FvH4_3g39820

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014200
Physical Location & Seq
Forward (+)
33743879 .. 33745015
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39820.t1

Sequence Viewer

Length: 474 bp
ATGGGCAACAGTTTAAGGTGTTGTTTAGCTTGTGTTCTACCTTGTGGAGCTCTAGACTTGATCCGAATAGTCCATTTAAACGGCTACGTAGAAGAAATCACACGTCCGATCACTGCCGGCGAGATCCTTGCCGCCAATCCAAATCACGTTCTGAGCAAGCCATGTTCACAAGGCGTCGTTCGCCGCATTTTGATTCTGTCGCCGGAGTCGGAGCTCAAGCGAGGAAGCATATACTTCTTGATTCCGGAGTCTTCGGTACCGGACAGTAAGAAGAAGAAGAGCAAAGGTAGTAGCGGCGTTAAGAAGTCGTCAAAGAAGAGACTAAGCAGTGTTGATGCCAATGTCTCCGAGTGTGATCGATACTTGACGGAGATCGTAACGGAAAATAAAAAATCCTCATCACGTAGGGATCGCCGGACCGGACGATCACTCGGAGCATTTCAGCCTCATCTTGAGAGCATTTCTGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

158

Amino Acids

17.21

Weight (kDa)

9.45

Isoelectric Point (pI)

67.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 156 5.8e-37 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06980 AT2G30230
fragaria_vesca FvH4_3g39820
malus_domestica MD03G1057000.v1.1 MD11G1058700.v1.1
prunus_persica Prupe.6G047800_v2.0.a1
pyrus_communis pycom03g04530 pycom11g04880
rosa_chinensis RchiOBHm_Chr5g0071711
rosa_laevigata RLG00000036243
rosa_multiflora Rmu_sc0022933.1_g000001
rosa_roxburghii Rroxscaffold_1G00009470
rosa_rugosa Rorug05G0414300
rosa_samantha Rh5BG490000 Rh5CG514000 Rh5DG500800
rosa_wichuraiana Rw5G043710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 256
AccB1I GGYRCC 1 cut(s) 256
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 3 cut(s) 132, 184, 294
AclWI GGATC 3 cut(s) 55, 118, 417
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 1 cut(s) 258
AflIII ACRYGT 1 cut(s) 101
AjiI CACGTC 1 cut(s) 104
AluBI AGCT 3 cut(s) 29, 50, 214
AluI AGCT 3 cut(s) 29, 50, 214
Alw21I GWGCWC 2 cut(s) 52, 216
Alw26I GTCTC 2 cut(s) 313, 349
AlwI GGATC 3 cut(s) 55, 118, 417
Aor13HI TCCGGA 1 cut(s) 244
Asp718I GGTACC 1 cut(s) 256
AspS9I GGNCC 1 cut(s) 417
AvaII GGWCC 1 cut(s) 417
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 2 cut(s) 52, 216
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 2 cut(s) 52, 216
BceAI ACGGC 1 cut(s) 97
BcgI CGANNNNNNTGC 2 cut(s) 110, 144
BcoDI GTCTC 2 cut(s) 313, 349
BfaI CTAG 2 cut(s) 53, 472
BisI GCNGC 3 cut(s) 132, 184, 295
BlsI GCNGC 3 cut(s) 133, 185, 296
Bme18I GGWCC 1 cut(s) 417
BmgBI CACGTC 1 cut(s) 104
BmgT120I GGNCC 1 cut(s) 417
BmiI GGNNCC 1 cut(s) 258
BmsI GCATC 1 cut(s) 325
BpiI GAAGAC 1 cut(s) 243
BpuEI CTTGAG 2 cut(s) 200, 473
Bsa29I ATCGAT 1 cut(s) 358
BsaAI YACGTR 2 cut(s) 88, 404
BsaHI GRCGYC 1 cut(s) 174
BsaWI WCCGGW 3 cut(s) 244, 259, 419
Bse118I RCCGGY 1 cut(s) 116
BseAI TCCGGA 1 cut(s) 244
BseCI ATCGAT 1 cut(s) 358
BseMII CTCAG 1 cut(s) 143
BshNI GGYRCC 1 cut(s) 256
BshVI ATCGAT 1 cut(s) 358
BsiHKAI GWGCWC 2 cut(s) 52, 216
BsiSI CCGG 6 cut(s) 117, 203, 245, 260, 415, 420
BsmAI GTCTC 2 cut(s) 313, 349
Bsp1286I GDGCHC 2 cut(s) 52, 216
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
BspACI CCGC 3 cut(s) 132, 184, 294
BspCNI CTCAG 1 cut(s) 144
BspDI ATCGAT 1 cut(s) 358
BspEI TCCGGA 1 cut(s) 244
BspLI GGNNCC 1 cut(s) 258
BspPI GGATC 3 cut(s) 55, 118, 417
BspQI GCTCTTC 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 256
BsrFI RCCGGY 1 cut(s) 116
BssAI RCCGGY 1 cut(s) 116
BssMI GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
BssNI GRCGYC 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 11, 266
Bst6I CTCTTC 2 cut(s) 272, 311
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 2 cut(s) 88, 404
BstC8I GCNNGC 2 cut(s) 118, 158
BstDEI CTNAG 2 cut(s) 152, 323
BstKTI GATC 7 cut(s) 63, 111, 126, 358, 375, 412, 428
BstMAI GTCTC 2 cut(s) 313, 349
BstMBI GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstSNI TACGTA 1 cut(s) 88
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
Bsu15I ATCGAT 1 cut(s) 358
BsuTUI ATCGAT 1 cut(s) 358
BtrI CACGTC 1 cut(s) 104
BtsI GCAGTG 2 cut(s) 111, 334
BtsIMutI CAGTG 2 cut(s) 111, 334
Cac8I GCNNGC 2 cut(s) 118, 158
Cfr10I RCCGGY 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 417
ClaI ATCGAT 1 cut(s) 358
CpoI CGGWCCG 1 cut(s) 417
CseI GACGC 1 cut(s) 163
Csp6I GTAC 1 cut(s) 257
CspI CGGWCCG 1 cut(s) 417
CviAII CATG 1 cut(s) 162
CviJI RGCY 6 cut(s) 29, 50, 84, 160, 214, 445
CviKI_1 RGCY 6 cut(s) 29, 50, 84, 160, 214, 445
CviQI GTAC 1 cut(s) 257
DdeI CTNAG 2 cut(s) 152, 323
DpnI GATC 7 cut(s) 62, 110, 125, 357, 374, 411, 427
DpnII GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
DraI TTTAAA 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 272, 311
EarI CTCTTC 2 cut(s) 272, 311
Ecl136II GAGCTC 2 cut(s) 50, 214
Eco105I TACGTA 1 cut(s) 88
Eco24I GRGCYC 2 cut(s) 52, 216
Eco47I GGWCC 1 cut(s) 417
Eco53kI GAGCTC 2 cut(s) 50, 214
EcoICRI GAGCTC 2 cut(s) 50, 214
EcoT38I GRGCYC 2 cut(s) 52, 216
FaeI CATG 1 cut(s) 165
FaiI YATR 3 cut(s) 163, 230, 232
FatI CATG 1 cut(s) 161
Fnu4HI GCNGC 3 cut(s) 132, 184, 295
FriOI GRGCYC 2 cut(s) 52, 216
Fsp4HI GCNGC 3 cut(s) 132, 184, 295
FspBI CTAG 2 cut(s) 53, 472
GluI GCNGC 3 cut(s) 132, 184, 295
HapII CCGG 6 cut(s) 117, 203, 245, 260, 415, 420
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 1 cut(s) 165
HinfI GANTC 4 cut(s) 193, 206, 241, 248
HpaII CCGG 6 cut(s) 117, 203, 245, 260, 415, 420
Hpy166II GTNNAC 1 cut(s) 167
Hpy188I TCNGA 7 cut(s) 65, 108, 153, 211, 349, 434, 466
Hpy188III TCNNGA 4 cut(s) 53, 238, 245, 452
Hpy8I GTNNAC 1 cut(s) 167
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 2 cut(s) 11, 266
HpyCH4IV ACGT 4 cut(s) 87, 103, 147, 403
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 2 cut(s) 152, 323
HpySE526I ACGT 4 cut(s) 87, 103, 147, 403
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 1 cut(s) 165
Kpn2I TCCGGA 1 cut(s) 244
KpnI GGTACC 1 cut(s) 260
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
LguI GCTCTTC 1 cut(s) 272
LmnI GCTCC 3 cut(s) 47, 211, 434
LpnPI CCDG 6 cut(s) 130, 216, 258, 273, 428, 433
LweI GCATC 1 cut(s) 325
MaeI CTAG 2 cut(s) 53, 472
MaeII ACGT 4 cut(s) 87, 103, 147, 403
MaeIII GTNAC 1 cut(s) 376
MalI GATC 7 cut(s) 62, 110, 125, 357, 374, 411, 427
MboI GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
MboII GAAGA 6 cut(s) 104, 243, 283, 286, 289, 328
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 52, 216
MlyI GAGTC 2 cut(s) 215, 257
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 3 cut(s) 215, 406, 456
MroI TCCGGA 1 cut(s) 244
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 3 cut(s) 14, 77, 300
MspI CCGG 6 cut(s) 117, 203, 245, 260, 415, 420
MwoI GCNNNNNNNGC 1 cut(s) 180
NaeI GCCGGC 1 cut(s) 118
NdeII GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
NgoMIV GCCGGC 1 cut(s) 116
NlaIII CATG 1 cut(s) 165
NlaIV GGNNCC 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 272
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 2 cut(s) 193, 241
PkrI GCNGC 3 cut(s) 133, 185, 296
PleI GAGTC 2 cut(s) 214, 256
PpsI GAGTC 2 cut(s) 214, 256
Ppu21I YACGTR 2 cut(s) 88, 404
Psp124BI GAGCTC 2 cut(s) 52, 216
PspN4I GGNNCC 1 cut(s) 258
PspPI GGNCC 1 cut(s) 417
PsuI RGATCY 1 cut(s) 123
RsaI GTAC 1 cut(s) 258
RsaNI GTAC 1 cut(s) 257
Rsr2I CGGWCCG 1 cut(s) 417
RsrII CGGWCCG 1 cut(s) 417
SacI GAGCTC 2 cut(s) 52, 216
SapI GCTCTTC 1 cut(s) 272
SaqAI TTAA 3 cut(s) 14, 77, 300
SatI GCNGC 3 cut(s) 132, 184, 295
Sau3AI GATC 7 cut(s) 60, 108, 123, 355, 372, 409, 425
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 2 cut(s) 215, 257
SduI GDGCHC 2 cut(s) 52, 216
SfaNI GCATC 1 cut(s) 325
SinI GGWCC 1 cut(s) 417
SmlI CTYRAG 2 cut(s) 215, 452
SmoI CTYRAG 2 cut(s) 215, 452
SnaBI TACGTA 1 cut(s) 88
SsiI CCGC 3 cut(s) 132, 184, 294
SspMI CTAG 2 cut(s) 53, 472
SstI GAGCTC 2 cut(s) 52, 216
TaaI ACNGT 2 cut(s) 11, 266
TaiI ACGT 4 cut(s) 90, 106, 150, 406
TaqI TCGA 1 cut(s) 358
TauI GCSGC 3 cut(s) 134, 186, 297
TfiI GAWTC 2 cut(s) 193, 241
Tru1I TTAA 3 cut(s) 14, 77, 300
Tru9I TTAA 3 cut(s) 14, 77, 300
TscAI CASTG 2 cut(s) 118, 334
TspGWI ACGGA 2 cut(s) 383, 395
TspRI CASTG 2 cut(s) 118, 334
VpaK11BI GGWCC 1 cut(s) 417
XbaI TCTAGA 1 cut(s) 52
XspI CTAG 2 cut(s) 53, 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.