MD11G1164500.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011144
Physical Location & Seq
Reverse (-)
16964551 .. 16966158
1608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1164500.v1.1.491

Sequence Viewer

Length: 540 bp
ATGGCTTCCAATGGGAAATACGGCTCTCACCCTTACGCGCCCTCTGCGCCATACCTGCCCGAGTCCTACGGGCAGCAGCACACCCAATACCCCCATGGTTACGGGCAGCCCCAACAGCAGCATAATTCGTCGTCTTCTTCTCCCCAGTACTCCCATGGTTACGGGCAGCCGCAGCACCAGCCATATGGGTCGTCTTTTTCTCATTCTTCTTCTTCTTCATATGGGCAGTCGGGTTTTCCCGCAGGGACCGACCCGGCCGTGATTAGAAGCTTTGAGATGGTGGACAGGGACAGGAGGGCCGAAGGAGTTTGCTGCACTCTGGACTTGTCTTGGACAATGGCGAGGTTTAGGGTGCCTTCGTCCATATTCGAGAGGTATGACAAAGATAGGAGTGGAAAGATTGATTCTATGGAACTGAGAGATGCACTCTACGGTCTAGGCCTTGCCATGCCACCTTCTGCTCTTCAACTTTTGATTTCCAAATATGACGATGGAAGCGGGCGCAGGGTTGAACTCAACTTTGACAGTTTTGTCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

180

Amino Acids

19.85

Weight (kDa)

6.14

Isoelectric Point (pI)

63.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 121 - 147 7.5e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 530
Acc36I ACCTGC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 352
AccII CGCG 1 cut(s) 38
AciI CCGC 3 cut(s) 170, 240, 498
AcoI YGGCCR 1 cut(s) 255
AfaI GTAC 1 cut(s) 149
AgsI TTSAA 2 cut(s) 467, 512
AluBI AGCT 1 cut(s) 270
AluI AGCT 1 cut(s) 270
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 3 cut(s) 255, 297, 439
ApeKI GCWGC 7 cut(s) 73, 76, 106, 118, 166, 172, 312
AspLEI GCGC 3 cut(s) 40, 49, 504
AspS9I GGNCC 2 cut(s) 246, 297
AsuC2I CCSGG 1 cut(s) 254
AsuHPI GGTGA 1 cut(s) 20
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 1 cut(s) 246
BanI GGYRCC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 7 cut(s) 85, 88, 118, 130, 178, 184, 299
BccI CCATC 2 cut(s) 271, 485
BceAI ACGGC 2 cut(s) 37, 242
BcnI CCSGG 1 cut(s) 254
BfaI CTAG 1 cut(s) 437
BfuAI ACCTGC 1 cut(s) 63
BisI GCNGC 8 cut(s) 74, 77, 107, 119, 167, 170, 173, 313
BlsI GCNGC 8 cut(s) 75, 78, 108, 120, 168, 171, 174, 314
BmcAI AGTACT 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 254
Bme18I GGWCC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 2 cut(s) 246, 297
BmiI GGNNCC 2 cut(s) 247, 354
BmrFI CCNGG 1 cut(s) 254
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 412
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 126
BplI GAGNNNNNCTC 2 cut(s) 411, 443
BpuMI CCSGG 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 94, 154
Bse1I ACTGG 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 94, 154
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 1 cut(s) 145
BseX3I CGGCCG 1 cut(s) 255
BseXI GCAGC 7 cut(s) 85, 88, 118, 130, 178, 184, 299
BsgI GTGCAG 1 cut(s) 298
Bsh1236I CGCG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 258
BshFI GGCC 3 cut(s) 257, 299, 441
BshNI GGYRCC 1 cut(s) 352
BsiEI CGRYCG 1 cut(s) 258
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 1 cut(s) 254
BslFI GGGAC 2 cut(s) 259, 302
BsmFI GGGAC 2 cut(s) 259, 302
BsnI GGCC 3 cut(s) 257, 299, 441
BsoBI CYCGRG 1 cut(s) 59
Bsp19I CCATGG 2 cut(s) 94, 154
BspACI CCGC 3 cut(s) 170, 240, 498
BspANI GGCC 3 cut(s) 257, 299, 441
BspCNI CTCAG 1 cut(s) 408
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 247, 354
BspMI ACCTGC 1 cut(s) 63
BspQI GCTCTTC 1 cut(s) 468
BspT107I GGYRCC 1 cut(s) 352
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 94, 154
BssT1I CCWWGG 2 cut(s) 94, 154
Bst4CI ACNGT 2 cut(s) 434, 527
Bst6I CTCTTC 1 cut(s) 468
BstC8I GCNNGC 1 cut(s) 500
BstDEI CTNAG 1 cut(s) 416
BstDSI CCRYGG 2 cut(s) 94, 154
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 3 cut(s) 40, 49, 504
BstMCI CGRYCG 1 cut(s) 258
BstMWI GCNNNNNNNGC 6 cut(s) 44, 46, 55, 115, 172, 178
BstSCI CCNGG 1 cut(s) 252
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 7 cut(s) 85, 88, 118, 130, 178, 184, 299
BstV2I GAAGAC 1 cut(s) 126
BstXI CCANNNNNNTGG 1 cut(s) 185
BstZI CGGCCG 1 cut(s) 255
BsuRI GGCC 3 cut(s) 257, 299, 441
BtgI CCRYGG 2 cut(s) 94, 154
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 1 cut(s) 500
CfoI GCGC 3 cut(s) 40, 49, 504
Cfr13I GGNCC 2 cut(s) 246, 297
Csp6I GTAC 1 cut(s) 148
CviAII CATG 3 cut(s) 95, 155, 448
CviJI RGCY 9 cut(s) 5, 24, 109, 169, 181, 257, 270, 299, 441
CviKI_1 RGCY 9 cut(s) 5, 24, 109, 169, 181, 257, 270, 299, 441
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 1 cut(s) 416
DrdI GACNNNNNNGTC 1 cut(s) 530
DseDI GACNNNNNNGTC 1 cut(s) 530
EaeI YGGCCR 1 cut(s) 255
EagI CGGCCG 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 468
EarI CTCTTC 1 cut(s) 468
EclXI CGGCCG 1 cut(s) 255
Eco130I CCWWGG 2 cut(s) 94, 154
Eco147I AGGCCT 1 cut(s) 441
Eco47I GGWCC 1 cut(s) 246
Eco52I CGGCCG 1 cut(s) 255
Eco88I CYCGRG 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 94, 154
ErhI CCWWGG 2 cut(s) 94, 154
FaeI CATG 3 cut(s) 98, 158, 451
FaqI GGGAC 2 cut(s) 259, 302
FatI CATG 3 cut(s) 94, 154, 447
FauI CCCGC 2 cut(s) 247, 491
FauNDI CATATG 2 cut(s) 184, 220
Fnu4HI GCNGC 8 cut(s) 74, 77, 107, 119, 167, 170, 173, 313
Fsp4HI GCNGC 8 cut(s) 74, 77, 107, 119, 167, 170, 173, 313
FspBI CTAG 1 cut(s) 437
GlaI GCGC 3 cut(s) 39, 48, 503
GluI GCNGC 8 cut(s) 74, 77, 107, 119, 167, 170, 173, 313
HaeIII GGCC 3 cut(s) 257, 299, 441
HapII CCGG 1 cut(s) 254
HhaI GCGC 3 cut(s) 40, 49, 504
Hin1II CATG 3 cut(s) 98, 158, 451
Hin6I GCGC 3 cut(s) 38, 47, 502
HinP1I GCGC 3 cut(s) 38, 47, 502
HindIII AAGCTT 1 cut(s) 268
HinfI GANTC 2 cut(s) 62, 404
HpaII CCGG 1 cut(s) 254
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 1 cut(s) 283
Hpy188III TCNNGA 2 cut(s) 320, 370
Hpy8I GTNNAC 1 cut(s) 283
Hpy99I CGWCG 1 cut(s) 133
HpyAV CCTTC 3 cut(s) 296, 366, 465
HpyCH4III ACNGT 2 cut(s) 434, 527
HpyCH4V TGCA 2 cut(s) 315, 425
HpyF10VI GCNNNNNNNGC 6 cut(s) 44, 46, 55, 115, 172, 178
HpyF3I CTNAG 1 cut(s) 416
Hsp92II CATG 3 cut(s) 98, 158, 451
HspAI GCGC 3 cut(s) 38, 47, 502
LguI GCTCTTC 1 cut(s) 468
LpnPI CCDG 9 cut(s) 68, 158, 191, 228, 267, 271, 277, 305, 490
Lsp1109I GCAGC 7 cut(s) 85, 88, 118, 130, 178, 184, 299
LweI GCATC 1 cut(s) 412
MaeI CTAG 1 cut(s) 437
MaeIII GTNAC 2 cut(s) 98, 158
MboII GAAGA 7 cut(s) 126, 129, 198, 201, 204, 207, 455
MluCI AATT 1 cut(s) 124
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 4 cut(s) 52, 288, 336, 366
MspI CCGG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 254
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 6 cut(s) 44, 46, 55, 115, 172, 178
NciI CCSGG 1 cut(s) 254
NcoI CCATGG 2 cut(s) 94, 154
NdeI CATATG 2 cut(s) 184, 220
NlaIII CATG 3 cut(s) 98, 158, 451
NlaIV GGNNCC 2 cut(s) 247, 354
PceI AGGCCT 1 cut(s) 441
PciSI GCTCTTC 1 cut(s) 468
PfeI GAWTC 1 cut(s) 404
PkrI GCNGC 8 cut(s) 75, 78, 108, 120, 168, 171, 174, 314
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 247, 354
PspPI GGNCC 2 cut(s) 246, 297
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SapI GCTCTTC 1 cut(s) 468
SatI GCNGC 8 cut(s) 74, 77, 107, 119, 167, 170, 173, 313
Sau96I GGNCC 2 cut(s) 246, 297
ScaI AGTACT 1 cut(s) 149
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 254
SetI ASST 5 cut(s) 57, 272, 347, 377, 457
SfaNI GCATC 1 cut(s) 412
SinI GGWCC 1 cut(s) 246
Sse9I AATT 1 cut(s) 124
SseBI AGGCCT 1 cut(s) 441
SsiI CCGC 3 cut(s) 170, 240, 498
SspMI CTAG 1 cut(s) 437
StuI AGGCCT 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 252
StyI CCWWGG 2 cut(s) 94, 154
TaaI ACNGT 2 cut(s) 434, 527
TaqI TCGA 2 cut(s) 369, 534
TaqII GACCGA 1 cut(s) 263
TasI AATT 1 cut(s) 124
TatI WGTACW 1 cut(s) 147
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 1 cut(s) 404
TseI GCWGC 7 cut(s) 73, 76, 106, 118, 166, 172, 312
TspDTI ATGAA 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 246
XcmI CCANNNNNNNNNTGG 2 cut(s) 92, 152
XspI CTAG 1 cut(s) 437
ZrmI AGTACT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.