FvH4_4g00670

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0007036
Physical Location & Seq
Reverse (-)
581636 .. 584757
3122 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g00670.t1

Sequence Viewer

Length: 405 bp
ATGGGTGGCGATGAAAAGGTCTTCACTTTGGCTCAGGTCTCTGACCACAACACCCCAAAGGACTGCTGGCTAATCATCAATGGCAAGGTTTATGATGTGACCAAGTTCTTGGAGGACCATCCTGGTGGTGATGATGTTTTGTTGTCAGCAACAGGGAAGGATGCAACTGATGATTTTGAAGACGTGGGTCACAGTGATAATGCCAGAGACATGTTGAAGGACTTCTATGTGGGAGAGATTGATGTCTCTACCATCCCCAAGAAGACTGCATACACTCCTCCCAAACAGCCTCATTACAATCAAGACAAGACTTCAGAGTTCATCATCAAACTTCTCCAGTTCCTTGTTCCCATTGCCATATTGGGGTTGGCTTTTGGCATCCGCCTTTACACCAAGTCATCTTAA

Protein Analysis

135

Amino Acids

14.91

Weight (kDa)

4.9

Isoelectric Point (pI)

26.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 80 3.2e-31 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 382
AcuI CTGAAG 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 363
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 2 cut(s) 179, 217
AjiI CACGTC 1 cut(s) 184
AjnI CCWGG 1 cut(s) 121
Alw26I GTCTC 3 cut(s) 43, 201, 250
Asp700I GAANNNNTTC 1 cut(s) 221
AspS9I GGNCC 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 140
AvaII GGWCC 1 cut(s) 115
BarI GAAGNNNNNNTAC 2 cut(s) 254, 286
BbsI GAAGAC 3 cut(s) 13, 186, 269
BccI CCATC 2 cut(s) 126, 260
BciT130I CCWGG 1 cut(s) 123
BcoDI GTCTC 3 cut(s) 43, 201, 250
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 115
BmgBI CACGTC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 115
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 2 cut(s) 151, 387
BoxI GACNNNNGTC 1 cut(s) 186
BpiI GAAGAC 3 cut(s) 13, 186, 269
BpmI CTGGAG 1 cut(s) 320
Bpu10I CCTNAGC 1 cut(s) 33
BsaI GGTCTC 1 cut(s) 43
Bsc4I CCNNNNNNNGG 1 cut(s) 363
Bse1I ACTGG 1 cut(s) 337
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 1 cut(s) 123
BseGI GGATG 4 cut(s) 118, 166, 252, 378
BseLI CCNNNNNNNGG 1 cut(s) 363
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 1 cut(s) 267
BslI CCNNNNNNNGG 1 cut(s) 363
BsmAI GTCTC 3 cut(s) 43, 201, 250
Bso31I GGTCTC 1 cut(s) 43
BspACI CCGC 1 cut(s) 382
BspCNI CTCAG 1 cut(s) 46
BspTNI GGTCTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 1 cut(s) 337
Bst2UI CCWGG 1 cut(s) 123
Bst4CI ACNGT 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 68
BstDEI CTNAG 1 cut(s) 33
BstF5I GGATG 4 cut(s) 118, 166, 252, 378
BstMAI GTCTC 3 cut(s) 43, 201, 250
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 1 cut(s) 214
BstPAI GACNNNNGTC 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 121
BstV2I GAAGAC 3 cut(s) 13, 186, 269
BstXI CCANNNNNNTGG 2 cut(s) 109, 125
BtgZI GCGATG 1 cut(s) 24
BtrI CACGTC 1 cut(s) 184
BtsCI GGATG 4 cut(s) 118, 166, 252, 378
BtsIMutI CAGTG 1 cut(s) 199
Cac8I GCNNGC 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 115
CviAII CATG 1 cut(s) 211
CviJI RGCY 4 cut(s) 32, 70, 289, 371
CviKI_1 RGCY 4 cut(s) 32, 70, 289, 371
DdeI CTNAG 1 cut(s) 33
EciI GGCGGA 1 cut(s) 371
Eco31I GGTCTC 1 cut(s) 43
Eco47I GGWCC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 297
EcoRII CCWGG 1 cut(s) 121
FaeI CATG 1 cut(s) 214
FaiI YATR 5 cut(s) 93, 212, 228, 271, 359
FatI CATG 1 cut(s) 210
FokI GGATG 4 cut(s) 105, 173, 239, 365
GsuI CTGGAG 1 cut(s) 320
Hin1II CATG 1 cut(s) 214
HphI GGTGA 1 cut(s) 140
Hpy188I TCNGA 2 cut(s) 43, 316
Hpy188III TCNNGA 1 cut(s) 302
HpyAV CCTTC 2 cut(s) 151, 211
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 2 cut(s) 164, 269
HpyF3I CTNAG 1 cut(s) 33
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 1 cut(s) 214
LpnPI CCDG 7 cut(s) 20, 52, 108, 135, 138, 217, 350
LweI GCATC 2 cut(s) 151, 387
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 2 cut(s) 97, 188
MboII GAAGA 3 cut(s) 13, 191, 274
MnlI CCTC 3 cut(s) 106, 288, 300
MroXI GAANNNNTTC 1 cut(s) 221
MseI TTAA 1 cut(s) 403
MslI CAYNNNNRTG 1 cut(s) 123
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
NlaIII CATG 1 cut(s) 214
NmuCI GTSAC 2 cut(s) 97, 188
NspI RCATGY 1 cut(s) 214
PciI ACATGT 1 cut(s) 210
PdmI GAANNNNTTC 1 cut(s) 221
PscI ACATGT 1 cut(s) 210
PshAI GACNNNNGTC 1 cut(s) 186
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspPI GGNCC 1 cut(s) 115
RseI CAYNNNNRTG 1 cut(s) 123
SaqAI TTAA 1 cut(s) 403
Sau96I GGNCC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 123
SetI ASST 4 cut(s) 21, 39, 90, 186
SfaNI GCATC 2 cut(s) 151, 387
SinI GGWCC 1 cut(s) 115
SmiMI CAYNNNNRTG 1 cut(s) 123
SsiI CCGC 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 186
Tru1I TTAA 1 cut(s) 403
Tru9I TTAA 1 cut(s) 403
TscAI CASTG 1 cut(s) 199
TseFI GTSAC 2 cut(s) 97, 188
Tsp45I GTSAC 2 cut(s) 97, 188
TspDTI ATGAA 2 cut(s) 27, 310
TspRI CASTG 1 cut(s) 199
VpaK11BI GGWCC 1 cut(s) 115
XceI RCATGY 1 cut(s) 214
XcmI CCANNNNNNNNNTGG 3 cut(s) 63, 358, 364
XmnI GAANNNNTTC 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.