AT2G31430

Plant invertase/pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0015949
Physical Location & Seq
Forward (+)
13396042 .. 13397283
1242 bp
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UTR
Exon/CDS
Intron
AT2G31430.1

Sequence Viewer

Length: 540 bp
ATGGCCACAATGCTAATAAACCACATGCTTTTCCTTACGTCGTTGTTAATAGTCGTGTTTCCAGTAGCAAACGCGATTCCAGCCAGAGACATTGACAAGTTATGCAAAGAGACAACTGATGTGCCCTTCTGCCTCAAATACCTTGGGACAGATCCACGTATACCAGCCGCACGTGACCTCACTGACGTGCTTTTAATTGCGATAACGCAGTCAAAAATGCAAGTGGATGACGCAACCACTCATATTGATAGAGTCCGTCGAAAGTTCAATGGTCCTCATGGGAGGAGACGGATCGAGGTTTGCAAGACGAATTACGGAATTGCTTCGGCAAGGTTTCACACAGCTTGGGAACTTGGACTTCAAAAATCGTTTTGGGACGTGGAAAAACTGGCAAGGATTGGCACCAACGCTGTGATCGACTGCGAAAACGTATGGAGAAGGGATGGTCCGATACAAACGTCTCCTCTCACTTTCTATAACATGAATGTTTTCAAGCTTTCTGGAATCATTCTTCTTATTTTCAACAAGCTTGTGACCTAA

Protein Analysis

179

Amino Acids

20.33

Weight (kDa)

9.32

Isoelectric Point (pI)

35.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 31 - 171 7.6e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31425 AT2G31430 AT2G31432 AT2G31432 AT3G55680
rosa_chinensis RchiOBHm_Chr3g0486661
rosa_laevigata RLG00000023068
rosa_multiflora Rmu_sc0000261.1_g000009
rosa_roxburghii Rroxscaffold_6G00395680
rosa_rugosa Rorug03G0226400
rosa_samantha Rh3AG275900 Rh3BG311100 Rh3CG310500 Rh3DG306900
rosa_wichuraiana Rw3G024580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 401
AccI GTMKAC 1 cut(s) 160
AccII CGCG 1 cut(s) 74
AciI CCGC 1 cut(s) 168
AclWI GGATC 2 cut(s) 146, 299
AcoI YGGCCR 1 cut(s) 3
AcvI CACGTG 1 cut(s) 173
AgsI TTSAA 4 cut(s) 268, 362, 493, 523
AjiI CACGTC 2 cut(s) 187, 379
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 3 cut(s) 344, 496, 529
AluI AGCT 3 cut(s) 344, 496, 529
Alw26I GTCTC 4 cut(s) 81, 104, 280, 465
AlwI GGATC 2 cut(s) 146, 299
AoxI GGCC 1 cut(s) 3
Asp700I GAANNNNTTC 2 cut(s) 322, 488
AspS9I GGNCC 2 cut(s) 272, 446
AvaII GGWCC 2 cut(s) 272, 446
BaeGI GKGCMC 1 cut(s) 126
BaeI ACNNNNGTAYC 2 cut(s) 443, 476
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 401
BbrPI CACGTG 1 cut(s) 173
BccI CCATC 1 cut(s) 437
BcoDI GTCTC 4 cut(s) 81, 104, 280, 465
BisI GCNGC 1 cut(s) 168
BlsI GCNGC 1 cut(s) 169
Bme18I GGWCC 2 cut(s) 272, 446
BmgBI CACGTC 2 cut(s) 187, 379
BmgT120I GGNCC 2 cut(s) 272, 446
BmiI GGNNCC 1 cut(s) 403
BsaAI YACGTR 2 cut(s) 158, 173
BsaJI CCNNGG 1 cut(s) 142
Bse1I ACTGG 2 cut(s) 62, 393
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 2 cut(s) 232, 448
BseNI ACTGG 2 cut(s) 62, 393
BseRI GAGGAG 2 cut(s) 298, 453
BseSI GKGCMC 1 cut(s) 126
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 401
BslFI GGGAC 2 cut(s) 160, 389
BsmAI GTCTC 4 cut(s) 81, 104, 280, 465
BsmBI CGTCTC 2 cut(s) 280, 465
BsmFI GGGAC 2 cut(s) 160, 389
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 3 cut(s) 151, 291, 414
BspACI CCGC 1 cut(s) 168
BspANI GGCC 1 cut(s) 5
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 1 cut(s) 403
BspPI GGATC 2 cut(s) 146, 299
BspT107I GGYRCC 1 cut(s) 401
BsrI ACTGG 2 cut(s) 62, 393
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 3 cut(s) 151, 291, 414
BssNAI GTATAC 1 cut(s) 161
BssT1I CCWWGG 1 cut(s) 142
Bst1107I GTATAC 1 cut(s) 161
BstBAI YACGTR 2 cut(s) 158, 173
BstF5I GGATG 2 cut(s) 232, 448
BstFNI CGCG 1 cut(s) 74
BstKTI GATC 3 cut(s) 154, 294, 417
BstMAI GTCTC 4 cut(s) 81, 104, 280, 465
BstMBI GATC 3 cut(s) 151, 291, 414
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNSI RCATGY 1 cut(s) 28
BstSLI GKGCMC 1 cut(s) 126
BstUI CGCG 1 cut(s) 74
BstX2I RGATCY 1 cut(s) 151
BstYI RGATCY 1 cut(s) 151
BstZ17I GTATAC 1 cut(s) 161
BsuRI GGCC 1 cut(s) 5
BtrI CACGTC 2 cut(s) 187, 379
BtsCI GGATG 2 cut(s) 232, 448
BtsIMutI CAGTG 1 cut(s) 180
Cfr13I GGNCC 2 cut(s) 272, 446
CseI GACGC 1 cut(s) 239
CspCI CAANNNNNGTGG 2 cut(s) 226, 261
CviAII CATG 3 cut(s) 25, 278, 481
CviJI RGCY 6 cut(s) 5, 83, 167, 344, 496, 529
CviKI_1 RGCY 6 cut(s) 5, 83, 167, 344, 496, 529
DpnI GATC 3 cut(s) 153, 293, 416
DpnII GATC 3 cut(s) 151, 291, 414
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 142
Eco47I GGWCC 2 cut(s) 272, 446
Eco72I CACGTG 1 cut(s) 173
EcoT14I CCWWGG 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 142
Esp3I CGTCTC 2 cut(s) 280, 465
FaeI CATG 3 cut(s) 28, 281, 484
FaiI YATR 8 cut(s) 26, 103, 161, 243, 279, 433, 477, 482
FaqI GGGAC 2 cut(s) 160, 389
FatI CATG 3 cut(s) 24, 277, 480
FblI GTMKAC 1 cut(s) 160
Fnu4HI GCNGC 1 cut(s) 168
FokI GGATG 2 cut(s) 239, 455
Fsp4HI GCNGC 1 cut(s) 168
GluI GCNGC 1 cut(s) 168
HaeIII GGCC 1 cut(s) 5
HgaI GACGC 1 cut(s) 239
Hin1II CATG 3 cut(s) 28, 281, 484
HindIII AAGCTT 2 cut(s) 494, 527
HinfI GANTC 3 cut(s) 76, 252, 504
Hpy166II GTNNAC 1 cut(s) 161
Hpy188I TCNGA 1 cut(s) 450
Hpy188III TCNNGA 1 cut(s) 501
Hpy8I GTNNAC 1 cut(s) 161
Hpy99I CGWCG 2 cut(s) 43, 261
HpyAV CCTTC 2 cut(s) 136, 432
HpyCH4IV ACGT 7 cut(s) 38, 157, 172, 186, 378, 429, 458
HpyCH4V TGCA 3 cut(s) 105, 220, 303
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpySE526I ACGT 7 cut(s) 38, 157, 172, 186, 378, 429, 458
Hsp92II CATG 3 cut(s) 28, 281, 484
Kzo9I GATC 3 cut(s) 151, 291, 414
LpnPI CCDG 6 cut(s) 75, 93, 97, 177, 374, 486
MaeII ACGT 7 cut(s) 38, 157, 172, 186, 378, 429, 458
MaeIII GTNAC 2 cut(s) 173, 532
MalI GATC 3 cut(s) 153, 293, 416
MboI GATC 3 cut(s) 151, 291, 414
MboII GAAGA 1 cut(s) 503
MflI RGATCY 1 cut(s) 151
MhlI GDGCHC 1 cut(s) 126
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 195, 310, 318
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 261
MnlI CCTC 6 cut(s) 143, 188, 276, 285, 289, 474
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 2 cut(s) 322, 488
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 47, 194
MslI CAYNNNNRTG 1 cut(s) 185
Msp20I TGGCCA 1 cut(s) 5
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 1 cut(s) 80
NdeII GATC 3 cut(s) 151, 291, 414
NlaIII CATG 3 cut(s) 28, 281, 484
NlaIV GGNNCC 1 cut(s) 403
NmuCI GTSAC 2 cut(s) 173, 532
NspI RCATGY 1 cut(s) 28
OliI CACNNNNGTG 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PdmI GAANNNNTTC 2 cut(s) 322, 488
PfeI GAWTC 2 cut(s) 76, 504
PkrI GCNGC 1 cut(s) 169
PleI GAGTC 1 cut(s) 260
PmaCI CACGTG 1 cut(s) 173
PmlI CACGTG 1 cut(s) 173
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 2 cut(s) 158, 173
PspCI CACGTG 1 cut(s) 173
PspN4I GGNNCC 1 cut(s) 403
PspPI GGNCC 2 cut(s) 272, 446
PsuI RGATCY 1 cut(s) 151
RseI CAYNNNNRTG 1 cut(s) 185
SaqAI TTAA 2 cut(s) 47, 194
SatI GCNGC 1 cut(s) 168
Sau3AI GATC 3 cut(s) 151, 291, 414
Sau96I GGNCC 2 cut(s) 272, 446
SchI GAGTC 1 cut(s) 261
SduI GDGCHC 1 cut(s) 126
SinI GGWCC 2 cut(s) 272, 446
SmiMI CAYNNNNRTG 1 cut(s) 185
Sse9I AATT 3 cut(s) 195, 310, 318
SsiI CCGC 1 cut(s) 168
StyI CCWWGG 1 cut(s) 142
TaiI ACGT 7 cut(s) 41, 160, 175, 189, 381, 432, 461
TaqI TCGA 3 cut(s) 259, 294, 417
TasI AATT 3 cut(s) 195, 310, 318
TauI GCSGC 1 cut(s) 170
TfiI GAWTC 2 cut(s) 76, 504
Tru1I TTAA 2 cut(s) 47, 194
Tru9I TTAA 2 cut(s) 47, 194
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 2 cut(s) 173, 532
Tsp45I GTSAC 2 cut(s) 173, 532
TspDTI ATGAA 1 cut(s) 497
TspGWI ACGGA 3 cut(s) 245, 304, 330
TspRI CASTG 1 cut(s) 187
VpaK11BI GGWCC 2 cut(s) 272, 446
XceI RCATGY 1 cut(s) 28
XmiI GTMKAC 1 cut(s) 160
XmnI GAANNNNTTC 2 cut(s) 322, 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.