Rroxscaffold_1G00009470

Domain of unknown function (DUF4228)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0014200
Physical Location & Seq
Reverse (-)
11988694 .. 11989629
936 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00009470.1

Sequence Viewer

Length: 471 bp
ATGGGCAACAGTCTAAGGTGTTGTTTGGCTTGTGTTCTACCTTGTGGAGCTCTAGACTTGATCCGAATAGTCCATTTAAACGGCTACGTAGAAGAAATCACACGTCCGGTCACTGCCGGCCAGATCCTTGCCGCCAATCCAAATCACTTCCTAAGCAAACCCTGTTCACAAGGCGTCGTTCGCCGCATTTTGATTCTCTCGCCGGAGTCGGAGCTCAAGCGAGGCAACATATACTTCTTGATTCCGGAGTCTTCGATACCGGACAGTAAGAAGAAGAACAAAGGTAGTAGCGGCGTTAAGAAGTCGTCAAAGAAGAGACTAAGTAGTGGTGATGCCAATGTCTCGGAGCGTGATCGATACTTAACGGAGATCGTCTCGGAAAAGAAAAAATCTTCATCACGTAGGGATCGCCGGACTGGACGATCAGTCGGAGCATTTCGGCCTCATCTTGAGAGCATTTCCGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4228

Protein Analysis

156

Amino Acids

17.21

Weight (kDa)

9.84

Isoelectric Point (pI)

64.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PADRE PF14009 1 - 155 6.3e-39 PADRE domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014200)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G06980 AT2G30230
fragaria_vesca FvH4_3g39820
malus_domestica MD03G1057000.v1.1 MD11G1058700.v1.1
prunus_persica Prupe.6G047800_v2.0.a1
pyrus_communis pycom03g04530 pycom11g04880
rosa_chinensis RchiOBHm_Chr5g0071711
rosa_laevigata RLG00000036243
rosa_multiflora Rmu_sc0022933.1_g000001
rosa_roxburghii Rroxscaffold_1G00009470
rosa_rugosa Rorug05G0414300
rosa_samantha Rh5BG490000 Rh5CG514000 Rh5DG500800
rosa_wichuraiana Rw5G043710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 244
AciI CCGC 3 cut(s) 132, 184, 291
AclWI GGATC 3 cut(s) 55, 118, 414
AcoI YGGCCR 1 cut(s) 118
AcyI GRCGYC 1 cut(s) 174
AflIII ACRYGT 1 cut(s) 101
AhdI GACNNNNNGTC 1 cut(s) 425
AjiI CACGTC 1 cut(s) 104
AluBI AGCT 2 cut(s) 50, 214
AluI AGCT 2 cut(s) 50, 214
Alw21I GWGCWC 2 cut(s) 52, 216
Alw26I GTCTC 3 cut(s) 310, 346, 379
AlwI GGATC 3 cut(s) 55, 118, 414
Aor13HI TCCGGA 1 cut(s) 244
AoxI GGCC 2 cut(s) 118, 440
AsuHPI GGTGA 1 cut(s) 341
BanII GRGCYC 2 cut(s) 52, 216
BbsI GAAGAC 1 cut(s) 243
Bbv12I GWGCWC 2 cut(s) 52, 216
BceAI ACGGC 1 cut(s) 97
BcoDI GTCTC 3 cut(s) 310, 346, 379
BfaI CTAG 1 cut(s) 53
BisI GCNGC 3 cut(s) 132, 184, 292
BlsI GCNGC 3 cut(s) 133, 185, 293
BmeRI GACNNNNNGTC 1 cut(s) 425
BmgBI CACGTC 1 cut(s) 104
BmsI GCATC 1 cut(s) 322
BpiI GAAGAC 1 cut(s) 243
BplI GAGNNNNNCTC 2 cut(s) 359, 391
Bpu10I CCTNAGC 1 cut(s) 152
BpuEI CTTGAG 2 cut(s) 200, 470
Bsa29I ATCGAT 1 cut(s) 355
BsaAI YACGTR 2 cut(s) 88, 401
BsaHI GRCGYC 1 cut(s) 174
BsaWI WCCGGW 3 cut(s) 106, 244, 259
Bse118I RCCGGY 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 421
BseAI TCCGGA 1 cut(s) 244
BseCI ATCGAT 1 cut(s) 355
BseNI ACTGG 1 cut(s) 421
BshFI GGCC 2 cut(s) 120, 442
BshVI ATCGAT 1 cut(s) 355
BsiHKAI GWGCWC 2 cut(s) 52, 216
BsiSI CCGG 6 cut(s) 107, 117, 203, 245, 260, 412
BsmAI GTCTC 3 cut(s) 310, 346, 379
BsmBI CGTCTC 1 cut(s) 379
BsnI GGCC 2 cut(s) 120, 442
Bsp1286I GDGCHC 2 cut(s) 52, 216
Bsp13I TCCGGA 1 cut(s) 244
Bsp143I GATC 6 cut(s) 60, 123, 352, 369, 406, 422
BspACI CCGC 3 cut(s) 132, 184, 291
BspANI GGCC 2 cut(s) 120, 442
BspDI ATCGAT 1 cut(s) 355
BspEI TCCGGA 1 cut(s) 244
BspPI GGATC 3 cut(s) 55, 118, 414
BsrFI RCCGGY 1 cut(s) 116
BsrI ACTGG 1 cut(s) 421
BssAI RCCGGY 1 cut(s) 116
BssMI GATC 6 cut(s) 60, 123, 352, 369, 406, 422
BssNI GRCGYC 1 cut(s) 174
Bst4CI ACNGT 2 cut(s) 11, 266
Bst6I CTCTTC 1 cut(s) 308
BstACI GRCGYC 1 cut(s) 174
BstBAI YACGTR 2 cut(s) 88, 401
BstC8I GCNNGC 1 cut(s) 118
BstDEI CTNAG 3 cut(s) 14, 152, 320
BstKTI GATC 6 cut(s) 63, 126, 355, 372, 409, 425
BstMAI GTCTC 3 cut(s) 310, 346, 379
BstMBI GATC 6 cut(s) 60, 123, 352, 369, 406, 422
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstSNI TACGTA 1 cut(s) 88
BstV2I GAAGAC 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 123
BstYI RGATCY 1 cut(s) 123
Bsu15I ATCGAT 1 cut(s) 355
BsuRI GGCC 2 cut(s) 120, 442
BsuTUI ATCGAT 1 cut(s) 355
BtrI CACGTC 1 cut(s) 104
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 1 cut(s) 111
Cac8I GCNNGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 116
ClaI ATCGAT 1 cut(s) 355
CseI GACGC 1 cut(s) 163
CviJI RGCY 6 cut(s) 29, 50, 84, 120, 214, 442
CviKI_1 RGCY 6 cut(s) 29, 50, 84, 120, 214, 442
DdeI CTNAG 3 cut(s) 14, 152, 320
DpnI GATC 6 cut(s) 62, 125, 354, 371, 408, 424
DpnII GATC 6 cut(s) 60, 123, 352, 369, 406, 422
DraI TTTAAA 1 cut(s) 78
DriI GACNNNNNGTC 1 cut(s) 425
EaeI YGGCCR 1 cut(s) 118
Eam1104I CTCTTC 1 cut(s) 308
Eam1105I GACNNNNNGTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 308
Ecl136II GAGCTC 2 cut(s) 50, 214
Eco105I TACGTA 1 cut(s) 88
Eco24I GRGCYC 2 cut(s) 52, 216
Eco53kI GAGCTC 2 cut(s) 50, 214
EcoICRI GAGCTC 2 cut(s) 50, 214
EcoT38I GRGCYC 2 cut(s) 52, 216
Esp3I CGTCTC 1 cut(s) 379
FaiI YATR 2 cut(s) 230, 232
Fnu4HI GCNGC 3 cut(s) 132, 184, 292
FriOI GRGCYC 2 cut(s) 52, 216
Fsp4HI GCNGC 3 cut(s) 132, 184, 292
FspBI CTAG 1 cut(s) 53
GluI GCNGC 3 cut(s) 132, 184, 292
HaeIII GGCC 2 cut(s) 120, 442
HapII CCGG 6 cut(s) 107, 117, 203, 245, 260, 412
HgaI GACGC 1 cut(s) 163
Hin1I GRCGYC 1 cut(s) 174
HinfI GANTC 4 cut(s) 193, 206, 241, 248
HpaII CCGG 6 cut(s) 107, 117, 203, 245, 260, 412
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 1 cut(s) 167
Hpy188I TCNGA 6 cut(s) 65, 211, 346, 379, 431, 463
Hpy188III TCNNGA 4 cut(s) 53, 238, 245, 449
Hpy8I GTNNAC 1 cut(s) 167
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 2 cut(s) 11, 266
HpyCH4IV ACGT 3 cut(s) 87, 103, 400
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 3 cut(s) 14, 152, 320
HpySE526I ACGT 3 cut(s) 87, 103, 400
Hsp92I GRCGYC 1 cut(s) 174
Kpn2I TCCGGA 1 cut(s) 244
KroI GCCGGC 1 cut(s) 116
KroNI GCCGGC 1 cut(s) 118
Kzo9I GATC 6 cut(s) 60, 123, 352, 369, 406, 422
LmnI GCTCC 4 cut(s) 47, 211, 346, 431
LpnPI CCDG 9 cut(s) 120, 130, 134, 175, 216, 258, 273, 402, 425
LweI GCATC 1 cut(s) 322
MaeI CTAG 1 cut(s) 53
MaeII ACGT 3 cut(s) 87, 103, 400
MaeIII GTNAC 1 cut(s) 109
MalI GATC 6 cut(s) 62, 125, 354, 371, 408, 424
MboI GATC 6 cut(s) 60, 123, 352, 369, 406, 422
MboII GAAGA 6 cut(s) 104, 243, 283, 286, 325, 384
MflI RGATCY 1 cut(s) 123
MhlI GDGCHC 2 cut(s) 52, 216
MlyI GAGTC 2 cut(s) 215, 257
MmeI TCCRAC 2 cut(s) 189, 409
MnlI CCTC 2 cut(s) 215, 453
MroI TCCGGA 1 cut(s) 244
MroNI GCCGGC 1 cut(s) 116
MseI TTAA 3 cut(s) 77, 297, 362
MspI CCGG 6 cut(s) 107, 117, 203, 245, 260, 412
MwoI GCNNNNNNNGC 1 cut(s) 180
NaeI GCCGGC 1 cut(s) 118
NdeII GATC 6 cut(s) 60, 123, 352, 369, 406, 422
NgoMIV GCCGGC 1 cut(s) 116
NmuCI GTSAC 1 cut(s) 109
PdiI GCCGGC 1 cut(s) 118
PfeI GAWTC 2 cut(s) 193, 241
PkrI GCNGC 3 cut(s) 133, 185, 293
PleI GAGTC 2 cut(s) 214, 256
PpsI GAGTC 2 cut(s) 214, 256
Ppu21I YACGTR 2 cut(s) 88, 401
Psp124BI GAGCTC 2 cut(s) 52, 216
PsuI RGATCY 1 cut(s) 123
SacI GAGCTC 2 cut(s) 52, 216
SaqAI TTAA 3 cut(s) 77, 297, 362
SatI GCNGC 3 cut(s) 132, 184, 292
Sau3AI GATC 6 cut(s) 60, 123, 352, 369, 406, 422
SchI GAGTC 2 cut(s) 215, 257
SduI GDGCHC 2 cut(s) 52, 216
SetI ASST 8 cut(s) 20, 43, 52, 90, 106, 216, 286, 403
SfaNI GCATC 1 cut(s) 322
SmlI CTYRAG 2 cut(s) 215, 449
SmoI CTYRAG 2 cut(s) 215, 449
SnaBI TACGTA 1 cut(s) 88
SsiI CCGC 3 cut(s) 132, 184, 291
SspMI CTAG 1 cut(s) 53
SstI GAGCTC 2 cut(s) 52, 216
TaaI ACNGT 2 cut(s) 11, 266
TaiI ACGT 3 cut(s) 90, 106, 403
TaqI TCGA 2 cut(s) 254, 355
TauI GCSGC 3 cut(s) 134, 186, 294
TfiI GAWTC 2 cut(s) 193, 241
Tru1I TTAA 3 cut(s) 77, 297, 362
Tru9I TTAA 3 cut(s) 77, 297, 362
TscAI CASTG 1 cut(s) 118
TseFI GTSAC 1 cut(s) 109
Tsp45I GTSAC 1 cut(s) 109
TspDTI ATGAA 1 cut(s) 384
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 1 cut(s) 118
XbaI TCTAGA 1 cut(s) 52
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.