MD03G1057000.v1.1

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014200
Physical Location & Seq
Forward (+)
4548716 .. 4549168
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1057000.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGGCAACAGTCTAAGGTGCTGTTTGGCTTGCGTACTACCGTGTGGAGCCCTAGACTTGATACGGATCGTCCATTTGAATGGCTACGTAGAAGAAATCTCACATCCAGTCACTGCTGGTGAGATCTTGGAAGCAAACCCTAATCATGTTCTCAGCAAACCCAGCTCCCAAGGCGTCGTACGCAAAATTTTGATCCTCTCGCCGGAGTCGGAGCTCAAGCGTGGCAGCATCTATTTCTTGATTCCGTCTTCTTCTGTGCCGGAGAAGAGGAAAAAGAGTAAGAGCAACGGCATTAAGAAACCCTCAAAAAAGAGCAGCAAGAGCCAAAGCGATAGTACTGACCGTTACTTGACGGAGATTGTGACGGAAAAGAAGTTTACCTCTCGCCGGGATCGGAGGGCTGGCCGTGTCGCAGTATGGCGGCCTAATCTCGAAAGCATCACCGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

151

Amino Acids

16.66

Weight (kDa)

9.69

Isoelectric Point (pI)

60.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 149 2.8e-38 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06980 AT2G30230
fragaria_vesca FvH4_3g39820
malus_domestica MD03G1057000.v1.1 MD11G1058700.v1.1
prunus_persica Prupe.6G047800_v2.0.a1
pyrus_communis pycom03g04530 pycom11g04880
rosa_chinensis RchiOBHm_Chr5g0071711
rosa_laevigata RLG00000036243
rosa_multiflora Rmu_sc0022933.1_g000001
rosa_roxburghii Rroxscaffold_1G00009470
rosa_rugosa Rorug05G0414300
rosa_samantha Rh5BG490000 Rh5CG514000 Rh5DG500800
rosa_wichuraiana Rw5G043710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 421
AclWI GGATC 3 cut(s) 74, 187, 399
AcoI YGGCCR 1 cut(s) 403
AcsI RAATTY 1 cut(s) 186
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 3 cut(s) 36, 180, 337
AfiI CCNNNNNNNGG 2 cut(s) 202, 387
AgsI TTSAA 1 cut(s) 79
AluBI AGCT 2 cut(s) 165, 214
AluI AGCT 2 cut(s) 165, 214
Alw21I GWGCWC 1 cut(s) 216
AlwI GGATC 3 cut(s) 74, 187, 399
AlwNI CAGNNNCTG 1 cut(s) 113
AoxI GGCC 2 cut(s) 403, 422
ApeKI GCWGC 2 cut(s) 225, 315
ApoI RAATTY 1 cut(s) 186
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 2 cut(s) 131, 433
BanII GRGCYC 2 cut(s) 52, 216
BbsI GAAGAC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 216
BbvI GCAGC 2 cut(s) 237, 327
BceAI ACGGC 2 cut(s) 304, 390
BcnI CCSGG 1 cut(s) 389
BfaI CTAG 1 cut(s) 53
BglII AGATCT 1 cut(s) 123
BisI GCNGC 3 cut(s) 226, 316, 422
BlsI GCNGC 3 cut(s) 227, 317, 423
BmcAI AGTACT 1 cut(s) 337
Bme1390I CCNGG 1 cut(s) 389
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 2 cut(s) 237, 447
BpiI GAAGAC 1 cut(s) 240
BpuEI CTTGAG 1 cut(s) 200
BpuMI CCSGG 1 cut(s) 389
BsaAI YACGTR 1 cut(s) 88
BsaBI GATNNNNATC 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 169
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 387
Bse1I ACTGG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 65
BseDI CCNNGG 1 cut(s) 169
BseGI GGATG 1 cut(s) 103
BseJI GATNNNNATC 1 cut(s) 65
BseLI CCNNNNNNNGG 2 cut(s) 202, 387
BseMII CTCAG 1 cut(s) 166
BseNI ACTGG 1 cut(s) 107
BseXI GCAGC 2 cut(s) 237, 327
BseYI CCCAGC 1 cut(s) 161
BshFI GGCC 2 cut(s) 405, 424
BsiHKAI GWGCWC 1 cut(s) 216
BsiSI CCGG 3 cut(s) 203, 260, 388
BsiWI CGTACG 1 cut(s) 178
BslI CCNNNNNNNGG 2 cut(s) 202, 387
BsnI GGCC 2 cut(s) 405, 424
Bsp1286I GDGCHC 2 cut(s) 52, 216
Bsp143I GATC 4 cut(s) 66, 123, 192, 391
BspACI CCGC 1 cut(s) 421
BspANI GGCC 2 cut(s) 405, 424
BspCNI CTCAG 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 49
BspPI GGATC 3 cut(s) 74, 187, 399
BsrI ACTGG 1 cut(s) 107
BssECI CCNNGG 1 cut(s) 169
BssMI GATC 4 cut(s) 66, 123, 192, 391
BssNI GRCGYC 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 169
Bst4CI ACNGT 3 cut(s) 11, 42, 344
Bst6I CTCTTC 1 cut(s) 260
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 31, 403
BstDEI CTNAG 2 cut(s) 14, 152
BstF5I GGATG 1 cut(s) 103
BstKTI GATC 4 cut(s) 69, 126, 195, 394
BstMBI GATC 4 cut(s) 66, 123, 192, 391
BstMWI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 321
BstSCI CCNGG 1 cut(s) 387
BstSNI TACGTA 1 cut(s) 88
BstV1I GCAGC 2 cut(s) 237, 327
BstV2I GAAGAC 1 cut(s) 240
BstX2I RGATCY 1 cut(s) 123
BstXI CCANNNNNNTGG 1 cut(s) 80
BstYI RGATCY 1 cut(s) 123
BsuRI GGCC 2 cut(s) 405, 424
BtsCI GGATG 1 cut(s) 103
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 2 cut(s) 31, 403
CaiI CAGNNNCTG 1 cut(s) 113
CseI GACGC 1 cut(s) 163
Csp6I GTAC 3 cut(s) 35, 179, 336
CviAII CATG 1 cut(s) 146
CviJI RGCY 9 cut(s) 29, 50, 84, 165, 214, 324, 401, 405, 424
CviKI_1 RGCY 9 cut(s) 29, 50, 84, 165, 214, 324, 401, 405, 424
CviQI GTAC 3 cut(s) 35, 179, 336
DdeI CTNAG 2 cut(s) 14, 152
DpnI GATC 4 cut(s) 68, 125, 194, 393
DpnII GATC 4 cut(s) 66, 123, 192, 391
EaeI YGGCCR 1 cut(s) 403
Eam1104I CTCTTC 1 cut(s) 260
EarI CTCTTC 1 cut(s) 260
Ecl136II GAGCTC 1 cut(s) 214
Eco105I TACGTA 1 cut(s) 88
Eco130I CCWWGG 1 cut(s) 169
Eco24I GRGCYC 2 cut(s) 52, 216
Eco53kI GAGCTC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 214
EcoT14I CCWWGG 1 cut(s) 169
EcoT38I GRGCYC 2 cut(s) 52, 216
ErhI CCWWGG 1 cut(s) 169
FaeI CATG 1 cut(s) 149
FaiI YATR 2 cut(s) 147, 418
FatI CATG 1 cut(s) 145
Fnu4HI GCNGC 3 cut(s) 226, 316, 422
FokI GGATG 1 cut(s) 90
FriOI GRGCYC 2 cut(s) 52, 216
Fsp4HI GCNGC 3 cut(s) 226, 316, 422
FspBI CTAG 1 cut(s) 53
GluI GCNGC 3 cut(s) 226, 316, 422
GsaI CCCAGC 1 cut(s) 165
HaeIII GGCC 2 cut(s) 405, 424
HapII CCGG 3 cut(s) 203, 260, 388
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 1 cut(s) 149
HinfI GANTC 2 cut(s) 206, 241
HpaII CCGG 3 cut(s) 203, 260, 388
HphI GGTGA 2 cut(s) 131, 433
Hpy166II GTNNAC 1 cut(s) 378
Hpy188I TCNGA 2 cut(s) 211, 396
Hpy188III TCNNGA 2 cut(s) 238, 431
Hpy8I GTNNAC 1 cut(s) 378
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 3 cut(s) 11, 42, 344
HpyCH4IV ACGT 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 321
HpyF3I CTNAG 2 cut(s) 14, 152
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 1 cut(s) 149
Kzo9I GATC 4 cut(s) 66, 123, 192, 391
LmnI GCTCC 3 cut(s) 47, 170, 211
LpnPI CCDG 7 cut(s) 102, 120, 175, 216, 273, 387, 401
Lsp1109I GCAGC 2 cut(s) 237, 327
LweI GCATC 2 cut(s) 237, 447
MaeI CTAG 1 cut(s) 53
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 3 cut(s) 109, 344, 361
MalI GATC 4 cut(s) 68, 125, 194, 393
MboI GATC 4 cut(s) 66, 123, 192, 391
MboII GAAGA 4 cut(s) 104, 240, 243, 277
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 52, 216
MluCI AATT 1 cut(s) 186
MlyI GAGTC 1 cut(s) 215
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 5 cut(s) 206, 261, 313, 390, 391
MseI TTAA 1 cut(s) 294
MslI CAYNNNNRTG 1 cut(s) 78
MspI CCGG 3 cut(s) 203, 260, 388
MspR9I CCNGG 1 cut(s) 389
MwoI GCNNNNNNNGC 4 cut(s) 162, 171, 180, 321
NciI CCSGG 1 cut(s) 389
NdeII GATC 4 cut(s) 66, 123, 192, 391
NlaIII CATG 1 cut(s) 149
NlaIV GGNNCC 1 cut(s) 49
NmuCI GTSAC 2 cut(s) 109, 361
PfeI GAWTC 1 cut(s) 241
Pfl23II CGTACG 1 cut(s) 178
PkrI GCNGC 3 cut(s) 227, 317, 423
PleI GAGTC 1 cut(s) 214
PpsI GAGTC 1 cut(s) 214
Ppu21I YACGTR 1 cut(s) 88
Psp124BI GAGCTC 1 cut(s) 216
PspFI CCCAGC 1 cut(s) 161
PspLI CGTACG 1 cut(s) 178
PspN4I GGNNCC 1 cut(s) 49
PstNI CAGNNNCTG 1 cut(s) 113
PsuI RGATCY 1 cut(s) 123
RsaI GTAC 3 cut(s) 36, 180, 337
RsaNI GTAC 3 cut(s) 35, 179, 336
RseI CAYNNNNRTG 1 cut(s) 78
SacI GAGCTC 1 cut(s) 216
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 3 cut(s) 226, 316, 422
Sau3AI GATC 4 cut(s) 66, 123, 192, 391
ScaI AGTACT 1 cut(s) 337
SchI GAGTC 1 cut(s) 215
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 2 cut(s) 52, 216
SetI ASST 5 cut(s) 20, 90, 167, 216, 383
SfaNI GCATC 2 cut(s) 237, 447
SmiMI CAYNNNNRTG 1 cut(s) 78
SmlI CTYRAG 1 cut(s) 215
SmoI CTYRAG 1 cut(s) 215
SnaBI TACGTA 1 cut(s) 88
Sse9I AATT 1 cut(s) 186
SsiI CCGC 1 cut(s) 421
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 1 cut(s) 216
StyD4I CCNGG 1 cut(s) 387
StyI CCWWGG 1 cut(s) 169
TaaI ACNGT 3 cut(s) 11, 42, 344
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 432
TasI AATT 1 cut(s) 186
TatI WGTACW 1 cut(s) 335
TauI GCSGC 1 cut(s) 424
TfiI GAWTC 1 cut(s) 241
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 2 cut(s) 109, 361
TseI GCWGC 2 cut(s) 225, 315
Tsp45I GTSAC 2 cut(s) 109, 361
TspGWI ACGGA 4 cut(s) 79, 234, 368, 380
TspRI CASTG 1 cut(s) 118
XapI RAATTY 1 cut(s) 186
XspI CTAG 1 cut(s) 53
ZrmI AGTACT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.