MD00G1075300.v1.1

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0011144
Physical Location & Seq
Forward (+)
15206959 .. 15209453
2495 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1075300.v1.1.491

Sequence Viewer

Length: 768 bp
ATGGCTTCCAATGGGAAATACGGCTCTCACCCTTACGCGCCCTCTGCGCCATACCTGCCCGAGTCCTACGGGCAGCAGCACACCCAATACCCCCATGGTTACGGGCAGCCCCAACAGCAGCATAATTCGTCGTCTTCTTCTCCCCAGTACTCCCATGGTTACGGGCAGCCGCAGCACCAGCCATATGGGTCGTCTTTTTCTCATTCTTCTTCTTCATATGGGCAGTCGGGTTTTCCCGCAGGGACCGACCCGGCCGTGATTAGAAGCTTTGAGATGGTGGACAGGGACAGGAGTGGGTACATCGACGAGCACGAGCTGCAGCAAGCTCTGTCTTCTGGGTACCAGAGGTTTAGCCTCAGGACCATTCGCCTGCTCATTTTTCTCTTCAAAAGTCCCACGGATTCTCTCAAAGTCGGGCCGAAGGAGTTTGCTGCACTCTGGACTTGTCTTGGACAATGGCGATCCATATTCGAGAGGTATGACAAAGATAGGAGTGGAAAGATTGATTCTATGGAACTGAGAGATGCACTCTACGGTCTAGGCCTTGCCATGCCACCTTCTGCTCTTCAACTTTTGATTTCCAAATATGACGATGGAAGCGGGCGCAGGGTTGAACTCAACTTTGACAGTTTTGTCGAATGCGGGATGATTGTGAGGGGTTTGACCGATAGGTTCAAAGAGAAAGATCGGCGGTATACTGGTTCGGCAACTTTTGATTACGACGAATTTATGTCTATGGTCATCCCATTTCTTGTGTCGTATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

256

Amino Acids

28.81

Weight (kDa)

6.44

Isoelectric Point (pI)

54.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_6 PF13405 152 - 181 6.9e-07 EF-hand domain
EF-hand_1 PF00036 153 - 178 9.4e-06 EF hand domain
EF-hand_7 PF13499 153 - 206 8.1e-06 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 632
Acc36I ACCTGC 1 cut(s) 63
Acc65I GGTACC 1 cut(s) 339
AccB1I GGYRCC 1 cut(s) 339
AccI GTMKAC 1 cut(s) 695
AccII CGCG 1 cut(s) 38
AciI CCGC 5 cut(s) 170, 237, 600, 642, 691
AclWI GGATC 1 cut(s) 456
AcoI YGGCCR 1 cut(s) 252
AcsI RAATTY 1 cut(s) 725
AfaI GTAC 3 cut(s) 149, 299, 341
AgsI TTSAA 4 cut(s) 388, 569, 614, 676
AluBI AGCT 3 cut(s) 267, 316, 326
AluI AGCT 3 cut(s) 267, 316, 326
Alw21I GWGCWC 1 cut(s) 312
AlwI GGATC 1 cut(s) 456
Ama87I CYCGRG 1 cut(s) 59
AoxI GGCC 3 cut(s) 252, 416, 541
ApeKI GCWGC 9 cut(s) 73, 76, 106, 118, 166, 172, 316, 319, 431
ApoI RAATTY 1 cut(s) 725
Asp718I GGTACC 1 cut(s) 339
AspLEI GCGC 3 cut(s) 40, 49, 606
AspS9I GGNCC 3 cut(s) 243, 360, 416
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 1 cut(s) 20
AvaI CYCGRG 1 cut(s) 59
AvaII GGWCC 2 cut(s) 243, 360
AxyI CCTNAGG 1 cut(s) 356
BanI GGYRCC 1 cut(s) 339
BauI CACGAG 1 cut(s) 311
BbsI GAAGAC 2 cut(s) 126, 324
Bbv12I GWGCWC 1 cut(s) 312
BbvI GCAGC 9 cut(s) 85, 88, 118, 130, 178, 184, 303, 331, 418
BccI CCATC 2 cut(s) 268, 587
BceAI ACGGC 2 cut(s) 37, 239
BcnI CCSGG 1 cut(s) 251
BfaI CTAG 1 cut(s) 539
BfmI CTRYAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 63
BmcAI AGTACT 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 251
Bme18I GGWCC 2 cut(s) 243, 360
BmeT110I CYCGRG 1 cut(s) 59
BmgT120I GGNCC 3 cut(s) 243, 360, 416
BmiI GGNNCC 2 cut(s) 244, 341
BmrFI CCNGG 1 cut(s) 251
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 2 cut(s) 126, 324
BplI GAGNNNNNCTC 2 cut(s) 513, 545
BpuMI CCSGG 1 cut(s) 251
BsaJI CCNNGG 3 cut(s) 94, 154, 396
Bse1I ACTGG 2 cut(s) 145, 703
Bse21I CCTNAGG 1 cut(s) 356
BseDI CCNNGG 3 cut(s) 94, 154, 396
BseGI GGATG 2 cut(s) 651, 741
BseMII CTCAG 2 cut(s) 370, 509
BseNI ACTGG 2 cut(s) 145, 703
BseX3I CGGCCG 1 cut(s) 252
BseXI GCAGC 9 cut(s) 85, 88, 118, 130, 178, 184, 303, 331, 418
BsgI GTGCAG 1 cut(s) 417
Bsh1236I CGCG 1 cut(s) 38
Bsh1285I CGRYCG 1 cut(s) 255
BshFI GGCC 3 cut(s) 254, 418, 543
BshNI GGYRCC 1 cut(s) 339
BsiEI CGRYCG 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 59
BsiSI CCGG 1 cut(s) 251
BslFI GGGAC 3 cut(s) 256, 299, 378
BsmFI GGGAC 3 cut(s) 256, 299, 378
BsmI GAATGC 1 cut(s) 644
BsnI GGCC 3 cut(s) 254, 418, 543
BsoBI CYCGRG 1 cut(s) 59
Bsp1286I GDGCHC 1 cut(s) 312
Bsp143I GATC 2 cut(s) 461, 685
Bsp19I CCATGG 2 cut(s) 94, 154
BspACI CCGC 5 cut(s) 170, 237, 600, 642, 691
BspANI GGCC 3 cut(s) 254, 418, 543
BspCNI CTCAG 2 cut(s) 369, 510
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 2 cut(s) 244, 341
BspMAI CTGCAG 1 cut(s) 321
BspMI ACCTGC 1 cut(s) 63
BspPI GGATC 1 cut(s) 456
BspQI GCTCTTC 1 cut(s) 570
BspT107I GGYRCC 1 cut(s) 339
BsrI ACTGG 2 cut(s) 145, 703
BssECI CCNNGG 3 cut(s) 94, 154, 396
BssMI GATC 2 cut(s) 461, 685
BssNAI GTATAC 1 cut(s) 696
BssSI CACGAG 1 cut(s) 311
BssT1I CCWWGG 2 cut(s) 94, 154
Bst1107I GTATAC 1 cut(s) 696
Bst2BI CACGAG 1 cut(s) 311
Bst4CI ACNGT 2 cut(s) 536, 629
Bst6I CTCTTC 2 cut(s) 389, 570
BstAPI GCANNNNNTGC 1 cut(s) 316
BstC8I GCNNGC 3 cut(s) 324, 371, 602
BstDEI CTNAG 2 cut(s) 356, 518
BstDSI CCRYGG 3 cut(s) 94, 154, 396
BstF5I GGATG 2 cut(s) 651, 741
BstFNI CGCG 1 cut(s) 38
BstHHI GCGC 3 cut(s) 40, 49, 606
BstKTI GATC 2 cut(s) 464, 688
BstMBI GATC 2 cut(s) 461, 685
BstMCI CGRYCG 1 cut(s) 255
BstMWI GCNNNNNNNGC 7 cut(s) 44, 46, 55, 115, 172, 178, 316
BstSCI CCNGG 1 cut(s) 249
BstSFI CTRYAG 1 cut(s) 317
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 9 cut(s) 85, 88, 118, 130, 178, 184, 303, 331, 418
BstV2I GAAGAC 2 cut(s) 126, 324
BstXI CCANNNNNNTGG 1 cut(s) 185
BstZ17I GTATAC 1 cut(s) 696
BstZI CGGCCG 1 cut(s) 252
Bsu36I CCTNAGG 1 cut(s) 356
BsuRI GGCC 3 cut(s) 254, 418, 543
BtgI CCRYGG 3 cut(s) 94, 154, 396
BtsCI GGATG 2 cut(s) 651, 741
BveI ACCTGC 1 cut(s) 63
Cac8I GCNNGC 3 cut(s) 324, 371, 602
CfoI GCGC 3 cut(s) 40, 49, 606
Cfr13I GGNCC 3 cut(s) 243, 360, 416
Csp6I GTAC 3 cut(s) 148, 298, 340
CviAII CATG 3 cut(s) 95, 155, 550
CviQI GTAC 3 cut(s) 148, 298, 340
DdeI CTNAG 2 cut(s) 356, 518
DpnI GATC 2 cut(s) 463, 687
DpnII GATC 2 cut(s) 461, 685
DrdI GACNNNNNNGTC 1 cut(s) 632
DseDI GACNNNNNNGTC 1 cut(s) 632
EaeI YGGCCR 1 cut(s) 252
EagI CGGCCG 1 cut(s) 252
Eam1104I CTCTTC 2 cut(s) 389, 570
EarI CTCTTC 2 cut(s) 389, 570
EclXI CGGCCG 1 cut(s) 252
Eco130I CCWWGG 2 cut(s) 94, 154
Eco147I AGGCCT 1 cut(s) 543
Eco47I GGWCC 2 cut(s) 243, 360
Eco52I CGGCCG 1 cut(s) 252
Eco81I CCTNAGG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 59
EcoT14I CCWWGG 2 cut(s) 94, 154
ErhI CCWWGG 2 cut(s) 94, 154
FaeI CATG 3 cut(s) 98, 158, 553
FaqI GGGAC 3 cut(s) 256, 299, 378
FatI CATG 3 cut(s) 94, 154, 549
FauI CCCGC 3 cut(s) 244, 593, 635
FauNDI CATATG 2 cut(s) 184, 217
FblI GTMKAC 1 cut(s) 695
FokI GGATG 2 cut(s) 658, 728
FspBI CTAG 1 cut(s) 539
GlaI GCGC 3 cut(s) 39, 48, 605
HaeIII GGCC 3 cut(s) 254, 418, 543
HapII CCGG 1 cut(s) 251
HhaI GCGC 3 cut(s) 40, 49, 606
Hin1II CATG 3 cut(s) 98, 158, 553
Hin6I GCGC 3 cut(s) 38, 47, 604
HinP1I GCGC 3 cut(s) 38, 47, 604
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 3 cut(s) 62, 401, 506
HpaII CCGG 1 cut(s) 251
HphI GGTGA 1 cut(s) 20
Hpy166II GTNNAC 2 cut(s) 280, 696
Hpy188III TCNNGA 3 cut(s) 358, 439, 472
Hpy8I GTNNAC 2 cut(s) 280, 696
Hpy99I CGWCG 3 cut(s) 133, 308, 725
HpyAV CCTTC 2 cut(s) 415, 567
HpyCH4III ACNGT 2 cut(s) 536, 629
HpyCH4V TGCA 3 cut(s) 319, 434, 527
HpyF10VI GCNNNNNNNGC 7 cut(s) 44, 46, 55, 115, 172, 178, 316
HpyF3I CTNAG 2 cut(s) 356, 518
Hsp92II CATG 3 cut(s) 98, 158, 553
HspAI GCGC 3 cut(s) 38, 47, 604
KpnI GGTACC 1 cut(s) 343
Kzo9I GATC 2 cut(s) 461, 685
LguI GCTCTTC 1 cut(s) 570
Lsp1109I GCAGC 9 cut(s) 85, 88, 118, 130, 178, 184, 303, 331, 418
LweI GCATC 1 cut(s) 514
MaeI CTAG 1 cut(s) 539
MaeIII GTNAC 2 cut(s) 98, 158
MalI GATC 2 cut(s) 463, 687
MboI GATC 2 cut(s) 461, 685
MboII GAAGA 8 cut(s) 126, 129, 198, 201, 204, 324, 376, 557
MhlI GDGCHC 1 cut(s) 312
MluCI AATT 3 cut(s) 124, 725, 763
MlyI GAGTC 1 cut(s) 71
MnlI CCTC 5 cut(s) 52, 339, 365, 468, 648
MspI CCGG 1 cut(s) 251
MspR9I CCNGG 1 cut(s) 251
Mva1269I GAATGC 1 cut(s) 644
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 7 cut(s) 44, 46, 55, 115, 172, 178, 316
NciI CCSGG 1 cut(s) 251
NcoI CCATGG 2 cut(s) 94, 154
NdeI CATATG 2 cut(s) 184, 217
NdeII GATC 2 cut(s) 461, 685
NlaIII CATG 3 cut(s) 98, 158, 553
NlaIV GGNNCC 2 cut(s) 244, 341
PceI AGGCCT 1 cut(s) 543
PciSI GCTCTTC 1 cut(s) 570
PcsI WCGNNNNNNNCGW 1 cut(s) 309
PctI GAATGC 1 cut(s) 644
PfeI GAWTC 2 cut(s) 401, 506
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 244, 341
PspPI GGNCC 3 cut(s) 243, 360, 416
PstI CTGCAG 1 cut(s) 321
RsaI GTAC 3 cut(s) 149, 299, 341
RsaNI GTAC 3 cut(s) 148, 298, 340
SapI GCTCTTC 1 cut(s) 570
Sau3AI GATC 2 cut(s) 461, 685
Sau96I GGNCC 3 cut(s) 243, 360, 416
ScaI AGTACT 1 cut(s) 149
SchI GAGTC 1 cut(s) 71
ScrFI CCNGG 1 cut(s) 251
SduI GDGCHC 1 cut(s) 312
SetI ASST 8 cut(s) 57, 269, 318, 328, 350, 479, 559, 674
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 317
SinI GGWCC 2 cut(s) 243, 360
Sse9I AATT 3 cut(s) 124, 725, 763
SseBI AGGCCT 1 cut(s) 543
SsiI CCGC 5 cut(s) 170, 237, 600, 642, 691
SspMI CTAG 1 cut(s) 539
StuI AGGCCT 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 2 cut(s) 94, 154
TaaI ACNGT 2 cut(s) 536, 629
TaqI TCGA 3 cut(s) 303, 471, 636
TaqII GACCGA 2 cut(s) 260, 680
TasI AATT 3 cut(s) 124, 725, 763
TatI WGTACW 1 cut(s) 147
TauI GCSGC 1 cut(s) 172
TfiI GAWTC 2 cut(s) 401, 506
TseI GCWGC 9 cut(s) 73, 76, 106, 118, 166, 172, 316, 319, 431
TspDTI ATGAA 1 cut(s) 204
TspGWI ACGGA 1 cut(s) 413
VpaK11BI GGWCC 2 cut(s) 243, 360
XapI RAATTY 1 cut(s) 725
XcmI CCANNNNNNNNNTGG 2 cut(s) 92, 152
XmiI GTMKAC 1 cut(s) 695
XspI CTAG 1 cut(s) 539
ZrmI AGTACT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.