AT2G32720

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0007036
Physical Location & Seq
Reverse (-)
13875812 .. 13878710
2899 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32720.2

Sequence Viewer

Length: 405 bp
ATGGGAGACGAAGCAAAGATCTTCACTCTTTCAGAAGTTTCAGAGCATAATCAAGCTCATGACTGTTGGATTGTCATCAATGGAAAGGTGTACAATGTGACCAAGTTTCTTGAAGACCATCCAGGTGGGGACGATGTTCTCTTGTCTTCAACAGGTAAGGATGCAACGGATGATTTTGAGGACGTGGGTCACAGCGAGAGTGCAAGAGAAATGATGGAGCAGTACTACGTAGGAGAGATTGATCCAACAACAATACCAAAGAAAGTCAAATACACACCTCCTAAACAGCCTCACTACAATCAAGACAAGACCTCTGAATTCATAATCAAGCTCCTCCAGTTCCTCGTACCCCTTGCCATTCTTGGTTTAGCAGTCGGAATCCGTATCTACACCAAATCAGGGTAG

Protein Analysis

134

Amino Acids

15.02

Weight (kDa)

4.98

Isoelectric Point (pI)

26.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 21 - 80 2.9e-22 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 236
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 3 cut(s) 92, 224, 348
AfiI CCNNNNNNNGG 1 cut(s) 399
AgsI TTSAA 2 cut(s) 113, 150
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 1 cut(s) 121
AluBI AGCT 2 cut(s) 56, 331
AluI AGCT 2 cut(s) 56, 331
AlwI GGATC 1 cut(s) 236
ApoI RAATTY 1 cut(s) 317
BbsI GAAGAC 2 cut(s) 120, 138
BccI CCATC 2 cut(s) 126, 208
BciT130I CCWGG 1 cut(s) 123
BglII AGATCT 1 cut(s) 18
BmcAI AGTACT 1 cut(s) 224
Bme1390I CCNGG 1 cut(s) 123
BmgBI CACGTC 1 cut(s) 184
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 1 cut(s) 151
BoxI GACNNNNGTC 1 cut(s) 186
BpiI GAAGAC 2 cut(s) 120, 138
BpmI CTGGAG 1 cut(s) 320
BsaAI YACGTR 1 cut(s) 229
BsaBI GATNNNNATC 1 cut(s) 74
Bsc4I CCNNNNNNNGG 1 cut(s) 399
Bse1I ACTGG 1 cut(s) 337
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 123
BseGI GGATG 3 cut(s) 118, 166, 175
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 1 cut(s) 399
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 323
BslFI GGGAC 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 399
BsmFI GGGAC 1 cut(s) 143
Bsp1407I TGTACA 1 cut(s) 90
Bsp143I GATC 2 cut(s) 18, 241
BspHI TCATGA 1 cut(s) 58
BspPI GGATC 1 cut(s) 236
BsrGI TGTACA 1 cut(s) 90
BsrI ACTGG 1 cut(s) 337
BssMI GATC 2 cut(s) 18, 241
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 65
BstAUI TGTACA 1 cut(s) 90
BstBAI YACGTR 1 cut(s) 229
BstF5I GGATG 3 cut(s) 118, 166, 175
BstKTI GATC 2 cut(s) 21, 244
BstMBI GATC 2 cut(s) 18, 241
BstNI CCWGG 1 cut(s) 123
BstPAI GACNNNNGTC 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 121
BstSNI TACGTA 1 cut(s) 229
BstV2I GAAGAC 2 cut(s) 120, 138
BstX2I RGATCY 1 cut(s) 18
BstXI CCANNNNNNTGG 1 cut(s) 125
BstYI RGATCY 1 cut(s) 18
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 3 cut(s) 118, 166, 175
CciI TCATGA 1 cut(s) 58
Csp6I GTAC 3 cut(s) 91, 223, 347
CviAII CATG 1 cut(s) 59
CviJI RGCY 3 cut(s) 56, 289, 331
CviKI_1 RGCY 3 cut(s) 56, 289, 331
CviQI GTAC 3 cut(s) 91, 223, 347
DpnI GATC 2 cut(s) 20, 243
DpnII GATC 2 cut(s) 18, 241
Eco105I TACGTA 1 cut(s) 229
EcoRI GAATTC 1 cut(s) 317
EcoRII CCWGG 1 cut(s) 121
FaeI CATG 1 cut(s) 62
FaiI YATR 3 cut(s) 48, 60, 323
FaqI GGGAC 1 cut(s) 143
FatI CATG 1 cut(s) 58
FokI GGATG 3 cut(s) 105, 173, 182
GsuI CTGGAG 1 cut(s) 320
Hin1II CATG 1 cut(s) 62
HinfI GANTC 1 cut(s) 378
Hpy166II GTNNAC 1 cut(s) 91
Hpy188I TCNGA 4 cut(s) 34, 43, 316, 377
Hpy188III TCNNGA 3 cut(s) 59, 110, 302
Hpy8I GTNNAC 1 cut(s) 91
HpyCH4III ACNGT 1 cut(s) 65
HpyCH4IV ACGT 2 cut(s) 183, 228
HpyCH4V TGCA 2 cut(s) 164, 203
HpySE526I ACGT 2 cut(s) 183, 228
Hsp92II CATG 1 cut(s) 62
Kzo9I GATC 2 cut(s) 18, 241
LmnI GCTCC 2 cut(s) 217, 336
LpnPI CCDG 5 cut(s) 108, 135, 138, 350, 384
LweI GCATC 1 cut(s) 151
MaeII ACGT 2 cut(s) 183, 228
MaeIII GTNAC 2 cut(s) 97, 188
MalI GATC 2 cut(s) 20, 243
MboI GATC 2 cut(s) 18, 241
MboII GAAGA 3 cut(s) 13, 125, 138
MflI RGATCY 1 cut(s) 18
MluCI AATT 1 cut(s) 317
MmeI TCCRAC 3 cut(s) 47, 269, 355
MnlI CCTC 6 cut(s) 172, 288, 300, 322, 344, 353
MslI CAYNNNNRTG 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
NdeII GATC 2 cut(s) 18, 241
NlaIII CATG 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 97, 188
PagI TCATGA 1 cut(s) 58
PfeI GAWTC 1 cut(s) 378
Ppu21I YACGTR 1 cut(s) 229
PshAI GACNNNNGTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PsuI RGATCY 1 cut(s) 18
RsaI GTAC 3 cut(s) 92, 224, 348
RsaNI GTAC 3 cut(s) 91, 223, 347
RseI CAYNNNNRTG 1 cut(s) 123
Sau3AI GATC 2 cut(s) 18, 241
ScaI AGTACT 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 123
SetI ASST 9 cut(s) 58, 90, 127, 157, 186, 231, 280, 314, 333
SfaNI GCATC 1 cut(s) 151
SmiMI CAYNNNNRTG 1 cut(s) 123
SnaBI TACGTA 1 cut(s) 229
Sse9I AATT 1 cut(s) 317
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 65
TaiI ACGT 2 cut(s) 186, 231
TasI AATT 1 cut(s) 317
TatI WGTACW 2 cut(s) 90, 222
TfiI GAWTC 1 cut(s) 378
TseFI GTSAC 2 cut(s) 97, 188
Tsp45I GTSAC 2 cut(s) 97, 188
TspDTI ATGAA 1 cut(s) 310
TspGWI ACGGA 2 cut(s) 182, 371
XapI RAATTY 1 cut(s) 317
ZrmI AGTACT 1 cut(s) 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.