MD11G1136900.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015903
Physical Location & Seq
Reverse (-)
12620771 .. 12622918
2148 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1136900.v1.1.491

Sequence Viewer

Length: 540 bp
ATGAACGTGAGCCATGGGTCGGTTCACCCAGTTGAAGATGTACCCACCACAGAGGGGGCCGGCCCGAACCCGCTCCGGCTCCGAATGAAGGATCTTCCGGGCATGCCCGGCACCCCGGGCGGCCTCGTCTTGCGCCTCGCCCAGTTTTTCTTCTCCGCCGCCGCGCTCGTCGTCATGGCCACCACCTCTGACTTCCCCTCCGTCACCGCGTTTTGCTACCTTGTTGCAGCTACTGGTTTGCAGTGCTTGTGGAGCTTCTCGTTGGGCATTGTTGACGTTTATGCCCTTCTAGTCAGGCGTTCTTTGCAGAATCATGGAGTTGTGTGCTTGTTCACCCTCGGCGATGGGGTGACCTCCACTCTTACATTTGCTGCTGCTTGTGCTTCTGCGGGCATCACGGTTCTCATTGACAATGATCTTGATGGCTGTGCCCGCAACCATTGTGCACAATTTGAAACAGCTACAGCCATGGCTTTCATATCCTGGTTCGCTGCATTACCATCTTTTCTCTTGAATTTCTGGTCACTGGCATCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

180

Amino Acids

18.95

Weight (kDa)

5.67

Isoelectric Point (pI)

37.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 164 2e-28 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28370
fragaria_vesca FvH4_3g32060
malus_domestica MD03G1119100.v1.1 MD11G1136900.v1.1
prunus_persica Prupe.6G102400_v2.0.a1
pyrus_communis pycom11g11350
rosa_chinensis RchiOBHm_Chr5g0058781
rosa_laevigata RLG00000035248
rosa_multiflora Rmu_sc0008874.1_g000008
rosa_roxburghii Rroxscaffold_1G00021620
rosa_rugosa Rorug05G0319100
rosa_samantha Rh5AG383800 Rh5CG419600 Rh5DG411400
rosa_wichuraiana Rw5G036170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 73
AccII CGCG 2 cut(s) 164, 209
AciI CCGC 8 cut(s) 71, 120, 156, 159, 162, 207, 389, 433
AclWI GGATC 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 514
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 3 cut(s) 19, 54, 88
AgsI TTSAA 3 cut(s) 35, 455, 514
AjnI CCWGG 1 cut(s) 482
AluBI AGCT 3 cut(s) 230, 255, 461
AluI AGCT 3 cut(s) 230, 255, 461
Alw21I GWGCWC 1 cut(s) 448
Alw44I GTGCAC 1 cut(s) 444
AlwI GGATC 1 cut(s) 99
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 1 cut(s) 115
AoxI GGCC 4 cut(s) 57, 61, 121, 177
ApaLI GTGCAC 1 cut(s) 444
ApeKI GCWGC 4 cut(s) 227, 371, 374, 491
ApoI RAATTY 1 cut(s) 514
AspLEI GCGC 2 cut(s) 135, 166
AspS9I GGNCC 2 cut(s) 57, 62
AsuC2I CCSGG 4 cut(s) 99, 108, 116, 117
AsuHPI GGTGA 4 cut(s) 17, 196, 325, 361
AvaI CYCGRG 1 cut(s) 115
BaeGI GKGCMC 2 cut(s) 433, 448
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 1 cut(s) 110
Bbv12I GWGCWC 1 cut(s) 448
BbvI GCAGC 4 cut(s) 239, 358, 361, 478
BccI CCATC 3 cut(s) 338, 416, 508
BciT130I CCWGG 1 cut(s) 484
BcnI CCSGG 4 cut(s) 99, 108, 116, 117
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 1 cut(s) 462
BisI GCNGC 7 cut(s) 121, 159, 162, 228, 372, 375, 492
BlsI GCNGC 7 cut(s) 122, 160, 163, 229, 373, 376, 493
Bme1390I CCNGG 5 cut(s) 99, 108, 116, 117, 484
BmeT110I CYCGRG 1 cut(s) 115
BmgT120I GGNCC 2 cut(s) 57, 62
BmiI GGNNCC 3 cut(s) 58, 80, 112
BmrFI CCNGG 5 cut(s) 99, 108, 116, 117, 484
BmrI ACTGGG 2 cut(s) 23, 136
BmsI GCATC 1 cut(s) 402
BmuI ACTGGG 2 cut(s) 23, 136
BpuMI CCSGG 4 cut(s) 99, 108, 116, 117
BsaJI CCNNGG 5 cut(s) 13, 114, 115, 337, 468
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 54, 88
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 4 cut(s) 29, 142, 238, 531
BseBI CCWGG 1 cut(s) 484
BseDI CCNNGG 5 cut(s) 13, 114, 115, 337, 468
BseLI CCNNNNNNNGG 3 cut(s) 19, 54, 88
BseNI ACTGG 4 cut(s) 29, 142, 238, 531
BseSI GKGCMC 2 cut(s) 433, 448
BseXI GCAGC 4 cut(s) 239, 358, 361, 478
Bsh1236I CGCG 2 cut(s) 164, 209
BshFI GGCC 4 cut(s) 59, 63, 123, 179
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 1 cut(s) 448
BsiHKCI CYCGRG 1 cut(s) 115
BsiSI CCGG 5 cut(s) 60, 76, 98, 108, 116
BslI CCNNNNNNNGG 3 cut(s) 19, 54, 88
BsnI GGCC 4 cut(s) 59, 63, 123, 179
BsoBI CYCGRG 1 cut(s) 115
Bsp1286I GDGCHC 2 cut(s) 433, 448
Bsp143I GATC 2 cut(s) 91, 415
Bsp19I CCATGG 2 cut(s) 13, 468
BspACI CCGC 8 cut(s) 71, 120, 156, 159, 162, 207, 389, 433
BspANI GGCC 4 cut(s) 59, 63, 123, 179
BspFNI CGCG 2 cut(s) 164, 209
BspLI GGNNCC 3 cut(s) 58, 80, 112
BspPI GGATC 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 73
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 4 cut(s) 29, 142, 238, 531
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 5 cut(s) 13, 114, 115, 337, 468
BssMI GATC 2 cut(s) 91, 415
BssT1I CCWWGG 2 cut(s) 13, 468
Bst2UI CCWGG 1 cut(s) 484
Bst4CI ACNGT 1 cut(s) 400
BstC8I GCNNGC 4 cut(s) 61, 104, 391, 433
BstDSI CCRYGG 2 cut(s) 13, 468
BstEII GGTNACC 1 cut(s) 349
BstFNI CGCG 2 cut(s) 164, 209
BstHHI GCGC 2 cut(s) 135, 166
BstKTI GATC 2 cut(s) 94, 418
BstMBI GATC 2 cut(s) 91, 415
BstMWI GCNNNNNNNGC 6 cut(s) 108, 117, 252, 304, 380, 432
BstNI CCWGG 1 cut(s) 484
BstNSI RCATGY 1 cut(s) 106
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 5 cut(s) 97, 106, 114, 115, 482
BstSFI CTRYAG 1 cut(s) 462
BstSLI GKGCMC 2 cut(s) 433, 448
BstUI CGCG 2 cut(s) 164, 209
BstV1I GCAGC 4 cut(s) 239, 358, 361, 478
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuRI GGCC 4 cut(s) 59, 63, 123, 179
BtgI CCRYGG 2 cut(s) 13, 468
BtgZI GCGATG 1 cut(s) 357
BtsI GCAGTG 1 cut(s) 248
BtsIMutI CAGTG 2 cut(s) 248, 524
Cac8I GCNNGC 4 cut(s) 61, 104, 391, 433
CaiI CAGNNNCTG 1 cut(s) 233
CfoI GCGC 2 cut(s) 135, 166
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 57, 62
Cfr9I CCCGGG 1 cut(s) 115
Csp6I GTAC 1 cut(s) 41
CviAII CATG 5 cut(s) 14, 103, 175, 314, 469
CviQI GTAC 1 cut(s) 41
DpnI GATC 2 cut(s) 93, 417
DpnII GATC 2 cut(s) 91, 415
EaeI YGGCCR 1 cut(s) 177
EciI GGCGGA 1 cut(s) 145
Eco130I CCWWGG 2 cut(s) 13, 468
Eco88I CYCGRG 1 cut(s) 115
Eco91I GGTNACC 1 cut(s) 349
EcoO65I GGTNACC 1 cut(s) 349
EcoRII CCWGG 1 cut(s) 482
EcoT14I CCWWGG 2 cut(s) 13, 468
ErhI CCWWGG 2 cut(s) 13, 468
FaeI CATG 5 cut(s) 17, 106, 178, 317, 472
FaiI YATR 7 cut(s) 15, 104, 176, 282, 315, 470, 479
FatI CATG 5 cut(s) 13, 102, 174, 313, 468
FauI CCCGC 3 cut(s) 78, 382, 440
Fnu4HI GCNGC 7 cut(s) 121, 159, 162, 228, 372, 375, 492
FseI GGCCGGCC 1 cut(s) 63
Fsp4HI GCNGC 7 cut(s) 121, 159, 162, 228, 372, 375, 492
FspBI CTAG 1 cut(s) 290
GlaI GCGC 2 cut(s) 134, 165
GluI GCNGC 7 cut(s) 121, 159, 162, 228, 372, 375, 492
HaeIII GGCC 4 cut(s) 59, 63, 123, 179
HapII CCGG 5 cut(s) 60, 76, 98, 108, 116
HhaI GCGC 2 cut(s) 135, 166
Hin1II CATG 5 cut(s) 17, 106, 178, 317, 472
Hin6I GCGC 2 cut(s) 133, 164
HinP1I GCGC 2 cut(s) 133, 164
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 1 cut(s) 310
HpaII CCGG 5 cut(s) 60, 76, 98, 108, 116
HphI GGTGA 4 cut(s) 17, 196, 325, 361
Hpy166II GTNNAC 4 cut(s) 25, 274, 333, 446
Hpy188I TCNGA 2 cut(s) 83, 190
Hpy188III TCNNGA 3 cut(s) 419, 511, 534
Hpy8I GTNNAC 4 cut(s) 25, 274, 333, 446
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 2 cut(s) 82, 296
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 2 cut(s) 6, 276
HpyCH4V TGCA 5 cut(s) 227, 241, 307, 446, 494
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 117, 252, 304, 380, 432
HpySE526I ACGT 2 cut(s) 6, 276
Hsp92II CATG 5 cut(s) 17, 106, 178, 317, 472
HspAI GCGC 2 cut(s) 133, 164
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Kzo9I GATC 2 cut(s) 91, 415
LmnI GCTCC 3 cut(s) 78, 84, 252
Lsp1109I GCAGC 4 cut(s) 239, 358, 361, 478
LweI GCATC 1 cut(s) 402
MaeI CTAG 1 cut(s) 290
MaeII ACGT 2 cut(s) 6, 276
MaeIII GTNAC 3 cut(s) 202, 349, 522
MalI GATC 2 cut(s) 93, 417
MbiI CCGCTC 1 cut(s) 73
MboI GATC 2 cut(s) 91, 415
MboII GAAGA 3 cut(s) 47, 86, 142
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 433, 448
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 2 cut(s) 449, 514
MluNI TGGCCA 1 cut(s) 179
MnlI CCTC 7 cut(s) 46, 134, 146, 196, 208, 347, 364
Mox20I TGGCCA 1 cut(s) 179
MroNI GCCGGC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 179
MslI CAYNNNNRTG 1 cut(s) 535
Msp20I TGGCCA 1 cut(s) 179
MspI CCGG 5 cut(s) 60, 76, 98, 108, 116
MspR9I CCNGG 5 cut(s) 99, 108, 116, 117, 484
MvaI CCWGG 1 cut(s) 484
MvnI CGCG 2 cut(s) 164, 209
MwoI GCNNNNNNNGC 6 cut(s) 108, 117, 252, 304, 380, 432
NaeI GCCGGC 1 cut(s) 61
NciI CCSGG 4 cut(s) 99, 108, 116, 117
NcoI CCATGG 2 cut(s) 13, 468
NdeII GATC 2 cut(s) 91, 415
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 5 cut(s) 17, 106, 178, 317, 472
NlaIV GGNNCC 3 cut(s) 58, 80, 112
NmeAIII GCCGAG 1 cut(s) 318
NmuCI GTSAC 3 cut(s) 202, 349, 522
NspI RCATGY 1 cut(s) 106
PaeI GCATGC 1 cut(s) 106
PdiI GCCGGC 1 cut(s) 61
PfeI GAWTC 1 cut(s) 310
PkrI GCNGC 7 cut(s) 122, 160, 163, 229, 373, 376, 493
Psp6I CCWGG 1 cut(s) 482
PspEI GGTNACC 1 cut(s) 349
PspGI CCWGG 1 cut(s) 482
PspN4I GGNNCC 3 cut(s) 58, 80, 112
PspPI GGNCC 2 cut(s) 57, 62
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 91
RigI GGCCGGCC 1 cut(s) 63
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 535
SatI GCNGC 7 cut(s) 121, 159, 162, 228, 372, 375, 492
Sau3AI GATC 2 cut(s) 91, 415
Sau96I GGNCC 2 cut(s) 57, 62
ScrFI CCNGG 5 cut(s) 99, 108, 116, 117, 484
SduI GDGCHC 2 cut(s) 433, 448
SetI ASST 8 cut(s) 9, 188, 222, 232, 257, 279, 356, 463
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 1 cut(s) 462
SmaI CCCGGG 1 cut(s) 117
SmiMI CAYNNNNRTG 1 cut(s) 535
SphI GCATGC 1 cut(s) 106
Sse9I AATT 2 cut(s) 449, 514
SsiI CCGC 8 cut(s) 71, 120, 156, 159, 162, 207, 389, 433
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 5 cut(s) 97, 106, 114, 115, 482
StyI CCWWGG 2 cut(s) 13, 468
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 2 cut(s) 9, 279
TaqI TCGA 1 cut(s) 535
TasI AATT 2 cut(s) 449, 514
TauI GCSGC 3 cut(s) 123, 161, 164
TfiI GAWTC 1 cut(s) 310
TscAI CASTG 2 cut(s) 248, 531
TseFI GTSAC 3 cut(s) 202, 349, 522
TseI GCWGC 4 cut(s) 227, 371, 374, 491
Tsp45I GTSAC 3 cut(s) 202, 349, 522
TspDTI ATGAA 3 cut(s) 17, 101, 466
TspGWI ACGGA 1 cut(s) 190
TspMI CCCGGG 1 cut(s) 115
TspRI CASTG 2 cut(s) 248, 531
VneI GTGCAC 1 cut(s) 444
XapI RAATTY 1 cut(s) 514
XceI RCATGY 1 cut(s) 106
XmaI CCCGGG 1 cut(s) 115
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.