MD11G1058700.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0014200
Physical Location & Seq
Forward (+)
5005909 .. 5006361
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1058700.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGGCAACAGTCTAAGGTGCTGTTTGGCTTGCGTACTACCATGTGGAGCCCTAGACTTGATCCGGATCGTCCATTTGAATGGCTACATAGAAGAAATCTCACATCCAGTTACAGCCGGCGAGATCTTAGAAGCAAACCCTAATCACCTTCTCAGCAAACCCAACTCCCAAGGGGTCGTGCGCAAAATTTTGATCCTCTCGCCGGAGTCGGAGCTCAAGCGAGGCAGCATCTATTTCTTGATTCCGTCTTCTTCTGTGCCGGAGAAGAAGAAGAAGAGTAAGAGCAGCGGCAATAAGAAACCCTCAAAAAAGAGCAGCAAGAGCCAAAGCGATAGTACCGACCGTTACTTGACGGAGATTGTGACGGAAAAGAAGTCTATGTCTCGCCGGGATAGGAGGGCTGGCCGTGTCACGGTATGGCGGCCTCATCTCGAAAGCATCACCGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

151

Amino Acids

16.68

Weight (kDa)

9.66

Isoelectric Point (pI)

65.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 149 3.1e-39 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06980 AT2G30230
fragaria_vesca FvH4_3g39820
malus_domestica MD03G1057000.v1.1 MD11G1058700.v1.1
prunus_persica Prupe.6G047800_v2.0.a1
pyrus_communis pycom03g04530 pycom11g04880
rosa_chinensis RchiOBHm_Chr5g0071711
rosa_laevigata RLG00000036243
rosa_multiflora Rmu_sc0022933.1_g000001
rosa_roxburghii Rroxscaffold_1G00009470
rosa_rugosa Rorug05G0414300
rosa_samantha Rh5BG490000 Rh5CG514000 Rh5DG500800
rosa_wichuraiana Rw5G043710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 182
AccIII TCCGGA 1 cut(s) 63
AciI CCGC 2 cut(s) 288, 421
AclWI GGATC 3 cut(s) 55, 74, 187
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 1 cut(s) 186
AfaI GTAC 2 cut(s) 36, 337
AfiI CCNNNNNNNGG 2 cut(s) 202, 412
AgsI TTSAA 1 cut(s) 79
AluBI AGCT 1 cut(s) 214
AluI AGCT 1 cut(s) 214
Alw21I GWGCWC 1 cut(s) 216
Alw26I GTCTC 1 cut(s) 387
AlwI GGATC 3 cut(s) 55, 74, 187
Aor13HI TCCGGA 1 cut(s) 63
AoxI GGCC 2 cut(s) 403, 422
ApeKI GCWGC 3 cut(s) 225, 285, 315
ApoI RAATTY 1 cut(s) 186
AspLEI GCGC 1 cut(s) 183
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 2 cut(s) 137, 433
BanII GRGCYC 2 cut(s) 52, 216
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 3 cut(s) 237, 297, 327
BceAI ACGGC 1 cut(s) 390
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 387
BfaI CTAG 1 cut(s) 53
BglII AGATCT 1 cut(s) 123
BisI GCNGC 5 cut(s) 226, 286, 289, 316, 422
BlsI GCNGC 5 cut(s) 227, 287, 290, 317, 423
Bme1390I CCNGG 1 cut(s) 389
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 2 cut(s) 237, 447
BpiI GAAGAC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 389
BsaBI GATNNNNATC 1 cut(s) 65
BsaJI CCNNGG 1 cut(s) 169
BsaWI WCCGGW 1 cut(s) 63
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 412
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 65
BseAI TCCGGA 1 cut(s) 63
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 103
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 2 cut(s) 202, 412
BseMII CTCAG 1 cut(s) 166
BseNI ACTGG 1 cut(s) 107
BseXI GCAGC 3 cut(s) 237, 297, 327
Bsh1285I CGRYCG 1 cut(s) 343
BshFI GGCC 2 cut(s) 405, 424
BsiEI CGRYCG 1 cut(s) 343
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 5 cut(s) 64, 117, 203, 260, 388
BslI CCNNNNNNNGG 2 cut(s) 202, 412
BsmAI GTCTC 1 cut(s) 387
BsnI GGCC 2 cut(s) 405, 424
Bsp1286I GDGCHC 2 cut(s) 52, 216
Bsp13I TCCGGA 1 cut(s) 63
Bsp143I GATC 4 cut(s) 60, 66, 123, 192
BspACI CCGC 2 cut(s) 288, 421
BspANI GGCC 2 cut(s) 405, 424
BspCNI CTCAG 1 cut(s) 165
BspEI TCCGGA 1 cut(s) 63
BspLI GGNNCC 1 cut(s) 49
BspPI GGATC 3 cut(s) 55, 74, 187
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 107
BssAI RCCGGY 1 cut(s) 116
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 4 cut(s) 60, 66, 123, 192
BssT1I CCWWGG 1 cut(s) 169
Bst4CI ACNGT 3 cut(s) 11, 344, 415
Bst6I CTCTTC 1 cut(s) 269
BstC8I GCNNGC 3 cut(s) 31, 118, 403
BstDEI CTNAG 3 cut(s) 14, 127, 152
BstF5I GGATG 1 cut(s) 103
BstHHI GCGC 1 cut(s) 183
BstKTI GATC 4 cut(s) 63, 69, 126, 195
BstMAI GTCTC 1 cut(s) 387
BstMBI GATC 4 cut(s) 60, 66, 123, 192
BstMCI CGRYCG 1 cut(s) 343
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstSCI CCNGG 1 cut(s) 387
BstV1I GCAGC 3 cut(s) 237, 297, 327
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 123
BstXI CCANNNNNNTGG 1 cut(s) 80
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 405, 424
BtsCI GGATG 1 cut(s) 103
Cac8I GCNNGC 3 cut(s) 31, 118, 403
CfoI GCGC 1 cut(s) 183
Cfr10I RCCGGY 1 cut(s) 116
Csp6I GTAC 2 cut(s) 35, 336
CviAII CATG 1 cut(s) 42
CviJI RGCY 9 cut(s) 29, 50, 84, 116, 214, 324, 401, 405, 424
CviKI_1 RGCY 9 cut(s) 29, 50, 84, 116, 214, 324, 401, 405, 424
CviQI GTAC 2 cut(s) 35, 336
DdeI CTNAG 3 cut(s) 14, 127, 152
DpnI GATC 4 cut(s) 62, 68, 125, 194
DpnII GATC 4 cut(s) 60, 66, 123, 192
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 269
EarI CTCTTC 1 cut(s) 269
Ecl136II GAGCTC 1 cut(s) 214
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 52, 216
Eco53kI GAGCTC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 169
EcoT38I GRGCYC 2 cut(s) 52, 216
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 1 cut(s) 45
FaiI YATR 4 cut(s) 43, 89, 380, 418
FatI CATG 1 cut(s) 41
Fnu4HI GCNGC 5 cut(s) 226, 286, 289, 316, 422
FokI GGATG 1 cut(s) 90
FriOI GRGCYC 2 cut(s) 52, 216
Fsp4HI GCNGC 5 cut(s) 226, 286, 289, 316, 422
FspBI CTAG 1 cut(s) 53
FspI TGCGCA 1 cut(s) 182
GlaI GCGC 1 cut(s) 182
GluI GCNGC 5 cut(s) 226, 286, 289, 316, 422
HaeIII GGCC 2 cut(s) 405, 424
HapII CCGG 5 cut(s) 64, 117, 203, 260, 388
HhaI GCGC 1 cut(s) 183
Hin1II CATG 1 cut(s) 45
Hin6I GCGC 1 cut(s) 181
HinP1I GCGC 1 cut(s) 181
HinfI GANTC 2 cut(s) 206, 241
HpaII CCGG 5 cut(s) 64, 117, 203, 260, 388
HphI GGTGA 2 cut(s) 137, 433
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 3 cut(s) 64, 238, 431
HpyAV CCTTC 1 cut(s) 158
HpyCH4III ACNGT 3 cut(s) 11, 344, 415
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 3 cut(s) 14, 127, 152
Hsp92II CATG 1 cut(s) 45
HspAI GCGC 1 cut(s) 181
Kpn2I TCCGGA 1 cut(s) 63
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 4 cut(s) 60, 66, 123, 192
LmnI GCTCC 2 cut(s) 47, 211
LpnPI CCDG 7 cut(s) 77, 120, 130, 216, 273, 387, 401
Lsp1109I GCAGC 3 cut(s) 237, 297, 327
LweI GCATC 2 cut(s) 237, 447
MaeI CTAG 1 cut(s) 53
MaeIII GTNAC 4 cut(s) 109, 344, 361, 409
MalI GATC 4 cut(s) 62, 68, 125, 194
MboI GATC 4 cut(s) 60, 66, 123, 192
MboII GAAGA 7 cut(s) 104, 240, 243, 277, 280, 283, 286
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 52, 216
MluCI AATT 1 cut(s) 186
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 5 cut(s) 206, 215, 313, 390, 435
MroI TCCGGA 1 cut(s) 63
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 1 cut(s) 451
MslI CAYNNNNRTG 1 cut(s) 78
MspA1I CMGCKG 1 cut(s) 288
MspI CCGG 5 cut(s) 64, 117, 203, 260, 388
MspR9I CCNGG 1 cut(s) 389
MwoI GCNNNNNNNGC 1 cut(s) 321
NaeI GCCGGC 1 cut(s) 118
NciI CCSGG 1 cut(s) 389
NdeII GATC 4 cut(s) 60, 66, 123, 192
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 45
NlaIV GGNNCC 1 cut(s) 49
NmuCI GTSAC 2 cut(s) 361, 409
NsbI TGCGCA 1 cut(s) 182
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 1 cut(s) 241
PkrI GCNGC 5 cut(s) 227, 287, 290, 317, 423
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 49
PsuI RGATCY 1 cut(s) 123
RsaI GTAC 2 cut(s) 36, 337
RsaNI GTAC 2 cut(s) 35, 336
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 451
SatI GCNGC 5 cut(s) 226, 286, 289, 316, 422
Sau3AI GATC 4 cut(s) 60, 66, 123, 192
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 2 cut(s) 52, 216
SetI ASST 3 cut(s) 20, 150, 216
SfaNI GCATC 2 cut(s) 237, 447
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
Sse9I AATT 1 cut(s) 186
SsiI CCGC 2 cut(s) 288, 421
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 387
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 3 cut(s) 11, 344, 415
TaqI TCGA 1 cut(s) 432
TasI AATT 1 cut(s) 186
TauI GCSGC 2 cut(s) 291, 424
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 1 cut(s) 451
Tru9I TTAA 1 cut(s) 451
TseFI GTSAC 2 cut(s) 361, 409
TseI GCWGC 3 cut(s) 225, 285, 315
Tsp45I GTSAC 2 cut(s) 361, 409
TspGWI ACGGA 3 cut(s) 234, 368, 380
XapI RAATTY 1 cut(s) 186
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.