Rh3BG311100

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0015949
Physical Location & Seq
Forward (+)
31554013 .. 31565521
11509 bp
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UTR
Exon/CDS
Intron
Rh3BG311100.1

Sequence Viewer

Length: 1398 bp
ATGGCTTCTGACAACCGTAGCTGGTCGATAGTACTGTTTCTTGCTCTCGTTTTGTACTATCACTCAATCGTCGTCACTGCAGCTGATCAAGCCTTGCTTGACAAAATCTGCAAGCTGGCTCAGAGTTACGATTTTTGCCTCTCAACACTGCGCAACGACCCACGTACTGCTCAAGCTGACCTTCGTGGCCTTTCCCTAATCTCCATTGCAATAACCATTGATGAAGTACGAGATGTTTCCGATCGACTGCCTAACATTCCTGTACCTAATCATCCCGTCGATCAGCAACGGAAAAAAGCTTGTCAGACTGATTATAGTGACGCATTAGCCAGTTTCCAGAAGGCTTACCAGACCTCAGCTAAAGGGGATTACTGGGGTGTGATCGACTTGGTGCTATGGATTCATCAAGAAAAGTCAAGTCCCAGTACCCAATTCAAGATCCGCTCTTTCCAAGACAGCATAGTCGAGAAAATTAATAGGATTTATAGTCTTGTTTCTTACTGGAGGGCCAACTGGGGCATTGATTGTTGCTCCTACGAGGATGAAGCAGCCTGCCATTGTATTGATGATCTGGAGAAAATGAAAAGGATTTATGAGAGAGCGGTTGGGATTTATGCCTCACTTGTCAACATTAATGCTTACCATCAACCTGGAAGTTGTTCCACCTTGCTTCACATGCTCAAAGATGATAGGCTGGTGCAGCATCAACCTACGCAATATCCCACATCCGAGGAGTTGAGTATTGGCAAAATCAAGTTCAAGGCCTTTGATTTGGGAGGTCATCAGATTGCTCGCAGGGTCTGGAAGGATTACTACGCAAAGGTTTGCTTCACTCATGACTTTTTGAACTCCATAACAAAAGTTAAAGACATTGTGATTCTTTGCGAAGTGCTTGCGTTATTAACCAAGCTTTTGAGTGGATGGCGCCGACCTCTTGAGGACAAAGGAACTTTAGTGTATGATGGGACTTCTGCTCAACTGTTAGAGAAGTATAAAATCAAAGGGAACTTGAATCTCATTTGGACTCAAAGCGTCTTGACATTGTTTTTGGGAATTTTGATAGTGGACAAAATGAACCGCTGCCGTCACAAATGCATTGACAGGATACTATTGTGGTCTTTCTTTGATTTGACTGTAATTCATGATGACCACAAAGATAGTACAGTAACAGTGCTACAATCTGATTTTTATCGTGCCAATGAGTTGTTAGTACTAACAGATAAGCAAATAGTTGCCAAGGTGACATCTTACCTGTCAAAGTGCATCAAGGACTTCGAGAATGCTGCTGTGATAAATAAGGACAAGTTTTATGTTTTCCGGCTTTATGTATTTATGCACTTAGGGAATGTTAATTCCTATGTAAATGTTGAGATGAATATTGCTGATGGAATGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

465

Amino Acids

53.38

Weight (kDa)

8.11

Isoelectric Point (pI)

34.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 32 - 120 2.8e-10 Plant invertase/pectin methylesterase inhibitor
Arf PF00025 221 - 275 8.8e-16 ADP-ribosylation factor family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015949)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G31425 AT2G31430 AT2G31432 AT2G31432 AT3G55680
rosa_chinensis RchiOBHm_Chr3g0486661
rosa_laevigata RLG00000023068
rosa_multiflora Rmu_sc0000261.1_g000009
rosa_roxburghii Rroxscaffold_6G00395680
rosa_rugosa Rorug03G0226400
rosa_samantha Rh3AG275900 Rh3BG311100 Rh3CG310500 Rh3DG306900
rosa_wichuraiana Rw3G024580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
Acc16I TGCGCA 1 cut(s) 152
AccB1I GGYRCC 1 cut(s) 924
AccBSI CCGCTC 2 cut(s) 444, 602
AciI CCGC 3 cut(s) 442, 602, 1078
AclWI GGATC 1 cut(s) 433
AcsI RAATTY 1 cut(s) 1053
AcyI GRCGYC 1 cut(s) 925
AfaI GTAC 8 cut(s) 33, 56, 166, 228, 264, 427, 1162, 1212
AgsI TTSAA 4 cut(s) 436, 760, 847, 1012
AjnI CCWGG 1 cut(s) 649
AluBI AGCT 7 cut(s) 21, 83, 115, 176, 299, 359, 910
AluI AGCT 7 cut(s) 21, 83, 115, 176, 299, 359, 910
AlwI GGATC 1 cut(s) 433
AlwNI CAGNNNCTG 1 cut(s) 801
AoxI GGCC 3 cut(s) 187, 507, 762
ApeKI GCWGC 5 cut(s) 80, 548, 700, 1080, 1283
ApoI RAATTY 1 cut(s) 1053
ArsI GACNNNNNNTTYG 2 cut(s) 1030, 1062
AseI ATTAAT 2 cut(s) 474, 633
Asp700I GAANNNNTTC 1 cut(s) 658
AspLEI GCGC 2 cut(s) 153, 927
AspS9I GGNCC 1 cut(s) 507
AsuHPI GGTGA 1 cut(s) 1252
BaeI ACNNNNGTAYC 2 cut(s) 1097, 1130
BanI GGYRCC 1 cut(s) 924
BarI GAAGNNNNNNTAC 2 cut(s) 797, 829
BbvCI CCTCAGC 1 cut(s) 355
BbvI GCAGC 5 cut(s) 92, 560, 712, 1067, 1270
BccI CCATC 4 cut(s) 651, 915, 956, 1379
BceAI ACGGC 1 cut(s) 1068
BcgI CGANNNNNNTGC 4 cut(s) 875, 909, 1265, 1299
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 1098
BclI TGATCA 1 cut(s) 85
BfmI CTRYAG 1 cut(s) 78
BfoI RGCGCY 1 cut(s) 928
BfuI GTATCC 1 cut(s) 1098
BisI GCNGC 5 cut(s) 81, 549, 701, 1081, 1284
BlsI GCNGC 5 cut(s) 82, 550, 702, 1082, 1285
BmcAI AGTACT 2 cut(s) 33, 1212
Bme1390I CCNGG 1 cut(s) 651
BmgT120I GGNCC 1 cut(s) 507
BmiI GGNNCC 1 cut(s) 926
BmrFI CCNGG 1 cut(s) 651
BmrI ACTGGG 3 cut(s) 382, 417, 523
BmsI GCATC 2 cut(s) 712, 1272
BmuI ACTGGG 3 cut(s) 382, 417, 523
BpmI CTGGAG 2 cut(s) 523, 593
Bpu10I CCTNAGC 1 cut(s) 355
BpuEI CTTGAG 2 cut(s) 156, 956
BsaAI YACGTR 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 1188
BsaHI GRCGYC 1 cut(s) 925
BsaJI CCNNGG 2 cut(s) 729, 1236
Bse1I ACTGG 5 cut(s) 330, 377, 423, 506, 518
Bse3DI GCAATG 1 cut(s) 204
Bse8I GATNNNNATC 1 cut(s) 1188
BseBI CCWGG 1 cut(s) 651
BseDI CCNNGG 2 cut(s) 729, 1236
BseGI GGATG 4 cut(s) 271, 547, 725, 926
BseJI GATNNNNATC 1 cut(s) 1188
BseMI GCAATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 134, 369
BseNI ACTGG 5 cut(s) 330, 377, 423, 506, 518
BseRI GAGGAG 1 cut(s) 746
BseXI GCAGC 5 cut(s) 92, 560, 712, 1067, 1270
BsgI GTGCAG 1 cut(s) 719
Bsh1285I CGRYCG 1 cut(s) 244
BshFI GGCC 3 cut(s) 189, 509, 764
BshNI GGYRCC 1 cut(s) 924
BsiEI CGRYCG 1 cut(s) 244
BsiSI CCGG 1 cut(s) 1318
BslFI GGGAC 2 cut(s) 405, 979
BsmFI GGGAC 2 cut(s) 405, 979
BsmI GAATGC 1 cut(s) 1285
BsnI GGCC 3 cut(s) 189, 509, 764
Bsp143I GATC 6 cut(s) 85, 241, 280, 381, 438, 568
BspACI CCGC 3 cut(s) 442, 602, 1078
BspANI GGCC 3 cut(s) 189, 509, 764
BspCNI CTCAG 2 cut(s) 133, 368
BspHI TCATGA 2 cut(s) 835, 1141
BspLI GGNNCC 1 cut(s) 926
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 1 cut(s) 433
BspT107I GGYRCC 1 cut(s) 924
BsrBI CCGCTC 2 cut(s) 444, 602
BsrDI GCAATG 1 cut(s) 204
BsrI ACTGG 5 cut(s) 330, 377, 423, 506, 518
BssECI CCNNGG 2 cut(s) 729, 1236
BssMI GATC 6 cut(s) 85, 241, 280, 381, 438, 568
BssNI GRCGYC 1 cut(s) 925
BssT1I CCWWGG 1 cut(s) 1236
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 6 cut(s) 17, 36, 981, 1135, 1165, 1171
BstACI GRCGYC 1 cut(s) 925
BstBAI YACGTR 1 cut(s) 164
BstC8I GCNNGC 5 cut(s) 113, 117, 553, 793, 894
BstDEI CTNAG 3 cut(s) 120, 355, 1339
BstF5I GGATG 4 cut(s) 271, 547, 725, 926
BstH2I RGCGCY 1 cut(s) 928
BstHHI GCGC 2 cut(s) 153, 927
BstKTI GATC 6 cut(s) 88, 244, 283, 384, 441, 571
BstMBI GATC 6 cut(s) 85, 241, 280, 381, 438, 568
BstMCI CGRYCG 1 cut(s) 244
BstMWI GCNNNNNNNGC 3 cut(s) 89, 676, 700
BstNI CCWGG 1 cut(s) 651
BstNSI RCATGY 1 cut(s) 679
BstSCI CCNGG 1 cut(s) 649
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 5 cut(s) 92, 560, 712, 1067, 1270
BstX2I RGATCY 1 cut(s) 438
BstXI CCANNNNNNTGG 1 cut(s) 650
BstYI RGATCY 1 cut(s) 438
BsuI GTATCC 1 cut(s) 1098
BsuRI GGCC 3 cut(s) 189, 509, 764
BtsCI GGATG 4 cut(s) 271, 547, 725, 926
BtsI GCAGTG 2 cut(s) 75, 146
BtsIMutI CAGTG 3 cut(s) 75, 146, 1176
Cac8I GCNNGC 5 cut(s) 113, 117, 553, 793, 894
CaiI CAGNNNCTG 1 cut(s) 801
CciI TCATGA 2 cut(s) 835, 1141
CfoI GCGC 2 cut(s) 153, 927
Cfr13I GGNCC 1 cut(s) 507
CseI GACGC 2 cut(s) 329, 1021
Csp6I GTAC 8 cut(s) 32, 55, 165, 227, 263, 426, 1161, 1211
CviAII CATG 3 cut(s) 676, 836, 1142
CviQI GTAC 8 cut(s) 32, 55, 165, 227, 263, 426, 1161, 1211
DdeI CTNAG 3 cut(s) 120, 355, 1339
DinI GGCGCC 1 cut(s) 926
DpnI GATC 6 cut(s) 87, 243, 282, 383, 440, 570
DpnII GATC 6 cut(s) 85, 241, 280, 381, 438, 568
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
Eco130I CCWWGG 1 cut(s) 1236
Eco147I AGGCCT 1 cut(s) 764
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 1236
EcoT22I ATGCAT 1 cut(s) 1097
EgeI GGCGCC 1 cut(s) 926
EheI GGCGCC 1 cut(s) 926
ErhI CCWWGG 1 cut(s) 1236
FaeI CATG 3 cut(s) 679, 839, 1145
FalI AAGNNNNNCTT 6 cut(s) 81, 113, 165, 197, 812, 844
FaqI GGGAC 2 cut(s) 405, 979
FatI CATG 3 cut(s) 675, 835, 1141
FbaI TGATCA 1 cut(s) 85
Fnu4HI GCNGC 5 cut(s) 81, 549, 701, 1081, 1284
FokI GGATG 4 cut(s) 258, 554, 712, 933
Fsp4HI GCNGC 5 cut(s) 81, 549, 701, 1081, 1284
FspI TGCGCA 1 cut(s) 152
GlaI GCGC 2 cut(s) 152, 926
GluI GCNGC 5 cut(s) 81, 549, 701, 1081, 1284
GsuI CTGGAG 2 cut(s) 523, 593
HaeII RGCGCY 1 cut(s) 928
HaeIII GGCC 3 cut(s) 189, 509, 764
HapII CCGG 1 cut(s) 1318
HgaI GACGC 2 cut(s) 329, 1021
HhaI GCGC 2 cut(s) 153, 927
Hin1I GRCGYC 1 cut(s) 925
Hin1II CATG 3 cut(s) 679, 839, 1145
Hin6I GCGC 2 cut(s) 151, 925
HinP1I GCGC 2 cut(s) 151, 925
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HindIII AAGCTT 2 cut(s) 297, 908
HinfI GANTC 4 cut(s) 400, 877, 1012, 1024
HpaII CCGG 1 cut(s) 1318
HphI GGTGA 1 cut(s) 1252
Hpy166II GTNNAC 2 cut(s) 628, 1066
Hpy188I TCNGA 7 cut(s) 10, 123, 241, 306, 730, 786, 1183
Hpy8I GTNNAC 2 cut(s) 628, 1066
Hpy99I CGWCG 2 cut(s) 74, 281
HpyAV CCTTC 3 cut(s) 191, 334, 799
HpyCH4III ACNGT 6 cut(s) 17, 36, 981, 1135, 1165, 1171
HpyCH4IV ACGT 1 cut(s) 163
HpyCH4V TGCA 7 cut(s) 80, 111, 209, 700, 1095, 1263, 1336
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 676, 700
HpyF3I CTNAG 3 cut(s) 120, 355, 1339
HpySE526I ACGT 1 cut(s) 163
Hsp92I GRCGYC 1 cut(s) 925
Hsp92II CATG 3 cut(s) 679, 839, 1145
HspAI GCGC 2 cut(s) 151, 925
KasI GGCGCC 1 cut(s) 924
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 6 cut(s) 85, 241, 280, 381, 438, 568
LmnI GCTCC 1 cut(s) 536
Lsp1109I GCAGC 5 cut(s) 92, 560, 712, 1067, 1270
LweI GCATC 2 cut(s) 712, 1272
MaeII ACGT 1 cut(s) 163
MaeIII GTNAC 6 cut(s) 73, 125, 317, 1085, 1165, 1240
MalI GATC 6 cut(s) 87, 243, 282, 383, 440, 570
MbiI CCGCTC 2 cut(s) 444, 602
MboI GATC 6 cut(s) 85, 241, 280, 381, 438, 568
MflI RGATCY 1 cut(s) 438
MluCI AATT 5 cut(s) 431, 471, 1053, 1137, 1351
Mly113I GGCGCC 1 cut(s) 925
MlyI GAGTC 1 cut(s) 1018
MnlI CCTC 9 cut(s) 149, 364, 498, 532, 628, 724, 770, 931, 942
Mph1103I ATGCAT 1 cut(s) 1097
MroXI GAANNNNTTC 1 cut(s) 658
MseI TTAA 5 cut(s) 474, 633, 864, 902, 1350
MspA1I CMGCKG 2 cut(s) 83, 1080
MspI CCGG 1 cut(s) 1318
MspR9I CCNGG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 1285
MvaI CCWGG 1 cut(s) 651
MwoI GCNNNNNNNGC 3 cut(s) 89, 676, 700
NarI GGCGCC 1 cut(s) 925
NdeII GATC 6 cut(s) 85, 241, 280, 381, 438, 568
NlaIII CATG 3 cut(s) 679, 839, 1145
NlaIV GGNNCC 1 cut(s) 926
NmuCI GTSAC 4 cut(s) 73, 317, 1085, 1240
NsbI TGCGCA 1 cut(s) 152
NsiI ATGCAT 1 cut(s) 1097
NspI RCATGY 1 cut(s) 679
PagI TCATGA 2 cut(s) 835, 1141
PceI AGGCCT 1 cut(s) 764
PctI GAATGC 1 cut(s) 1285
PdmI GAANNNNTTC 1 cut(s) 658
PfeI GAWTC 3 cut(s) 400, 877, 1012
PkrI GCNGC 5 cut(s) 82, 550, 702, 1082, 1285
Ple19I CGATCG 1 cut(s) 244
PleI GAGTC 1 cut(s) 1018
PluTI GGCGCC 1 cut(s) 928
PpsI GAGTC 1 cut(s) 1018
Ppu21I YACGTR 1 cut(s) 164
PshBI ATTAAT 2 cut(s) 474, 633
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 1 cut(s) 926
PspPI GGNCC 1 cut(s) 507
PstI CTGCAG 1 cut(s) 82
PstNI CAGNNNCTG 1 cut(s) 801
PsuI RGATCY 1 cut(s) 438
PvuI CGATCG 1 cut(s) 244
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 8 cut(s) 33, 56, 166, 228, 264, 427, 1162, 1212
RsaNI GTAC 8 cut(s) 32, 55, 165, 227, 263, 426, 1161, 1211
SaqAI TTAA 5 cut(s) 474, 633, 864, 902, 1350
SatI GCNGC 5 cut(s) 81, 549, 701, 1081, 1284
Sau3AI GATC 6 cut(s) 85, 241, 280, 381, 438, 568
Sau96I GGNCC 1 cut(s) 507
ScaI AGTACT 2 cut(s) 33, 1212
SchI GAGTC 1 cut(s) 1018
ScrFI CCNGG 1 cut(s) 651
SfaNI GCATC 2 cut(s) 712, 1272
SfcI CTRYAG 1 cut(s) 78
SfoI GGCGCC 1 cut(s) 926
SmlI CTYRAG 2 cut(s) 171, 935
SmoI CTYRAG 2 cut(s) 171, 935
Sse9I AATT 5 cut(s) 431, 471, 1053, 1137, 1351
SseBI AGGCCT 1 cut(s) 764
SsiI CCGC 3 cut(s) 442, 602, 1078
SspDI GGCGCC 1 cut(s) 924
SspI AATATT 1 cut(s) 1378
StuI AGGCCT 1 cut(s) 764
StyD4I CCNGG 1 cut(s) 649
StyI CCWWGG 1 cut(s) 1236
TaaI ACNGT 6 cut(s) 17, 36, 981, 1135, 1165, 1171
TaiI ACGT 1 cut(s) 166
TaqI TCGA 6 cut(s) 26, 244, 279, 384, 465, 1275
TasI AATT 5 cut(s) 431, 471, 1053, 1137, 1351
TatI WGTACW 4 cut(s) 31, 54, 1160, 1210
TfiI GAWTC 3 cut(s) 400, 877, 1012
Tru1I TTAA 5 cut(s) 474, 633, 864, 902, 1350
Tru9I TTAA 5 cut(s) 474, 633, 864, 902, 1350
TscAI CASTG 3 cut(s) 82, 153, 1176
TseFI GTSAC 4 cut(s) 73, 317, 1085, 1240
TseI GCWGC 5 cut(s) 80, 548, 700, 1080, 1283
Tsp45I GTSAC 4 cut(s) 73, 317, 1085, 1240
TspDTI ATGAA 7 cut(s) 237, 392, 558, 596, 1088, 1130, 1388
TspGWI ACGGA 1 cut(s) 304
TspRI CASTG 3 cut(s) 82, 153, 1176
VspI ATTAAT 2 cut(s) 474, 633
XapI RAATTY 1 cut(s) 1053
XceI RCATGY 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 658
ZrmI AGTACT 2 cut(s) 33, 1212
Zsp2I ATGCAT 1 cut(s) 1097
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.