AT2G39220

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008401
Physical Location & Seq
Reverse (-)
16374881 .. 16377168
2288 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G39220.1

Sequence Viewer

Length: 1500 bp
ATGTTAACTACGATGCAAAGAGTACACAACAAACCGATTGATTCTATCGGAGGTTTTAAGCATTTGGTAAAACAGAGTAACGGCGGTGACGGTGGAGTGACGGCGACGGATATGCAAGAACCGAGCATCGAGACTGATAAGCTTAGCTACGAGATTTTCTCTATTCTCGAAAGCAAGTTTCTTTTCGGGTATGATGATGACCTGAAGCTTATGGAATCAAGAAGCAGAGACCCGAGTCCAGAGCAAGAAACGGCATCACCGGCGATGGTGGAAGCTTTAAACGGCGTTGTTCCGGGTACAGTTAAGAACCAGAGAGGTAAAGTTTGTGTTTTGAGTATCGATAGTGGTGGAATGAGAGGGATTATACCTGGAAAAGCTTTGGCTTATCTGGAACATGCTCTGAAATCGAAATCGGGTGACCCGAATGCTCGAATCGCTGATTACTTCGACGTAGCTTCCGGTTCCGGTATCGGTGGGATTTTCACGGCGATGCTTTTTGCGTCGAGCGATGGTAACCGTCCGATCTTCAAAGCGGAAGACACGTGGCGGTTTTTAGCTATGAAAGGTAAAAGCTTTTACAACAAATCACCACCGGGGATACTAAACAGGGTTATGAAAACCGGGTCGGGTGGTTCGGGTGGGTCAGGTTCGAAGCTTGAGAAAGCGATGAAGGAGTCTTTCGAAGAGCTTACGCTTAAGGATACGCTTAAACCGGTTTTGATTCCTTGTTACGACCTCACGAGCTCTGCACCGTTCTTGTTCTCACGTGCTGATGCGTTGGAAACGGACGGTTATGATTTTAAGCTGTGGGAAGTTTGTAGAGCTACTTGGGCTGAGCCAGGAGTGTTTGAGCCGGTGGAGATGAGATCAGTTGATGGTAAGACACGGTGTGTAGCTGTTGATGGTGGTTTAGCAATGAGTAATCCAACGGCTGCAGCGATTACTCATGTGTTGCATAATAAGCAAGAGTTTCCTTTTGTTAGAGGTGTTGAGGATTTGCTTGTGTTGTCACTTGGTACGGGACAGTTGGTTGATGTTAAGTATGATTGTGATAAGGTTATGAAGTGGAAGGCTAAGCATTGGGCTCGACCTGCGGTTCGGATTTCTGCTGATGGAGCAGCTGATACTGTGGATCAGGCTGTTTCCATGGCGTTTGGTCAGTGTAGGAGGAGTAACTACGTCCGTATTCAGGCGAATGGATCAAGCTTTGGTCCGTGCAAACCAAACATAGACACAGATGCGAGTCCAAGCAATGTGAATATGCTTGTGGGAGTAGCAGAGGAGATGCTGAAGCAGAAGAATGCAGAGTCTGTTCTGTTTGGTGGTAAGAAAATCAATGAAGAAAGCAATTATGAGAAGCTGGATTGGTTAGCCGGTGAACTAGTGCTAGAACACCAGAGGAGGAGTTGTAGAATCGCTCCCACTGTAGCGTTCAAGCAATCCGGCGACAGAAGAGTCGATCAACAGACCATTTTCAAGGATATTGATTGTATGTTTTGA

Protein Analysis

499

Amino Acids

54.49

Weight (kDa)

6.24

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 111 - 314 3.8e-22 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1454
Acc36I ACCTGC 1 cut(s) 1099
AciI CCGC 4 cut(s) 84, 533, 547, 1094
AclWI GGATC 2 cut(s) 1140, 1207
AcuI CTGAAG 2 cut(s) 224, 1310
AcvI CACGTG 2 cut(s) 543, 767
AdeI CACNNNGTG 1 cut(s) 890
AfaI GTAC 3 cut(s) 24, 298, 1018
AfiI CCNNNNNNNGG 1 cut(s) 1189
AflII CTTAAG 1 cut(s) 695
AflIII ACRYGT 1 cut(s) 540
AgeI ACCGGT 1 cut(s) 712
AgsI TTSAA 3 cut(s) 529, 1435, 1477
AhlI ACTAGT 1 cut(s) 1381
AjnI CCWGG 2 cut(s) 367, 838
Alw21I GWGCWC 1 cut(s) 746
Alw26I GTCTC 2 cut(s) 125, 222
AlwI GGATC 2 cut(s) 1140, 1207
AlwNI CAGNNNCTG 1 cut(s) 1310
Ama87I CYCGRG 1 cut(s) 232
ApeKI GCWGC 3 cut(s) 932, 935, 1118
ArsI GACNNNNNNTTYG 2 cut(s) 1080, 1112
AsiGI ACCGGT 1 cut(s) 712
AspS9I GGNCC 1 cut(s) 1211
AsuC2I CCSGG 3 cut(s) 294, 594, 622
AsuHPI GGTGA 5 cut(s) 98, 249, 428, 579, 1388
AsuII TTCGAA 2 cut(s) 650, 681
AvaI CYCGRG 1 cut(s) 232
AvaII GGWCC 1 cut(s) 1211
BanII GRGCYC 2 cut(s) 746, 1087
BauI CACGAG 1 cut(s) 739
BbrPI CACGTG 2 cut(s) 543, 767
BbsI GAAGAC 1 cut(s) 543
Bbv12I GWGCWC 1 cut(s) 746
BbvI GCAGC 3 cut(s) 919, 947, 1130
BccI CCATC 5 cut(s) 259, 503, 869, 896, 1106
BceAI ACGGC 6 cut(s) 97, 117, 267, 298, 501, 945
BcgI CGANNNNNNTGC 4 cut(s) 94, 128, 1067, 1101
BciT130I CCWGG 2 cut(s) 369, 840
BciVI GTATCC 2 cut(s) 591, 694
BcnI CCSGG 3 cut(s) 294, 594, 622
BcoDI GTCTC 2 cut(s) 125, 222
BcuI ACTAGT 1 cut(s) 1381
BfaI CTAG 2 cut(s) 1382, 1388
BfmI CTRYAG 2 cut(s) 933, 1425
BfrI CTTAAG 1 cut(s) 695
BfuAI ACCTGC 1 cut(s) 1099
BfuI GTATCC 2 cut(s) 591, 694
BisI GCNGC 3 cut(s) 933, 936, 1119
BlpI GCTNAGC 3 cut(s) 143, 834, 1074
BlsI GCNGC 3 cut(s) 934, 937, 1120
Bme1390I CCNGG 5 cut(s) 294, 369, 594, 622, 840
Bme18I GGWCC 1 cut(s) 1211
BmeT110I CYCGRG 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 1211
BmiI GGNNCC 1 cut(s) 463
BmrFI CCNGG 5 cut(s) 294, 369, 594, 622, 840
BmsI GCATC 7 cut(s) 3, 135, 263, 480, 763, 1228, 1275
BoxI GACNNNNGTC 1 cut(s) 234
BpiI GAAGAC 1 cut(s) 543
BplI GAGNNNNNCTC 2 cut(s) 143, 175
Bpu1102I GCTNAGC 3 cut(s) 143, 834, 1074
Bpu14I TTCGAA 2 cut(s) 650, 681
BpuEI CTTGAG 1 cut(s) 677
BpuMI CCSGG 3 cut(s) 294, 594, 622
Bsa29I ATCGAT 1 cut(s) 339
BsaAI YACGTR 2 cut(s) 543, 767
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 593, 1146
BsaWI WCCGGW 3 cut(s) 458, 464, 712
BsaXI ACNNNNNCTCC 2 cut(s) 1393, 1423
Bsc4I CCNNNNNNNGG 1 cut(s) 1189
Bse118I RCCGGY 4 cut(s) 259, 712, 853, 1373
Bse3DI GCAATG 2 cut(s) 921, 1258
BseBI CCWGG 2 cut(s) 369, 840
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 593, 1146
BseLI CCNNNNNNNGG 1 cut(s) 1189
BseMI GCAATG 2 cut(s) 921, 1258
BseMII CTCAG 1 cut(s) 825
BseRI GAGGAG 4 cut(s) 1183, 1295, 1414, 1417
BseXI GCAGC 3 cut(s) 919, 947, 1130
BsgI GTGCAG 1 cut(s) 732
BshTI ACCGGT 1 cut(s) 712
BshVI ATCGAT 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 746
BsiHKCI CYCGRG 1 cut(s) 232
BslFI GGGAC 1 cut(s) 1035
BslI CCNNNNNNNGG 1 cut(s) 1189
BsmAI GTCTC 2 cut(s) 125, 222
BsmFI GGGAC 1 cut(s) 1035
BsmI GAATGC 2 cut(s) 430, 1306
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 1 cut(s) 232
Bsp119I TTCGAA 2 cut(s) 650, 681
Bsp1286I GDGCHC 2 cut(s) 746, 1087
Bsp143I GATC 5 cut(s) 522, 866, 1132, 1199, 1459
Bsp1720I GCTNAGC 3 cut(s) 143, 834, 1074
Bsp19I CCATGG 1 cut(s) 1146
BspACI CCGC 4 cut(s) 84, 533, 547, 1094
BspCNI CTCAG 1 cut(s) 826
BspDI ATCGAT 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 463
BspMAI CTGCAG 1 cut(s) 937
BspMI ACCTGC 1 cut(s) 1099
BspPI GGATC 2 cut(s) 1140, 1207
BspQI GCTCTTC 1 cut(s) 678
BspT104I TTCGAA 2 cut(s) 650, 681
BspTI CTTAAG 1 cut(s) 695
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 2 cut(s) 921, 1258
BsrFI RCCGGY 4 cut(s) 259, 712, 853, 1373
BssAI RCCGGY 4 cut(s) 259, 712, 853, 1373
BssECI CCNNGG 2 cut(s) 593, 1146
BssMI GATC 5 cut(s) 522, 866, 1132, 1199, 1459
BssSI CACGAG 1 cut(s) 739
BssT1I CCWWGG 1 cut(s) 1146
Bst2BI CACGAG 1 cut(s) 739
Bst2UI CCWGG 2 cut(s) 369, 840
Bst4CI ACNGT 9 cut(s) 92, 301, 518, 753, 791, 888, 1026, 1129, 1426
Bst6I CTCTTC 2 cut(s) 678, 1447
BstAFI CTTAAG 1 cut(s) 695
BstBAI YACGTR 2 cut(s) 543, 767
BstBI TTCGAA 2 cut(s) 650, 681
BstDEI CTNAG 3 cut(s) 143, 834, 1074
BstDSI CCRYGG 1 cut(s) 1146
BstEII GGTNACC 2 cut(s) 416, 512
BstKTI GATC 5 cut(s) 525, 869, 1135, 1202, 1462
BstMAI GTCTC 2 cut(s) 125, 222
BstMBI GATC 5 cut(s) 522, 866, 1132, 1199, 1459
BstMWI GCNNNNNNNGC 6 cut(s) 260, 434, 830, 961, 1091, 1115
BstNI CCWGG 2 cut(s) 369, 840
BstNSI RCATGY 1 cut(s) 398
BstPAI GACNNNNGTC 1 cut(s) 234
BstPI GGTNACC 2 cut(s) 416, 512
BstSCI CCNGG 5 cut(s) 292, 367, 592, 620, 838
BstSFI CTRYAG 2 cut(s) 933, 1425
BstV1I GCAGC 3 cut(s) 919, 947, 1130
BstV2I GAAGAC 1 cut(s) 543
Bsu15I ATCGAT 1 cut(s) 339
BsuI GTATCC 2 cut(s) 591, 694
BsuTUI ATCGAT 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 1146
BtgZI GCGATG 4 cut(s) 278, 503, 522, 680
BtsIMutI CAGTG 2 cut(s) 1166, 1422
BveI ACCTGC 1 cut(s) 1099
CaiI CAGNNNCTG 1 cut(s) 1310
Cfr10I RCCGGY 4 cut(s) 259, 712, 853, 1373
Cfr13I GGNCC 1 cut(s) 1211
ClaI ATCGAT 1 cut(s) 339
CseI GACGC 1 cut(s) 489
Csp6I GTAC 3 cut(s) 23, 297, 1017
CspAI ACCGGT 1 cut(s) 712
CviAII CATG 3 cut(s) 395, 947, 1147
CviQI GTAC 3 cut(s) 23, 297, 1017
DdeI CTNAG 3 cut(s) 143, 834, 1074
DpnI GATC 5 cut(s) 524, 868, 1134, 1201, 1461
DpnII GATC 5 cut(s) 522, 866, 1132, 1199, 1459
DraI TTTAAA 1 cut(s) 279
DraIII CACNNNGTG 1 cut(s) 890
DrdI GACNNNNNNGTC 1 cut(s) 1454
DseDI GACNNNNNNGTC 1 cut(s) 1454
Eam1104I CTCTTC 2 cut(s) 678, 1447
EarI CTCTTC 2 cut(s) 678, 1447
Ecl136II GAGCTC 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 1146
Eco24I GRGCYC 2 cut(s) 746, 1087
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 1211
Eco53kI GAGCTC 1 cut(s) 744
Eco57I CTGAAG 2 cut(s) 224, 1310
Eco72I CACGTG 2 cut(s) 543, 767
Eco88I CYCGRG 1 cut(s) 232
Eco91I GGTNACC 2 cut(s) 416, 512
EcoICRI GAGCTC 1 cut(s) 744
EcoO65I GGTNACC 2 cut(s) 416, 512
EcoRII CCWGG 2 cut(s) 367, 838
EcoT14I CCWWGG 1 cut(s) 1146
EcoT38I GRGCYC 2 cut(s) 746, 1087
ErhI CCWWGG 1 cut(s) 1146
FaeI CATG 3 cut(s) 398, 950, 1150
FaqI GGGAC 1 cut(s) 1035
FatI CATG 3 cut(s) 394, 946, 1146
Fnu4HI GCNGC 3 cut(s) 933, 936, 1119
FriOI GRGCYC 2 cut(s) 746, 1087
Fsp4HI GCNGC 3 cut(s) 933, 936, 1119
FspBI CTAG 2 cut(s) 1382, 1388
GluI GCNGC 3 cut(s) 933, 936, 1119
HgaI GACGC 1 cut(s) 489
Hin1II CATG 3 cut(s) 398, 950, 1150
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 7 cut(s) 140, 206, 273, 375, 571, 653, 1204
HpaI GTTAAC 1 cut(s) 6
HphI GGTGA 5 cut(s) 98, 249, 428, 579, 1388
Hpy166II GTNNAC 3 cut(s) 6, 25, 1379
Hpy188I TCNGA 4 cut(s) 50, 402, 522, 1101
Hpy188III TCNNGA 6 cut(s) 130, 167, 219, 239, 389, 739
Hpy8I GTNNAC 3 cut(s) 6, 25, 1379
Hpy99I CGWCG 3 cut(s) 109, 452, 505
HpyAV CCTTC 2 cut(s) 664, 1063
HpyCH4III ACNGT 9 cut(s) 92, 301, 518, 753, 791, 888, 1026, 1129, 1426
HpyCH4IV ACGT 4 cut(s) 450, 542, 766, 1179
HpyCH4V TGCA 7 cut(s) 16, 115, 749, 935, 955, 1218, 1304
HpyF10VI GCNNNNNNNGC 6 cut(s) 260, 434, 830, 961, 1091, 1115
HpyF3I CTNAG 3 cut(s) 143, 834, 1074
HpySE526I ACGT 4 cut(s) 450, 542, 766, 1179
Hsp92II CATG 3 cut(s) 398, 950, 1150
KspAI GTTAAC 1 cut(s) 6
Kzo9I GATC 5 cut(s) 522, 866, 1132, 1199, 1459
LguI GCTCTTC 1 cut(s) 678
LmnI GCTCC 2 cut(s) 1115, 1423
Lsp1109I GCAGC 3 cut(s) 919, 947, 1130
LweI GCATC 7 cut(s) 3, 135, 263, 480, 763, 1228, 1275
MaeI CTAG 2 cut(s) 1382, 1388
MaeII ACGT 4 cut(s) 450, 542, 766, 1179
MaeIII GTNAC 8 cut(s) 77, 86, 97, 416, 512, 728, 1008, 1172
MalI GATC 5 cut(s) 524, 868, 1134, 1201, 1461
MboI GATC 5 cut(s) 522, 866, 1132, 1199, 1459
MboII GAAGA 6 cut(s) 517, 548, 695, 1309, 1352, 1464
MhlI GDGCHC 2 cut(s) 746, 1087
MluCI AATT 1 cut(s) 1348
MlyI GAGTC 5 cut(s) 244, 683, 1252, 1316, 1464
MmeI TCCRAC 2 cut(s) 759, 950
MseI TTAA 8 cut(s) 5, 57, 278, 303, 696, 708, 801, 1038
MslI CAYNNNNRTG 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 1121
MspCI CTTAAG 1 cut(s) 695
MspR9I CCNGG 5 cut(s) 294, 369, 594, 622, 840
Mva1269I GAATGC 2 cut(s) 430, 1306
MvaI CCWGG 2 cut(s) 369, 840
MwoI GCNNNNNNNGC 6 cut(s) 260, 434, 830, 961, 1091, 1115
NciI CCSGG 3 cut(s) 294, 594, 622
NcoI CCATGG 1 cut(s) 1146
NdeII GATC 5 cut(s) 522, 866, 1132, 1199, 1459
NlaIII CATG 3 cut(s) 398, 950, 1150
NlaIV GGNNCC 1 cut(s) 463
NmuCI GTSAC 4 cut(s) 86, 97, 416, 1008
NspI RCATGY 1 cut(s) 398
NspV TTCGAA 2 cut(s) 650, 681
PciSI GCTCTTC 1 cut(s) 678
PcsI WCGNNNNNNNCGW 2 cut(s) 419, 935
PctI GAATGC 2 cut(s) 430, 1306
PfeI GAWTC 5 cut(s) 41, 215, 432, 721, 1413
PinAI ACCGGT 1 cut(s) 712
PkrI GCNGC 3 cut(s) 934, 937, 1120
PleI GAGTC 5 cut(s) 243, 682, 1251, 1315, 1463
PmaCI CACGTG 2 cut(s) 543, 767
PmlI CACGTG 2 cut(s) 543, 767
PpsI GAGTC 5 cut(s) 243, 682, 1251, 1315, 1463
Ppu21I YACGTR 2 cut(s) 543, 767
PshAI GACNNNNGTC 1 cut(s) 234
Psp124BI GAGCTC 1 cut(s) 746
Psp6I CCWGG 2 cut(s) 367, 838
PspCI CACGTG 2 cut(s) 543, 767
PspEI GGTNACC 2 cut(s) 416, 512
PspGI CCWGG 2 cut(s) 367, 838
PspN4I GGNNCC 1 cut(s) 463
PspPI GGNCC 1 cut(s) 1211
PstI CTGCAG 1 cut(s) 937
PstNI CAGNNNCTG 1 cut(s) 1310
PvuII CAGCTG 1 cut(s) 1121
RsaI GTAC 3 cut(s) 24, 298, 1018
RsaNI GTAC 3 cut(s) 23, 297, 1017
RseI CAYNNNNRTG 1 cut(s) 488
SacI GAGCTC 1 cut(s) 746
SapI GCTCTTC 1 cut(s) 678
SaqAI TTAA 8 cut(s) 5, 57, 278, 303, 696, 708, 801, 1038
SatI GCNGC 3 cut(s) 933, 936, 1119
Sau3AI GATC 5 cut(s) 522, 866, 1132, 1199, 1459
Sau96I GGNCC 1 cut(s) 1211
SchI GAGTC 5 cut(s) 244, 683, 1252, 1316, 1464
ScrFI CCNGG 5 cut(s) 294, 369, 594, 622, 840
SduI GDGCHC 2 cut(s) 746, 1087
SfaNI GCATC 7 cut(s) 3, 135, 263, 480, 763, 1228, 1275
SfcI CTRYAG 2 cut(s) 933, 1425
SfuI TTCGAA 2 cut(s) 650, 681
SgrAI CRCCGGYG 1 cut(s) 259
SinI GGWCC 1 cut(s) 1211
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 2 cut(s) 656, 695
SmoI CTYRAG 2 cut(s) 656, 695
SpeI ACTAGT 1 cut(s) 1381
Sse9I AATT 1 cut(s) 1348
SsiI CCGC 4 cut(s) 84, 533, 547, 1094
SspMI CTAG 2 cut(s) 1382, 1388
SstI GAGCTC 1 cut(s) 746
StyD4I CCNGG 5 cut(s) 292, 367, 592, 620, 838
StyI CCWWGG 1 cut(s) 1146
TaaI ACNGT 9 cut(s) 92, 301, 518, 753, 791, 888, 1026, 1129, 1426
TaiI ACGT 4 cut(s) 453, 545, 769, 1182
TasI AATT 1 cut(s) 1348
TatI WGTACW 1 cut(s) 22
TfiI GAWTC 5 cut(s) 41, 215, 432, 721, 1413
Tru1I TTAA 8 cut(s) 5, 57, 278, 303, 696, 708, 801, 1038
Tru9I TTAA 8 cut(s) 5, 57, 278, 303, 696, 708, 801, 1038
TscAI CASTG 2 cut(s) 1166, 1429
TseFI GTSAC 4 cut(s) 86, 97, 416, 1008
TseI GCWGC 3 cut(s) 932, 935, 1118
Tsp45I GTSAC 4 cut(s) 86, 97, 416, 1008
TspDTI ATGAA 5 cut(s) 575, 629, 683, 1076, 1353
TspGWI ACGGA 4 cut(s) 122, 800, 1172, 1203
TspRI CASTG 2 cut(s) 1166, 1429
Vha464I CTTAAG 1 cut(s) 695
VpaK11BI GGWCC 1 cut(s) 1211
XceI RCATGY 1 cut(s) 398
XspI CTAG 2 cut(s) 1382, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.