Rorug01G0307300

Lipolytic acyl hydrolase (LAH)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008401
Physical Location & Seq
Forward (+)
42435840 .. 42437150
1311 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0307300.1

Sequence Viewer

Length: 1311 bp
ATGGATGAAGTTGCGGCCTCGTTTGCGGCATCATTGGCCCTTGCAACCAATGAGGTTGTAGATCTCTCAAGATCTGCAAACTCTGCGGAACGCCGCGGGAAGCAGTCCTATCTGCTAGCCCGCCCACTTGCAGCCAAACCAGCCCCACTACAAGACCTCCGGCGTTTCTTTCGTCGTGTATGGGTGCTTGACAGAGATTTTAAGATTCAAGAGCGAGGAGAAAACCGTTTTGTCATAGCTTTTGACCTCAAACGGGATCGCAACAAGGTTTTACGTGGAGGATCGTGGCGCTTCAACCAAGCCCCGGTCGTGATGCAAGAGTATAATGGGATTGCACCGCCGCTCTCGGTGGCCTTGAATGCATTATTTTTCTGGGTACGTATAACTGGTATACCACCACTCTTTAAGGACAAAGAAACTATTAGAGATATTGCTGCTATTGCGGGCAAGGTCTTGGAGTTAGATGATAAAATTTTTGACAATACTGGTAAGATCAGGGTTAGGGTGTCCCGGGAGCTTTCCAAGCCTTTTGATCTGAAAAAGAAAGTCAAACTTGCACCGGGTGTTGAAGCAGAGATCAGTTTCTTCTTTGAAAACCTTGTGGGTAAGTGTAACTATTGTGACTTGATTTTCCATGAGCATGGTGTTTGTCCTGCAGCAGATGGCAGGGCTCTTTCCCGTATGGAGCCAAACCCGGTGCAACGGAAATTGAATATCACGGGCCGTTCTCTGAGTTTTATGCAAAACCGTTTTGAGAATGGCATGTTCAAGTTCCATGGACTTCAAACCCCTATATTACCGTCAGTGGCACGCTCTCTTTTTCACTCAAAAGAGCATGGTGGCCGTAAACCTGTGGTGTTGTGGGGATCGGATAATAGATCGGCCTCCAGCAGGGATGAGGAGAATACCATCTTGGCTTTGGTTCAGGTGGAGACAGCTGCGTCAACTAAGCGTGATTGGCAGCCCTCTGATTATCGCTCCCCAACAAAGCTAAAATTACTCTTGCCTGAGTTGTTTAATTCCTCGGGATGCAATGAGGCTCCCTCCAAGAAACTGAAGATGCCGAAGTTCAGCTCTAAATTTAATGCGAAATCCCTGGGGCTGATTGAAGCTCCGGGTAGCATCCTTGTTCCAAAGTCTGAGGCTGACAGTTCTGGGACCAAAAAGAAACGAGGCAGGCCCTTGGGATCAAAGAACAAAAATCCACCGAAGCCGAAAAATGTTGCTGCTGCACAGGAAGCTTGCTTGGCTTTTTTAACAAAACCTCCTAGCCCTAGTGTTAAGGGCAAGAAGAAAATATATAGTTTTTAG

Protein Analysis

436

Amino Acids

48.65

Weight (kDa)

10.06

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 36 - 109 8.5e-12 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 940
AccBSI CCGCTC 1 cut(s) 343
AccI GTMKAC 1 cut(s) 391
AccII CGCG 1 cut(s) 96
AciI CCGC 9 cut(s) 14, 26, 86, 94, 96, 121, 338, 341, 443
AclWI GGATC 4 cut(s) 264, 289, 874, 1195
AcoI YGGCCR 1 cut(s) 841
AcsI RAATTY 2 cut(s) 471, 1079
AcuI CTGAAG 1 cut(s) 1076
AdeI CACNNNGTG 1 cut(s) 563
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 3 cut(s) 253, 304, 891
AgsI TTSAA 9 cut(s) 209, 295, 358, 569, 593, 712, 769, 785, 1109
AjnI CCWGG 1 cut(s) 1095
AjuI GAANNNNNNNTTGG 2 cut(s) 896, 928
AluBI AGCT 7 cut(s) 239, 517, 938, 991, 1074, 1112, 1241
AluI AGCT 7 cut(s) 239, 517, 938, 991, 1074, 1112, 1241
Alw26I GTCTC 1 cut(s) 926
AlwI GGATC 4 cut(s) 264, 289, 874, 1195
Ama87I CYCGRG 2 cut(s) 510, 1024
AoxI GGCC 7 cut(s) 15, 36, 351, 721, 841, 882, 1178
ApeKI GCWGC 7 cut(s) 131, 434, 656, 938, 961, 1226, 1229
ApoI RAATTY 2 cut(s) 471, 1079
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 4 cut(s) 37, 721, 1158, 1179
AsuC2I CCSGG 6 cut(s) 305, 511, 512, 561, 695, 1116
AsuNHI GCTAGC 1 cut(s) 115
AvaI CYCGRG 2 cut(s) 510, 1024
AvaII GGWCC 1 cut(s) 1158
BanII GRGCYC 1 cut(s) 673
BbvI GCAGC 7 cut(s) 143, 421, 668, 925, 973, 1213, 1216
BccI CCATC 2 cut(s) 656, 917
BceAI ACGGC 2 cut(s) 708, 828
BciT130I CCWGG 1 cut(s) 1097
BcnI CCSGG 6 cut(s) 305, 511, 512, 561, 695, 1116
BcoDI GTCTC 1 cut(s) 926
BfaI CTAG 3 cut(s) 116, 1269, 1275
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 292
BglII AGATCT 2 cut(s) 61, 71
Bme1390I CCNGG 7 cut(s) 305, 511, 512, 561, 695, 1097, 1116
Bme18I GGWCC 1 cut(s) 1158
BmeT110I CYCGRG 2 cut(s) 510, 1024
BmgT120I GGNCC 4 cut(s) 37, 721, 1158, 1179
BmiI GGNNCC 3 cut(s) 687, 1041, 1159
BmrFI CCNGG 7 cut(s) 305, 511, 512, 561, 695, 1097, 1116
BmsI GCATC 5 cut(s) 38, 303, 1019, 1050, 1131
BmtI GCTAGC 1 cut(s) 119
BplI GAGNNNNNCTC 2 cut(s) 1028, 1060
BpmI CTGGAG 1 cut(s) 871
BpuEI CTTGAG 1 cut(s) 52
BpuMI CCSGG 6 cut(s) 305, 511, 512, 561, 695, 1116
BsaAI YACGTR 2 cut(s) 275, 380
BsaJI CCNNGG 8 cut(s) 94, 303, 510, 775, 1023, 1095, 1096, 1182
BsaXI ACNNNNNCTCC 4 cut(s) 141, 171, 1249, 1279
Bsc4I CCNNNNNNNGG 3 cut(s) 253, 304, 891
Bse1I ACTGG 2 cut(s) 391, 490
Bse3DI GCAATG 1 cut(s) 1039
BseBI CCWGG 1 cut(s) 1097
BseDI CCNNGG 8 cut(s) 94, 303, 510, 775, 1023, 1095, 1096, 1182
BseGI GGATG 4 cut(s) 10, 901, 1034, 1122
BseLI CCNNNNNNNGG 3 cut(s) 253, 304, 891
BseMI GCAATG 1 cut(s) 1039
BseMII CTCAG 3 cut(s) 722, 999, 1131
BseNI ACTGG 2 cut(s) 391, 490
BseRI GAGGAG 2 cut(s) 231, 914
BseXI GCAGC 7 cut(s) 143, 421, 668, 925, 973, 1213, 1216
BsgI GTGCAG 1 cut(s) 1215
Bsh1236I CGCG 1 cut(s) 96
Bsh1285I CGRYCG 1 cut(s) 309
BshFI GGCC 7 cut(s) 17, 38, 353, 723, 843, 884, 1180
BsiEI CGRYCG 1 cut(s) 309
BsiHKCI CYCGRG 2 cut(s) 510, 1024
BsiSI CCGG 6 cut(s) 160, 305, 511, 560, 695, 1115
BslFI GGGAC 2 cut(s) 493, 1171
BslI CCNNNNNNNGG 3 cut(s) 253, 304, 891
BsmAI GTCTC 1 cut(s) 926
BsmFI GGGAC 2 cut(s) 493, 1171
BsmI GAATGC 1 cut(s) 364
BsnI GGCC 7 cut(s) 17, 38, 353, 723, 843, 884, 1180
BsoBI CYCGRG 2 cut(s) 510, 1024
Bsp1286I GDGCHC 1 cut(s) 673
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 9 cut(s) 14, 26, 86, 94, 96, 121, 338, 341, 443
BspANI GGCC 7 cut(s) 17, 38, 353, 723, 843, 884, 1180
BspCNI CTCAG 3 cut(s) 723, 1000, 1132
BspFNI CGCG 1 cut(s) 96
BspLI GGNNCC 3 cut(s) 687, 1041, 1159
BspMAI CTGCAG 1 cut(s) 658
BspOI GCTAGC 1 cut(s) 119
BspPI GGATC 4 cut(s) 264, 289, 874, 1195
BsrBI CCGCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 1039
BsrI ACTGG 2 cut(s) 391, 490
BssECI CCNNGG 8 cut(s) 94, 303, 510, 775, 1023, 1095, 1096, 1182
BssNAI GTATAC 1 cut(s) 392
BssT1I CCWWGG 2 cut(s) 775, 1182
Bst1107I GTATAC 1 cut(s) 392
Bst2UI CCWGG 1 cut(s) 1097
Bst4CI ACNGT 4 cut(s) 227, 749, 801, 1151
BstAPI GCANNNNNTGC 1 cut(s) 83
BstBAI YACGTR 2 cut(s) 275, 380
BstC8I GCNNGC 6 cut(s) 117, 121, 445, 811, 1178, 1243
BstDEI CTNAG 4 cut(s) 731, 948, 1008, 1140
BstDSI CCRYGG 2 cut(s) 94, 775
BstENI CCTNNNNNAGG 1 cut(s) 889
BstF5I GGATG 4 cut(s) 10, 901, 1034, 1122
BstFNI CGCG 1 cut(s) 96
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 1 cut(s) 291
BstMAI GTCTC 1 cut(s) 926
BstMCI CGRYCG 1 cut(s) 309
BstNI CCWGG 1 cut(s) 1097
BstNSI RCATGY 1 cut(s) 766
BstSCI CCNGG 7 cut(s) 303, 509, 510, 559, 693, 1095, 1114
BstSFI CTRYAG 1 cut(s) 654
BstSNI TACGTA 1 cut(s) 380
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 7 cut(s) 143, 421, 668, 925, 973, 1213, 1216
BstX2I RGATCY 2 cut(s) 61, 71
BstXI CCANNNNNNTGG 1 cut(s) 641
BstYI RGATCY 2 cut(s) 61, 71
BstZ17I GTATAC 1 cut(s) 392
BsuRI GGCC 7 cut(s) 17, 38, 353, 723, 843, 884, 1180
BtgI CCRYGG 2 cut(s) 94, 775
BtsCI GGATG 4 cut(s) 10, 901, 1034, 1122
BtsIMutI CAGTG 1 cut(s) 810
Cac8I GCNNGC 6 cut(s) 117, 121, 445, 811, 1178, 1243
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 4 cut(s) 37, 721, 1158, 1179
Cfr42I CCGCGG 1 cut(s) 97
Cfr9I CCCGGG 1 cut(s) 510
CseI GACGC 1 cut(s) 930
Csp6I GTAC 1 cut(s) 377
CviAII CATG 5 cut(s) 635, 641, 763, 776, 836
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 4 cut(s) 731, 948, 1008, 1140
DraIII CACNNNGTG 1 cut(s) 563
DrdI GACNNNNNNGTC 1 cut(s) 940
DseDI GACNNNNNNGTC 1 cut(s) 940
EaeI YGGCCR 1 cut(s) 841
Eco105I TACGTA 1 cut(s) 380
Eco130I CCWWGG 2 cut(s) 775, 1182
Eco24I GRGCYC 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 1158
Eco57I CTGAAG 1 cut(s) 1076
Eco88I CYCGRG 2 cut(s) 510, 1024
EcoNI CCTNNNNNAGG 1 cut(s) 889
EcoO109I RGGNCCY 1 cut(s) 1179
EcoRII CCWGG 1 cut(s) 1095
EcoT14I CCWWGG 2 cut(s) 775, 1182
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 673
ErhI CCWWGG 2 cut(s) 775, 1182
FaeI CATG 5 cut(s) 638, 644, 766, 779, 839
FalI AAGNNNNNCTT 2 cut(s) 537, 569
FaqI GGGAC 2 cut(s) 493, 1171
FatI CATG 5 cut(s) 634, 640, 762, 775, 835
FauI CCCGC 3 cut(s) 89, 128, 436
FblI GTMKAC 1 cut(s) 391
FokI GGATG 4 cut(s) 17, 908, 1041, 1109
FriOI GRGCYC 1 cut(s) 673
FspBI CTAG 3 cut(s) 116, 1269, 1275
GlaI GCGC 1 cut(s) 290
GsuI CTGGAG 1 cut(s) 871
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 7 cut(s) 17, 38, 353, 723, 843, 884, 1180
HapII CCGG 6 cut(s) 160, 305, 511, 560, 695, 1115
HgaI GACGC 1 cut(s) 930
HhaI GCGC 1 cut(s) 291
Hin1II CATG 5 cut(s) 638, 644, 766, 779, 839
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 1 cut(s) 945
HindII GTYRAC 1 cut(s) 945
HindIII AAGCTT 1 cut(s) 1239
HinfI GANTC 1 cut(s) 205
HpaII CCGG 6 cut(s) 160, 305, 511, 560, 695, 1115
Hpy166II GTNNAC 3 cut(s) 392, 848, 945
Hpy188I TCNGA 5 cut(s) 537, 732, 871, 970, 1141
Hpy188III TCNNGA 4 cut(s) 69, 209, 310, 1026
Hpy8I GTNNAC 3 cut(s) 392, 848, 945
Hpy99I CGWCG 1 cut(s) 177
HpyCH4III ACNGT 4 cut(s) 227, 749, 801, 1151
HpyCH4IV ACGT 2 cut(s) 274, 379
HpyF3I CTNAG 4 cut(s) 731, 948, 1008, 1140
HpySE526I ACGT 2 cut(s) 274, 379
Hsp92II CATG 5 cut(s) 638, 644, 766, 779, 839
HspAI GCGC 1 cut(s) 289
KspI CCGCGG 1 cut(s) 97
LmnI GCTCC 5 cut(s) 514, 685, 983, 1045, 1117
Lsp1109I GCAGC 7 cut(s) 143, 421, 668, 925, 973, 1213, 1216
LweI GCATC 5 cut(s) 38, 303, 1019, 1050, 1131
MaeI CTAG 3 cut(s) 116, 1269, 1275
MaeII ACGT 2 cut(s) 274, 379
MaeIII GTNAC 2 cut(s) 611, 620
MbiI CCGCTC 1 cut(s) 343
MboII GAAGA 3 cut(s) 577, 1069, 1303
MflI RGATCY 2 cut(s) 61, 71
MhlI GDGCHC 1 cut(s) 673
MluCI AATT 5 cut(s) 471, 707, 995, 1018, 1079
Mph1103I ATGCAT 1 cut(s) 364
MseI TTAA 6 cut(s) 201, 405, 1017, 1083, 1256, 1281
MslI CAYNNNNRTG 1 cut(s) 639
MspA1I CMGCKG 2 cut(s) 96, 938
MspI CCGG 6 cut(s) 160, 305, 511, 560, 695, 1115
MspR9I CCNGG 7 cut(s) 305, 511, 512, 561, 695, 1097, 1116
Mva1269I GAATGC 1 cut(s) 364
MvaI CCWGG 1 cut(s) 1097
MvnI CGCG 1 cut(s) 96
NciI CCSGG 6 cut(s) 305, 511, 512, 561, 695, 1116
NcoI CCATGG 1 cut(s) 775
NheI GCTAGC 1 cut(s) 115
NlaIII CATG 5 cut(s) 638, 644, 766, 779, 839
NlaIV GGNNCC 3 cut(s) 687, 1041, 1159
NmuCI GTSAC 1 cut(s) 620
NsiI ATGCAT 1 cut(s) 364
NspI RCATGY 1 cut(s) 766
PasI CCCWGGG 1 cut(s) 1096
PctI GAATGC 1 cut(s) 364
PfeI GAWTC 1 cut(s) 205
Ppu21I YACGTR 2 cut(s) 275, 380
Psp6I CCWGG 1 cut(s) 1095
PspGI CCWGG 1 cut(s) 1095
PspN4I GGNNCC 3 cut(s) 687, 1041, 1159
PspPI GGNCC 4 cut(s) 37, 721, 1158, 1179
PstI CTGCAG 1 cut(s) 658
PsuI RGATCY 2 cut(s) 61, 71
PvuII CAGCTG 1 cut(s) 938
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
RseI CAYNNNNRTG 1 cut(s) 639
SacII CCGCGG 1 cut(s) 97
SaqAI TTAA 6 cut(s) 201, 405, 1017, 1083, 1256, 1281
Sau96I GGNCC 4 cut(s) 37, 721, 1158, 1179
ScrFI CCNGG 7 cut(s) 305, 511, 512, 561, 695, 1097, 1116
SduI GDGCHC 1 cut(s) 673
SfaNI GCATC 5 cut(s) 38, 303, 1019, 1050, 1131
SfcI CTRYAG 1 cut(s) 654
Sfr303I CCGCGG 1 cut(s) 97
SgrBI CCGCGG 1 cut(s) 97
SinI GGWCC 1 cut(s) 1158
SmaI CCCGGG 1 cut(s) 512
SmiMI CAYNNNNRTG 1 cut(s) 639
SmlI CTYRAG 1 cut(s) 67
SmoI CTYRAG 1 cut(s) 67
SnaBI TACGTA 1 cut(s) 380
Sse9I AATT 5 cut(s) 471, 707, 995, 1018, 1079
SsiI CCGC 9 cut(s) 14, 26, 86, 94, 96, 121, 338, 341, 443
SspMI CTAG 3 cut(s) 116, 1269, 1275
StyD4I CCNGG 7 cut(s) 303, 509, 510, 559, 693, 1095, 1114
StyI CCWWGG 2 cut(s) 775, 1182
TaaI ACNGT 4 cut(s) 227, 749, 801, 1151
TaiI ACGT 2 cut(s) 277, 382
TasI AATT 5 cut(s) 471, 707, 995, 1018, 1079
TauI GCSGC 4 cut(s) 17, 29, 96, 343
TfiI GAWTC 1 cut(s) 205
Tru1I TTAA 6 cut(s) 201, 405, 1017, 1083, 1256, 1281
Tru9I TTAA 6 cut(s) 201, 405, 1017, 1083, 1256, 1281
TscAI CASTG 1 cut(s) 810
TseFI GTSAC 1 cut(s) 620
TseI GCWGC 7 cut(s) 131, 434, 656, 938, 961, 1226, 1229
Tsp45I GTSAC 1 cut(s) 620
TspDTI ATGAA 1 cut(s) 21
TspGWI ACGGA 1 cut(s) 718
TspMI CCCGGG 1 cut(s) 510
TspRI CASTG 1 cut(s) 810
VpaK11BI GGWCC 1 cut(s) 1158
XagI CCTNNNNNAGG 1 cut(s) 889
XapI RAATTY 2 cut(s) 471, 1079
XceI RCATGY 1 cut(s) 766
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XmaI CCCGGG 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 391
XspI CTAG 3 cut(s) 116, 1269, 1275
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.