Rroxscaffold_1G00021620

Belongs to the Casparian strip membrane proteins (CASP) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0015903
Physical Location & Seq
Reverse (-)
26577385 .. 26579396
2012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00021620.1

Sequence Viewer

Length: 543 bp
ATGAATGTGAGCCACGGGTCGGTTCACCCAGTTGAAGATGTCCCCACCACAGACGGCGCCGCCGGCCCAAACCCGCCCCGGGTCCGAATGAAGGACCTCCCCGGCATGCCCGGCACTCCCGCCGGCCTCGCCTTGCGCCTTTCCCAGTTCCTCTTCTCCGCCGCCGCTCTCGCCGTCATGGCCTCCACCTCTGACTTCCCTTCCGTCACCGCTTTCTGCTACCTAGTTGCCGCTACCGGGTTGCAGACTCTGTGGAGCTTCTCACTGGCCGTTGCTGACGTTTATGCCCTTCTAGTGGGGCGTTCTTTGCAGAGCCATAAAGTTGTCTGCTTGTTCACTTTCGGCGATGGGATGACATCCACTCTTACTTTCGCGGCAGCTTGTGCTTCTGCGGGGATCACAGTTCTTATTGACAATGATCTTGATAGTTGTGCCCATAACCATTGTGCACAATTTGAAACAGCTACAGCCATGGCTTTTATAACCTGGTTCACTGCACTACCATCTTTTCTTTTAAACTTTTGGTCGTTGGCATCCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF588

Protein Analysis

180

Amino Acids

18.94

Weight (kDa)

5.56

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CASP_dom PF04535 37 - 164 1.7e-26 Casparian strip membrane protein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015903)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G28370
fragaria_vesca FvH4_3g32060
malus_domestica MD03G1119100.v1.1 MD11G1136900.v1.1
prunus_persica Prupe.6G102400_v2.0.a1
pyrus_communis pycom11g11350
rosa_chinensis RchiOBHm_Chr5g0058781
rosa_laevigata RLG00000035248
rosa_multiflora Rmu_sc0008874.1_g000008
rosa_roxburghii Rroxscaffold_1G00021620
rosa_rugosa Rorug05G0319100
rosa_samantha Rh5AG383800 Rh5CG419600 Rh5DG411400
rosa_wichuraiana Rw5G036170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 167
AccII CGCG 1 cut(s) 374
AclWI GGATC 1 cut(s) 404
AcoI YGGCCR 1 cut(s) 267
AcyI GRCGYC 1 cut(s) 57
AfiI CCNNNNNNNGG 5 cut(s) 19, 79, 91, 237, 295
AgsI TTSAA 2 cut(s) 35, 458
AjnI CCWGG 1 cut(s) 485
AluBI AGCT 3 cut(s) 258, 380, 464
AluI AGCT 3 cut(s) 258, 380, 464
Alw21I GWGCWC 1 cut(s) 451
Alw44I GTGCAC 1 cut(s) 447
AlwI GGATC 1 cut(s) 404
AlwNI CAGNNNCTG 1 cut(s) 250
Ama87I CYCGRG 1 cut(s) 78
AoxI GGCC 4 cut(s) 64, 124, 180, 267
ApaLI GTGCAC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 377
AspLEI GCGC 2 cut(s) 59, 138
AspS9I GGNCC 3 cut(s) 65, 82, 94
AsuC2I CCSGG 5 cut(s) 79, 80, 102, 111, 238
AsuHPI GGTGA 2 cut(s) 17, 199
AvaI CYCGRG 1 cut(s) 78
AvaII GGWCC 2 cut(s) 82, 94
BaeGI GKGCMC 2 cut(s) 436, 451
BanI GGYRCC 1 cut(s) 56
Bbv12I GWGCWC 1 cut(s) 451
BbvI GCAGC 1 cut(s) 389
BccI CCATC 2 cut(s) 341, 511
BceAI ACGGC 3 cut(s) 70, 158, 254
BciT130I CCWGG 1 cut(s) 487
BcnI CCSGG 5 cut(s) 79, 80, 102, 111, 238
BfaI CTAG 2 cut(s) 224, 293
BfmI CTRYAG 1 cut(s) 465
BfoI RGCGCY 1 cut(s) 60
BglI GCCNNNNNGGC 1 cut(s) 179
BisI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
BlsI GCNGC 6 cut(s) 61, 163, 166, 232, 376, 379
Bme1390I CCNGG 6 cut(s) 79, 80, 102, 111, 238, 487
Bme18I GGWCC 2 cut(s) 82, 94
BmeT110I CYCGRG 1 cut(s) 78
BmgT120I GGNCC 3 cut(s) 65, 82, 94
BmiI GGNNCC 2 cut(s) 58, 83
BmrFI CCNGG 6 cut(s) 79, 80, 102, 111, 238, 487
BmrI ACTGGG 2 cut(s) 23, 139
BmuI ACTGGG 2 cut(s) 23, 139
BpuMI CCSGG 5 cut(s) 79, 80, 102, 111, 238
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 5 cut(s) 13, 77, 78, 100, 471
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 79, 91, 237, 295
Bse118I RCCGGY 2 cut(s) 62, 122
Bse1I ACTGG 3 cut(s) 29, 145, 270
BseBI CCWGG 1 cut(s) 487
BseDI CCNNGG 5 cut(s) 13, 77, 78, 100, 471
BseGI GGATG 3 cut(s) 356, 357, 533
BseLI CCNNNNNNNGG 5 cut(s) 19, 79, 91, 237, 295
BseNI ACTGG 3 cut(s) 29, 145, 270
BseSI GKGCMC 2 cut(s) 436, 451
BseXI GCAGC 1 cut(s) 389
BsgI GTGCAG 1 cut(s) 480
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 4 cut(s) 66, 126, 182, 269
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 451
BsiHKCI CYCGRG 1 cut(s) 78
BsiSI CCGG 6 cut(s) 63, 79, 102, 111, 123, 237
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 5 cut(s) 19, 79, 91, 237, 295
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 4 cut(s) 66, 126, 182, 269
BsoBI CYCGRG 1 cut(s) 78
Bsp1286I GDGCHC 2 cut(s) 436, 451
Bsp143I GATC 2 cut(s) 396, 418
Bsp19I CCATGG 1 cut(s) 471
BspANI GGCC 4 cut(s) 66, 126, 182, 269
BspFNI CGCG 1 cut(s) 374
BspLI GGNNCC 2 cut(s) 58, 83
BspPI GGATC 1 cut(s) 404
BspT107I GGYRCC 1 cut(s) 56
BsrBI CCGCTC 1 cut(s) 167
BsrFI RCCGGY 2 cut(s) 62, 122
BsrI ACTGG 3 cut(s) 29, 145, 270
BssAI RCCGGY 2 cut(s) 62, 122
BssECI CCNNGG 5 cut(s) 13, 77, 78, 100, 471
BssMI GATC 2 cut(s) 396, 418
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 1 cut(s) 471
Bst2UI CCWGG 1 cut(s) 487
Bst4CI ACNGT 1 cut(s) 403
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 57
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 64, 107, 124
BstDSI CCRYGG 2 cut(s) 13, 471
BstF5I GGATG 3 cut(s) 356, 357, 533
BstFNI CGCG 1 cut(s) 374
BstH2I RGCGCY 1 cut(s) 60
BstHHI GCGC 2 cut(s) 59, 138
BstKTI GATC 2 cut(s) 399, 421
BstMBI GATC 2 cut(s) 396, 418
BstMWI GCNNNNNNNGC 7 cut(s) 63, 111, 128, 170, 179, 307, 383
BstNI CCWGG 1 cut(s) 487
BstNSI RCATGY 1 cut(s) 109
BstSCI CCNGG 6 cut(s) 77, 78, 100, 109, 236, 485
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 2 cut(s) 436, 451
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 389
BsuRI GGCC 4 cut(s) 66, 126, 182, 269
BtgI CCRYGG 2 cut(s) 13, 471
BtgZI GCGATG 1 cut(s) 360
BtsCI GGATG 3 cut(s) 356, 357, 533
BtsI GCAGTG 1 cut(s) 492
BtsIMutI CAGTG 2 cut(s) 263, 492
Cac8I GCNNGC 3 cut(s) 64, 107, 124
CaiI CAGNNNCTG 1 cut(s) 250
CfoI GCGC 2 cut(s) 59, 138
Cfr10I RCCGGY 2 cut(s) 62, 122
Cfr13I GGNCC 3 cut(s) 65, 82, 94
Cfr9I CCCGGG 1 cut(s) 78
CsiI ACCWGGT 1 cut(s) 485
CviAII CATG 3 cut(s) 106, 178, 472
DinI GGCGCC 1 cut(s) 58
DpnI GATC 2 cut(s) 398, 420
DpnII GATC 2 cut(s) 396, 418
DraI TTTAAA 1 cut(s) 516
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 471
Eco47I GGWCC 2 cut(s) 82, 94
Eco88I CYCGRG 1 cut(s) 78
EcoO109I RGGNCCY 1 cut(s) 94
EcoRII CCWGG 1 cut(s) 485
EcoT14I CCWWGG 1 cut(s) 471
EgeI GGCGCC 1 cut(s) 58
EheI GGCGCC 1 cut(s) 58
ErhI CCWWGG 1 cut(s) 471
FaeI CATG 3 cut(s) 109, 181, 475
FaiI YATR 7 cut(s) 107, 179, 285, 318, 438, 473, 482
FaqI GGGAC 1 cut(s) 26
FatI CATG 3 cut(s) 105, 177, 471
FauI CCCGC 3 cut(s) 81, 127, 385
Fnu4HI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
FokI GGATG 3 cut(s) 343, 364, 520
Fsp4HI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
FspBI CTAG 2 cut(s) 224, 293
GlaI GCGC 2 cut(s) 58, 137
GluI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
HaeII RGCGCY 1 cut(s) 60
HaeIII GGCC 4 cut(s) 66, 126, 182, 269
HapII CCGG 6 cut(s) 63, 79, 102, 111, 123, 237
HhaI GCGC 2 cut(s) 59, 138
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 3 cut(s) 109, 181, 475
Hin6I GCGC 2 cut(s) 57, 136
HinP1I GCGC 2 cut(s) 57, 136
HinfI GANTC 1 cut(s) 247
HpaII CCGG 6 cut(s) 63, 79, 102, 111, 123, 237
HphI GGTGA 2 cut(s) 17, 199
Hpy166II GTNNAC 4 cut(s) 25, 336, 449, 492
Hpy188I TCNGA 2 cut(s) 86, 193
Hpy188III TCNNGA 2 cut(s) 422, 537
Hpy8I GTNNAC 4 cut(s) 25, 336, 449, 492
HpyAV CCTTC 3 cut(s) 85, 210, 299
HpyCH4III ACNGT 1 cut(s) 403
HpyCH4IV ACGT 1 cut(s) 279
HpyCH4V TGCA 4 cut(s) 244, 310, 449, 497
HpyF10VI GCNNNNNNNGC 7 cut(s) 63, 111, 128, 170, 179, 307, 383
HpySE526I ACGT 1 cut(s) 279
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 3 cut(s) 109, 181, 475
HspAI GCGC 2 cut(s) 57, 136
KasI GGCGCC 1 cut(s) 56
KroI GCCGGC 2 cut(s) 62, 122
KroNI GCCGGC 2 cut(s) 64, 124
Kzo9I GATC 2 cut(s) 396, 418
LmnI GCTCC 1 cut(s) 255
Lsp1109I GCAGC 1 cut(s) 389
MabI ACCWGGT 1 cut(s) 485
MaeI CTAG 2 cut(s) 224, 293
MaeII ACGT 1 cut(s) 279
MaeIII GTNAC 1 cut(s) 205
MalI GATC 2 cut(s) 398, 420
MbiI CCGCTC 1 cut(s) 167
MboI GATC 2 cut(s) 396, 418
MboII GAAGA 2 cut(s) 47, 145
MhlI GDGCHC 2 cut(s) 436, 451
MluCI AATT 1 cut(s) 452
Mly113I GGCGCC 1 cut(s) 57
MlyI GAGTC 1 cut(s) 241
MnlI CCTC 5 cut(s) 107, 137, 161, 193, 199
MroNI GCCGGC 2 cut(s) 62, 122
MseI TTAA 1 cut(s) 515
MslI CAYNNNNRTG 1 cut(s) 538
MspI CCGG 6 cut(s) 63, 79, 102, 111, 123, 237
MspR9I CCNGG 6 cut(s) 79, 80, 102, 111, 238, 487
MvaI CCWGG 1 cut(s) 487
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 7 cut(s) 63, 111, 128, 170, 179, 307, 383
NaeI GCCGGC 2 cut(s) 64, 124
NarI GGCGCC 1 cut(s) 57
NciI CCSGG 5 cut(s) 79, 80, 102, 111, 238
NcoI CCATGG 1 cut(s) 471
NdeII GATC 2 cut(s) 396, 418
NgoMIV GCCGGC 2 cut(s) 62, 122
NlaIII CATG 3 cut(s) 109, 181, 475
NlaIV GGNNCC 2 cut(s) 58, 83
NmuCI GTSAC 1 cut(s) 205
NspI RCATGY 1 cut(s) 109
PaeI GCATGC 1 cut(s) 109
PdiI GCCGGC 2 cut(s) 64, 124
PkrI GCNGC 6 cut(s) 61, 163, 166, 232, 376, 379
PleI GAGTC 1 cut(s) 241
PluTI GGCGCC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 94
PsiI TTATAA 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 94
Psp6I CCWGG 1 cut(s) 485
PspGI CCWGG 1 cut(s) 485
PspN4I GGNNCC 2 cut(s) 58, 83
PspPI GGNCC 3 cut(s) 65, 82, 94
PspPPI RGGWCCY 1 cut(s) 94
PstNI CAGNNNCTG 1 cut(s) 250
RseI CAYNNNNRTG 1 cut(s) 538
SaqAI TTAA 1 cut(s) 515
SatI GCNGC 6 cut(s) 60, 162, 165, 231, 375, 378
Sau3AI GATC 2 cut(s) 396, 418
Sau96I GGNCC 3 cut(s) 65, 82, 94
SchI GAGTC 1 cut(s) 241
ScrFI CCNGG 6 cut(s) 79, 80, 102, 111, 238, 487
SduI GDGCHC 2 cut(s) 436, 451
SetI ASST 8 cut(s) 99, 191, 225, 260, 282, 382, 466, 488
SexAI ACCWGGT 1 cut(s) 485
SfcI CTRYAG 1 cut(s) 465
SfoI GGCGCC 1 cut(s) 58
SinI GGWCC 2 cut(s) 82, 94
SmaI CCCGGG 1 cut(s) 80
SmiMI CAYNNNNRTG 1 cut(s) 538
SphI GCATGC 1 cut(s) 109
Sse9I AATT 1 cut(s) 452
SspDI GGCGCC 1 cut(s) 56
SspMI CTAG 2 cut(s) 224, 293
StyD4I CCNGG 6 cut(s) 77, 78, 100, 109, 236, 485
StyI CCWWGG 1 cut(s) 471
TaaI ACNGT 1 cut(s) 403
TaiI ACGT 1 cut(s) 282
TasI AATT 1 cut(s) 452
TauI GCSGC 5 cut(s) 62, 164, 167, 233, 377
Tru1I TTAA 1 cut(s) 515
Tru9I TTAA 1 cut(s) 515
TscAI CASTG 2 cut(s) 270, 499
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 1 cut(s) 377
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 2 cut(s) 17, 104
TspGWI ACGGA 1 cut(s) 193
TspMI CCCGGG 1 cut(s) 78
TspRI CASTG 2 cut(s) 270, 499
VneI GTGCAC 1 cut(s) 447
VpaK11BI GGWCC 2 cut(s) 82, 94
XceI RCATGY 1 cut(s) 109
XmaI CCCGGG 1 cut(s) 78
XspI CTAG 2 cut(s) 224, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.