MD03G1119100.v1.1

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015903
Physical Location & Seq
Reverse (-)
10896334 .. 10898580
2247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1119100.v1.1.491

Sequence Viewer

Length: 540 bp
ATGAACGTGAGCCATGGGTCGGTTCACCCAGTTGAAGATGTACCCACCACAGACGGCGCCGGCCCGAACCCACCCCGGGTCCGAATGAAGGATCTTCCTGGCATGCCCGGCACCCCGGGCGGCCTCGTCTTGCGCCTCGCCCAGTTCTTCTTCTCCGCCGCCTCGCTCGTCGTCATGGCCACCACCTCTGACTTCCCCTCCGTCACTGCGTTTTGCTACCTTGTTGCAGCTACTGGTTTGCAGTGTTTGTGGAGCTTCTCGTTGGGCGTTGTTGACGTTTATGCCCTTCTAGTTGGGCGTTCCTTGCAGAACCATCGAGTTGTGTGTTTGTTCACCCTTGGTGATGGGGTGACATCCACTCTTACATTTGCTGCAGCTTGTGCTTCTGCAGGCATCACAGTTCTTATTGACAATGATCTTAGCAGCTGTGCCCACAACCATTGTGCCCAATTTGAAACAGCTACAGCGATGGCTTTCATATCCTGGTTCGCTGTGTTACCATCTTTTCTCTTGAATTTCTGGTCACTGGCATCTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

180

Amino Acids

18.92

Weight (kDa)

5.85

Isoelectric Point (pI)

35.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 164 2e-28 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28370
fragaria_vesca FvH4_3g32060
malus_domestica MD03G1119100.v1.1 MD11G1136900.v1.1
prunus_persica Prupe.6G102400_v2.0.a1
pyrus_communis pycom11g11350
rosa_chinensis RchiOBHm_Chr5g0058781
rosa_laevigata RLG00000035248
rosa_multiflora Rmu_sc0008874.1_g000008
rosa_roxburghii Rroxscaffold_1G00021620
rosa_rugosa Rorug05G0319100
rosa_samantha Rh5AG383800 Rh5CG419600 Rh5DG411400
rosa_wichuraiana Rw5G036170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 56, 110
AciI CCGC 3 cut(s) 120, 156, 159
AclWI GGATC 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 177
AcsI RAATTY 1 cut(s) 514
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 1 cut(s) 42
AfiI CCNNNNNNNGG 3 cut(s) 19, 76, 88
AgsI TTSAA 3 cut(s) 35, 455, 514
AjnI CCWGG 2 cut(s) 97, 482
AluBI AGCT 5 cut(s) 230, 255, 377, 426, 461
AluI AGCT 5 cut(s) 230, 255, 377, 426, 461
AlwI GGATC 1 cut(s) 99
AlwNI CAGNNNCTG 1 cut(s) 233
Ama87I CYCGRG 2 cut(s) 75, 115
AoxI GGCC 3 cut(s) 61, 121, 177
ApeKI GCWGC 4 cut(s) 227, 371, 374, 423
ApoI RAATTY 1 cut(s) 514
AspLEI GCGC 2 cut(s) 59, 135
AspS9I GGNCC 2 cut(s) 62, 79
AsuC2I CCSGG 5 cut(s) 76, 77, 108, 116, 117
AsuHPI GGTGA 4 cut(s) 17, 325, 353, 361
AvaI CYCGRG 2 cut(s) 75, 115
AvaII GGWCC 1 cut(s) 79
BaeGI GKGCMC 2 cut(s) 433, 448
BalI TGGCCA 1 cut(s) 179
BanI GGYRCC 2 cut(s) 56, 110
BbvI GCAGC 4 cut(s) 239, 358, 386, 435
BccI CCATC 4 cut(s) 321, 338, 463, 508
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 2 cut(s) 296, 330
BciT130I CCWGG 2 cut(s) 99, 484
BcnI CCSGG 5 cut(s) 76, 77, 108, 116, 117
BfaI CTAG 1 cut(s) 290
BfmI CTRYAG 3 cut(s) 372, 387, 462
BfoI RGCGCY 1 cut(s) 60
BisI GCNGC 6 cut(s) 121, 159, 228, 372, 375, 424
BlsI GCNGC 6 cut(s) 122, 160, 229, 373, 376, 425
Bme1390I CCNGG 7 cut(s) 76, 77, 99, 108, 116, 117, 484
Bme18I GGWCC 1 cut(s) 79
BmeT110I CYCGRG 2 cut(s) 75, 115
BmgT120I GGNCC 2 cut(s) 62, 79
BmiI GGNNCC 3 cut(s) 58, 80, 112
BmrFI CCNGG 7 cut(s) 76, 77, 99, 108, 116, 117, 484
BmrI ACTGGG 2 cut(s) 23, 136
BmsI GCATC 1 cut(s) 402
BmuI ACTGGG 2 cut(s) 23, 136
BpuMI CCSGG 5 cut(s) 76, 77, 108, 116, 117
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 6 cut(s) 13, 74, 75, 114, 115, 337
BsaXI ACNNNNNCTCC 2 cut(s) 182, 212
Bsc4I CCNNNNNNNGG 3 cut(s) 19, 76, 88
Bse118I RCCGGY 1 cut(s) 59
Bse1I ACTGG 4 cut(s) 29, 142, 238, 531
BseBI CCWGG 2 cut(s) 99, 484
BseDI CCNNGG 6 cut(s) 13, 74, 75, 114, 115, 337
BseGI GGATG 1 cut(s) 353
BseLI CCNNNNNNNGG 3 cut(s) 19, 76, 88
BseNI ACTGG 4 cut(s) 29, 142, 238, 531
BseSI GKGCMC 2 cut(s) 433, 448
BseXI GCAGC 4 cut(s) 239, 358, 386, 435
BshFI GGCC 3 cut(s) 63, 123, 179
BshNI GGYRCC 2 cut(s) 56, 110
BsiHKCI CYCGRG 2 cut(s) 75, 115
BsiSI CCGG 4 cut(s) 60, 76, 108, 116
BslI CCNNNNNNNGG 3 cut(s) 19, 76, 88
BsnI GGCC 3 cut(s) 63, 123, 179
BsoBI CYCGRG 2 cut(s) 75, 115
Bsp1286I GDGCHC 2 cut(s) 433, 448
Bsp143I GATC 2 cut(s) 91, 415
Bsp19I CCATGG 1 cut(s) 13
BspACI CCGC 3 cut(s) 120, 156, 159
BspANI GGCC 3 cut(s) 63, 123, 179
BspLI GGNNCC 3 cut(s) 58, 80, 112
BspMAI CTGCAG 2 cut(s) 376, 391
BspPI GGATC 1 cut(s) 99
BspT107I GGYRCC 2 cut(s) 56, 110
BsrFI RCCGGY 1 cut(s) 59
BsrI ACTGG 4 cut(s) 29, 142, 238, 531
BssAI RCCGGY 1 cut(s) 59
BssECI CCNNGG 6 cut(s) 13, 74, 75, 114, 115, 337
BssMI GATC 2 cut(s) 91, 415
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 2 cut(s) 13, 337
Bst2UI CCWGG 2 cut(s) 99, 484
Bst4CI ACNGT 1 cut(s) 400
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 380
BstC8I GCNNGC 3 cut(s) 61, 104, 391
BstDEI CTNAG 1 cut(s) 419
BstDSI CCRYGG 1 cut(s) 13
BstF5I GGATG 1 cut(s) 353
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 135
BstKTI GATC 2 cut(s) 94, 418
BstMBI GATC 2 cut(s) 91, 415
BstMWI GCNNNNNNNGC 4 cut(s) 108, 117, 304, 380
BstNI CCWGG 2 cut(s) 99, 484
BstNSI RCATGY 1 cut(s) 106
BstSCI CCNGG 7 cut(s) 74, 75, 97, 106, 114, 115, 482
BstSFI CTRYAG 3 cut(s) 372, 387, 462
BstSLI GKGCMC 2 cut(s) 433, 448
BstV1I GCAGC 4 cut(s) 239, 358, 386, 435
BstX2I RGATCY 1 cut(s) 91
BstYI RGATCY 1 cut(s) 91
BsuRI GGCC 3 cut(s) 63, 123, 179
BtgI CCRYGG 1 cut(s) 13
BtgZI GCGATG 1 cut(s) 482
BtsCI GGATG 1 cut(s) 353
BtsI GCAGTG 2 cut(s) 204, 248
BtsIMutI CAGTG 3 cut(s) 204, 248, 524
Cac8I GCNNGC 3 cut(s) 61, 104, 391
CaiI CAGNNNCTG 1 cut(s) 233
CfoI GCGC 2 cut(s) 59, 135
Cfr10I RCCGGY 1 cut(s) 59
Cfr13I GGNCC 2 cut(s) 62, 79
Cfr9I CCCGGG 2 cut(s) 75, 115
Csp6I GTAC 1 cut(s) 41
CspCI CAANNNNNGTGG 2 cut(s) 422, 457
CviAII CATG 3 cut(s) 14, 103, 175
CviQI GTAC 1 cut(s) 41
DdeI CTNAG 1 cut(s) 419
DinI GGCGCC 1 cut(s) 58
DpnI GATC 2 cut(s) 93, 417
DpnII GATC 2 cut(s) 91, 415
EaeI YGGCCR 1 cut(s) 177
EciI GGCGGA 1 cut(s) 145
Eco130I CCWWGG 2 cut(s) 13, 337
Eco47I GGWCC 1 cut(s) 79
Eco88I CYCGRG 2 cut(s) 75, 115
EcoRII CCWGG 2 cut(s) 97, 482
EcoT14I CCWWGG 2 cut(s) 13, 337
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 2 cut(s) 13, 337
FaeI CATG 3 cut(s) 17, 106, 178
FaiI YATR 5 cut(s) 15, 104, 176, 282, 479
FatI CATG 3 cut(s) 13, 102, 174
Fnu4HI GCNGC 6 cut(s) 121, 159, 228, 372, 375, 424
FokI GGATG 1 cut(s) 340
Fsp4HI GCNGC 6 cut(s) 121, 159, 228, 372, 375, 424
FspBI CTAG 1 cut(s) 290
GlaI GCGC 2 cut(s) 58, 134
GluI GCNGC 6 cut(s) 121, 159, 228, 372, 375, 424
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 3 cut(s) 63, 123, 179
HapII CCGG 4 cut(s) 60, 76, 108, 116
HhaI GCGC 2 cut(s) 59, 135
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 3 cut(s) 17, 106, 178
Hin6I GCGC 2 cut(s) 57, 133
HinP1I GCGC 2 cut(s) 57, 133
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HpaII CCGG 4 cut(s) 60, 76, 108, 116
HphI GGTGA 4 cut(s) 17, 325, 353, 361
Hpy166II GTNNAC 3 cut(s) 25, 274, 333
Hpy188I TCNGA 2 cut(s) 83, 190
Hpy188III TCNNGA 2 cut(s) 511, 534
Hpy8I GTNNAC 3 cut(s) 25, 274, 333
Hpy99I CGWCG 1 cut(s) 173
HpyAV CCTTC 2 cut(s) 82, 296
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 2 cut(s) 6, 276
HpyCH4V TGCA 5 cut(s) 227, 241, 307, 374, 389
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 117, 304, 380
HpyF3I CTNAG 1 cut(s) 419
HpySE526I ACGT 2 cut(s) 6, 276
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 3 cut(s) 17, 106, 178
HspAI GCGC 2 cut(s) 57, 133
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 59
KroNI GCCGGC 1 cut(s) 61
Kzo9I GATC 2 cut(s) 91, 415
LmnI GCTCC 1 cut(s) 252
Lsp1109I GCAGC 4 cut(s) 239, 358, 386, 435
LweI GCATC 1 cut(s) 402
MaeI CTAG 1 cut(s) 290
MaeII ACGT 2 cut(s) 6, 276
MaeIII GTNAC 4 cut(s) 202, 349, 495, 522
MalI GATC 2 cut(s) 93, 417
MboI GATC 2 cut(s) 91, 415
MboII GAAGA 4 cut(s) 47, 86, 139, 142
MflI RGATCY 1 cut(s) 91
MhlI GDGCHC 2 cut(s) 433, 448
MlsI TGGCCA 1 cut(s) 179
MluCI AATT 2 cut(s) 449, 514
MluNI TGGCCA 1 cut(s) 179
Mly113I GGCGCC 1 cut(s) 57
MnlI CCTC 5 cut(s) 134, 146, 172, 196, 208
Mox20I TGGCCA 1 cut(s) 179
MroNI GCCGGC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 179
MslI CAYNNNNRTG 1 cut(s) 535
Msp20I TGGCCA 1 cut(s) 179
MspA1I CMGCKG 1 cut(s) 426
MspI CCGG 4 cut(s) 60, 76, 108, 116
MspR9I CCNGG 7 cut(s) 76, 77, 99, 108, 116, 117, 484
MvaI CCWGG 2 cut(s) 99, 484
MwoI GCNNNNNNNGC 4 cut(s) 108, 117, 304, 380
NaeI GCCGGC 1 cut(s) 61
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 5 cut(s) 76, 77, 108, 116, 117
NcoI CCATGG 1 cut(s) 13
NdeII GATC 2 cut(s) 91, 415
NgoMIV GCCGGC 1 cut(s) 59
NlaIII CATG 3 cut(s) 17, 106, 178
NlaIV GGNNCC 3 cut(s) 58, 80, 112
NmuCI GTSAC 3 cut(s) 202, 349, 522
NspI RCATGY 1 cut(s) 106
PaeI GCATGC 1 cut(s) 106
PdiI GCCGGC 1 cut(s) 61
PkrI GCNGC 6 cut(s) 122, 160, 229, 373, 376, 425
PluTI GGCGCC 1 cut(s) 60
Psp6I CCWGG 2 cut(s) 97, 482
PspGI CCWGG 2 cut(s) 97, 482
PspN4I GGNNCC 3 cut(s) 58, 80, 112
PspPI GGNCC 2 cut(s) 62, 79
PstI CTGCAG 2 cut(s) 376, 391
PstNI CAGNNNCTG 1 cut(s) 233
PsuI RGATCY 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 426
RsaI GTAC 1 cut(s) 42
RsaNI GTAC 1 cut(s) 41
RseI CAYNNNNRTG 1 cut(s) 535
SatI GCNGC 6 cut(s) 121, 159, 228, 372, 375, 424
Sau3AI GATC 2 cut(s) 91, 415
Sau96I GGNCC 2 cut(s) 62, 79
ScrFI CCNGG 7 cut(s) 76, 77, 99, 108, 116, 117, 484
SduI GDGCHC 2 cut(s) 433, 448
SetI ASST 9 cut(s) 9, 188, 222, 232, 257, 279, 379, 428, 463
SfaNI GCATC 1 cut(s) 402
SfcI CTRYAG 3 cut(s) 372, 387, 462
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 1 cut(s) 79
SmaI CCCGGG 2 cut(s) 77, 117
SmiMI CAYNNNNRTG 1 cut(s) 535
SphI GCATGC 1 cut(s) 106
Sse9I AATT 2 cut(s) 449, 514
SsiI CCGC 3 cut(s) 120, 156, 159
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 1 cut(s) 290
StyD4I CCNGG 7 cut(s) 74, 75, 97, 106, 114, 115, 482
StyI CCWWGG 2 cut(s) 13, 337
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 2 cut(s) 9, 279
TaqI TCGA 2 cut(s) 316, 535
TasI AATT 2 cut(s) 449, 514
TauI GCSGC 2 cut(s) 123, 161
TscAI CASTG 3 cut(s) 211, 248, 531
TseFI GTSAC 3 cut(s) 202, 349, 522
TseI GCWGC 4 cut(s) 227, 371, 374, 423
Tsp45I GTSAC 3 cut(s) 202, 349, 522
TspDTI ATGAA 3 cut(s) 17, 101, 466
TspGWI ACGGA 1 cut(s) 190
TspMI CCCGGG 2 cut(s) 75, 115
TspRI CASTG 3 cut(s) 211, 248, 531
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 1 cut(s) 514
XceI RCATGY 1 cut(s) 106
XmaI CCCGGG 2 cut(s) 75, 115
XspI CTAG 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.