FvH4_3g32060

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0015903
Physical Location & Seq
Forward (+)
27217634 .. 27219757
2124 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32060.t1

Sequence Viewer

Length: 543 bp
ATGAACGTGAGCCACGGGTCGGTTCACCCAGTTGACGATATCCCCACCACAGACGGCGCCGCTGGCCCAAACCCGCCACGCGTCCGCATGAAGGACCTCCCCGGCATGCCCGGCACCCCCGCCGGCCTCGCCCTGCGCCTCTCCCAGTTCCTCTTCTCCGCCGCCGCTCTCGCCGTCATGGCCTCCACCTCTGACTTCCCTTCCGTCACCGCTTTCTGCTACCTAGTTGCGGCTACAGGGTTGCAGACTCTGTGGAGCTTCTCATTGGCCATTGCTGACGTCTATGCACTTCTAGTCGGGCGGTCCTTGCAGAGCCATAAAGTTGTCTGCTTGTTCACTCTTGGCGATGGGATGACATCCACACTAACATTTGCGGCAGCCTGTGCTTCTGCGGGGATTACAGTTCTTATTGACAATGATCTTGATAGTTGTGCCCATAATCATTGTGCACAATTTGAAACAGCTACAGCCATGGCTTTCATAACCTGGTTCACTGCTCTACCGTCTTTTCTCTTGAATTTTTGGTCGCTGGCATCCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

181

Amino Acids

18.92

Weight (kDa)

5.55

Isoelectric Point (pI)

36.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 164 1.6e-26 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28370
fragaria_vesca FvH4_3g32060
malus_domestica MD03G1119100.v1.1 MD11G1136900.v1.1
prunus_persica Prupe.6G102400_v2.0.a1
pyrus_communis pycom11g11350
rosa_chinensis RchiOBHm_Chr5g0058781
rosa_laevigata RLG00000035248
rosa_multiflora Rmu_sc0008874.1_g000008
rosa_roxburghii Rroxscaffold_1G00021620
rosa_rugosa Rorug05G0319100
rosa_samantha Rh5AG383800 Rh5CG419600 Rh5DG411400
rosa_wichuraiana Rw5G036170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 282
AccB1I GGYRCC 2 cut(s) 56, 113
AccBSI CCGCTC 1 cut(s) 167
AccII CGCG 1 cut(s) 81
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 1 cut(s) 517
AcyI GRCGYC 2 cut(s) 57, 279
AfiI CCNNNNNNNGG 3 cut(s) 19, 91, 229
AflIII ACRYGT 1 cut(s) 79
AgsI TTSAA 2 cut(s) 458, 517
AjnI CCWGG 2 cut(s) 485, 536
AluBI AGCT 2 cut(s) 258, 464
AluI AGCT 2 cut(s) 258, 464
Alw21I GWGCWC 1 cut(s) 451
Alw44I GTGCAC 1 cut(s) 447
AlwNI CAGNNNCTG 1 cut(s) 250
AoxI GGCC 4 cut(s) 64, 124, 180, 267
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 377
ApoI RAATTY 1 cut(s) 517
AspLEI GCGC 2 cut(s) 59, 138
AspS9I GGNCC 3 cut(s) 65, 94, 303
AsuC2I CCSGG 2 cut(s) 102, 111
AsuHPI GGTGA 2 cut(s) 17, 199
AvaII GGWCC 2 cut(s) 94, 303
BaeGI GKGCMC 2 cut(s) 436, 451
BalI TGGCCA 1 cut(s) 269
BanI GGYRCC 2 cut(s) 56, 113
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 389
BccI CCATC 1 cut(s) 341
BceAI ACGGC 2 cut(s) 70, 158
BciT130I CCWGG 2 cut(s) 487, 538
BcnI CCSGG 2 cut(s) 102, 111
BfaI CTAG 2 cut(s) 224, 293
BfmI CTRYAG 2 cut(s) 234, 465
BfoI RGCGCY 1 cut(s) 60
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
BlsI GCNGC 6 cut(s) 61, 163, 166, 232, 376, 379
Bme1390I CCNGG 4 cut(s) 102, 111, 487, 538
Bme18I GGWCC 2 cut(s) 94, 303
BmgT120I GGNCC 3 cut(s) 65, 94, 303
BmiI GGNNCC 2 cut(s) 58, 115
BmrFI CCNGG 4 cut(s) 102, 111, 487, 538
BmrI ACTGGG 2 cut(s) 23, 139
BmuI ACTGGG 2 cut(s) 23, 139
BpuMI CCSGG 2 cut(s) 102, 111
BsaHI GRCGYC 2 cut(s) 57, 279
BsaJI CCNNGG 3 cut(s) 13, 100, 471
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 91, 229
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 2 cut(s) 29, 145
Bse3DI GCAATG 1 cut(s) 270
BseBI CCWGG 2 cut(s) 487, 538
BseDI CCNNGG 3 cut(s) 13, 100, 471
BseGI GGATG 3 cut(s) 356, 357, 533
BseLI CCNNNNNNNGG 3 cut(s) 19, 91, 229
BseMI GCAATG 1 cut(s) 270
BseNI ACTGG 2 cut(s) 29, 145
BseSI GKGCMC 2 cut(s) 436, 451
BseXI GCAGC 1 cut(s) 389
Bsh1236I CGCG 1 cut(s) 81
BshFI GGCC 4 cut(s) 66, 126, 182, 269
BshNI GGYRCC 2 cut(s) 56, 113
BsiHKAI GWGCWC 1 cut(s) 451
BsiSI CCGG 3 cut(s) 102, 111, 123
BslI CCNNNNNNNGG 3 cut(s) 19, 91, 229
BsnI GGCC 4 cut(s) 66, 126, 182, 269
Bsp1286I GDGCHC 2 cut(s) 436, 451
Bsp143I GATC 1 cut(s) 418
Bsp19I CCATGG 1 cut(s) 471
BspANI GGCC 4 cut(s) 66, 126, 182, 269
BspFNI CGCG 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 58, 115
BspT107I GGYRCC 2 cut(s) 56, 113
BsrBI CCGCTC 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 122
BsrI ACTGG 2 cut(s) 29, 145
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 3 cut(s) 13, 100, 471
BssMI GATC 1 cut(s) 418
BssNI GRCGYC 2 cut(s) 57, 279
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 2 cut(s) 487, 538
Bst4CI ACNGT 2 cut(s) 403, 504
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 2 cut(s) 57, 279
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 4 cut(s) 64, 107, 124, 531
BstDSI CCRYGG 2 cut(s) 13, 471
BstF5I GGATG 3 cut(s) 356, 357, 533
BstFNI CGCG 1 cut(s) 81
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 138
BstKTI GATC 1 cut(s) 421
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 7 cut(s) 63, 111, 128, 170, 179, 307, 383
BstNI CCWGG 2 cut(s) 487, 538
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 4 cut(s) 100, 109, 485, 536
BstSFI CTRYAG 2 cut(s) 234, 465
BstSLI GKGCMC 2 cut(s) 436, 451
BstUI CGCG 1 cut(s) 81
BstV1I GCAGC 1 cut(s) 389
BsuRI GGCC 4 cut(s) 66, 126, 182, 269
BtgI CCRYGG 2 cut(s) 13, 471
BtgZI GCGATG 1 cut(s) 360
BtsCI GGATG 3 cut(s) 356, 357, 533
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 1 cut(s) 492
Cac8I GCNNGC 4 cut(s) 64, 107, 124, 531
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 2 cut(s) 59, 138
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 65, 94, 303
CseI GACGC 1 cut(s) 70
CsiI ACCWGGT 1 cut(s) 485
CviAII CATG 4 cut(s) 88, 106, 178, 472
DinI GGCGCC 1 cut(s) 58
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 471
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 94, 303
EcoO109I RGGNCCY 1 cut(s) 94
EcoRII CCWGG 2 cut(s) 485, 536
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 1 cut(s) 471
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 4 cut(s) 91, 109, 181, 475
FaiI YATR 8 cut(s) 89, 107, 179, 285, 318, 438, 473, 482
FatI CATG 4 cut(s) 87, 105, 177, 471
FauI CCCGC 3 cut(s) 81, 127, 385
Fnu4HI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
FokI GGATG 3 cut(s) 343, 364, 520
Fsp4HI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
FspBI CTAG 2 cut(s) 224, 293
GlaI GCGC 2 cut(s) 58, 137
GluI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 4 cut(s) 66, 126, 182, 269
HapII CCGG 3 cut(s) 102, 111, 123
HgaI GACGC 1 cut(s) 70
HhaI GCGC 2 cut(s) 59, 138
Hin1I GRCGYC 2 cut(s) 57, 279
Hin1II CATG 4 cut(s) 91, 109, 181, 475
Hin6I GCGC 2 cut(s) 57, 136
HinP1I GCGC 2 cut(s) 57, 136
HincII GTYRAC 1 cut(s) 34
HindII GTYRAC 1 cut(s) 34
HinfI GANTC 1 cut(s) 247
HpaII CCGG 3 cut(s) 102, 111, 123
HphI GGTGA 2 cut(s) 17, 199
Hpy166II GTNNAC 5 cut(s) 25, 34, 336, 449, 492
Hpy188I TCNGA 1 cut(s) 193
Hpy188III TCNNGA 2 cut(s) 422, 514
Hpy8I GTNNAC 5 cut(s) 25, 34, 336, 449, 492
HpyAV CCTTC 2 cut(s) 85, 210
HpyCH4III ACNGT 2 cut(s) 403, 504
HpyCH4IV ACGT 2 cut(s) 6, 279
HpyCH4V TGCA 4 cut(s) 244, 287, 310, 449
HpyF10VI GCNNNNNNNGC 7 cut(s) 63, 111, 128, 170, 179, 307, 383
HpySE526I ACGT 2 cut(s) 6, 279
Hsp92I GRCGYC 2 cut(s) 57, 279
Hsp92II CATG 4 cut(s) 91, 109, 181, 475
HspAI GCGC 2 cut(s) 57, 136
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 122
KroNI GCCGGC 1 cut(s) 124
Kzo9I GATC 1 cut(s) 418
LmnI GCTCC 1 cut(s) 255
Lsp1109I GCAGC 1 cut(s) 389
MabI ACCWGGT 1 cut(s) 485
MaeI CTAG 2 cut(s) 224, 293
MaeII ACGT 2 cut(s) 6, 279
MaeIII GTNAC 1 cut(s) 205
MalI GATC 1 cut(s) 420
MbiI CCGCTC 1 cut(s) 167
MboI GATC 1 cut(s) 418
MboII GAAGA 1 cut(s) 145
MhlI GDGCHC 2 cut(s) 436, 451
MlsI TGGCCA 1 cut(s) 269
MluCI AATT 2 cut(s) 452, 517
MluI ACGCGT 1 cut(s) 79
MluNI TGGCCA 1 cut(s) 269
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 241
MnlI CCTC 6 cut(s) 107, 137, 149, 161, 193, 199
Mox20I TGGCCA 1 cut(s) 269
MroNI GCCGGC 1 cut(s) 122
MscI TGGCCA 1 cut(s) 269
MslI CAYNNNNRTG 1 cut(s) 538
Msp20I TGGCCA 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 3 cut(s) 102, 111, 123
MspR9I CCNGG 4 cut(s) 102, 111, 487, 538
MvaI CCWGG 2 cut(s) 487, 538
MvnI CGCG 1 cut(s) 81
MwoI GCNNNNNNNGC 7 cut(s) 63, 111, 128, 170, 179, 307, 383
NaeI GCCGGC 1 cut(s) 124
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 2 cut(s) 102, 111
NcoI CCATGG 1 cut(s) 471
NdeII GATC 1 cut(s) 418
NgoMIV GCCGGC 1 cut(s) 122
NlaIII CATG 4 cut(s) 91, 109, 181, 475
NlaIV GGNNCC 2 cut(s) 58, 115
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 1 cut(s) 109
PaeI GCATGC 1 cut(s) 109
PdiI GCCGGC 1 cut(s) 124
PkrI GCNGC 6 cut(s) 61, 163, 166, 232, 376, 379
PleI GAGTC 1 cut(s) 241
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 94
Psp5II RGGWCCY 1 cut(s) 94
Psp6I CCWGG 2 cut(s) 485, 536
PspGI CCWGG 2 cut(s) 485, 536
PspN4I GGNNCC 2 cut(s) 58, 115
PspPI GGNCC 3 cut(s) 65, 94, 303
PspPPI RGGWCCY 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 538
SatI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
Sau3AI GATC 1 cut(s) 418
Sau96I GGNCC 3 cut(s) 65, 94, 303
SchI GAGTC 1 cut(s) 241
ScrFI CCNGG 4 cut(s) 102, 111, 487, 538
SduI GDGCHC 2 cut(s) 436, 451
SetI ASST 9 cut(s) 9, 99, 191, 225, 260, 282, 466, 488, 542
SexAI ACCWGGT 1 cut(s) 485
SfcI CTRYAG 2 cut(s) 234, 465
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 2 cut(s) 94, 303
SmiMI CAYNNNNRTG 1 cut(s) 538
SphI GCATGC 1 cut(s) 109
Sse9I AATT 2 cut(s) 452, 517
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 2 cut(s) 224, 293
StyD4I CCNGG 4 cut(s) 100, 109, 485, 536
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 2 cut(s) 403, 504
TaiI ACGT 2 cut(s) 9, 282
TasI AATT 2 cut(s) 452, 517
TauI GCSGC 5 cut(s) 62, 164, 167, 233, 377
TscAI CASTG 1 cut(s) 499
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 377
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 3 cut(s) 17, 104, 469
TspGWI ACGGA 1 cut(s) 193
TspRI CASTG 1 cut(s) 499
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 94, 303
XapI RAATTY 1 cut(s) 517
XceI RCATGY 1 cut(s) 109
XspI CTAG 2 cut(s) 224, 293
ZraI GACGTC 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.