AT3G54950

lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008401
Physical Location & Seq
Reverse (-)
20358896 .. 20361560
2665 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G54950.1

Sequence Viewer

Length: 1467 bp
ATGCATAGAGTACGCAATAAACCGGTTAAATCCACCGCTACTGCTTCCGTCAAGCATTTAATCAAACAGAGAGGCGGCGACGGAGCTACGGCGGCTTCCAAGTCGGCGAATGATTATAATAATAATGATAGTCTCTTGACGGATATGCAGGAACCAAGCATCGATACAGATAAACTCAGCTACGAGATTTTCTCTATTCTTGAGAGCAAATTTCTTTTCGGGTACGACGACTCGAAACCCGAACCCGCGAATTCGGTTGTTGCTGGGTCGATTAAGAACCAGAGAGGTAAAATCTGTATTCTGAGTATCGATGGAGGTGGAATGAGAGGGATCTTACCCGGTAAGGCTTTGGCTTATCTAGAACACGCTCTGAAATCTAAATCGGGTGACCCGAATGCCCGAATCGCCGATTACTTTGACGTAGCCGCCGGGTCCGGTATAGGTGGGATTTACACAGCCATGTTGTTCGGGTCAAGAGACGGTAACCGCCCCATCTTCAAAGCAGATGACACGTGGCAGTTCTTAACGAGAAACGCCAAGGGTCTTTACGGAGGAGCAGGAATCTTGAAACGGGTCTTGAGAACCGGGTCGGGTTGTTGTTCCGGGACGGCTAAGTTAAAGAAAGTGATGAAAGAGTCTTTCTCGGAGCTAACCTTAAAGGACACGCTTAAACCGGTGCTTATACCTTGCTACGACCTCAAAAGCTCTGGCCCGTTTCTGTTCTCACGGGCCGATGCGCTTGAGACGGACGGCTATGATTTTAGGCTCTCGGAGGTTTGTAGAGCGACTTGGGCTGAGCCGGGTGTGTTTGAGCCCGTGGAGATGAAGTCGGTTGATGGGCAGACCAAGTGTGTGGCTGTTGGTGGAGGATTAGCCATGAGCAATCCCACAGCGGCTGCAATCACGCATGTCCTGCACAACAAGCAGGAGTTTCCGTTTGTGCGAGGTGTTGAGGATTTGCTTGTGTTGTCTCTTGGTATGGGTCAGTTGCTTGATGTTAGCTATGAGTATGATCGGATTATCAAGTGGAAGGCTAAGCATTGGGCTCGCCCCGCAGCTCTTATTTCTAATGACGGTGCTGCTGACACTGTCGATCAAGCTGTGGCCATGGCTTTTGGTCACTGCCGCAGTAGTAACTATGTCCGGATTCAGGCGAATGGGTCGAACTTAGGACCTTGGAGTCCTAACATGGACACAGACCCTAGTGGGAGCAATGTGAATATGCTGATGGGAGTAGCAGAGGAGATGCTTAAACAAAAGAATGTTGAATCTGTTTTGTTTGGAGGTAAAAGAATCGATGAACAGAGCAACTTCGAGAAGCTTGACTGGTTAGCTGGGGAACTTGTTCTCGAGCATCAAAGGAGAAATAGCAGAATCGCTCCAACCGTAGCGTTCAAGCAATCCGTGCATAGAGCAGATCAAAAGACAAGTGATAAAGATATCGGTGTGACCGCAAGAGAACGATGA

Protein Analysis

488

Amino Acids

53.1

Weight (kDa)

8.63

Isoelectric Point (pI)

28.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 101 - 301 3.3e-18 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 117
AasI GACNNNNNNGTC 1 cut(s) 1179
AccII CGCG 1 cut(s) 248
AccIII TCCGGA 1 cut(s) 1143
AclWI GGATC 1 cut(s) 338
AcoI YGGCCR 1 cut(s) 1104
AcsI RAATTY 2 cut(s) 209, 250
AcvI CACGTG 1 cut(s) 513
AfaI GTAC 2 cut(s) 12, 224
AfiI CCNNNNNNNGG 1 cut(s) 1150
AflIII ACRYGT 1 cut(s) 510
AgeI ACCGGT 2 cut(s) 22, 673
AgsI TTSAA 4 cut(s) 499, 568, 1268, 1396
AluBI AGCT 9 cut(s) 86, 180, 649, 705, 1002, 1058, 1100, 1321, 1334
AluI AGCT 9 cut(s) 86, 180, 649, 705, 1002, 1058, 1100, 1321, 1334
Alw26I GTCTC 4 cut(s) 137, 471, 737, 975
AlwI GGATC 1 cut(s) 338
AlwNI CAGNNNCTG 1 cut(s) 896
Ama87I CYCGRG 1 cut(s) 1349
Aor13HI TCCGGA 1 cut(s) 1143
AoxI GGCC 3 cut(s) 709, 729, 1104
ApeKI GCWGC 3 cut(s) 896, 1055, 1079
ApoI RAATTY 2 cut(s) 209, 250
AsiGI ACCGGT 2 cut(s) 22, 673
Asp700I GAANNNNTTC 1 cut(s) 1344
AspLEI GCGC 1 cut(s) 739
AspS9I GGNCC 4 cut(s) 432, 710, 729, 1172
AsuC2I CCSGG 5 cut(s) 339, 430, 586, 604, 801
AsuHPI GGTGA 1 cut(s) 398
AvaI CYCGRG 1 cut(s) 1349
AvaII GGWCC 2 cut(s) 432, 1172
BalI TGGCCA 1 cut(s) 1106
BanII GRGCYC 2 cut(s) 816, 1048
BbrPI CACGTG 1 cut(s) 513
BbvI GCAGC 3 cut(s) 883, 1066, 1067
BccI CCATC 4 cut(s) 305, 500, 830, 1222
BceAI ACGGC 3 cut(s) 105, 624, 766
BcgI CGANNNNNNTGC 2 cut(s) 1028, 1062
BcnI CCSGG 5 cut(s) 339, 430, 586, 604, 801
BcoDI GTCTC 4 cut(s) 137, 471, 737, 975
BfaI CTAG 2 cut(s) 359, 1203
BisI GCNGC 8 cut(s) 76, 93, 426, 894, 897, 1056, 1080, 1126
BlpI GCTNAGC 2 cut(s) 795, 1035
BlsI GCNGC 8 cut(s) 77, 94, 427, 895, 898, 1057, 1081, 1127
Bme1390I CCNGG 5 cut(s) 339, 430, 586, 604, 801
Bme18I GGWCC 2 cut(s) 432, 1172
BmeT110I CYCGRG 1 cut(s) 1349
BmgT120I GGNCC 4 cut(s) 432, 710, 729, 1172
BmiI GGNNCC 2 cut(s) 153, 433
BmrFI CCNGG 5 cut(s) 339, 430, 586, 604, 801
BmsI GCATC 4 cut(s) 168, 724, 1236, 1363
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 626, 658
Bpu1102I GCTNAGC 2 cut(s) 795, 1035
BpuEI CTTGAG 3 cut(s) 221, 598, 761
BpuMI CCSGG 5 cut(s) 339, 430, 586, 604, 801
Bsa29I ATCGAT 3 cut(s) 162, 309, 1296
BsaAI YACGTR 1 cut(s) 513
BsaJI CCNNGG 4 cut(s) 537, 816, 1107, 1175
BsaWI WCCGGW 4 cut(s) 22, 434, 673, 1143
BsaXI ACNNNNNCTCC 4 cut(s) 812, 842, 920, 950
Bsc4I CCNNNNNNNGG 1 cut(s) 1150
Bse118I RCCGGY 2 cut(s) 22, 673
Bse1I ACTGG 1 cut(s) 1331
Bse3DI GCAATG 1 cut(s) 1219
BseAI TCCGGA 1 cut(s) 1143
BseCI ATCGAT 3 cut(s) 162, 309, 1296
BseDI CCNNGG 4 cut(s) 537, 816, 1107, 1175
BseLI CCNNNNNNNGG 1 cut(s) 1150
BseMI GCAATG 1 cut(s) 1219
BseMII CTCAG 3 cut(s) 190, 293, 786
BseNI ACTGG 1 cut(s) 1331
BseRI GAGGAG 2 cut(s) 567, 1256
BseXI GCAGC 3 cut(s) 883, 1066, 1067
BseYI CCCAGC 2 cut(s) 263, 1334
BsgI GTGCAG 1 cut(s) 899
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 3 cut(s) 711, 731, 1106
BshTI ACCGGT 2 cut(s) 22, 673
BshVI ATCGAT 3 cut(s) 162, 309, 1296
BsiHKCI CYCGRG 1 cut(s) 1349
BsiSI CCGG 9 cut(s) 23, 339, 429, 435, 585, 603, 674, 800, 1144
BslFI GGGAC 1 cut(s) 619
BslI CCNNNNNNNGG 1 cut(s) 1150
BsmAI GTCTC 4 cut(s) 137, 471, 737, 975
BsmBI CGTCTC 2 cut(s) 471, 737
BsmFI GGGAC 1 cut(s) 619
BsmI GAATGC 1 cut(s) 400
BsnI GGCC 3 cut(s) 711, 731, 1106
BsoBI CYCGRG 1 cut(s) 1349
Bsp1286I GDGCHC 2 cut(s) 816, 1048
Bsp13I TCCGGA 1 cut(s) 1143
Bsp143I GATC 4 cut(s) 330, 1012, 1093, 1417
Bsp1720I GCTNAGC 2 cut(s) 795, 1035
Bsp19I CCATGG 1 cut(s) 1107
BspANI GGCC 3 cut(s) 711, 731, 1106
BspCNI CTCAG 3 cut(s) 189, 294, 787
BspDI ATCGAT 3 cut(s) 162, 309, 1296
BspEI TCCGGA 1 cut(s) 1143
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 2 cut(s) 153, 433
BspPI GGATC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 1219
BsrFI RCCGGY 2 cut(s) 22, 673
BsrI ACTGG 1 cut(s) 1331
BssAI RCCGGY 2 cut(s) 22, 673
BssECI CCNNGG 4 cut(s) 537, 816, 1107, 1175
BssMI GATC 4 cut(s) 330, 1012, 1093, 1417
BssT1I CCWWGG 3 cut(s) 537, 1107, 1175
Bst4CI ACNGT 4 cut(s) 482, 1076, 1090, 1387
BstAPI GCANNNNNTGC 2 cut(s) 913, 1405
BstBAI YACGTR 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 1048
BstDEI CTNAG 6 cut(s) 176, 302, 612, 795, 1035, 1168
BstDSI CCRYGG 2 cut(s) 816, 1107
BstEII GGTNACC 2 cut(s) 386, 482
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 739
BstKTI GATC 4 cut(s) 333, 1015, 1096, 1420
BstMAI GTCTC 4 cut(s) 137, 471, 737, 975
BstMBI GATC 4 cut(s) 330, 1012, 1093, 1417
BstMWI GCNNNNNNNGC 7 cut(s) 92, 404, 791, 913, 922, 1052, 1405
BstNSI RCATGY 1 cut(s) 911
BstPI GGTNACC 2 cut(s) 386, 482
BstSCI CCNGG 5 cut(s) 337, 428, 584, 602, 799
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 883, 1066, 1067
BstX2I RGATCY 1 cut(s) 330
BstXI CCANNNNNNTGG 1 cut(s) 853
BstYI RGATCY 1 cut(s) 330
Bsu15I ATCGAT 3 cut(s) 162, 309, 1296
BsuRI GGCC 3 cut(s) 711, 731, 1106
BsuTUI ATCGAT 3 cut(s) 162, 309, 1296
BtgI CCRYGG 2 cut(s) 816, 1107
BtsI GCAGTG 1 cut(s) 1120
BtsIMutI CAGTG 2 cut(s) 1086, 1120
Cac8I GCNNGC 1 cut(s) 1048
CaiI CAGNNNCTG 1 cut(s) 896
CfoI GCGC 1 cut(s) 739
Cfr10I RCCGGY 2 cut(s) 22, 673
Cfr13I GGNCC 4 cut(s) 432, 710, 729, 1172
ClaI ATCGAT 3 cut(s) 162, 309, 1296
Csp6I GTAC 2 cut(s) 11, 223
CspAI ACCGGT 2 cut(s) 22, 673
CviAII CATG 5 cut(s) 460, 877, 908, 1108, 1189
CviQI GTAC 2 cut(s) 11, 223
DdeI CTNAG 6 cut(s) 176, 302, 612, 795, 1035, 1168
DpnI GATC 4 cut(s) 332, 1014, 1095, 1419
DpnII GATC 4 cut(s) 330, 1012, 1093, 1417
DrdI GACNNNNNNGTC 1 cut(s) 1179
DseDI GACNNNNNNGTC 1 cut(s) 1179
EaeI YGGCCR 1 cut(s) 1104
Eco130I CCWWGG 3 cut(s) 537, 1107, 1175
Eco24I GRGCYC 2 cut(s) 816, 1048
Eco32I GATATC 1 cut(s) 1441
Eco47I GGWCC 2 cut(s) 432, 1172
Eco72I CACGTG 1 cut(s) 513
Eco88I CYCGRG 1 cut(s) 1349
Eco91I GGTNACC 2 cut(s) 386, 482
EcoO109I RGGNCCY 1 cut(s) 1172
EcoO65I GGTNACC 2 cut(s) 386, 482
EcoRI GAATTC 1 cut(s) 250
EcoRV GATATC 1 cut(s) 1441
EcoT14I CCWWGG 3 cut(s) 537, 1107, 1175
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 2 cut(s) 816, 1048
ErhI CCWWGG 3 cut(s) 537, 1107, 1175
Esp3I CGTCTC 2 cut(s) 471, 737
FaeI CATG 5 cut(s) 463, 880, 911, 1111, 1192
FaqI GGGAC 1 cut(s) 619
FatI CATG 5 cut(s) 459, 876, 907, 1107, 1188
FauI CCCGC 2 cut(s) 253, 1060
Fnu4HI GCNGC 8 cut(s) 76, 93, 426, 894, 897, 1056, 1080, 1126
FriOI GRGCYC 2 cut(s) 816, 1048
Fsp4HI GCNGC 8 cut(s) 76, 93, 426, 894, 897, 1056, 1080, 1126
FspBI CTAG 2 cut(s) 359, 1203
GlaI GCGC 1 cut(s) 738
GluI GCNGC 8 cut(s) 76, 93, 426, 894, 897, 1056, 1080, 1126
GsaI CCCAGC 2 cut(s) 267, 1338
HaeIII GGCC 3 cut(s) 711, 731, 1106
HapII CCGG 9 cut(s) 23, 339, 429, 435, 585, 603, 674, 800, 1144
HhaI GCGC 1 cut(s) 739
Hin1II CATG 5 cut(s) 463, 880, 911, 1111, 1192
Hin6I GCGC 1 cut(s) 737
HinP1I GCGC 1 cut(s) 737
HindIII AAGCTT 1 cut(s) 1319
HinfI GANTC 9 cut(s) 230, 402, 561, 635, 1147, 1180, 1268, 1293, 1374
HpaII CCGG 9 cut(s) 23, 339, 429, 435, 585, 603, 674, 800, 1144
HphI GGTGA 1 cut(s) 398
Hpy188I TCNGA 5 cut(s) 303, 372, 646, 772, 1017
Hpy188III TCNNGA 9 cut(s) 136, 200, 359, 474, 565, 577, 1144, 1315, 1349
Hpy99I CGWCG 2 cut(s) 83, 230
HpyAV CCTTC 1 cut(s) 1024
HpyCH4III ACNGT 4 cut(s) 482, 1076, 1090, 1387
HpyCH4IV ACGT 2 cut(s) 420, 512
HpyCH4V TGCA 5 cut(s) 4, 148, 899, 916, 1408
HpyF10VI GCNNNNNNNGC 7 cut(s) 92, 404, 791, 913, 922, 1052, 1405
HpyF3I CTNAG 6 cut(s) 176, 302, 612, 795, 1035, 1168
HpySE526I ACGT 2 cut(s) 420, 512
Hsp92II CATG 5 cut(s) 463, 880, 911, 1111, 1192
HspAI GCGC 1 cut(s) 737
Kpn2I TCCGGA 1 cut(s) 1143
Kzo9I GATC 4 cut(s) 330, 1012, 1093, 1417
LmnI GCTCC 5 cut(s) 83, 554, 646, 1209, 1384
Lsp1109I GCAGC 3 cut(s) 883, 1066, 1067
LweI GCATC 4 cut(s) 168, 724, 1236, 1363
MaeI CTAG 2 cut(s) 359, 1203
MaeII ACGT 2 cut(s) 420, 512
MaeIII GTNAC 5 cut(s) 386, 482, 1118, 1133, 1447
MalI GATC 4 cut(s) 332, 1014, 1095, 1419
MboI GATC 4 cut(s) 330, 1012, 1093, 1417
MboII GAAGA 1 cut(s) 487
MflI RGATCY 1 cut(s) 330
MhlI GDGCHC 2 cut(s) 816, 1048
MlsI TGGCCA 1 cut(s) 1106
MluCI AATT 2 cut(s) 209, 250
MluNI TGGCCA 1 cut(s) 1106
MlyI GAGTC 3 cut(s) 224, 644, 1189
MmeI TCCRAC 1 cut(s) 1406
Mox20I TGGCCA 1 cut(s) 1106
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 1143
MroXI GAANNNNTTC 1 cut(s) 1344
MscI TGGCCA 1 cut(s) 1106
MseI TTAA 8 cut(s) 27, 59, 273, 524, 617, 656, 669, 1251
MslI CAYNNNNRTG 1 cut(s) 458
Msp20I TGGCCA 1 cut(s) 1106
MspA1I CMGCKG 1 cut(s) 893
MspI CCGG 9 cut(s) 23, 339, 429, 435, 585, 603, 674, 800, 1144
MspR9I CCNGG 5 cut(s) 339, 430, 586, 604, 801
Mva1269I GAATGC 1 cut(s) 400
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 7 cut(s) 92, 404, 791, 913, 922, 1052, 1405
NciI CCSGG 5 cut(s) 339, 430, 586, 604, 801
NcoI CCATGG 1 cut(s) 1107
NdeII GATC 4 cut(s) 330, 1012, 1093, 1417
NlaIII CATG 5 cut(s) 463, 880, 911, 1111, 1192
NlaIV GGNNCC 2 cut(s) 153, 433
NmuCI GTSAC 3 cut(s) 386, 1118, 1447
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 911
PaeR7I CTCGAG 1 cut(s) 1349
PcsI WCGNNNNNNNCGW 3 cut(s) 225, 389, 1383
PctI GAATGC 1 cut(s) 400
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 6 cut(s) 402, 561, 1147, 1268, 1293, 1374
PflFI GACNNNGTC 1 cut(s) 1088
PfoI TCCNGGA 1 cut(s) 602
PinAI ACCGGT 2 cut(s) 22, 673
PkrI GCNGC 8 cut(s) 77, 94, 427, 895, 898, 1057, 1081, 1127
PleI GAGTC 3 cut(s) 224, 643, 1188
PmaCI CACGTG 1 cut(s) 513
PmlI CACGTG 1 cut(s) 513
PpsI GAGTC 3 cut(s) 224, 643, 1188
Ppu21I YACGTR 1 cut(s) 513
PpuMI RGGWCCY 1 cut(s) 1172
PsiI TTATAA 1 cut(s) 117
Psp5II RGGWCCY 1 cut(s) 1172
PspCI CACGTG 1 cut(s) 513
PspEI GGTNACC 2 cut(s) 386, 482
PspFI CCCAGC 2 cut(s) 263, 1334
PspN4I GGNNCC 2 cut(s) 153, 433
PspPI GGNCC 4 cut(s) 432, 710, 729, 1172
PspPPI RGGWCCY 1 cut(s) 1172
PstNI CAGNNNCTG 1 cut(s) 896
PsuI RGATCY 1 cut(s) 330
PsyI GACNNNGTC 1 cut(s) 1088
RsaI GTAC 2 cut(s) 12, 224
RsaNI GTAC 2 cut(s) 11, 223
RseI CAYNNNNRTG 1 cut(s) 458
SaqAI TTAA 8 cut(s) 27, 59, 273, 524, 617, 656, 669, 1251
SatI GCNGC 8 cut(s) 76, 93, 426, 894, 897, 1056, 1080, 1126
Sau3AI GATC 4 cut(s) 330, 1012, 1093, 1417
Sau96I GGNCC 4 cut(s) 432, 710, 729, 1172
SchI GAGTC 3 cut(s) 224, 644, 1189
ScrFI CCNGG 5 cut(s) 339, 430, 586, 604, 801
SduI GDGCHC 2 cut(s) 816, 1048
SfaNI GCATC 4 cut(s) 168, 724, 1236, 1363
Sfr274I CTCGAG 1 cut(s) 1349
SinI GGWCC 2 cut(s) 432, 1172
SlaI CTCGAG 1 cut(s) 1349
SmiMI CAYNNNNRTG 1 cut(s) 458
SmlI CTYRAG 4 cut(s) 200, 577, 740, 1349
SmoI CTYRAG 4 cut(s) 200, 577, 740, 1349
Sse9I AATT 2 cut(s) 209, 250
SspMI CTAG 2 cut(s) 359, 1203
StyD4I CCNGG 5 cut(s) 337, 428, 584, 602, 799
StyI CCWWGG 3 cut(s) 537, 1107, 1175
TaaI ACNGT 4 cut(s) 482, 1076, 1090, 1387
TaiI ACGT 2 cut(s) 423, 515
TaqI TCGA 9 cut(s) 162, 233, 269, 309, 1092, 1163, 1296, 1314, 1350
TasI AATT 2 cut(s) 209, 250
TauI GCSGC 5 cut(s) 78, 95, 428, 896, 1128
TfiI GAWTC 6 cut(s) 402, 561, 1147, 1268, 1293, 1374
Tru1I TTAA 8 cut(s) 27, 59, 273, 524, 617, 656, 669, 1251
Tru9I TTAA 8 cut(s) 27, 59, 273, 524, 617, 656, 669, 1251
TscAI CASTG 2 cut(s) 1093, 1127
TseFI GTSAC 3 cut(s) 386, 1118, 1447
TseI GCWGC 3 cut(s) 896, 1055, 1079
Tsp45I GTSAC 3 cut(s) 386, 1118, 1447
TspDTI ATGAA 3 cut(s) 644, 839, 1314
TspGWI ACGGA 7 cut(s) 37, 96, 155, 564, 761, 924, 1393
TspRI CASTG 2 cut(s) 1093, 1127
Tth111I GACNNNGTC 1 cut(s) 1088
VpaK11BI GGWCC 2 cut(s) 432, 1172
XapI RAATTY 2 cut(s) 209, 250
XbaI TCTAGA 1 cut(s) 358
XceI RCATGY 1 cut(s) 911
XhoI CTCGAG 1 cut(s) 1349
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 2 cut(s) 359, 1203
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.