pycom01g13590

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008401
Physical Location & Seq
Forward (+)
14009638 .. 14012380
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g13590.1

Sequence Viewer

Length: 1155 bp
ATGAGAGGGATTCTCTCCGGAAAGGCCTTGGCGTATCTAGAGCAAGCGTTGAAGCAGAAATCGGGGAATCCGGACGCCAGAATCGCCGATTACTTCGACGTCGCCACTGGAGCTGGCGTCGGAGGTATATTCACCGCCATGCTCTTCGCCACCAGAGACCACGCCCGCCCGATTTTCAAGGCCGACGACACGTGGCGCTTCCTCTCCGATCACGGCAAGAGCTTGTTCAACAATAACCGCTCCTCCACCTCCGCCTCCTCCCCCGGTTTGCTGCGTCGCCTCATTCGAGGTTCTGGTTCGGGCTCAGGTTCAACGGGGTCCGCCACGGCCGGTTTGGAAAAAGTGATGAAAGAGGCGTTCGTCGACGACGACAGGACCTTGACGTTGAAAGACACGCTCAAGCCGGTTCTAATTCCGTGCTATGACCTCTCCAGTACGGCGCCGTTTCTCTTCTCCAGGGCGGACGCCCTCGAGACCGACAGCTACGACTTCCGCCTCTGGGAGGTCTGCCGCGCCACGTCAGCCGAACCGGGAGTCTTCGAACCGGTCCAAATGCAGTCCGTCAACAACCAGACCAAGTGCTTGGCCGTGGACGGCGGCTTGGCCATGAGCAACCCGACCGCGGCGGCGATCACGCACGTCCTGCACAACAAGCAGGAGTTCCCGTTCGTGCGAGGGGTCGAGGACATAATGGTCCTTTCAATAGGAAGCGGCCAGTTTCTGGAAGAAGCGAGGTACGCGTACGAGCAAGTGAAGAAGTGGCGAGCCAAGGAATGGGCCCGGCCCATGGCCCGAATCGCCGGCGACGGGTCATCCGACCTGGTAGACCAGTCCGTCGCCATGGCGTTTGGTCAGTCCGGGAGCAGCAACTACGTGCGCATTCAGGCAAATGGGACAAGCTTAGGACGTTGCGGGCCAAATGTAGATACAGACCCAAGTCCTAACAACGTTAAGAAGCTGGTAGGAGTGGCAGAGGAAATGCTGAAACAGAAGAATGTTGAATCGGTGCTCTTTGGCGGTAAGAGGATCGGCGAGCAGAGCAATTTTGAGAAGCTCGATTGGTTTGCCGGAGAACTGGTGCTCGAGCATCAGAGGAGGAGTTGCAGAATAGCTCCCACTGTTGCCTTCAAGCAAGCTGCTCCCAAAACAAGTTAG

Protein Analysis

385

Amino Acids

41.72

Weight (kDa)

8.49

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 1 - 211 1.7e-17 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 692, 833
AatII GACGTC 1 cut(s) 102
Acc16I TGCGCA 1 cut(s) 878
AccB1I GGYRCC 1 cut(s) 439
AccB7I CCANNNNNTGG 2 cut(s) 721, 774
AccBSI CCGCTC 1 cut(s) 240
AccI GTMKAC 2 cut(s) 363, 825
AccII CGCG 3 cut(s) 513, 623, 740
AccIII TCCGGA 2 cut(s) 17, 70
AclI AACGTT 1 cut(s) 948
AclWI GGATC 1 cut(s) 1034
AcoI YGGCCR 4 cut(s) 327, 585, 603, 712
AcvI CACGTG 1 cut(s) 192
AcyI GRCGYC 5 cut(s) 75, 99, 117, 440, 465
AfaI GTAC 3 cut(s) 436, 737, 743
AfiI CCNNNNNNNGG 7 cut(s) 499, 502, 622, 721, 774, 787, 807
AflIII ACRYGT 2 cut(s) 189, 738
AgeI ACCGGT 1 cut(s) 544
AgsI TTSAA 8 cut(s) 52, 178, 229, 312, 388, 702, 1001, 1129
AjiI CACGTC 2 cut(s) 519, 640
AjnI CCWGG 2 cut(s) 455, 819
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 8 cut(s) 113, 222, 483, 900, 958, 1054, 1112, 1136
AluI AGCT 8 cut(s) 113, 222, 483, 900, 958, 1054, 1112, 1136
Alw21I GWGCWC 2 cut(s) 1011, 1083
Alw26I GTCTC 2 cut(s) 150, 467
AlwI GGATC 1 cut(s) 1034
AlwNI CAGNNNCTG 1 cut(s) 721
Ama87I CYCGRG 2 cut(s) 470, 1082
Aor13HI TCCGGA 2 cut(s) 17, 70
ApaI GGGCCC 1 cut(s) 781
ApeKI GCWGC 3 cut(s) 271, 864, 1136
ArsI GACNNNNNNTTYG 2 cut(s) 519, 551
AsiGI ACCGGT 1 cut(s) 544
AspLEI GCGC 4 cut(s) 198, 442, 515, 879
AspS9I GGNCC 9 cut(s) 318, 375, 547, 694, 777, 778, 783, 790, 914
AsuC2I CCSGG 4 cut(s) 264, 531, 781, 859
AsuHPI GGTGA 1 cut(s) 124
AsuII TTCGAA 1 cut(s) 540
AvaI CYCGRG 2 cut(s) 470, 1082
AvaII GGWCC 4 cut(s) 318, 375, 547, 694
BaeGI GKGCMC 1 cut(s) 781
BalI TGGCCA 1 cut(s) 605
BanI GGYRCC 1 cut(s) 439
BanII GRGCYC 2 cut(s) 305, 781
BbrPI CACGTG 1 cut(s) 192
BbsI GAAGAC 1 cut(s) 529
Bbv12I GWGCWC 2 cut(s) 1011, 1083
BbvI GCAGC 3 cut(s) 258, 876, 1123
BceAI ACGGC 6 cut(s) 229, 342, 427, 453, 572, 610
BcgI CGANNNNNNTGC 2 cut(s) 1046, 1080
BciT130I CCWGG 2 cut(s) 457, 821
BcnI CCSGG 4 cut(s) 264, 531, 781, 859
BcoDI GTCTC 2 cut(s) 150, 467
BfaI CTAG 1 cut(s) 38
BfoI RGCGCY 2 cut(s) 199, 443
BisI GCNGC 8 cut(s) 272, 511, 598, 624, 627, 712, 865, 1137
BlsI GCNGC 8 cut(s) 273, 512, 599, 625, 628, 713, 866, 1138
Bme1390I CCNGG 6 cut(s) 264, 457, 531, 781, 821, 859
Bme18I GGWCC 4 cut(s) 318, 375, 547, 694
BmeT110I CYCGRG 2 cut(s) 470, 1082
BmgBI CACGTC 2 cut(s) 519, 640
BmgT120I GGNCC 9 cut(s) 318, 375, 547, 694, 777, 778, 783, 790, 914
BmiI GGNNCC 3 cut(s) 319, 441, 779
BmrFI CCNGG 6 cut(s) 264, 457, 531, 781, 821, 859
BmsI GCATC 1 cut(s) 1096
BoxI GACNNNNGTC 1 cut(s) 936
BpiI GAAGAC 1 cut(s) 529
BplI GAGNNNNNCTC 1 cut(s) 29
BpmI CTGGAG 3 cut(s) 129, 415, 439
Bpu10I CCTNAGC 2 cut(s) 304, 901
Bpu14I TTCGAA 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 383
BpuMI CCSGG 4 cut(s) 264, 531, 781, 859
BsaAI YACGTR 2 cut(s) 192, 874
BsaHI GRCGYC 5 cut(s) 75, 99, 117, 440, 465
BsaI GGTCTC 2 cut(s) 150, 467
BsaJI CCNNGG 9 cut(s) 27, 262, 324, 456, 588, 621, 768, 786, 840
BsaWI WCCGGW 3 cut(s) 17, 70, 544
BsaXI ACNNNNNCTCC 8 cut(s) 102, 132, 227, 257, 650, 680, 1062, 1092
Bsc4I CCNNNNNNNGG 7 cut(s) 499, 502, 622, 721, 774, 787, 807
Bse118I RCCGGY 4 cut(s) 329, 403, 544, 800
Bse1I ACTGG 5 cut(s) 112, 432, 715, 829, 1080
BseAI TCCGGA 2 cut(s) 17, 70
BseBI CCWGG 2 cut(s) 457, 821
BseDI CCNNGG 9 cut(s) 27, 262, 324, 456, 588, 621, 768, 786, 840
BseGI GGATG 1 cut(s) 812
BseLI CCNNNNNNNGG 7 cut(s) 499, 502, 622, 721, 774, 787, 807
BseMII CTCAG 1 cut(s) 318
BseNI ACTGG 5 cut(s) 112, 432, 715, 829, 1080
BseRI GAGGAG 4 cut(s) 232, 247, 1108, 1111
BseSI GKGCMC 1 cut(s) 781
BseX3I CGGCCG 1 cut(s) 327
BseXI GCAGC 3 cut(s) 258, 876, 1123
BsgI GTGCAG 1 cut(s) 629
Bsh1236I CGCG 3 cut(s) 513, 623, 740
Bsh1285I CGRYCG 2 cut(s) 330, 621
BshNI GGYRCC 1 cut(s) 439
BshTI ACCGGT 1 cut(s) 544
BsiEI CGRYCG 2 cut(s) 330, 621
BsiHKAI GWGCWC 2 cut(s) 1011, 1083
BsiHKCI CYCGRG 2 cut(s) 470, 1082
BsiWI CGTACG 1 cut(s) 741
BslFI GGGAC 1 cut(s) 907
BslI CCNNNNNNNGG 7 cut(s) 499, 502, 622, 721, 774, 787, 807
BsmAI GTCTC 2 cut(s) 150, 467
BsmFI GGGAC 1 cut(s) 907
BsmI GAATGC 1 cut(s) 879
Bso31I GGTCTC 2 cut(s) 150, 467
BsoBI CYCGRG 2 cut(s) 470, 1082
Bsp119I TTCGAA 1 cut(s) 540
Bsp120I GGGCCC 1 cut(s) 777
Bsp1286I GDGCHC 4 cut(s) 305, 781, 1011, 1083
Bsp13I TCCGGA 2 cut(s) 17, 70
Bsp143I GATC 3 cut(s) 208, 630, 1026
Bsp19I CCATGG 2 cut(s) 786, 840
BspCNI CTCAG 1 cut(s) 317
BspEI TCCGGA 2 cut(s) 17, 70
BspFNI CGCG 3 cut(s) 513, 623, 740
BspLI GGNNCC 3 cut(s) 319, 441, 779
BspPI GGATC 1 cut(s) 1034
BspQI GCTCTTC 1 cut(s) 149
BspT104I TTCGAA 1 cut(s) 540
BspT107I GGYRCC 1 cut(s) 439
BspTNI GGTCTC 2 cut(s) 150, 467
BsrBI CCGCTC 1 cut(s) 240
BsrFI RCCGGY 4 cut(s) 329, 403, 544, 800
BsrI ACTGG 5 cut(s) 112, 432, 715, 829, 1080
BssAI RCCGGY 4 cut(s) 329, 403, 544, 800
BssECI CCNNGG 9 cut(s) 27, 262, 324, 456, 588, 621, 768, 786, 840
BssMI GATC 3 cut(s) 208, 630, 1026
BssNI GRCGYC 5 cut(s) 75, 99, 117, 440, 465
BssT1I CCWWGG 4 cut(s) 27, 768, 786, 840
Bst2UI CCWGG 2 cut(s) 457, 821
Bst4CI ACNGT 1 cut(s) 1120
Bst6I CTCTTC 2 cut(s) 149, 455
BstACI GRCGYC 5 cut(s) 75, 99, 117, 440, 465
BstAPI GCANNNNNTGC 1 cut(s) 643
BstBAI YACGTR 2 cut(s) 192, 874
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 8 cut(s) 45, 115, 166, 765, 802, 914, 1034, 1134
BstDEI CTNAG 2 cut(s) 304, 901
BstDSI CCRYGG 5 cut(s) 324, 588, 621, 786, 840
BstENI CCTNNNNNAGG 1 cut(s) 500
BstF5I GGATG 1 cut(s) 812
BstFNI CGCG 3 cut(s) 513, 623, 740
BstH2I RGCGCY 2 cut(s) 199, 443
BstHHI GCGC 4 cut(s) 198, 442, 515, 879
BstKTI GATC 3 cut(s) 211, 633, 1029
BstMAI GTCTC 2 cut(s) 150, 467
BstMBI GATC 3 cut(s) 208, 630, 1026
BstMCI CGRYCG 2 cut(s) 330, 621
BstMWI GCNNNNNNNGC 8 cut(s) 83, 110, 521, 643, 652, 737, 797, 1038
BstNI CCWGG 2 cut(s) 457, 821
BstPAI GACNNNNGTC 1 cut(s) 936
BstSCI CCNGG 6 cut(s) 262, 455, 529, 779, 819, 857
BstSLI GKGCMC 1 cut(s) 781
BstUI CGCG 3 cut(s) 513, 623, 740
BstV1I GCAGC 3 cut(s) 258, 876, 1123
BstV2I GAAGAC 1 cut(s) 529
BstXI CCANNNNNNTGG 1 cut(s) 583
BstZI CGGCCG 1 cut(s) 327
BtgI CCRYGG 5 cut(s) 324, 588, 621, 786, 840
BtrI CACGTC 2 cut(s) 519, 640
BtsCI GGATG 1 cut(s) 812
BtsIMutI CAGTG 2 cut(s) 105, 1116
Cac8I GCNNGC 8 cut(s) 45, 115, 166, 765, 802, 914, 1034, 1134
CaiI CAGNNNCTG 1 cut(s) 721
CfoI GCGC 4 cut(s) 198, 442, 515, 879
Cfr10I RCCGGY 4 cut(s) 329, 403, 544, 800
Cfr13I GGNCC 9 cut(s) 318, 375, 547, 694, 777, 778, 783, 790, 914
Cfr42I CCGCGG 1 cut(s) 624
CseI GACGC 4 cut(s) 83, 106, 263, 473
CsiI ACCWGGT 1 cut(s) 819
Csp6I GTAC 3 cut(s) 435, 736, 742
CspAI ACCGGT 1 cut(s) 544
CviAII CATG 4 cut(s) 139, 607, 787, 841
CviQI GTAC 3 cut(s) 435, 736, 742
DdeI CTNAG 2 cut(s) 304, 901
DinI GGCGCC 1 cut(s) 441
DpnI GATC 3 cut(s) 210, 632, 1028
DpnII GATC 3 cut(s) 208, 630, 1026
DrdI GACNNNNNNGTC 2 cut(s) 692, 833
DseDI GACNNNNNNGTC 2 cut(s) 692, 833
EaeI YGGCCR 4 cut(s) 327, 585, 603, 712
EagI CGGCCG 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 149, 455
EarI CTCTTC 2 cut(s) 149, 455
EciI GGCGGA 4 cut(s) 241, 310, 476, 482
EclXI CGGCCG 1 cut(s) 327
Eco130I CCWWGG 4 cut(s) 27, 768, 786, 840
Eco147I AGGCCT 1 cut(s) 26
Eco24I GRGCYC 2 cut(s) 305, 781
Eco31I GGTCTC 2 cut(s) 150, 467
Eco47I GGWCC 4 cut(s) 318, 375, 547, 694
Eco52I CGGCCG 1 cut(s) 327
Eco72I CACGTG 1 cut(s) 192
Eco88I CYCGRG 2 cut(s) 470, 1082
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoO109I RGGNCCY 1 cut(s) 375
EcoRII CCWGG 2 cut(s) 455, 819
EcoT14I CCWWGG 4 cut(s) 27, 768, 786, 840
EcoT38I GRGCYC 2 cut(s) 305, 781
EgeI GGCGCC 1 cut(s) 441
EheI GGCGCC 1 cut(s) 441
ErhI CCWWGG 4 cut(s) 27, 768, 786, 840
FaeI CATG 4 cut(s) 142, 610, 790, 844
FaiI YATR 7 cut(s) 128, 140, 423, 608, 689, 788, 842
FaqI GGGAC 1 cut(s) 907
FatI CATG 4 cut(s) 138, 606, 786, 840
FauI CCCGC 2 cut(s) 173, 905
FblI GTMKAC 2 cut(s) 363, 825
Fnu4HI GCNGC 8 cut(s) 272, 511, 598, 624, 627, 712, 865, 1137
FokI GGATG 1 cut(s) 799
FriOI GRGCYC 2 cut(s) 305, 781
Fsp4HI GCNGC 8 cut(s) 272, 511, 598, 624, 627, 712, 865, 1137
FspAI RTGCGCAY 1 cut(s) 878
FspBI CTAG 1 cut(s) 38
FspI TGCGCA 1 cut(s) 878
GlaI GCGC 4 cut(s) 197, 441, 514, 878
GluI GCNGC 8 cut(s) 272, 511, 598, 624, 627, 712, 865, 1137
GsuI CTGGAG 3 cut(s) 129, 415, 439
HaeII RGCGCY 2 cut(s) 199, 443
HgaI GACGC 4 cut(s) 83, 106, 263, 473
HhaI GCGC 4 cut(s) 198, 442, 515, 879
Hin1I GRCGYC 5 cut(s) 75, 99, 117, 440, 465
Hin1II CATG 4 cut(s) 142, 610, 790, 844
Hin6I GCGC 4 cut(s) 196, 440, 513, 877
HinP1I GCGC 4 cut(s) 196, 440, 513, 877
HincII GTYRAC 2 cut(s) 364, 565
HindII GTYRAC 2 cut(s) 364, 565
HindIII AAGCTT 1 cut(s) 898
HinfI GANTC 6 cut(s) 10, 67, 81, 534, 795, 1001
HphI GGTGA 1 cut(s) 124
Hpy166II GTNNAC 4 cut(s) 364, 565, 592, 826
Hpy188I TCNGA 4 cut(s) 122, 208, 817, 1092
Hpy188III TCNNGA 5 cut(s) 18, 38, 71, 472, 722
Hpy8I GTNNAC 4 cut(s) 364, 565, 592, 826
HpyAV CCTTC 1 cut(s) 1135
HpyCH4III ACNGT 1 cut(s) 1120
HpyCH4IV ACGT 8 cut(s) 99, 191, 383, 518, 639, 873, 907, 948
HpyCH4V TGCA 3 cut(s) 556, 646, 1104
HpyF10VI GCNNNNNNNGC 8 cut(s) 83, 110, 521, 643, 652, 737, 797, 1038
HpyF3I CTNAG 2 cut(s) 304, 901
HpySE526I ACGT 8 cut(s) 99, 191, 383, 518, 639, 873, 907, 948
Hsp92I GRCGYC 5 cut(s) 75, 99, 117, 440, 465
Hsp92II CATG 4 cut(s) 142, 610, 790, 844
HspAI GCGC 4 cut(s) 196, 440, 513, 877
KasI GGCGCC 1 cut(s) 439
Kpn2I TCCGGA 2 cut(s) 17, 70
KroI GCCGGC 1 cut(s) 800
KroNI GCCGGC 1 cut(s) 802
KspI CCGCGG 1 cut(s) 624
Kzo9I GATC 3 cut(s) 208, 630, 1026
LguI GCTCTTC 1 cut(s) 149
LmnI GCTCC 5 cut(s) 110, 245, 861, 1117, 1144
Lsp1109I GCAGC 3 cut(s) 258, 876, 1123
LweI GCATC 1 cut(s) 1096
MabI ACCWGGT 1 cut(s) 819
MaeI CTAG 1 cut(s) 38
MaeII ACGT 8 cut(s) 99, 191, 383, 518, 639, 873, 907, 948
MalI GATC 3 cut(s) 210, 632, 1028
MbiI CCGCTC 1 cut(s) 240
MboI GATC 3 cut(s) 208, 630, 1026
MboII GAAGA 6 cut(s) 136, 442, 529, 737, 766, 1003
MhlI GDGCHC 4 cut(s) 305, 781, 1011, 1083
MlsI TGGCCA 1 cut(s) 605
MluCI AATT 2 cut(s) 411, 1042
MluI ACGCGT 1 cut(s) 738
MluNI TGGCCA 1 cut(s) 605
Mly113I GGCGCC 1 cut(s) 440
MlyI GAGTC 1 cut(s) 543
MmeI TCCRAC 2 cut(s) 100, 840
Mox20I TGGCCA 1 cut(s) 605
MreI CGCCGGCG 1 cut(s) 800
MroI TCCGGA 2 cut(s) 17, 70
MroNI GCCGGC 1 cut(s) 800
MscI TGGCCA 1 cut(s) 605
MseI TTAA 1 cut(s) 951
MslI CAYNNNNRTG 1 cut(s) 137
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 1 cut(s) 623
MspR9I CCNGG 6 cut(s) 264, 457, 531, 781, 821, 859
Mva1269I GAATGC 1 cut(s) 879
MvaI CCWGG 2 cut(s) 457, 821
MvnI CGCG 3 cut(s) 513, 623, 740
MwoI GCNNNNNNNGC 8 cut(s) 83, 110, 521, 643, 652, 737, 797, 1038
NaeI GCCGGC 1 cut(s) 802
NarI GGCGCC 1 cut(s) 440
NciI CCSGG 4 cut(s) 264, 531, 781, 859
NcoI CCATGG 2 cut(s) 786, 840
NdeII GATC 3 cut(s) 208, 630, 1026
NgoMIV GCCGGC 1 cut(s) 800
NlaIII CATG 4 cut(s) 142, 610, 790, 844
NlaIV GGNNCC 3 cut(s) 319, 441, 779
NsbI TGCGCA 1 cut(s) 878
NspV TTCGAA 1 cut(s) 540
PaeR7I CTCGAG 2 cut(s) 470, 1082
PceI AGGCCT 1 cut(s) 26
PciSI GCTCTTC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 3 cut(s) 283, 366, 813
PctI GAATGC 1 cut(s) 879
PdiI GCCGGC 1 cut(s) 802
PfeI GAWTC 5 cut(s) 10, 67, 81, 795, 1001
Pfl23II CGTACG 1 cut(s) 741
PflMI CCANNNNNTGG 2 cut(s) 721, 774
PfoI TCCNGGA 1 cut(s) 857
PinAI ACCGGT 1 cut(s) 544
PkrI GCNGC 8 cut(s) 273, 512, 599, 625, 628, 713, 866, 1138
PleI GAGTC 1 cut(s) 542
PluTI GGCGCC 1 cut(s) 443
PmaCI CACGTG 1 cut(s) 192
PmlI CACGTG 1 cut(s) 192
PpsI GAGTC 1 cut(s) 542
Ppu21I YACGTR 2 cut(s) 192, 874
PpuMI RGGWCCY 1 cut(s) 375
PshAI GACNNNNGTC 1 cut(s) 936
Psp1406I AACGTT 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 455, 819
PspCI CACGTG 1 cut(s) 192
PspGI CCWGG 2 cut(s) 455, 819
PspLI CGTACG 1 cut(s) 741
PspN4I GGNNCC 3 cut(s) 319, 441, 779
PspOMI GGGCCC 1 cut(s) 777
PspPI GGNCC 9 cut(s) 318, 375, 547, 694, 777, 778, 783, 790, 914
PspPPI RGGWCCY 1 cut(s) 375
PspXI VCTCGAGB 1 cut(s) 1082
PstNI CAGNNNCTG 1 cut(s) 721
RsaI GTAC 3 cut(s) 436, 737, 743
RsaNI GTAC 3 cut(s) 435, 736, 742
RseI CAYNNNNRTG 1 cut(s) 137
SacII CCGCGG 1 cut(s) 624
SalI GTCGAC 1 cut(s) 362
SapI GCTCTTC 1 cut(s) 149
SaqAI TTAA 1 cut(s) 951
SatI GCNGC 8 cut(s) 272, 511, 598, 624, 627, 712, 865, 1137
Sau3AI GATC 3 cut(s) 208, 630, 1026
Sau96I GGNCC 9 cut(s) 318, 375, 547, 694, 777, 778, 783, 790, 914
SchI GAGTC 1 cut(s) 543
ScrFI CCNGG 6 cut(s) 264, 457, 531, 781, 821, 859
SduI GDGCHC 4 cut(s) 305, 781, 1011, 1083
SexAI ACCWGGT 1 cut(s) 819
SfaNI GCATC 1 cut(s) 1096
SfoI GGCGCC 1 cut(s) 441
Sfr274I CTCGAG 2 cut(s) 470, 1082
Sfr303I CCGCGG 1 cut(s) 624
SfuI TTCGAA 1 cut(s) 540
SgrAI CRCCGGYG 1 cut(s) 800
SgrBI CCGCGG 1 cut(s) 624
SgrDI CGTCGACG 1 cut(s) 362
SinI GGWCC 4 cut(s) 318, 375, 547, 694
SlaI CTCGAG 2 cut(s) 470, 1082
SmiMI CAYNNNNRTG 1 cut(s) 137
SmlI CTYRAG 3 cut(s) 398, 470, 1082
SmoI CTYRAG 3 cut(s) 398, 470, 1082
Sse9I AATT 2 cut(s) 411, 1042
SseBI AGGCCT 1 cut(s) 26
SspDI GGCGCC 1 cut(s) 439
SspMI CTAG 1 cut(s) 38
StuI AGGCCT 1 cut(s) 26
StyD4I CCNGG 6 cut(s) 262, 455, 529, 779, 819, 857
StyI CCWWGG 4 cut(s) 27, 768, 786, 840
TaaI ACNGT 1 cut(s) 1120
TaiI ACGT 8 cut(s) 102, 194, 386, 521, 642, 876, 910, 951
TaqI TCGA 8 cut(s) 96, 286, 363, 471, 540, 681, 1056, 1083
TaqII GACCGA 1 cut(s) 491
TasI AATT 2 cut(s) 411, 1042
TauI GCSGC 5 cut(s) 513, 600, 626, 629, 714
TfiI GAWTC 5 cut(s) 10, 67, 81, 795, 1001
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 2 cut(s) 112, 1123
TseI GCWGC 3 cut(s) 271, 864, 1136
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 3 cut(s) 405, 550, 823
TspRI CASTG 2 cut(s) 112, 1123
Van91I CCANNNNNTGG 2 cut(s) 721, 774
VpaK11BI GGWCC 4 cut(s) 318, 375, 547, 694
XagI CCTNNNNNAGG 1 cut(s) 500
XbaI TCTAGA 1 cut(s) 37
XcmI CCANNNNNNNNNTGG 1 cut(s) 331
XhoI CTCGAG 2 cut(s) 470, 1082
XmiI GTMKAC 2 cut(s) 363, 825
XspI CTAG 1 cut(s) 38
ZraI GACGTC 1 cut(s) 100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.