AT2G30230

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014200
Physical Location & Seq
Forward (+)
12897054 .. 12898172
1119 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G30230.1

Sequence Viewer

Length: 534 bp
ATGGGAAACAGTTTACGGTGTTGTTTGGCTTGTGTACTTCCATGTGGAGCTTTAGATTTGATCCGTATCGTACATCTCAACGGCCACGTCGACGAGATCACTAGTCCAATGACCGCCGGCGAGATTCTTCAGGCGAATCCTAACCATGTCTTAAGCAAACCTTGTTCTCAAGGAGTTGTGCGTAAGATCTTGATCTTGTCTCCTGAATCTGAGCTTAAGCGTGGAAGCATCTATTTTCTTATCCCGGATACTTCTATGCCGGAGAAGAAGAAAACAAAGAAGAGAAAAGAGCTCCGATGCCGGAAAAAGCCTCTTGAAAGCGGCAACGATATTAATAGTGATCATGTGAGTACTAATAAGGATCTTGATGGTCATTGTTTGACGTTGTGTGAGAAATATTTGGAAGATGTTATGTTGTCGGAGAAGATGAGTTCGGCCGGTAAAGAGAATCGTCACCGTCGGAAACATAGCCGGTCGGCGTCAGTGTCCACGTGGCGGCCTCACCTTGATAGCATCACTGAGGATCTTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

177

Amino Acids

19.84

Weight (kDa)

8.92

Isoelectric Point (pI)

51.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 174 2e-37 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06980 AT2G30230
fragaria_vesca FvH4_3g39820
malus_domestica MD03G1057000.v1.1 MD11G1058700.v1.1
prunus_persica Prupe.6G047800_v2.0.a1
pyrus_communis pycom03g04530 pycom11g04880
rosa_chinensis RchiOBHm_Chr5g0071711
rosa_laevigata RLG00000036243
rosa_multiflora Rmu_sc0022933.1_g000001
rosa_roxburghii Rroxscaffold_1G00009470
rosa_rugosa Rorug05G0414300
rosa_samantha Rh5BG490000 Rh5CG514000 Rh5DG500800
rosa_wichuraiana Rw5G043710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 90
AciI CCGC 3 cut(s) 114, 321, 496
AclWI GGATC 3 cut(s) 55, 369, 531
AcoI YGGCCR 2 cut(s) 82, 435
AcuI CTGAAG 1 cut(s) 113
AcvI CACGTG 1 cut(s) 492
AcyI GRCGYC 1 cut(s) 479
AfaI GTAC 3 cut(s) 36, 72, 352
AfiI CCNNNNNNNGG 1 cut(s) 495
AflII CTTAAG 2 cut(s) 151, 215
AgsI TTSAA 1 cut(s) 317
AhlI ACTAGT 1 cut(s) 101
AjiI CACGTC 1 cut(s) 88
AluBI AGCT 3 cut(s) 50, 214, 292
AluI AGCT 3 cut(s) 50, 214, 292
Alw21I GWGCWC 1 cut(s) 294
Alw26I GTCTC 1 cut(s) 204
AlwI GGATC 3 cut(s) 55, 369, 531
AoxI GGCC 3 cut(s) 82, 435, 497
AseI ATTAAT 1 cut(s) 333
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 2 cut(s) 446, 494
BanII GRGCYC 1 cut(s) 294
BbrPI CACGTG 1 cut(s) 492
Bbv12I GWGCWC 1 cut(s) 294
BccI CCATC 1 cut(s) 362
BceAI ACGGC 1 cut(s) 97
BciVI GTATCC 1 cut(s) 241
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 1 cut(s) 245
BcoDI GTCTC 1 cut(s) 204
BcuI ACTAGT 1 cut(s) 101
BfaI CTAG 1 cut(s) 102
BfrI CTTAAG 2 cut(s) 151, 215
BfuI GTATCC 1 cut(s) 241
BglII AGATCT 1 cut(s) 186
BisI GCNGC 2 cut(s) 322, 497
BlsI GCNGC 2 cut(s) 323, 498
BmcAI AGTACT 1 cut(s) 352
Bme1390I CCNGG 1 cut(s) 245
BmgBI CACGTC 1 cut(s) 88
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 3 cut(s) 237, 287, 522
BpuEI CTTGAG 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 245
BsaAI YACGTR 1 cut(s) 492
BsaBI GATNNNNATC 2 cut(s) 65, 191
BsaHI GRCGYC 1 cut(s) 479
Bsc4I CCNNNNNNNGG 1 cut(s) 495
Bse118I RCCGGY 3 cut(s) 116, 437, 471
Bse8I GATNNNNATC 2 cut(s) 65, 191
BseJI GATNNNNATC 2 cut(s) 65, 191
BseLI CCNNNNNNNGG 1 cut(s) 495
BseMII CTCAG 2 cut(s) 201, 510
BseX3I CGGCCG 1 cut(s) 435
Bsh1285I CGRYCG 2 cut(s) 438, 476
BshFI GGCC 3 cut(s) 84, 437, 499
BsiEI CGRYCG 2 cut(s) 438, 476
BsiHKAI GWGCWC 1 cut(s) 294
BsiSI CCGG 6 cut(s) 117, 245, 260, 301, 438, 472
BslI CCNNNNNNNGG 1 cut(s) 495
BsmAI GTCTC 1 cut(s) 204
BsnI GGCC 3 cut(s) 84, 437, 499
Bsp1286I GDGCHC 1 cut(s) 294
Bsp143I GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
BspACI CCGC 3 cut(s) 114, 321, 496
BspANI GGCC 3 cut(s) 84, 437, 499
BspCNI CTCAG 2 cut(s) 202, 511
BspPI GGATC 3 cut(s) 55, 369, 531
BspTI CTTAAG 2 cut(s) 151, 215
BsrFI RCCGGY 3 cut(s) 116, 437, 471
BssAI RCCGGY 3 cut(s) 116, 437, 471
BssMI GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
BssNI GRCGYC 1 cut(s) 479
Bst4CI ACNGT 3 cut(s) 11, 18, 458
Bst6I CTCTTC 1 cut(s) 275
BstACI GRCGYC 1 cut(s) 479
BstAFI CTTAAG 2 cut(s) 151, 215
BstBAI YACGTR 1 cut(s) 492
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 2 cut(s) 210, 519
BstKTI GATC 7 cut(s) 63, 99, 189, 195, 343, 364, 526
BstMAI GTCTC 1 cut(s) 204
BstMBI GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
BstMCI CGRYCG 2 cut(s) 438, 476
BstSCI CCNGG 1 cut(s) 243
BstX2I RGATCY 3 cut(s) 186, 361, 523
BstYI RGATCY 3 cut(s) 186, 361, 523
BstZI CGGCCG 1 cut(s) 435
BsuI GTATCC 1 cut(s) 241
BsuRI GGCC 3 cut(s) 84, 437, 499
BtrI CACGTC 1 cut(s) 88
BtsIMutI CAGTG 2 cut(s) 489, 516
Cac8I GCNNGC 1 cut(s) 118
Cfr10I RCCGGY 3 cut(s) 116, 437, 471
CseI GACGC 1 cut(s) 468
Csp6I GTAC 3 cut(s) 35, 71, 351
CviAII CATG 3 cut(s) 42, 146, 344
CviJI RGCY 9 cut(s) 29, 50, 84, 214, 292, 310, 437, 471, 499
CviKI_1 RGCY 9 cut(s) 29, 50, 84, 214, 292, 310, 437, 471, 499
CviQI GTAC 3 cut(s) 35, 71, 351
DdeI CTNAG 2 cut(s) 210, 519
DpnI GATC 7 cut(s) 62, 98, 188, 194, 342, 363, 525
DpnII GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
EaeI YGGCCR 2 cut(s) 82, 435
EagI CGGCCG 1 cut(s) 435
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
Ecl136II GAGCTC 1 cut(s) 292
EclXI CGGCCG 1 cut(s) 435
Eco24I GRGCYC 1 cut(s) 294
Eco52I CGGCCG 1 cut(s) 435
Eco53kI GAGCTC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 113
Eco72I CACGTG 1 cut(s) 492
EcoICRI GAGCTC 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 294
FaeI CATG 3 cut(s) 45, 149, 347
FaiI YATR 6 cut(s) 43, 147, 257, 345, 413, 468
FalI AAGNNNNNCTT 2 cut(s) 145, 177
FatI CATG 3 cut(s) 41, 145, 343
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 90
Fnu4HI GCNGC 2 cut(s) 322, 497
FriOI GRGCYC 1 cut(s) 294
Fsp4HI GCNGC 2 cut(s) 322, 497
FspBI CTAG 1 cut(s) 102
GluI GCNGC 2 cut(s) 322, 497
HaeIII GGCC 3 cut(s) 84, 437, 499
HapII CCGG 6 cut(s) 117, 245, 260, 301, 438, 472
HgaI GACGC 1 cut(s) 468
Hin1I GRCGYC 1 cut(s) 479
Hin1II CATG 3 cut(s) 45, 149, 347
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 4 cut(s) 124, 136, 206, 448
HpaII CCGG 6 cut(s) 117, 245, 260, 301, 438, 472
HphI GGTGA 2 cut(s) 446, 494
Hpy166II GTNNAC 4 cut(s) 14, 35, 91, 489
Hpy188I TCNGA 4 cut(s) 211, 296, 421, 462
Hpy188III TCNNGA 4 cut(s) 190, 203, 314, 365
Hpy8I GTNNAC 4 cut(s) 14, 35, 91, 489
Hpy99I CGWCG 3 cut(s) 92, 95, 462
HpyCH4III ACNGT 3 cut(s) 11, 18, 458
HpyCH4IV ACGT 3 cut(s) 87, 383, 491
HpyF3I CTNAG 2 cut(s) 210, 519
HpySE526I ACGT 3 cut(s) 87, 383, 491
Hsp92I GRCGYC 1 cut(s) 479
Hsp92II CATG 3 cut(s) 45, 149, 347
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
LmnI GCTCC 2 cut(s) 47, 297
LpnPI CCDG 8 cut(s) 116, 130, 216, 258, 273, 314, 451, 485
LweI GCATC 3 cut(s) 237, 287, 522
MaeI CTAG 1 cut(s) 102
MaeII ACGT 3 cut(s) 87, 383, 491
MaeIII GTNAC 1 cut(s) 452
MalI GATC 7 cut(s) 62, 98, 188, 194, 342, 363, 525
MboI GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
MboII GAAGA 6 cut(s) 119, 277, 280, 292, 416, 436
MflI RGATCY 3 cut(s) 186, 361, 523
MhlI GDGCHC 1 cut(s) 294
MluCI AATT 1 cut(s) 529
MmeI TCCRAC 2 cut(s) 399, 440
MnlI CCTC 3 cut(s) 321, 510, 514
MreI CGCCGGCG 1 cut(s) 116
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 5 cut(s) 152, 216, 333, 528, 532
MspCI CTTAAG 2 cut(s) 151, 215
MspI CCGG 6 cut(s) 117, 245, 260, 301, 438, 472
MspR9I CCNGG 1 cut(s) 245
NaeI GCCGGC 1 cut(s) 118
NciI CCSGG 1 cut(s) 245
NdeII GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 3 cut(s) 45, 149, 347
NmuCI GTSAC 1 cut(s) 452
PacI TTAATTAA 1 cut(s) 532
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 4 cut(s) 124, 136, 206, 448
PfoI TCCNGGA 1 cut(s) 243
PkrI GCNGC 2 cut(s) 323, 498
PmaCI CACGTG 1 cut(s) 492
PmlI CACGTG 1 cut(s) 492
Ppu21I YACGTR 1 cut(s) 492
PshBI ATTAAT 1 cut(s) 333
Psp124BI GAGCTC 1 cut(s) 294
PspCI CACGTG 1 cut(s) 492
PsuI RGATCY 3 cut(s) 186, 361, 523
RsaI GTAC 3 cut(s) 36, 72, 352
RsaNI GTAC 3 cut(s) 35, 71, 351
SacI GAGCTC 1 cut(s) 294
SalI GTCGAC 1 cut(s) 89
SaqAI TTAA 5 cut(s) 152, 216, 333, 528, 532
SatI GCNGC 2 cut(s) 322, 497
Sau3AI GATC 7 cut(s) 60, 96, 186, 192, 340, 361, 523
ScaI AGTACT 1 cut(s) 352
ScrFI CCNGG 1 cut(s) 245
SduI GDGCHC 1 cut(s) 294
SetI ASST 8 cut(s) 52, 90, 163, 216, 294, 386, 494, 507
SfaNI GCATC 3 cut(s) 237, 287, 522
SgrAI CRCCGGYG 1 cut(s) 116
SgrDI CGTCGACG 1 cut(s) 89
SmlI CTYRAG 3 cut(s) 151, 168, 215
SmoI CTYRAG 3 cut(s) 151, 168, 215
SpeI ACTAGT 1 cut(s) 101
Sse9I AATT 1 cut(s) 529
SsiI CCGC 3 cut(s) 114, 321, 496
SspI AATATT 1 cut(s) 398
SspMI CTAG 1 cut(s) 102
SstI GAGCTC 1 cut(s) 294
StyD4I CCNGG 1 cut(s) 243
TaaI ACNGT 3 cut(s) 11, 18, 458
TaiI ACGT 3 cut(s) 90, 386, 494
TaqI TCGA 1 cut(s) 90
TasI AATT 1 cut(s) 529
TatI WGTACW 2 cut(s) 34, 350
TauI GCSGC 2 cut(s) 324, 499
TfiI GAWTC 4 cut(s) 124, 136, 206, 448
Tru1I TTAA 5 cut(s) 152, 216, 333, 528, 532
Tru9I TTAA 5 cut(s) 152, 216, 333, 528, 532
TscAI CASTG 2 cut(s) 489, 523
TseFI GTSAC 1 cut(s) 452
Tsp45I GTSAC 1 cut(s) 452
TspGWI ACGGA 1 cut(s) 53
TspRI CASTG 2 cut(s) 489, 523
Vha464I CTTAAG 2 cut(s) 151, 215
VspI ATTAAT 1 cut(s) 333
XmiI GTMKAC 1 cut(s) 90
XspI CTAG 1 cut(s) 102
ZrmI AGTACT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.