AT2G28370

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015903
Physical Location & Seq
Forward (+)
12131544 .. 12133772
2229 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28370.1

Sequence Viewer

Length: 540 bp
ATGAATGTGAGCCATGCATCGGTACATCCAGTGGAAGATCCTCCTGCAGCTGCGACGGAAGTAGAGAATCCGCCTCGGGTGAGGATGGATGATATGGAAGGAATGCCTGGAACATTGCTTGGTCTTGCATTGCGTTTTTTTCAGTTCTTGTTTGCTGCTGCTGCTCTTTGTGTCATGGCTTCTACTAGTGATTTTCCTTCCGTTACCGCCTTCTGCTACCTAGTTGCAGCTACTGGTCTGCAGAGCTTGTGGAGTCTTGCACTAGCCATGGTTGACGTCTATGCCATTATGGTCAAACGCTCTCTACAGAACCGTCGACTTGTGAGCTTGTTTGCAATTGGTGATGGGGTGACATCGACGCTGACTTTTGCAGCAGCTTGTGCATCGGCAGGCATAACGGTTCTAATAGACAACGATCTGAATAGCTGCGCACAAAACCACTGTGTTCAGTTTGAAACATCAACGGCATTAGCTTTCATAAGCTGGTTCGCTGCTTTGCCTTCGTTTCTCTTCAATTTCTGGTCTCTTGCATCCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

179

Amino Acids

19.22

Weight (kDa)

4.92

Isoelectric Point (pI)

39.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 35 - 164 6.3e-30 Casparian strip membrane protein domain
MARVEL PF01284 42 - 170 9.2e-07 Membrane-associating domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28370
fragaria_vesca FvH4_3g32060
malus_domestica MD03G1119100.v1.1 MD11G1136900.v1.1
prunus_persica Prupe.6G102400_v2.0.a1
pyrus_communis pycom11g11350
rosa_chinensis RchiOBHm_Chr5g0058781
rosa_laevigata RLG00000035248
rosa_multiflora Rmu_sc0008874.1_g000008
rosa_roxburghii Rroxscaffold_1G00021620
rosa_rugosa Rorug05G0319100
rosa_samantha Rh5AG383800 Rh5CG419600 Rh5DG411400
rosa_wichuraiana Rw5G036170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 279
Acc16I TGCGCA 1 cut(s) 430
AccI GTMKAC 1 cut(s) 316
AciI CCGC 2 cut(s) 71, 207
AclWI GGATC 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 19
AgsI TTSAA 2 cut(s) 455, 514
AhlI ACTAGT 1 cut(s) 185
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 8 cut(s) 50, 230, 246, 327, 377, 426, 473, 483
AluI AGCT 8 cut(s) 50, 230, 246, 327, 377, 426, 473, 483
Alw26I GTCTC 1 cut(s) 528
AlwI GGATC 1 cut(s) 32
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 1 cut(s) 75
AspLEI GCGC 1 cut(s) 431
AsuHPI GGTGA 3 cut(s) 91, 353, 361
AvaI CYCGRG 1 cut(s) 75
BccI CCATC 2 cut(s) 79, 338
BceAI ACGGC 1 cut(s) 480
BciT130I CCWGG 1 cut(s) 108
BcoDI GTCTC 1 cut(s) 528
BcuI ACTAGT 1 cut(s) 185
BfaI CTAG 3 cut(s) 186, 221, 263
BfmI CTRYAG 3 cut(s) 45, 239, 305
Bme1390I CCNGG 1 cut(s) 108
BmeT110I CYCGRG 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 26, 392
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 74, 267
Bsc4I CCNNNNNNNGG 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 29, 238
Bse3DI GCAATG 2 cut(s) 113, 128
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 2 cut(s) 74, 267
BseGI GGATG 4 cut(s) 25, 90, 94, 530
BseLI CCNNNNNNNGG 1 cut(s) 19
BseMI GCAATG 2 cut(s) 113, 128
BseNI ACTGG 2 cut(s) 29, 238
BsiHKCI CYCGRG 1 cut(s) 75
BslI CCNNNNNNNGG 1 cut(s) 19
BsmAI GTCTC 1 cut(s) 528
BsmI GAATGC 1 cut(s) 108
Bso31I GGTCTC 1 cut(s) 528
BsoBI CYCGRG 1 cut(s) 75
Bsp143I GATC 2 cut(s) 37, 415
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 2 cut(s) 71, 207
BspMAI CTGCAG 2 cut(s) 49, 243
BspPI GGATC 1 cut(s) 32
BspTNI GGTCTC 1 cut(s) 528
BsrDI GCAATG 2 cut(s) 113, 128
BsrI ACTGG 2 cut(s) 29, 238
BssECI CCNNGG 2 cut(s) 74, 267
BssMI GATC 2 cut(s) 37, 415
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 314, 400, 443
Bst6I CTCTTC 1 cut(s) 515
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 380
BstC8I GCNNGC 1 cut(s) 391
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 4 cut(s) 25, 90, 94, 530
BstHHI GCGC 1 cut(s) 431
BstKTI GATC 2 cut(s) 40, 418
BstMAI GTCTC 1 cut(s) 528
BstMBI GATC 2 cut(s) 37, 415
BstMWI GCNNNNNNNGC 2 cut(s) 161, 380
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 3 cut(s) 45, 239, 305
BstX2I RGATCY 1 cut(s) 37
BstYI RGATCY 1 cut(s) 37
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 4 cut(s) 25, 90, 94, 530
BtsIMutI CAGTG 2 cut(s) 36, 439
Cac8I GCNNGC 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 233
CfoI GCGC 1 cut(s) 431
CseI GACGC 1 cut(s) 367
Csp6I GTAC 1 cut(s) 23
CviAII CATG 3 cut(s) 14, 175, 268
CviQI GTAC 1 cut(s) 23
DpnI GATC 2 cut(s) 39, 417
DpnII GATC 2 cut(s) 37, 415
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
EciI GGCGGA 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 267
Eco31I GGTCTC 1 cut(s) 528
Eco88I CYCGRG 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 267
EcoT22I ATGCAT 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 3 cut(s) 17, 178, 271
FaiI YATR 8 cut(s) 15, 95, 176, 269, 282, 290, 395, 479
FatI CATG 3 cut(s) 13, 174, 267
FblI GTMKAC 1 cut(s) 316
FokI GGATG 4 cut(s) 12, 97, 101, 517
FspBI CTAG 3 cut(s) 186, 221, 263
FspI TGCGCA 1 cut(s) 430
GlaI GCGC 1 cut(s) 430
HgaI GACGC 1 cut(s) 367
HhaI GCGC 1 cut(s) 431
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 3 cut(s) 17, 178, 271
Hin6I GCGC 1 cut(s) 429
HinP1I GCGC 1 cut(s) 429
HincII GTYRAC 2 cut(s) 274, 317
HindII GTYRAC 2 cut(s) 274, 317
HinfI GANTC 2 cut(s) 67, 253
HphI GGTGA 3 cut(s) 91, 353, 361
Hpy166II GTNNAC 2 cut(s) 274, 317
Hpy188I TCNGA 1 cut(s) 420
Hpy8I GTNNAC 2 cut(s) 274, 317
Hpy99I CGWCG 3 cut(s) 58, 318, 361
HpyAV CCTTC 4 cut(s) 92, 207, 220, 510
HpyCH4III ACNGT 3 cut(s) 314, 400, 443
HpyCH4IV ACGT 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 380
HpySE526I ACGT 1 cut(s) 276
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 3 cut(s) 17, 178, 271
HspAI GCGC 1 cut(s) 429
Kzo9I GATC 2 cut(s) 37, 415
LpnPI CCDG 8 cut(s) 42, 57, 93, 120, 219, 375, 469, 505
LweI GCATC 2 cut(s) 26, 392
MaeI CTAG 3 cut(s) 186, 221, 263
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 202, 349
MalI GATC 2 cut(s) 39, 417
MboI GATC 2 cut(s) 37, 415
MboII GAAGA 2 cut(s) 47, 502
MfeI CAATTG 1 cut(s) 336
MflI RGATCY 1 cut(s) 37
MluCI AATT 2 cut(s) 336, 514
MlyI GAGTC 1 cut(s) 262
MnlI CCTC 3 cut(s) 51, 75, 84
Mph1103I ATGCAT 1 cut(s) 19
MspA1I CMGCKG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 108
MunI CAATTG 1 cut(s) 336
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
MwoI GCNNNNNNNGC 2 cut(s) 161, 380
NcoI CCATGG 1 cut(s) 267
NdeII GATC 2 cut(s) 37, 415
NlaIII CATG 3 cut(s) 17, 178, 271
NmuCI GTSAC 1 cut(s) 349
NsbI TGCGCA 1 cut(s) 430
NsiI ATGCAT 1 cut(s) 19
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 67
PleI GAGTC 1 cut(s) 261
PpsI GAGTC 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 106
PspGI CCWGG 1 cut(s) 106
PstI CTGCAG 2 cut(s) 49, 243
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 37
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SalI GTCGAC 1 cut(s) 315
Sau3AI GATC 2 cut(s) 37, 415
SchI GAGTC 1 cut(s) 262
ScrFI CCNGG 1 cut(s) 108
SfaNI GCATC 2 cut(s) 26, 392
SfcI CTRYAG 3 cut(s) 45, 239, 305
SpeI ACTAGT 1 cut(s) 185
Sse9I AATT 2 cut(s) 336, 514
SsiI CCGC 2 cut(s) 71, 207
SspMI CTAG 3 cut(s) 186, 221, 263
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 3 cut(s) 314, 400, 443
TaiI ACGT 1 cut(s) 279
TaqI TCGA 2 cut(s) 316, 356
TasI AATT 2 cut(s) 336, 514
TfiI GAWTC 1 cut(s) 67
TscAI CASTG 2 cut(s) 36, 446
TseFI GTSAC 1 cut(s) 349
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 2 cut(s) 17, 466
TspGWI ACGGA 2 cut(s) 71, 190
TspRI CASTG 2 cut(s) 36, 446
XmiI GTMKAC 1 cut(s) 316
XspI CTAG 3 cut(s) 186, 221, 263
ZraI GACGTC 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.