Rh4AG010300

Cytochrome b5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0007036
Physical Location & Seq
Reverse (-)
1678566 .. 1682816
4251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG010300.1

Sequence Viewer

Length: 351 bp
ATGTTCTTATTGCCAAATTATTTCTTGGATCAGGTTTATGATGTGACCAAATTCTTGGAAGACCATCCTGGTGGTGATGATGTTTTGTTGTCAGCAACAGGGAAGGATGCAACTGATGATTTCGAAGATGTGGGTCACAGTGATAATGCCAGAGACATGTTGAAGGACTTCTATGTGGGAGAGATCGACGCCTCTACTATCCCCAAGAAGACCACATACACTCCTCCCAAGCAGCCTCAGTACAATCAAGACAAGACTTCAGAGTTCATCATCAAACTTCTCCAGTTCCTTGTTCCCATTGCCATATTGGGTTTGGCTTTTGGCATCCGCCTTTACACCAAGTCATCTTAA

Protein Analysis

116

Amino Acids

13.08

Weight (kDa)

4.57

Isoelectric Point (pI)

26.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 10 - 62 4.2e-21 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 328
AclWI GGATC 1 cut(s) 36
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 1 cut(s) 243
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 1 cut(s) 242
AflIII ACRYGT 1 cut(s) 156
AgsI TTSAA 1 cut(s) 163
AjnI CCWGG 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 1 cut(s) 36
ApeKI GCWGC 1 cut(s) 232
ApoI RAATTY 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 86
AsuII TTCGAA 1 cut(s) 123
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
BbsI GAAGAC 2 cut(s) 66, 215
BbvI GCAGC 1 cut(s) 244
BccI CCATC 1 cut(s) 72
BciT130I CCWGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 147
BisI GCNGC 1 cut(s) 233
BlsI GCNGC 1 cut(s) 234
Bme1390I CCNGG 1 cut(s) 69
BmrFI CCNGG 1 cut(s) 69
BmsI GCATC 2 cut(s) 97, 333
BpiI GAAGAC 2 cut(s) 66, 215
BpmI CTGGAG 1 cut(s) 266
Bpu14I TTCGAA 1 cut(s) 123
BsaHI GRCGYC 1 cut(s) 189
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bse1I ACTGG 1 cut(s) 283
Bse3DI GCAATG 1 cut(s) 297
BseBI CCWGG 1 cut(s) 69
BseGI GGATG 3 cut(s) 64, 112, 324
BseMI GCAATG 1 cut(s) 297
BseMII CTCAG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 1 cut(s) 213
BseXI GCAGC 1 cut(s) 244
BsmAI GTCTC 1 cut(s) 147
Bsp119I TTCGAA 1 cut(s) 123
Bsp143I GATC 2 cut(s) 28, 183
BspACI CCGC 1 cut(s) 328
BspCNI CTCAG 1 cut(s) 250
BspPI GGATC 1 cut(s) 36
BspT104I TTCGAA 1 cut(s) 123
BsrDI GCAATG 1 cut(s) 297
BsrI ACTGG 1 cut(s) 283
BssMI GATC 2 cut(s) 28, 183
BssNI GRCGYC 1 cut(s) 189
Bst2UI CCWGG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 140
BstACI GRCGYC 1 cut(s) 189
BstBI TTCGAA 1 cut(s) 123
BstDEI CTNAG 1 cut(s) 237
BstF5I GGATG 3 cut(s) 64, 112, 324
BstKTI GATC 2 cut(s) 31, 186
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 2 cut(s) 28, 183
BstNI CCWGG 1 cut(s) 69
BstNSI RCATGY 1 cut(s) 160
BstSCI CCNGG 1 cut(s) 67
BstV1I GCAGC 1 cut(s) 244
BstV2I GAAGAC 2 cut(s) 66, 215
BstXI CCANNNNNNTGG 2 cut(s) 55, 71
BtsCI GGATG 3 cut(s) 64, 112, 324
BtsIMutI CAGTG 1 cut(s) 145
CseI GACGC 1 cut(s) 197
Csp6I GTAC 1 cut(s) 241
CviAII CATG 1 cut(s) 157
CviJI RGCY 2 cut(s) 235, 317
CviKI_1 RGCY 2 cut(s) 235, 317
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 1 cut(s) 237
DpnI GATC 2 cut(s) 30, 185
DpnII GATC 2 cut(s) 28, 183
EciI GGCGGA 1 cut(s) 317
Eco57I CTGAAG 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 67
FaeI CATG 1 cut(s) 160
FaiI YATR 5 cut(s) 39, 158, 174, 217, 305
FatI CATG 1 cut(s) 156
Fnu4HI GCNGC 1 cut(s) 233
FokI GGATG 3 cut(s) 51, 119, 311
Fsp4HI GCNGC 1 cut(s) 233
GluI GCNGC 1 cut(s) 233
GsuI CTGGAG 1 cut(s) 266
HgaI GACGC 1 cut(s) 197
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 1 cut(s) 160
HphI GGTGA 1 cut(s) 86
Hpy188I TCNGA 1 cut(s) 262
Hpy188III TCNNGA 1 cut(s) 248
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 97, 157
HpyCH4III ACNGT 1 cut(s) 140
HpyCH4V TGCA 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 237
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 1 cut(s) 160
Kzo9I GATC 2 cut(s) 28, 183
LpnPI CCDG 6 cut(s) 17, 54, 81, 84, 163, 296
Lsp1109I GCAGC 1 cut(s) 244
LweI GCATC 2 cut(s) 97, 333
MaeIII GTNAC 2 cut(s) 43, 134
MalI GATC 2 cut(s) 30, 185
MboI GATC 2 cut(s) 28, 183
MboII GAAGA 3 cut(s) 71, 137, 220
MluCI AATT 2 cut(s) 16, 50
MnlI CCTC 3 cut(s) 202, 234, 246
MroXI GAANNNNTTC 1 cut(s) 167
MseI TTAA 1 cut(s) 349
MslI CAYNNNNRTG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 69
MvaI CCWGG 1 cut(s) 69
NdeII GATC 2 cut(s) 28, 183
NlaIII CATG 1 cut(s) 160
NmuCI GTSAC 2 cut(s) 43, 134
NspI RCATGY 1 cut(s) 160
NspV TTCGAA 1 cut(s) 123
PciI ACATGT 1 cut(s) 156
PdmI GAANNNNTTC 1 cut(s) 167
PkrI GCNGC 1 cut(s) 234
PscI ACATGT 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 67
PspGI CCWGG 1 cut(s) 67
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
RseI CAYNNNNRTG 1 cut(s) 69
SaqAI TTAA 1 cut(s) 349
SatI GCNGC 1 cut(s) 233
Sau3AI GATC 2 cut(s) 28, 183
ScrFI CCNGG 1 cut(s) 69
SetI ASST 1 cut(s) 36
SfaNI GCATC 2 cut(s) 97, 333
SfuI TTCGAA 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 69
Sse9I AATT 2 cut(s) 16, 50
SsiI CCGC 1 cut(s) 328
StyD4I CCNGG 1 cut(s) 67
TaaI ACNGT 1 cut(s) 140
TaqI TCGA 2 cut(s) 123, 186
TasI AATT 2 cut(s) 16, 50
TatI WGTACW 1 cut(s) 240
Tru1I TTAA 1 cut(s) 349
Tru9I TTAA 1 cut(s) 349
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 2 cut(s) 43, 134
TseI GCWGC 1 cut(s) 232
Tsp45I GTSAC 2 cut(s) 43, 134
TspDTI ATGAA 1 cut(s) 256
TspRI CASTG 1 cut(s) 145
XapI RAATTY 1 cut(s) 50
XceI RCATGY 1 cut(s) 160
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 310
XmnI GAANNNNTTC 1 cut(s) 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.