA 5 cut(s) 111, 424, 515, 611, 718
AjnI CCWGG 1 cut(s) 623
AluBI AGCT 2 cut(s) 303, 362
AluI AGCT 2 cut(s) 303, 362
Alw21I GWGCWC 1 cut(s) 78
Alw26I GTCTC 1 cut(s) 240
AlwI GGATC 3 cut(s) 110, 722, 735
AoxI GGCC 3 cut(s) 132, 202, 229
ApaI GGGCCC 1 cut(s) 233
ApeKI GCWGC 3 cut(s) 41, 300, 473
ApoI RAATTY 1 cut(s) 467
AspS9I GGNCC 4 cut(s) 229, 230, 396, 458
AsuC2I CCSGG 2 cut(s) 276, 287
AsuHPI GGTGA 1 cut(s) 506
AvaII GGWCC 2 cut(s) 396, 458
AxyI CCTNAGG 2 cut(s) 392, 462
BaeGI GKGCMC 1 cut(s) 233
BamHI GGATCC 1 cut(s) 727
BanII GRGCYC 1 cut(s) 233
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 28, 312, 460
BccI CCATC 3 cut(s) 61, 304, 629
BceAI ACGGC 1 cut(s) 217
BciT130I CCWGG 1 cut(s) 625
BcnI CCSGG 2 cut(s) 276, 287
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 2 cut(s) 359, 581
BisI GCNGC 4 cut(s) 42, 132, 301, 474
BlsI GCNGC 4 cut(s) 43, 133, 302, 475
Bme1390I CCNGG 3 cut(s) 276, 287, 625
Bme18I GGWCC 2 cut(s) 396, 458
BmgT120I GGNCC 4 cut(s) 229, 230, 396, 458
BmiI GGNNCC 3 cut(s) 231, 280, 729
BmrFI CCNGG 3 cut(s) 276, 287, 625
BmsI GCATC 1 cut(s) 556
BpmI CTGGAG 2 cut(s) 502, 575
Bpu10I CCTNAGC 1 cut(s) 72
BpuMI CCSGG 2 cut(s) 276, 287
BsaHI GRCGYC 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 745
Bse21I CCTNAGG 2 cut(s) 392, 462
BseBI CCWGG 1 cut(s) 625
BseGI GGATG 2 cut(s) 53, 693
BseMII CTCAG 4 cut(s) 63, 406, 476, 699
BseNI ACTGG 1 cut(s) 745
BseRI GAGGAG 2 cut(s) 111, 114
BseSI GKGCMC 1 cut(s) 233
BseXI GCAGC 3 cut(s) 28, 312, 460
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 733
BshFI GGCC 3 cut(s) 134, 204, 231
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 2 cut(s) 275, 287
BslFI GGGAC 1 cut(s) 578
BsmAI GTCTC 1 cut(s) 240
BsmBI CGTCTC 1 cut(s) 240
BsmFI GGGAC 1 cut(s) 578
BsnI GGCC 3 cut(s) 134, 204, 231
Bsp120I GGGCCC 1 cut(s) 229
Bsp1286I GDGCHC 2 cut(s) 78, 233
Bsp143I GATC 2 cut(s) 115, 727
BspACI CCGC 2 cut(s) 131, 731
BspANI GGCC 3 cut(s) 134, 204, 231
BspCNI CTCAG 4 cut(s) 64, 405, 475, 700
BspFNI CGCG 1 cut(s) 733
BspLI GGNNCC 3 cut(s) 231, 280, 729
BspPI GGATC 3 cut(s) 110, 722, 735
BsrI ACTGG 1 cut(s) 745
BssMI GATC 2 cut(s) 115, 727
BssNI GRCGYC 1 cut(s) 646
Bst2UI CCWGG 1 cut(s) 625
Bst4CI ACNGT 3 cut(s) 573, 604, 671
Bst6I CTCTTC 1 cut(s) 425
BstACI GRCGYC 1 cut(s) 646
BstC8I GCNNGC 3 cut(s) 161, 407, 588
BstDEI CTNAG 4 cut(s) 72, 392, 462, 708
BstF5I GGATG 2 cut(s) 53, 693
BstFNI CGCG 1 cut(s) 733
BstKTI GATC 2 cut(s) 118, 730
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 2 cut(s) 115, 727
BstMWI GCNNNNNNNGC 1 cut(s) 47
BstNI CCWGG 1 cut(s) 625
BstSCI CCNGG 3 cut(s) 274, 285, 623
BstSLI GKGCMC 1 cut(s) 233
BstUI CGCG 1 cut(s) 733
BstV1I GCAGC 3 cut(s) 28, 312, 460
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
Bsu36I CCTNAGG 2 cut(s) 392, 462
BsuRI GGCC 3 cut(s) 134, 204, 231
BtsCI GGATG 2 cut(s) 53, 693
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 3 cut(s) 161, 407, 588
Cfr13I GGNCC 4 cut(s) 229, 230, 396, 458
CseI GACGC 1 cut(s) 635
Csp6I GTAC 1 cut(s) 628
CviAII CATG 1 cut(s) 508
CviQI GTAC 1 cut(s) 628
DdeI CTNAG 4 cut(s) 72, 392, 462, 708
DpnI GATC 2 cut(s) 117, 729
DpnII GATC 2 cut(s) 115, 727
DrdI GACNNNNNNGTC 1 cut(s) 674
DseDI GACNNNNNNGTC 1 cut(s) 674
EaeI YGGCCR 2 cut(s) 132, 202
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Eco24I GRGCYC 1 cut(s) 233
Eco47I GGWCC 2 cut(s) 396, 458
Eco81I CCTNAGG 2 cut(s) 392, 462
EcoO109I RGGNCCY 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 233
Esp3I CGTCTC 1 cut(s) 240
FaeI CATG 1 cut(s) 511
FaqI GGGAC 1 cut(s) 578
FatI CATG 1 cut(s) 507
FauNDI CATATG 1 cut(s) 247
Fnu4HI GCNGC 4 cut(s) 42, 132, 301, 474
FokI GGATG 2 cut(s) 40, 700
FriOI GRGCYC 1 cut(s) 233
Fsp4HI GCNGC 4 cut(s) 42, 132, 301, 474
FspBI CTAG 2 cut(s) 359, 581
GluI GCNGC 4 cut(s) 42, 132, 301, 474
GsuI CTGGAG 2 cut(s) 502, 575
HaeIII GGCC 3 cut(s) 134, 204, 231
HapII CCGG 2 cut(s) 275, 287
HgaI GACGC 1 cut(s) 635
Hin1I GRCGYC 1 cut(s) 646
Hin1II CATG 1 cut(s) 511
HinfI GANTC 2 cut(s) 65, 254
HpaII CCGG 2 cut(s) 275, 287
HphI GGTGA 1 cut(s) 506
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 5 cut(s) 258, 394, 431, 464, 481
Hpy99I CGWCG 1 cut(s) 651
HpyAV CCTTC 2 cut(s) 249, 718
HpyCH4III ACNGT 3 cut(s) 573, 604, 671
HpyCH4IV ACGT 1 cut(s) 752
HpyCH4V TGCA 4 cut(s) 355, 476, 569, 590
HpyF10VI GCNNNNNNNGC 1 cut(s) 47
HpyF3I CTNAG 4 cut(s) 72, 392, 462, 708
HpySE526I ACGT 1 cut(s) 752
Hsp92I GRCGYC 1 cut(s) 646
Hsp92II CATG 1 cut(s) 511
Kzo9I GATC 2 cut(s) 115, 727
Lsp1109I GCAGC 3 cut(s) 28, 312, 460
LweI GCATC 1 cut(s) 556
MaeI CTAG 2 cut(s) 359, 581
MaeII ACGT 1 cut(s) 752
MaeIII GTNAC 4 cut(s) 185, 197, 209, 758
MalI GATC 2 cut(s) 117, 729
MboI GATC 2 cut(s) 115, 727
MboII GAAGA 6 cut(s) 234, 360, 412, 553, 599, 644
MflI RGATCY 1 cut(s) 727
MhlI GDGCHC 2 cut(s) 78, 233
MluCI AATT 3 cut(s) 139, 350, 467
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 8 cut(s) 129, 132, 135, 220, 254, 401, 471, 606
MspI CCGG 2 cut(s) 275, 287
MspR9I CCNGG 3 cut(s) 276, 287, 625
MvaI CCWGG 1 cut(s) 625
MvnI CGCG 1 cut(s) 733
MwoI GCNNNNNNNGC 1 cut(s) 47
NciI CCSGG 2 cut(s) 276, 287
NdeI CATATG 1 cut(s) 247
NdeII GATC 2 cut(s) 115, 727
NlaIII CATG 1 cut(s) 511
NlaIV GGNNCC 3 cut(s) 231, 280, 729
PfeI GAWTC 1 cut(s) 254
PkrI GCNGC 4 cut(s) 43, 133, 302, 475
PleI GAGTC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 59
Psp1406I AACGTT 1 cut(s) 752
Psp6I CCWGG 1 cut(s) 623
PspGI CCWGG 1 cut(s) 623
PspN4I GGNNCC 3 cut(s) 231, 280, 729
PspOMI GGGCCC 1 cut(s) 229
PspPI GGNCC 4 cut(s) 229, 230, 396, 458
PsuI RGATCY 1 cut(s) 727
RsaI GTAC 1 cut(s) 629
RsaNI GTAC 1 cut(s) 628
SatI GCNGC 4 cut(s) 42, 132, 301, 474
Sau3AI GATC 2 cut(s) 115, 727
Sau96I GGNCC 4 cut(s) 229, 230, 396, 458
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 3 cut(s) 276, 287, 625
SduI GDGCHC 2 cut(s) 78, 233
SetI ASST 5 cut(s) 107, 305, 364, 629, 755
SfaNI GCATC 1 cut(s) 556
SinI GGWCC 2 cut(s) 396, 458
Sse9I AATT 3 cut(s) 139, 350, 467
SsiI CCGC 2 cut(s) 131, 731
SspI AATATT 1 cut(s) 92
SspMI CTAG 2 cut(s) 359, 581
StyD4I CCNGG 3 cut(s) 274, 285, 623
TaaI ACNGT 3 cut(s) 573, 604, 671
TaiI ACGT 1 cut(s) 755
TaqI TCGA 2 cut(s) 660, 678
TaqII GACCGA 1 cut(s) 446
TasI AATT 3 cut(s) 139, 350, 467
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 254
TscAI CASTG 1 cut(s) 576
TseI GCWGC 3 cut(s) 41, 300, 473
TspDTI ATGAA 3 cut(s) 234, 325, 363
TspRI CASTG 1 cut(s) 576
VpaK11BI GGWCC 2 cut(s) 396, 458
XapI RAATTY 1 cut(s) 467
XspI CTAG 2 cut(s) 359, 581
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.