Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002660

Physical Location & Seq

Genomic Coordinates Reverse (-)

16619743 .. 16624150

Gene Structure 4408 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT2G39830.4

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1587 bp

ATGGATTCTTCTTCCTCTTCCTCTTCTTCTTCTCCTTCTTCTTCCTACGGTGTTGCTCGTGTCAGCCATATCTCCAATCCTTGCATCTTCGGGGAAGTTGGGTCGTCATCTTCGTCAACGTATAGAGATAAGAAATGGAAGTTGATGAAATGGGTGAGTAAACTTTTCAAGAGTGGCTCGAATGGTGGTGGTAGTGGTGCTCACACTAATCATCATCCTCCTCAGTTTCAAGAAGACGAGAATATGGTCTTTCCTCTACCTCCTTCTTCTTTGGATGATCGGTCAAGAGGTGCACGGGACAAAGAAGAACTCGACCGTTCAATTTCACTTTCTCTAGCTGACAACACGAAGCGCCCACATGGGTATGGTTGGTCTATGGATAACAACCGAGATTTTCCAAGGCCTTTTCACGGTGGCTTGAATCCATCATCTTTCATTCCACCTTATGAGCCTTCCTATCAATATAGACGAAGACAAAGAATATGTGGCGGTTGCAATAGCGATATTGGATCGGGGAACTATCTAGGATGCATGGGCACATTCTTTCATCCTGAATGCTTCCGTTGCCATTCTTGTGGTTATGCTATCACTGAGCATGAGTTCTCTCTGTCAGGAACCAAACCCTATCATAAGCTTTGTTTCAAAGAGCTTACTCATCCCAAATGCGAAGTTTGTCACCATTTTATACCAACTAATGATGCTGGCTTGATCGAGTATCGATGCCATCCGTTTTGGAACCAAAAGTATTGCCCGTCTCACGAATATGATAAAACTGCTCGTTGTTGTAGCTGCGAACGTTTGGAGTCATGGGATGTGAGATATTACACGTTAGAGGATGGGAGAAGTTTGTGTTTAGAATGTATGGAAACCGCGATAACCGATACTGGAGAATGTCAACCGCTTTACCACGCTATAAGAGACTATTACGAAGGAATGTACATGAAACTTGATCAACAGATTCCTATGCTTCTTGTTCAAAGAGAAGCTCTCAATGATGCTATCGTAGGAGAGAAAAACGGATACCATCACATGCCTGAGACAAGAGGTTTATGCTTGTCTGAAGAACAAACAGTTACAAGTGTTCTTAGAAGACCGAGACTTGGTGCTCACCGTCTTGTTGGTATGAGAACTCAGCCTCAAAGGCTTACACGCAAATGTGAAGTCACAGCGATTCTAGTTCTTTACGGGCTCCCGCGATTACTGACCGGAGCAATTCTCGCCCATGAGCTCATGCATGGATGGCTAAGGCTTAATGGGTTTAGGAACCTTAACCCTGAGGTAGAGGAAGGAATCTGCCAAGTCCTCTCTTACATGTGGCTTGAATCTGAAGTTCTCTCAGATCCTTCAACAAGAAACTTGCCTTCAACATCATCGGTGGCCACATCATCATCATCATCCTTCTCGAACAAGAAAGGAGGAAAATCAAACGTGGAGAAGAAACTTGGAGAGTTCTTTAAACATCAGATAGCTCATGATGCGTCTCCAGCTTATGGAGGAGGTTTCAGGGCAGCAAATGCAGCGGCTTGTAAGTACGGTCTTCGTCGAACACTCGATCATATCCGCTTAACTGGAACTTTTCCTTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0022622 GO:0032182 GO:0032501 GO:0032502 GO:0043130 GO:0048364 GO:0048731 GO:0048856 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DA1-like LIM

Protein Analysis

528

Amino Acids

59.43

Weight (kDa)

8.25

Isoelectric Point (pI)

52.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LIM	PF00412	162 - 216	9.8e-08	LIM domain
DA1-like	PF12315	311 - 428	7e-53	Protein DA1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002660)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G39830 AT2G39830 AT2G39830 AT2G39830
fragaria_vesca	FvH4_7g23550 FvH4_7g23550 FvH4_7g23550 FvH4_7g23550 FvH4_7g23550 FvH4_7g23550 FvH4_7g23550
malus_domestica	MD01G1141300.v1.1 MD07G1205300.v1.1 MD07G1205400.v1.1
prunus_persica	Prupe.2G241500_v2.0.a1 Prupe.2G241500_v2.0.a1 Prupe.2G241500_v2.0.a1 Prupe.2G241500_v2.0.a1 Prupe.2G241500_v2.0.a1 Prupe.2G241500_v2.0.a1
pyrus_communis	pycom01g16160 pycom07g19190 pycom07g19200
rosa_chinensis	RchiOBHm_Chr1g0368711
rosa_laevigata	RLG00000027195
rosa_multiflora	Rmu_sc0001791.1_g000014 Rmu_sc0001791.1_g000015
rosa_roxburghii	Rroxscaffold_4G00288340
rosa_rugosa	Rorug01G0343900 Rorug01G0343900 Rorug01G0344000 Rorug01G0344000 Rorug01G0344100 Rorug01G0344100 Rorug01G0344100 Rorug01G0344100
rosa_samantha	Rh1AG351400 Rh1BG315100 Rh1CG329700 Rh1DG346600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1490
AccII	CGCG	2 cut(s)	872, 1195
AciI	CCGC	6 cut(s)	489, 870, 899, 1193, 1520, 1561
AclI	AACGTT	1 cut(s)	796
AclWI	GGATC	2 cut(s)	517, 1334
AcoI	YGGCCR	1 cut(s)	1377
AcuI	CTGAAG	2 cut(s)	1080, 1347
AfaI	GTAC	2 cut(s)	938, 1532
AfiI	CCNNNNNNNGG	3 cut(s)	410, 1100, 1490
AflIII	ACRYGT	2 cut(s)	825, 1311
AgsI	TTSAA	9 cut(s)	169, 230, 321, 421, 643, 977, 1322, 1347, 1365
AluBI	AGCT	8 cut(s)	338, 634, 649, 789, 986, 1228, 1469, 1487
AluI	AGCT	8 cut(s)	338, 634, 649, 789, 986, 1228, 1469, 1487
Alw21I	GWGCWC	4 cut(s)	202, 295, 1108, 1230
Alw26I	GTCTC	5 cut(s)	759, 912, 1031, 1090, 1485
Alw44I	GTGCAC	1 cut(s)	291
AlwI	GGATC	2 cut(s)	517, 1334
AoxI	GGCC	2 cut(s)	401, 1377
ApaLI	GTGCAC	1 cut(s)	291
ApeKI	GCWGC	3 cut(s)	789, 1508, 1517
ArsI	GACNNNNNNTTYG	2 cut(s)	1146, 1178
AspLEI	GCGC	1 cut(s)	354
AsuHPI	GGTGA	3 cut(s)	166, 668, 1100
AxyI	CCTNAGG	1 cut(s)	1275
BaeGI	GKGCMC	2 cut(s)	295, 539
BalI	TGGCCA	1 cut(s)	1379
BanII	GRGCYC	2 cut(s)	1191, 1230
BauI	CACGAG	1 cut(s)	57
BbsI	GAAGAC	4 cut(s)	240, 478, 1096, 1529
Bbv12I	GWGCWC	4 cut(s)	202, 295, 1108, 1230
BbvI	GCAGC	3 cut(s)	776, 1520, 1529
BccI	CCATC	5 cut(s)	433, 732, 830, 1032, 1233
BciVI	GTATCC	1 cut(s)	1013
BclI	TGATCA	1 cut(s)	949
BcoDI	GTCTC	5 cut(s)	759, 912, 1031, 1090, 1485
BfaI	CTAG	3 cut(s)	335, 524, 1175
BfoI	RGCGCY	1 cut(s)	355
BfuI	GTATCC	1 cut(s)	1013
BglI	GCCNNNNNGGC	1 cut(s)	1141
BisI	GCNGC	4 cut(s)	790, 1509, 1518, 1521
BlsI	GCNGC	4 cut(s)	791, 1510, 1519, 1522
BmiI	GGNNCC	4 cut(s)	616, 737, 1190, 1265
BmsI	GCATC	6 cut(s)	93, 518, 688, 710, 985, 1465
BpiI	GAAGAC	4 cut(s)	240, 478, 1096, 1529
BplI	GAGNNNNNCTC	4 cut(s)	972, 1004, 1200, 1232
BpmI	CTGGAG	2 cut(s)	906, 1467
Bpu10I	CCTNAGC	1 cut(s)	1244
Bsa29I	ATCGAT	1 cut(s)	718
BsaJI	CCNNGG	1 cut(s)	398
BsaWI	WCCGGW	1 cut(s)	1205
Bsc4I	CCNNNNNNNGG	3 cut(s)	410, 1100, 1490
Bse1I	ACTGG	2 cut(s)	889, 1573
Bse21I	CCTNAGG	1 cut(s)	1275
BseCI	ATCGAT	1 cut(s)	718
BseDI	CCNNGG	1 cut(s)	398
BseLI	CCNNNNNNNGG	3 cut(s)	410, 1100, 1490
BseMII	CTCAG	6 cut(s)	236, 582, 1026, 1145, 1266, 1350
BseNI	ACTGG	2 cut(s)	889, 1573
BseRI	GAGGAG	2 cut(s)	210, 1509
BseSI	GKGCMC	2 cut(s)	295, 539
BseXI	GCAGC	3 cut(s)	776, 1520, 1529
Bsh1236I	CGCG	2 cut(s)	872, 1195
Bsh1285I	CGRYCG	1 cut(s)	316
BshFI	GGCC	2 cut(s)	403, 1379
BshVI	ATCGAT	1 cut(s)	718
BsiEI	CGRYCG	1 cut(s)	316
BsiHKAI	GWGCWC	4 cut(s)	202, 295, 1108, 1230
BsiSI	CCGG	1 cut(s)	1206
BslFI	GGGAC	1 cut(s)	311
BslI	CCNNNNNNNGG	3 cut(s)	410, 1100, 1490
BsmAI	GTCTC	5 cut(s)	759, 912, 1031, 1090, 1485
BsmBI	CGTCTC	2 cut(s)	759, 1485
BsmFI	GGGAC	1 cut(s)	311
BsmI	GAATGC	1 cut(s)	560
BsnI	GGCC	2 cut(s)	403, 1379
Bsp1286I	GDGCHC	6 cut(s)	202, 295, 539, 1108, 1191, 1230
Bsp1407I	TGTACA	1 cut(s)	936
Bsp143I	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
BspACI	CCGC	6 cut(s)	489, 870, 899, 1193, 1520, 1561
BspANI	GGCC	2 cut(s)	403, 1379
BspCNI	CTCAG	6 cut(s)	235, 583, 1027, 1144, 1267, 1349
BspDI	ATCGAT	1 cut(s)	718
BspFNI	CGCG	2 cut(s)	872, 1195
BspHI	TCATGA	1 cut(s)	1471
BspLI	GGNNCC	4 cut(s)	616, 737, 1190, 1265
BspPI	GGATC	2 cut(s)	517, 1334
BsrGI	TGTACA	1 cut(s)	936
BsrI	ACTGG	2 cut(s)	889, 1573
BssECI	CCNNGG	1 cut(s)	398
BssMI	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
BssSI	CACGAG	1 cut(s)	57
BssT1I	CCWWGG	1 cut(s)	398
Bst2BI	CACGAG	1 cut(s)	57
Bst4CI	ACNGT	6 cut(s)	50, 317, 413, 1072, 1112, 1535
Bst6I	CTCTTC	2 cut(s)	22, 28
BstAPI	GCANNNNNTGC	1 cut(s)	1514
BstAUI	TGTACA	1 cut(s)	936
BstC8I	GCNNGC	1 cut(s)	703
BstDEI	CTNAG	8 cut(s)	222, 591, 1035, 1085, 1131, 1244, 1275, 1336
BstFNI	CGCG	2 cut(s)	872, 1195
BstH2I	RGCGCY	1 cut(s)	355
BstHHI	GCGC	1 cut(s)	354
BstKTI	GATC	6 cut(s)	280, 512, 711, 952, 1342, 1555
BstMAI	GTCTC	5 cut(s)	759, 912, 1031, 1090, 1485
BstMBI	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
BstMCI	CGRYCG	1 cut(s)	316
BstMWI	GCNNNNNNNGC	8 cut(s)	564, 1141, 1217, 1240, 1475, 1484, 1514, 1517
BstNSI	RCATGY	2 cut(s)	1033, 1315
BstSLI	GKGCMC	2 cut(s)	295, 539
BstUI	CGCG	2 cut(s)	872, 1195
BstV1I	GCAGC	3 cut(s)	776, 1520, 1529
BstV2I	GAAGAC	4 cut(s)	240, 478, 1096, 1529
BstX2I	RGATCY	1 cut(s)	1339
BstXI	CCANNNNNNTGG	1 cut(s)	575
BstYI	RGATCY	1 cut(s)	1339
Bsu15I	ATCGAT	1 cut(s)	718
Bsu36I	CCTNAGG	1 cut(s)	1275
BsuI	GTATCC	1 cut(s)	1013
BsuRI	GGCC	2 cut(s)	403, 1379
BsuTUI	ATCGAT	1 cut(s)	718
BtsIMutI	CAGTG	1 cut(s)	588
Cac8I	GCNNGC	1 cut(s)	703
CciI	TCATGA	1 cut(s)	1471
CfoI	GCGC	1 cut(s)	354
ClaI	ATCGAT	1 cut(s)	718
CseI	GACGC	1 cut(s)	1467
Csp6I	GTAC	2 cut(s)	937, 1531
CviQI	GTAC	2 cut(s)	937, 1531
DdeI	CTNAG	8 cut(s)	222, 591, 1035, 1085, 1131, 1244, 1275, 1336
DpnI	GATC	6 cut(s)	279, 511, 710, 951, 1341, 1554
DpnII	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
DraI	TTTAAA	1 cut(s)	1456
EaeI	YGGCCR	1 cut(s)	1377
Eam1104I	CTCTTC	2 cut(s)	22, 28
EarI	CTCTTC	2 cut(s)	22, 28
Ecl136II	GAGCTC	1 cut(s)	1228
Eco130I	CCWWGG	1 cut(s)	398
Eco147I	AGGCCT	1 cut(s)	403
Eco24I	GRGCYC	2 cut(s)	1191, 1230
Eco53kI	GAGCTC	1 cut(s)	1228
Eco57I	CTGAAG	2 cut(s)	1080, 1347
Eco81I	CCTNAGG	1 cut(s)	1275
EcoICRI	GAGCTC	1 cut(s)	1228
EcoT14I	CCWWGG	1 cut(s)	398
EcoT22I	ATGCAT	2 cut(s)	533, 1236
EcoT38I	GRGCYC	2 cut(s)	1191, 1230
ErhI	CCWWGG	1 cut(s)	398
Esp3I	CGTCTC	2 cut(s)	759, 1485
FaqI	GGGAC	1 cut(s)	311
FauI	CCCGC	1 cut(s)	1200
FbaI	TGATCA	1 cut(s)	949
Fnu4HI	GCNGC	4 cut(s)	790, 1509, 1518, 1521
FriOI	GRGCYC	2 cut(s)	1191, 1230
Fsp4HI	GCNGC	4 cut(s)	790, 1509, 1518, 1521
FspBI	CTAG	3 cut(s)	335, 524, 1175
GlaI	GCGC	1 cut(s)	353
GluI	GCNGC	4 cut(s)	790, 1509, 1518, 1521
GsuI	CTGGAG	2 cut(s)	906, 1467
HaeII	RGCGCY	1 cut(s)	355
HaeIII	GGCC	2 cut(s)	403, 1379
HapII	CCGG	1 cut(s)	1206
HgaI	GACGC	1 cut(s)	1467
HhaI	GCGC	1 cut(s)	354
Hin6I	GCGC	1 cut(s)	352
HinP1I	GCGC	1 cut(s)	352
HincII	GTYRAC	2 cut(s)	117, 896
HindII	GTYRAC	2 cut(s)	117, 896
HindIII	AAGCTT	1 cut(s)	632
HinfI	GANTC	7 cut(s)	5, 421, 803, 958, 1171, 1290, 1322
HpaII	CCGG	1 cut(s)	1206
HphI	GGTGA	3 cut(s)	166, 668, 1100
Hpy166II	GTNNAC	4 cut(s)	117, 161, 293, 896
Hpy188I	TCNGA	4 cut(s)	1060, 1327, 1339, 1464
Hpy188III	TCNNGA	8 cut(s)	169, 230, 285, 551, 612, 758, 1402, 1472
Hpy8I	GTNNAC	4 cut(s)	117, 161, 293, 896
Hpy99I	CGWCG	1 cut(s)	1545
HpyAV	CCTTC	8 cut(s)	45, 273, 462, 923, 1280, 1353, 1371, 1408
HpyCH4III	ACNGT	6 cut(s)	50, 317, 413, 1072, 1112, 1535
HpyCH4IV	ACGT	4 cut(s)	119, 796, 827, 1428
HpyCH4V	TGCA	6 cut(s)	84, 293, 495, 531, 1234, 1517
HpyF10VI	GCNNNNNNNGC	8 cut(s)	564, 1141, 1217, 1240, 1475, 1484, 1514, 1517
HpyF3I	CTNAG	8 cut(s)	222, 591, 1035, 1085, 1131, 1244, 1275, 1336
HpySE526I	ACGT	4 cut(s)	119, 796, 827, 1428
HspAI	GCGC	1 cut(s)	352
Ksp22I	TGATCA	1 cut(s)	949
Kzo9I	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
LmnI	GCTCC	2 cut(s)	1194, 1208
Lsp1109I	GCAGC	3 cut(s)	776, 1520, 1529
LweI	GCATC	6 cut(s)	93, 518, 688, 710, 985, 1465
MaeI	CTAG	3 cut(s)	335, 524, 1175
MaeII	ACGT	4 cut(s)	119, 796, 827, 1428
MaeIII	GTNAC	3 cut(s)	674, 1072, 1162
MalI	GATC	6 cut(s)	279, 511, 710, 951, 1341, 1554
MboI	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
MflI	RGATCY	1 cut(s)	1339
MhlI	GDGCHC	6 cut(s)	202, 295, 539, 1108, 1191, 1230
MlsI	TGGCCA	1 cut(s)	1379
MluCI	AATT	2 cut(s)	321, 1212
MluNI	TGGCCA	1 cut(s)	1379
MlyI	GAGTC	1 cut(s)	812
Mox20I	TGGCCA	1 cut(s)	1379
Mph1103I	ATGCAT	2 cut(s)	533, 1236
MscI	TGGCCA	1 cut(s)	1379
MseI	TTAA	4 cut(s)	1251, 1269, 1455, 1565
MslI	CAYNNNNRTG	4 cut(s)	363, 573, 762, 1153
Msp20I	TGGCCA	1 cut(s)	1379
MspA1I	CMGCKG	1 cut(s)	1520
MspI	CCGG	1 cut(s)	1206
Mva1269I	GAATGC	1 cut(s)	560
MvnI	CGCG	2 cut(s)	872, 1195
MwoI	GCNNNNNNNGC	8 cut(s)	564, 1141, 1217, 1240, 1475, 1484, 1514, 1517
NdeII	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
NlaIV	GGNNCC	4 cut(s)	616, 737, 1190, 1265
NmuCI	GTSAC	2 cut(s)	674, 1162
NsiI	ATGCAT	2 cut(s)	533, 1236
NspI	RCATGY	2 cut(s)	1033, 1315
PagI	TCATGA	1 cut(s)	1471
PceI	AGGCCT	1 cut(s)	403
PciI	ACATGT	1 cut(s)	1311
PcsI	WCGNNNNNNNCGW	1 cut(s)	110
PctI	GAATGC	1 cut(s)	560
PfeI	GAWTC	6 cut(s)	5, 421, 958, 1171, 1290, 1322
PflMI	CCANNNNNTGG	1 cut(s)	1490
PkrI	GCNGC	4 cut(s)	791, 1510, 1519, 1522
PleI	GAGTC	1 cut(s)	811
PpsI	GAGTC	1 cut(s)	811
PscI	ACATGT	1 cut(s)	1311
Psp124BI	GAGCTC	1 cut(s)	1230
Psp1406I	AACGTT	1 cut(s)	796
PspN4I	GGNNCC	4 cut(s)	616, 737, 1190, 1265
PsuI	RGATCY	1 cut(s)	1339
RsaI	GTAC	2 cut(s)	938, 1532
RsaNI	GTAC	2 cut(s)	937, 1531
RseI	CAYNNNNRTG	4 cut(s)	363, 573, 762, 1153
SacI	GAGCTC	1 cut(s)	1230
SaqAI	TTAA	4 cut(s)	1251, 1269, 1455, 1565
SatI	GCNGC	4 cut(s)	790, 1509, 1518, 1521
Sau3AI	GATC	6 cut(s)	277, 509, 708, 949, 1339, 1552
SchI	GAGTC	1 cut(s)	812
SduI	GDGCHC	6 cut(s)	202, 295, 539, 1108, 1191, 1230
SfaNI	GCATC	6 cut(s)	93, 518, 688, 710, 985, 1465
SmiMI	CAYNNNNRTG	4 cut(s)	363, 573, 762, 1153
Sse9I	AATT	2 cut(s)	321, 1212
SseBI	AGGCCT	1 cut(s)	403
SsiI	CCGC	6 cut(s)	489, 870, 899, 1193, 1520, 1561
SspMI	CTAG	3 cut(s)	335, 524, 1175
SstI	GAGCTC	1 cut(s)	1230
StuI	AGGCCT	1 cut(s)	403
StyI	CCWWGG	1 cut(s)	398
TaaI	ACNGT	6 cut(s)	50, 317, 413, 1072, 1112, 1535
TaiI	ACGT	4 cut(s)	122, 799, 830, 1431
TaqI	TCGA	7 cut(s)	179, 312, 711, 718, 1403, 1543, 1551
TaqII	GACCGA	2 cut(s)	270, 1108
TasI	AATT	2 cut(s)	321, 1212
TatI	WGTACW	1 cut(s)	936
TauI	GCSGC	1 cut(s)	1523
TfiI	GAWTC	6 cut(s)	5, 421, 958, 1171, 1290, 1322
Tru1I	TTAA	4 cut(s)	1251, 1269, 1455, 1565
Tru9I	TTAA	4 cut(s)	1251, 1269, 1455, 1565
TscAI	CASTG	1 cut(s)	595
TseFI	GTSAC	2 cut(s)	674, 1162
TseI	GCWGC	3 cut(s)	789, 1508, 1517
Tsp45I	GTSAC	2 cut(s)	674, 1162
TspDTI	ATGAA	4 cut(s)	161, 424, 536, 956
TspGWI	ACGGA	3 cut(s)	551, 717, 1032
TspRI	CASTG	1 cut(s)	595
Van91I	CCANNNNNTGG	1 cut(s)	1490
VneI	GTGCAC	1 cut(s)	291
XceI	RCATGY	2 cut(s)	1033, 1315
XspI	CTAG	3 cut(s)	335, 524, 1175
Zsp2I	ATGCAT	2 cut(s)	533, 1236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.