Rh3AG275900

Invertase pectin methylesterase inhibitor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0015949
Physical Location & Seq
Forward (+)
31772030 .. 31790186
18157 bp
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UTR
Exon/CDS
Intron
Rh3AG275900.1

Sequence Viewer

Length: 546 bp
ATGGCTTCTGACAACCGCAGCTGGTCGATAGTACTGTTTCTTGCTCTCCTTTTGTACTATCACTCAATCGTCGTCACTGCAGCTGATCAAGCCTTGCTTGACAAAATATGCCAGCTGGCTCAGAGTTACGATTTTTGCCTCTCAACACTGCGCAACGACCCACGTACTGGTCAAGCTGACCTTCGTGGCCTTTCCCTAATCTCCATTGCAATAACCATTGATGAAGTACGAGATGTTTCCGATCGACTGCCTAACATTCCTGTACCTAATCATCCCGTCGATCAGCAACGGAAAAAGGCTTGTCAGACTGATTATAGTGACGCATTAACCAGTTTCCAGAAGGCTTACCAGACCTCAGCTAAAGGGGATTACTGGGGTGTGATCGACTTGGTGAGGGTTGGAGCAAACAAAGCTATAGATTGCGAAGACATATACAAGAGAGATCCGATAAGCGTGTCACCAGTCTCTACAGATAACCACAAAGTTTGCTGCGTTCTATCAAGAAGCCATAACCATGACCCACATGCTGCGCTAGAACTGAACTGA

Protein Analysis

181

Amino Acids

20.16

Weight (kDa)

5.8

Isoelectric Point (pI)

35.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 145 5.6e-11 Plant invertase/pectin methylesterase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0015949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31425 AT2G31430 AT2G31432 AT2G31432 AT3G55680
rosa_chinensis RchiOBHm_Chr3g0486661
rosa_laevigata RLG00000023068
rosa_multiflora Rmu_sc0000261.1_g000009
rosa_roxburghii Rroxscaffold_6G00395680
rosa_rugosa Rorug03G0226400
rosa_samantha Rh3AG275900 Rh3BG311100 Rh3CG310500 Rh3DG306900
rosa_wichuraiana Rw3G024580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 152
AccB7I CCANNNNNTGG 1 cut(s) 167
AciI CCGC 1 cut(s) 16
AclWI GGATC 1 cut(s) 437
AfaI GTAC 5 cut(s) 33, 56, 166, 228, 264
AfiI CCNNNNNNNGG 1 cut(s) 167
AluBI AGCT 6 cut(s) 21, 83, 115, 176, 359, 413
AluI AGCT 6 cut(s) 21, 83, 115, 176, 359, 413
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 1 cut(s) 437
AoxI GGCC 1 cut(s) 187
ApeKI GCWGC 4 cut(s) 18, 80, 489, 527
AspLEI GCGC 2 cut(s) 153, 532
AsuHPI GGTGA 2 cut(s) 403, 450
BbsI GAAGAC 1 cut(s) 432
BbvCI CCTCAGC 1 cut(s) 355
BbvI GCAGC 4 cut(s) 30, 92, 476, 514
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 469
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 3 cut(s) 78, 414, 468
BisI GCNGC 4 cut(s) 19, 81, 490, 528
BlsI GCNGC 4 cut(s) 20, 82, 491, 529
BmcAI AGTACT 1 cut(s) 33
BmrI ACTGGG 1 cut(s) 382
BmuI ACTGGG 1 cut(s) 382
BpiI GAAGAC 1 cut(s) 432
Bpu10I CCTNAGC 1 cut(s) 355
BsaAI YACGTR 1 cut(s) 164
Bsc4I CCNNNNNNNGG 1 cut(s) 167
Bse1I ACTGG 4 cut(s) 172, 330, 377, 461
Bse3DI GCAATG 1 cut(s) 204
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 167
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 134, 369
BseNI ACTGG 4 cut(s) 172, 330, 377, 461
BseXI GCAGC 4 cut(s) 30, 92, 476, 514
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 1 cut(s) 189
BsiEI CGRYCG 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 167
BsmAI GTCTC 1 cut(s) 469
BsnI GGCC 1 cut(s) 189
Bsp143I GATC 5 cut(s) 85, 241, 280, 381, 442
BspACI CCGC 1 cut(s) 16
BspANI GGCC 1 cut(s) 189
BspCNI CTCAG 2 cut(s) 133, 368
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 437
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 4 cut(s) 172, 330, 377, 461
BssMI GATC 5 cut(s) 85, 241, 280, 381, 442
Bst4CI ACNGT 1 cut(s) 36
BstBAI YACGTR 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 113, 117
BstDEI CTNAG 2 cut(s) 120, 355
BstF5I GGATG 1 cut(s) 271
BstHHI GCGC 2 cut(s) 153, 532
BstKTI GATC 5 cut(s) 88, 244, 283, 384, 445
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 5 cut(s) 85, 241, 280, 381, 442
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 2 cut(s) 89, 410
BstNSI RCATGY 1 cut(s) 527
BstSFI CTRYAG 3 cut(s) 78, 414, 468
BstV1I GCAGC 4 cut(s) 30, 92, 476, 514
BstV2I GAAGAC 1 cut(s) 432
BstX2I RGATCY 1 cut(s) 442
BstYI RGATCY 1 cut(s) 442
BsuRI GGCC 1 cut(s) 189
BtsCI GGATG 1 cut(s) 271
BtsI GCAGTG 2 cut(s) 75, 146
BtsIMutI CAGTG 2 cut(s) 75, 146
Cac8I GCNNGC 2 cut(s) 113, 117
CfoI GCGC 2 cut(s) 153, 532
CseI GACGC 1 cut(s) 329
Csp6I GTAC 5 cut(s) 32, 55, 165, 227, 263
CspCI CAANNNNNGTGG 2 cut(s) 467, 502
CviAII CATG 2 cut(s) 515, 524
CviQI GTAC 5 cut(s) 32, 55, 165, 227, 263
DdeI CTNAG 2 cut(s) 120, 355
DpnI GATC 5 cut(s) 87, 243, 282, 383, 444
DpnII GATC 5 cut(s) 85, 241, 280, 381, 442
FaeI CATG 2 cut(s) 518, 527
FaiI YATR 8 cut(s) 109, 315, 416, 431, 433, 510, 516, 525
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 165, 197
FatI CATG 2 cut(s) 514, 523
FbaI TGATCA 1 cut(s) 85
Fnu4HI GCNGC 4 cut(s) 19, 81, 490, 528
FokI GGATG 1 cut(s) 258
Fsp4HI GCNGC 4 cut(s) 19, 81, 490, 528
FspBI CTAG 1 cut(s) 533
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 2 cut(s) 152, 531
GluI GCNGC 4 cut(s) 19, 81, 490, 528
HaeIII GGCC 1 cut(s) 189
HgaI GACGC 1 cut(s) 329
HhaI GCGC 2 cut(s) 153, 532
Hin1II CATG 2 cut(s) 518, 527
Hin6I GCGC 2 cut(s) 151, 530
HinP1I GCGC 2 cut(s) 151, 530
HphI GGTGA 2 cut(s) 403, 450
Hpy188I TCNGA 5 cut(s) 10, 123, 241, 306, 447
Hpy188III TCNNGA 2 cut(s) 337, 501
Hpy99I CGWCG 2 cut(s) 74, 281
HpyAV CCTTC 2 cut(s) 191, 334
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 2 cut(s) 80, 209
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 410
HpyF3I CTNAG 2 cut(s) 120, 355
HpySE526I ACGT 1 cut(s) 163
Hsp92II CATG 2 cut(s) 518, 527
HspAI GCGC 2 cut(s) 151, 530
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 5 cut(s) 85, 241, 280, 381, 442
LmnI GCTCC 1 cut(s) 401
Lsp1109I GCAGC 4 cut(s) 30, 92, 476, 514
MaeI CTAG 1 cut(s) 533
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 4 cut(s) 73, 125, 317, 456
MalI GATC 5 cut(s) 87, 243, 282, 383, 444
MboI GATC 5 cut(s) 85, 241, 280, 381, 442
MboII GAAGA 1 cut(s) 437
MflI RGATCY 1 cut(s) 442
MmeI TCCRAC 1 cut(s) 379
MnlI CCTC 3 cut(s) 149, 364, 387
MseI TTAA 1 cut(s) 326
MslI CAYNNNNRTG 1 cut(s) 513
MspA1I CMGCKG 3 cut(s) 21, 83, 115
MwoI GCNNNNNNNGC 2 cut(s) 89, 410
NdeII GATC 5 cut(s) 85, 241, 280, 381, 442
NlaIII CATG 2 cut(s) 518, 527
NmuCI GTSAC 3 cut(s) 73, 317, 456
NsbI TGCGCA 1 cut(s) 152
NspI RCATGY 1 cut(s) 527
PflMI CCANNNNNTGG 1 cut(s) 167
PkrI GCNGC 4 cut(s) 20, 82, 491, 529
Ple19I CGATCG 1 cut(s) 244
Ppu21I YACGTR 1 cut(s) 164
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 1 cut(s) 442
PvuI CGATCG 1 cut(s) 244
PvuII CAGCTG 3 cut(s) 21, 83, 115
RsaI GTAC 5 cut(s) 33, 56, 166, 228, 264
RsaNI GTAC 5 cut(s) 32, 55, 165, 227, 263
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 1 cut(s) 326
SatI GCNGC 4 cut(s) 19, 81, 490, 528
Sau3AI GATC 5 cut(s) 85, 241, 280, 381, 442
ScaI AGTACT 1 cut(s) 33
SfcI CTRYAG 3 cut(s) 78, 414, 468
SmiMI CAYNNNNRTG 1 cut(s) 513
SsiI CCGC 1 cut(s) 16
SspMI CTAG 1 cut(s) 533
TaaI ACNGT 1 cut(s) 36
TaiI ACGT 1 cut(s) 166
TaqI TCGA 4 cut(s) 26, 244, 279, 384
TatI WGTACW 2 cut(s) 31, 54
Tru1I TTAA 1 cut(s) 326
Tru9I TTAA 1 cut(s) 326
TscAI CASTG 2 cut(s) 82, 153
TseFI GTSAC 3 cut(s) 73, 317, 456
TseI GCWGC 4 cut(s) 18, 80, 489, 527
Tsp45I GTSAC 3 cut(s) 73, 317, 456
TspDTI ATGAA 1 cut(s) 237
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 2 cut(s) 82, 153
Van91I CCANNNNNTGG 1 cut(s) 167
XceI RCATGY 1 cut(s) 527
XspI CTAG 1 cut(s) 533
ZrmI AGTACT 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.