Rh4DG008500

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0007036
Physical Location & Seq
Reverse (-)
1362927 .. 1367485
4559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG008500.1

Sequence Viewer

Length: 351 bp
ATGTTCTTATTGCCAAATTATTTCTTGGATCAGGTTTATGATGTGACCAAGTTCTTGGATGACCATCCTGGTGGTGATGATGTTTTGTTGTCAGCAACAGGGAAGGATGCAACTGATGATTTCGAAGACGTGGGTCACAGTGATAATGCCAGAGACATGTTGAAGGACTTCTATGTGGGAGAGATCGATGCCTCTACTATCCCCAAGAAGACCACATACACTCCTCCCAAGCAGCCTCAGTACAATCAAGACAAGACTTCAGAGTTCATCATCAAACTTCTCCAGTTCCTTGTTCCCATTGCCATATTGGGTTTGGCTTTTGGCATCCGCCTTTACGCCAAGTCATCTTAA

Protein Analysis

116

Amino Acids

13.04

Weight (kDa)

4.54

Isoelectric Point (pI)

27.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 10 - 62 5.2e-21 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 328
AclWI GGATC 1 cut(s) 36
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 1 cut(s) 163
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 232
Asp700I GAANNNNTTC 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 86
AsuII TTCGAA 1 cut(s) 123
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
BbsI GAAGAC 2 cut(s) 132, 215
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 147
BisI GCNGC 1 cut(s) 233
BlsI GCNGC 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 69
BmgBI CACGTC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 69
BmsI GCATC 3 cut(s) 97, 178, 333
BoxI GACNNNNGTC 1 cut(s) 132
BpiI GAAGAC 2 cut(s) 132, 215
BpmI CTGGAG 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 123
Bsa29I ATCGAT 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 63
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bse1I ACTGG 1 cut(s) 283
Bse3DI GCAATG 1 cut(s) 297
Bse8I GATNNNNATC 1 cut(s) 63
BseBI CCWGG 1 cut(s) 69
BseCI ATCGAT 1 cut(s) 186
BseGI GGATG 4 cut(s) 64, 64, 112, 324
BseJI GATNNNNATC 1 cut(s) 63
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 1 cut(s) 244
BshVI ATCGAT 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 123
Bsp143I GATC 2 cut(s) 28, 183
BspACI CCGC 1 cut(s) 328
BspCNI CTCAG 1 cut(s) 250
BspDI ATCGAT 1 cut(s) 186
BspPI GGATC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 1 cut(s) 283
BssMI GATC 2 cut(s) 28, 183
Bst2UI CCWGG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 123
BstDEI CTNAG 1 cut(s) 237
BstF5I GGATG 4 cut(s) 64, 64, 112, 324
BstKTI GATC 2 cut(s) 31, 186
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 2 cut(s) 28, 183
BstNI CCWGG 1 cut(s) 69
BstNSI RCATGY 1 cut(s) 160
BstPAI GACNNNNGTC 1 cut(s) 132
BstSCI CCNGG 1 cut(s) 67
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 2 cut(s) 132, 215
BstXI CCANNNNNNTGG 2 cut(s) 55, 71
Bsu15I ATCGAT 1 cut(s) 186
BsuTUI ATCGAT 1 cut(s) 186
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 4 cut(s) 64, 64, 112, 324
BtsIMutI CAGTG 1 cut(s) 145
ClaI ATCGAT 1 cut(s) 186
Csp6I GTAC 1 cut(s) 241
CviAII CATG 1 cut(s) 157
CviJI RGCY 2 cut(s) 235, 317
CviKI_1 RGCY 2 cut(s) 235, 317
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 237
DpnI GATC 2 cut(s) 30, 185
DpnII GATC 2 cut(s) 28, 183
EciI GGCGGA 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 67
FaeI CATG 1 cut(s) 160
FaiI YATR 5 cut(s) 39, 158, 174, 217, 305
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 233
FokI GGATG 4 cut(s) 51, 71, 119, 311
Fsp4HI GCNGC 1 cut(s) 233
GluI GCNGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 266
Hin1II CATG 1 cut(s) 160
HphI GGTGA 1 cut(s) 86
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 1 cut(s) 248
HpyAV CCTTC 2 cut(s) 97, 157
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 237
HpySE526I ACGT 1 cut(s) 129
Hsp92II CATG 1 cut(s) 160
Kzo9I GATC 2 cut(s) 28, 183
LpnPI CCDG 6 cut(s) 17, 54, 81, 84, 163, 296
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 3 cut(s) 97, 178, 333
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 2 cut(s) 43, 134
MalI GATC 2 cut(s) 30, 185
MboI GATC 2 cut(s) 28, 183
MboII GAAGA 2 cut(s) 137, 220
MluCI AATT 1 cut(s) 16
MnlI CCTC 3 cut(s) 202, 234, 246
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 1 cut(s) 349
MslI CAYNNNNRTG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 69
MvaI CCWGG 1 cut(s) 69
NdeII GATC 2 cut(s) 28, 183
NlaIII CATG 1 cut(s) 160
NmuCI GTSAC 2 cut(s) 43, 134
NspI RCATGY 1 cut(s) 160
NspV TTCGAA 1 cut(s) 123
PciI ACATGT 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 167
PkrI GCNGC 1 cut(s) 234
PscI ACATGT 1 cut(s) 156
PshAI GACNNNNGTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 67
PspGI CCWGG 1 cut(s) 67
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 349
SatI GCNGC 1 cut(s) 233
Sau3AI GATC 2 cut(s) 28, 183
ScrFI CCNGG 1 cut(s) 69
SetI ASST 2 cut(s) 36, 132
SfaNI GCATC 3 cut(s) 97, 178, 333
SfuI TTCGAA 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 69
Sse9I AATT 1 cut(s) 16
SsiI CCGC 1 cut(s) 328
StyD4I CCNGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 140
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 123, 186
TasI AATT 1 cut(s) 16
TatI WGTACW 1 cut(s) 240
Tru1I TTAA 1 cut(s) 349
Tru9I TTAA 1 cut(s) 349
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 2 cut(s) 43, 134
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 2 cut(s) 43, 134
TspDTI ATGAA 1 cut(s) 256
TspRI CASTG 1 cut(s) 145
XceI RCATGY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 310
XmnI GAANNNNTTC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.