FvH4_3g24940

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011144
Physical Location & Seq
Reverse (-)
17987197 .. 17989768
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g24940.t1

Sequence Viewer

Length: 843 bp
ATGGCTTTTTCTGATGCCAACTACGGTTCTGATACCTACGCTCCCTCTGCCCCATCCCTACCCGACTCCTACGGCCACCAGGAACCAGAAGTCACCGTTTCTGATAATGATCCTCCTCCTCCTCCTACGGCGAATACATATTACTACGGCAAGCCGCACTCGCAATACGGCCGCAGTTACGTCCGTAGTTACGGCCATAGTTACGCCCGTAGTGACGGCCTGCCGGAGCTGGACCTGTCTCCTTCTTCGTATGATCAACCGTCTTCTTCTTCTTCATATGATCAGGAAACAGGTGGGGATTTTCCACCGGGGACCGACCCGGATGTGATTAGCAGCTTTCAGATGGTGGATAAGGACGGTAGTGGCTTCATTGACGACAATGAATTGCAACAGGCTCTTTCTTCTGCCTACCAGATGTTTAGCCTCAGGACCATTCGTTTGCTCATGTTTCTCTTCAAAAACATCAACAATCATGATATTCTAAAACTGGGTCCTCAAGAATTTGCCGCACTATGGAGTTGTCTTGGTGAATGGCGAACCATGTTTCAGAAATATGACACAGATAAGAGTGGAAAGATTGATGTGATAGAGTTGAGAGATGCACTCTTTGGTCTAGGACTTGCAGTCCCCCCTACTGTTCTTCAACTTCTGACTTCCAGGTACGAAGATGGAAGTGGGCACCGGGCTGAACTTAACTTTGATAGTTTTGTCGAATGTGGGATGATTGTAAAGGGCTTGACTGAGAAGTTCAAAGAGAAGGATCGAAAATATACTGGTTCAGCAACATTCAGTTACAACGCATTTATGTCTATCGTCATCCCATTTCTTGTGTCATATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

281

Amino Acids

31.2

Weight (kDa)

4.63

Isoelectric Point (pI)

50.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 177 - 202 3.8e-06 EF hand domain
EF-hand_6 PF13405 177 - 206 1.9e-06 EF-hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011144)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G27480 AT2G27480
fragaria_vesca FvH4_3g24940
malus_domestica MD00G1075300.v1.1 MD03G1144700.v1.1 MD11G1164500.v1.1
prunus_persica Prupe.6G130400_v2.0.a1 Prupe.6G130400_v2.0.a1
pyrus_communis pycom11g13680
rosa_chinensis RchiOBHm_Chr5g0044731
rosa_laevigata RLG00000034294
rosa_multiflora Rmu_sc0000228.1_g000011
rosa_roxburghii Rroxscaffold_1G00036300
rosa_rugosa Rorug05G0217200
rosa_samantha Rh5AG300500 Rh5BG307200 Rh5CG334200 Rh5DG317300
rosa_wichuraiana Rw5G027800 Rw5G027840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 678
AciI CCGC 3 cut(s) 155, 172, 507
AclWI GGATC 2 cut(s) 104, 768
AcoI YGGCCR 3 cut(s) 73, 169, 193
AcsI RAATTY 1 cut(s) 500
AfaI GTAC 1 cut(s) 662
AfiI CCNNNNNNNGG 1 cut(s) 513
AgsI TTSAA 3 cut(s) 457, 644, 751
AhdI GACNNNNNGTC 1 cut(s) 623
AjnI CCWGG 2 cut(s) 78, 656
AluBI AGCT 2 cut(s) 229, 336
AluI AGCT 2 cut(s) 229, 336
Alw26I GTCTC 1 cut(s) 243
AlwI GGATC 2 cut(s) 104, 768
AoxI GGCC 4 cut(s) 73, 169, 193, 217
ApeKI GCWGC 1 cut(s) 333
ApoI RAATTY 1 cut(s) 500
ArsI GACNNNNNNTTYG 2 cut(s) 421, 453
AspS9I GGNCC 4 cut(s) 232, 312, 429, 491
AsuC2I CCSGG 3 cut(s) 309, 320, 683
AsuHPI GGTGA 2 cut(s) 85, 539
AvaII GGWCC 4 cut(s) 232, 312, 429, 491
AxyI CCTNAGG 1 cut(s) 425
BaeGI GKGCMC 1 cut(s) 681
BanI GGYRCC 1 cut(s) 678
BbsI GAAGAC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 345
BccI CCATC 3 cut(s) 61, 337, 662
BceAI ACGGC 6 cut(s) 88, 144, 163, 184, 208, 232
BciT130I CCWGG 2 cut(s) 80, 658
BclI TGATCA 2 cut(s) 253, 280
BcnI CCSGG 3 cut(s) 309, 320, 683
BcoDI GTCTC 1 cut(s) 243
BfaI CTAG 1 cut(s) 614
BisI GCNGC 4 cut(s) 155, 172, 334, 507
BlsI GCNGC 4 cut(s) 156, 173, 335, 508
Bme1390I CCNGG 5 cut(s) 80, 309, 320, 658, 683
Bme18I GGWCC 4 cut(s) 232, 312, 429, 491
BmeRI GACNNNNNGTC 1 cut(s) 623
BmgT120I GGNCC 4 cut(s) 232, 312, 429, 491
BmiI GGNNCC 4 cut(s) 84, 313, 492, 680
BmrFI CCNGG 5 cut(s) 80, 309, 320, 658, 683
BmrI ACTGGG 1 cut(s) 497
BmsI GCATC 2 cut(s) 4, 589
BmuI ACTGGG 1 cut(s) 497
BpiI GAAGAC 1 cut(s) 255
BplI GAGNNNNNCTC 2 cut(s) 588, 620
BpuEI CTTGAG 1 cut(s) 480
BpuMI CCSGG 3 cut(s) 309, 320, 683
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 308
BsaXI ACNNNNNCTCC 2 cut(s) 25, 55
Bsc4I CCNNNNNNNGG 1 cut(s) 513
Bse1I ACTGG 2 cut(s) 492, 778
Bse21I CCTNAGG 1 cut(s) 425
Bse8I GATNNNNATC 1 cut(s) 108
BseBI CCWGG 2 cut(s) 80, 658
BseDI CCNNGG 1 cut(s) 308
BseGI GGATG 4 cut(s) 53, 328, 726, 816
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 1 cut(s) 513
BseMII CTCAG 2 cut(s) 439, 732
BseNI ACTGG 2 cut(s) 492, 778
BseRI GAGGAG 3 cut(s) 105, 108, 111
BseSI GKGCMC 1 cut(s) 681
BseX3I CGGCCG 1 cut(s) 169
BseXI GCAGC 1 cut(s) 345
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 4 cut(s) 75, 171, 195, 219
BshNI GGYRCC 1 cut(s) 678
BsiEI CGRYCG 1 cut(s) 172
BsiSI CCGG 4 cut(s) 224, 308, 320, 682
BslFI GGGAC 2 cut(s) 325, 611
BslI CCNNNNNNNGG 1 cut(s) 513
BsmAI GTCTC 1 cut(s) 243
BsmFI GGGAC 2 cut(s) 325, 611
BsnI GGCC 4 cut(s) 75, 171, 195, 219
Bsp1286I GDGCHC 1 cut(s) 681
Bsp143I GATC 4 cut(s) 109, 253, 280, 760
BspACI CCGC 3 cut(s) 155, 172, 507
BspANI GGCC 4 cut(s) 75, 171, 195, 219
BspCNI CTCAG 2 cut(s) 438, 733
BspHI TCATGA 1 cut(s) 472
BspLI GGNNCC 4 cut(s) 84, 313, 492, 680
BspPI GGATC 2 cut(s) 104, 768
BspT107I GGYRCC 1 cut(s) 678
BsrI ACTGG 2 cut(s) 492, 778
BssECI CCNNGG 1 cut(s) 308
BssMI GATC 4 cut(s) 109, 253, 280, 760
Bst2UI CCWGG 2 cut(s) 80, 658
Bst4CI ACNGT 5 cut(s) 26, 97, 261, 359, 637
Bst6I CTCTTC 1 cut(s) 458
BstC8I GCNNGC 2 cut(s) 152, 221
BstDEI CTNAG 2 cut(s) 425, 741
BstF5I GGATG 4 cut(s) 53, 328, 726, 816
BstKTI GATC 4 cut(s) 112, 256, 283, 763
BstMAI GTCTC 1 cut(s) 243
BstMBI GATC 4 cut(s) 109, 253, 280, 760
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 2 cut(s) 47, 160
BstNI CCWGG 2 cut(s) 80, 658
BstSCI CCNGG 5 cut(s) 78, 307, 318, 656, 681
BstSLI GKGCMC 1 cut(s) 681
BstV1I GCAGC 1 cut(s) 345
BstV2I GAAGAC 1 cut(s) 255
BstZI CGGCCG 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 425
BsuRI GGCC 4 cut(s) 75, 171, 195, 219
BtsCI GGATG 4 cut(s) 53, 328, 726, 816
Cac8I GCNNGC 2 cut(s) 152, 221
CciI TCATGA 1 cut(s) 472
Cfr13I GGNCC 4 cut(s) 232, 312, 429, 491
Csp6I GTAC 1 cut(s) 661
CviAII CATG 3 cut(s) 445, 473, 541
CviQI GTAC 1 cut(s) 661
DdeI CTNAG 2 cut(s) 425, 741
DpnI GATC 4 cut(s) 111, 255, 282, 762
DpnII GATC 4 cut(s) 109, 253, 280, 760
DriI GACNNNNNGTC 1 cut(s) 623
EaeI YGGCCR 3 cut(s) 73, 169, 193
EagI CGGCCG 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 458
Eam1105I GACNNNNNGTC 1 cut(s) 623
EarI CTCTTC 1 cut(s) 458
EclXI CGGCCG 1 cut(s) 169
Eco47I GGWCC 4 cut(s) 232, 312, 429, 491
Eco52I CGGCCG 1 cut(s) 169
Eco81I CCTNAGG 1 cut(s) 425
EcoO109I RGGNCCY 1 cut(s) 491
EcoRII CCWGG 2 cut(s) 78, 656
FaeI CATG 3 cut(s) 448, 476, 544
FaqI GGGAC 2 cut(s) 325, 611
FatI CATG 3 cut(s) 444, 472, 540
FauNDI CATATG 1 cut(s) 277
FbaI TGATCA 2 cut(s) 253, 280
Fnu4HI GCNGC 4 cut(s) 155, 172, 334, 507
FokI GGATG 4 cut(s) 40, 335, 733, 803
Fsp4HI GCNGC 4 cut(s) 155, 172, 334, 507
FspBI CTAG 1 cut(s) 614
GluI GCNGC 4 cut(s) 155, 172, 334, 507
HaeIII GGCC 4 cut(s) 75, 171, 195, 219
HapII CCGG 4 cut(s) 224, 308, 320, 682
Hin1II CATG 3 cut(s) 448, 476, 544
HinfI GANTC 1 cut(s) 65
HpaII CCGG 4 cut(s) 224, 308, 320, 682
HphI GGTGA 2 cut(s) 85, 539
Hpy188I TCNGA 6 cut(s) 13, 31, 103, 342, 549, 651
Hpy188III TCNNGA 4 cut(s) 284, 427, 473, 497
HpyAV CCTTC 2 cut(s) 252, 751
HpyCH4III ACNGT 5 cut(s) 26, 97, 261, 359, 637
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 3 cut(s) 388, 602, 623
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 160
HpyF3I CTNAG 2 cut(s) 425, 741
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 3 cut(s) 448, 476, 544
Ksp22I TGATCA 2 cut(s) 253, 280
Kzo9I GATC 4 cut(s) 109, 253, 280, 760
LmnI GCTCC 2 cut(s) 46, 226
Lsp1109I GCAGC 1 cut(s) 345
LweI GCATC 2 cut(s) 4, 589
MaeI CTAG 1 cut(s) 614
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 6 cut(s) 91, 176, 188, 200, 212, 791
MalI GATC 4 cut(s) 111, 255, 282, 762
MboI GATC 4 cut(s) 109, 253, 280, 760
MboII GAAGA 9 cut(s) 237, 255, 258, 261, 264, 393, 445, 632, 677
MhlI GDGCHC 1 cut(s) 681
MluCI AATT 3 cut(s) 383, 500, 838
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 7 cut(s) 55, 123, 126, 129, 132, 434, 504
MseI TTAA 1 cut(s) 693
MspI CCGG 4 cut(s) 224, 308, 320, 682
MspR9I CCNGG 5 cut(s) 80, 309, 320, 658, 683
MvaI CCWGG 2 cut(s) 80, 658
MwoI GCNNNNNNNGC 2 cut(s) 47, 160
NciI CCSGG 3 cut(s) 309, 320, 683
NdeI CATATG 1 cut(s) 277
NdeII GATC 4 cut(s) 109, 253, 280, 760
NlaIII CATG 3 cut(s) 448, 476, 544
NlaIV GGNNCC 4 cut(s) 84, 313, 492, 680
NmuCI GTSAC 2 cut(s) 91, 212
PagI TCATGA 1 cut(s) 472
PkrI GCNGC 4 cut(s) 156, 173, 335, 508
PleI GAGTC 1 cut(s) 59
PpsI GAGTC 1 cut(s) 59
PpuMI RGGWCCY 1 cut(s) 491
Psp5II RGGWCCY 1 cut(s) 491
Psp6I CCWGG 2 cut(s) 78, 656
PspGI CCWGG 2 cut(s) 78, 656
PspN4I GGNNCC 4 cut(s) 84, 313, 492, 680
PspPI GGNCC 4 cut(s) 232, 312, 429, 491
PspPPI RGGWCCY 1 cut(s) 491
RsaI GTAC 1 cut(s) 662
RsaNI GTAC 1 cut(s) 661
SaqAI TTAA 1 cut(s) 693
SatI GCNGC 4 cut(s) 155, 172, 334, 507
Sau3AI GATC 4 cut(s) 109, 253, 280, 760
Sau96I GGNCC 4 cut(s) 232, 312, 429, 491
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 5 cut(s) 80, 309, 320, 658, 683
SduI GDGCHC 1 cut(s) 681
SetI ASST 7 cut(s) 38, 183, 231, 237, 295, 338, 662
SfaNI GCATC 2 cut(s) 4, 589
SinI GGWCC 4 cut(s) 232, 312, 429, 491
SmlI CTYRAG 1 cut(s) 495
SmoI CTYRAG 1 cut(s) 495
Sse9I AATT 3 cut(s) 383, 500, 838
SsiI CCGC 3 cut(s) 155, 172, 507
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 5 cut(s) 78, 307, 318, 656, 681
TaaI ACNGT 5 cut(s) 26, 97, 261, 359, 637
TaiI ACGT 1 cut(s) 183
TaqI TCGA 2 cut(s) 711, 763
TaqII GACCGA 1 cut(s) 329
TasI AATT 3 cut(s) 383, 500, 838
TauI GCSGC 3 cut(s) 157, 174, 509
Tru1I TTAA 1 cut(s) 693
Tru9I TTAA 1 cut(s) 693
TseFI GTSAC 2 cut(s) 91, 212
TseI GCWGC 1 cut(s) 333
Tsp45I GTSAC 2 cut(s) 91, 212
TspDTI ATGAA 3 cut(s) 264, 358, 396
TspGWI ACGGA 1 cut(s) 173
VpaK11BI GGWCC 4 cut(s) 232, 312, 429, 491
XapI RAATTY 1 cut(s) 500
XspI CTAG 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.