FvH4_7g23550

DA1-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0002660
Physical Location & Seq
Forward (+)
18342886 .. 18346957
4072 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g23550.t7

Sequence Viewer

Length: 1422 bp
ATGTGGTTGAAATATTTTTGGAGCGGCAACGGTCGAGGAGGAGCTGCTGGGAATCGCAATCCACAGCTTATTGGTGACGAAAACATGGTGTGGCGTGCGCCTTTTCGCTCCATGGATGAGCGGTCTAGAACACAGAAAGAGAAAGAGGAATTAGACCATGCAATCGCGCTCTCTCTGGCTGAAGATTCTAAGAGACCAAGTGGCCATGGATATAGATGGCAGGAGAAGGATAACGAGCTTAATAGGTCACTTGATAGTCTAAATCCACCTATATTCCCTCCATATGCCCCTCCAGTTCCTCAGTATTATCCGTATGGAACTAGAATCTGTGGTGGTTGTAAACAAGATATAGATTACGGTAATTATTTGGGCTGCATGGGGACATTTTTTCATCCTCAGTGCTTCTGTTGTCGCTCTTGTGGTCATCCAATTGTTGAGAATGAGTTCTCTCTATCAGGGAGGGATCCATACCATAAGTCCTGTTTCAAGGAGCTGGCCCATCCTAAATGTGAAGTTTGTCATCAATTTATTCCAACTAATCGAGCTGGTTTGATCGAGTATAGATGCCATCCATTTTGGTCACAAAAATACTGTCCATCACATGAACATGATAACACAGCTCGTTGTTGTAGTTGTGAACGCTTAGAGTCTTGGGATGCAAGATATATTTCAGTGGGAGATGGAAGGAGTCTATGCTTTGAATGTATGGAATCTGCTATCATGGACACTGGTGACTGTCAGCCATTGTATCATGCCATCAGAGATTATTATGAAGGAATGAATATGAGACTAGATCAACAAATTCCCATGCTTCTGGTCGAAAGACAAGCGCTTAATGAAGCTCTTGTTGGGGAGAAGAATGGCCTCCATCACATGCCAGAGACAAGGGGCTTATGCCTTTCTGAAGAGCAGACAGTCACCAGTATTCTCAAACGGCCGAGAATTGGAGGCCATCAACTTATAGGAATGAGAACACAACCTCAAAAGTTGACTCGAAGATGTGAAGTTACTGCCATTCTTGTTCTCTATAGTCTCCCAAGATTACTAACTGGTGCTATTCTTGCTCATGAGTTGATGCATGGCTGGTTACGTCTGAAAGGCTACCGCAATCTTAATCCTGAAGTAGAGGAAGGTATCTGTCAAGTGCTTTCATACATGTGGCTTGAAACAGAAGTAATGCCAGGATTCAAAAACATGCCATCCACGTCTACAGCAGCTTCTTCTTCCACTTCCTCATCCTCATCGAAGAAGGGAGGAAAGTCAAATGTGGAACAGAAGCTTGGTGAATTTTTCATGCATCAAATTGCCAATGATTCTTCCCCAGCATATGGAGGAGGGTTTAGAGCAGCTAATGCAGCTGTCAATAAGTTTGGTTTACGTCGTACTCTAGACCACATTCGTTTCACTGGAAGTTTCCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0022622 GO:0032182 GO:0032501 GO:0032502 GO:0043130 GO:0048364 GO:0048731 GO:0048856 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

474

Amino Acids

53.64

Weight (kDa)

7.78

Isoelectric Point (pI)

52.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LIM PF00412 133 - 187 1.3e-07 LIM domain
DA1-like PF12315 282 - 491 3e-99 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1328
AccBSI CCGCTC 2 cut(s) 24, 121
AccI GTMKAC 1 cut(s) 1208
AccII CGCG 1 cut(s) 167
AciI CCGC 3 cut(s) 24, 121, 1105
AclWI GGATC 2 cut(s) 458, 471
AcoI YGGCCR 2 cut(s) 202, 935
AcsI RAATTY 2 cut(s) 801, 1286
AcuI CTGAAG 3 cut(s) 201, 924, 1140
AfaI GTAC 1 cut(s) 1384
AfeI AGCGCT 1 cut(s) 831
AfiI CCNNNNNNNGG 2 cut(s) 944, 1328
AflIII ACRYGT 1 cut(s) 1155
AgsI TTSAA 5 cut(s) 10, 487, 701, 1166, 1189
AjiI CACGTC 1 cut(s) 1206
AjnI CCWGG 1 cut(s) 1180
AjuI GAANNNNNNNTTGG 4 cut(s) 831, 863, 1263, 1295
Alw26I GTCTC 4 cut(s) 187, 781, 875, 1037
AlwI GGATC 2 cut(s) 458, 471
Aor51HI AGCGCT 1 cut(s) 831
AoxI GGCC 5 cut(s) 202, 495, 862, 935, 949
ApeKI GCWGC 5 cut(s) 44, 372, 1214, 1346, 1355
ApoI RAATTY 2 cut(s) 801, 1286
ArsI GACNNNNNNTTYG 2 cut(s) 577, 609
Asp700I GAANNNNTTC 1 cut(s) 443
AspLEI GCGC 3 cut(s) 100, 169, 832
AspS9I GGNCC 1 cut(s) 496
AsuHPI GGTGA 4 cut(s) 86, 743, 910, 1295
BalI TGGCCA 1 cut(s) 204
BamHI GGATCC 1 cut(s) 463
BbvI GCAGC 5 cut(s) 31, 359, 1226, 1358, 1367
BccI CCATC 9 cut(s) 210, 507, 576, 604, 674, 764, 876, 960, 1207
BceAI ACGGC 1 cut(s) 950
BciT130I CCWGG 1 cut(s) 1182
BcoDI GTCTC 4 cut(s) 187, 781, 875, 1037
BfaI CTAG 4 cut(s) 126, 321, 791, 1388
BfmI CTRYAG 2 cut(s) 1027, 1209
BfoI RGCGCY 1 cut(s) 833
BisI GCNGC 6 cut(s) 25, 45, 373, 1215, 1347, 1356
BlsI GCNGC 6 cut(s) 26, 46, 374, 1216, 1348, 1357
Bme1390I CCNGG 1 cut(s) 1182
BmgBI CACGTC 1 cut(s) 1206
BmgT120I GGNCC 1 cut(s) 496
BmiI GGNNCC 1 cut(s) 465
BmrFI CCNGG 1 cut(s) 1182
BmsI GCATC 4 cut(s) 554, 646, 1065, 1306
BpmI CTGGAG 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 187
BsaJI CCNNGG 2 cut(s) 111, 205
Bsc4I CCNNNNNNNGG 2 cut(s) 944, 1328
Bse1I ACTGG 5 cut(s) 293, 733, 921, 1054, 1411
BseBI CCWGG 1 cut(s) 1182
BseDI CCNNGG 2 cut(s) 111, 205
BseGI GGATG 8 cut(s) 121, 391, 424, 499, 568, 661, 1199, 1235
BseLI CCNNNNNNNGG 2 cut(s) 944, 1328
BseMII CTCAG 2 cut(s) 314, 410
BseNI ACTGG 5 cut(s) 293, 733, 921, 1054, 1411
BseRI GAGGAG 3 cut(s) 51, 54, 1347
BseX3I CGGCCG 1 cut(s) 935
BseXI GCAGC 5 cut(s) 31, 359, 1226, 1358, 1367
BseYI CCCAGC 2 cut(s) 47, 1321
Bsh1236I CGCG 1 cut(s) 167
Bsh1285I CGRYCG 2 cut(s) 34, 938
BshFI GGCC 5 cut(s) 204, 497, 864, 937, 951
BsiEI CGRYCG 2 cut(s) 34, 938
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 2 cut(s) 944, 1328
BsmAI GTCTC 4 cut(s) 187, 781, 875, 1037
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 5 cut(s) 204, 497, 864, 937, 951
Bso31I GGTCTC 1 cut(s) 187
Bsp143I GATC 3 cut(s) 463, 552, 793
Bsp19I CCATGG 2 cut(s) 111, 205
BspACI CCGC 3 cut(s) 24, 121, 1105
BspANI GGCC 5 cut(s) 204, 497, 864, 937, 951
BspCNI CTCAG 2 cut(s) 313, 409
BspFNI CGCG 1 cut(s) 167
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 1 cut(s) 465
BspPI GGATC 2 cut(s) 458, 471
BspQI GCTCTTC 1 cut(s) 900
BspTNI GGTCTC 1 cut(s) 187
BsrBI CCGCTC 2 cut(s) 24, 121
BsrI ACTGG 5 cut(s) 293, 733, 921, 1054, 1411
BssECI CCNNGG 2 cut(s) 111, 205
BssMI GATC 3 cut(s) 463, 552, 793
BssT1I CCWWGG 2 cut(s) 111, 205
Bst2UI CCWGG 1 cut(s) 1182
Bst4CI ACNGT 5 cut(s) 32, 359, 593, 737, 916
Bst6I CTCTTC 1 cut(s) 900
BstAPI GCANNNNNTGC 1 cut(s) 1352
BstC8I GCNNGC 2 cut(s) 96, 495
BstDEI CTNAG 4 cut(s) 189, 300, 396, 643
BstDSI CCRYGG 2 cut(s) 111, 205
BstF5I GGATG 8 cut(s) 121, 391, 424, 499, 568, 661, 1199, 1235
BstFNI CGCG 1 cut(s) 167
BstH2I RGCGCY 1 cut(s) 833
BstHHI GCGC 3 cut(s) 100, 169, 832
BstKTI GATC 3 cut(s) 466, 555, 796
BstMAI GTCTC 4 cut(s) 187, 781, 875, 1037
BstMBI GATC 3 cut(s) 463, 552, 793
BstMCI CGRYCG 2 cut(s) 34, 938
BstMWI GCNNNNNNNGC 3 cut(s) 1061, 1352, 1355
BstNI CCWGG 1 cut(s) 1182
BstNSI RCATGY 3 cut(s) 877, 1159, 1198
BstSCI CCNGG 1 cut(s) 1180
BstSFI CTRYAG 2 cut(s) 1027, 1209
BstUI CGCG 1 cut(s) 167
BstV1I GCAGC 5 cut(s) 31, 359, 1226, 1358, 1367
BstX2I RGATCY 1 cut(s) 463
BstXI CCANNNNNNTGG 1 cut(s) 814
BstYI RGATCY 1 cut(s) 463
BstZI CGGCCG 1 cut(s) 935
BsuRI GGCC 5 cut(s) 204, 497, 864, 937, 951
BtgI CCRYGG 2 cut(s) 111, 205
BtrI CACGTC 1 cut(s) 1206
BtsCI GGATG 8 cut(s) 121, 391, 424, 499, 568, 661, 1199, 1235
BtsIMutI CAGTG 4 cut(s) 404, 678, 726, 1404
Cac8I GCNNGC 2 cut(s) 96, 495
CciI TCATGA 1 cut(s) 1066
CfoI GCGC 3 cut(s) 100, 169, 832
Cfr13I GGNCC 1 cut(s) 496
Csp6I GTAC 1 cut(s) 1383
CviQI GTAC 1 cut(s) 1383
DdeI CTNAG 4 cut(s) 189, 300, 396, 643
DpnI GATC 3 cut(s) 465, 554, 795
DpnII GATC 3 cut(s) 463, 552, 793
EaeI YGGCCR 2 cut(s) 202, 935
EagI CGGCCG 1 cut(s) 935
Eam1104I CTCTTC 1 cut(s) 900
EarI CTCTTC 1 cut(s) 900
EclXI CGGCCG 1 cut(s) 935
Eco130I CCWWGG 2 cut(s) 111, 205
Eco31I GGTCTC 1 cut(s) 187
Eco47III AGCGCT 1 cut(s) 831
Eco52I CGGCCG 1 cut(s) 935
Eco57I CTGAAG 3 cut(s) 201, 924, 1140
EcoRII CCWGG 1 cut(s) 1180
EcoT14I CCWWGG 2 cut(s) 111, 205
EcoT22I ATGCAT 2 cut(s) 1080, 1299
ErhI CCWWGG 2 cut(s) 111, 205
FaqI GGGAC 1 cut(s) 394
FauNDI CATATG 2 cut(s) 283, 1327
FblI GTMKAC 1 cut(s) 1208
Fnu4HI GCNGC 6 cut(s) 25, 45, 373, 1215, 1347, 1356
FokI GGATG 8 cut(s) 128, 378, 411, 486, 555, 668, 1186, 1222
Fsp4HI GCNGC 6 cut(s) 25, 45, 373, 1215, 1347, 1356
FspBI CTAG 4 cut(s) 126, 321, 791, 1388
GlaI GCGC 3 cut(s) 99, 168, 831
GluI GCNGC 6 cut(s) 25, 45, 373, 1215, 1347, 1356
GsaI CCCAGC 2 cut(s) 51, 1325
GsuI CTGGAG 1 cut(s) 276
HaeII RGCGCY 1 cut(s) 833
HaeIII GGCC 5 cut(s) 204, 497, 864, 937, 951
HhaI GCGC 3 cut(s) 100, 169, 832
Hin6I GCGC 3 cut(s) 98, 167, 830
HinP1I GCGC 3 cut(s) 98, 167, 830
HincII GTYRAC 1 cut(s) 990
HindII GTYRAC 1 cut(s) 990
HindIII AAGCTT 1 cut(s) 1277
HinfI GANTC 9 cut(s) 52, 185, 324, 647, 688, 710, 991, 1185, 1313
HphI GGTGA 4 cut(s) 86, 743, 910, 1295
Hpy166II GTNNAC 5 cut(s) 341, 638, 990, 1209, 1376
Hpy188I TCNGA 3 cut(s) 761, 904, 1095
Hpy188III TCNNGA 4 cut(s) 126, 1067, 1118, 1388
Hpy8I GTNNAC 5 cut(s) 341, 638, 990, 1209, 1376
Hpy99I CGWCG 1 cut(s) 1383
HpyAV CCTTC 5 cut(s) 220, 678, 767, 1124, 1243
HpyCH4III ACNGT 5 cut(s) 32, 359, 593, 737, 916
HpyCH4IV ACGT 3 cut(s) 1090, 1205, 1378
HpyCH4V TGCA 6 cut(s) 161, 375, 659, 1078, 1297, 1355
HpyF10VI GCNNNNNNNGC 3 cut(s) 1061, 1352, 1355
HpyF3I CTNAG 4 cut(s) 189, 300, 396, 643
HpySE526I ACGT 3 cut(s) 1090, 1205, 1378
HspAI GCGC 3 cut(s) 98, 167, 830
Kzo9I GATC 3 cut(s) 463, 552, 793
LguI GCTCTTC 1 cut(s) 900
LmnI GCTCC 4 cut(s) 21, 41, 113, 490
Lsp1109I GCAGC 5 cut(s) 31, 359, 1226, 1358, 1367
LweI GCATC 4 cut(s) 554, 646, 1065, 1306
MaeI CTAG 4 cut(s) 126, 321, 791, 1388
MaeII ACGT 3 cut(s) 1090, 1205, 1378
MaeIII GTNAC 7 cut(s) 74, 246, 579, 731, 916, 1006, 1086
MalI GATC 3 cut(s) 465, 554, 795
MbiI CCGCTC 2 cut(s) 24, 121
MboI GATC 3 cut(s) 463, 552, 793
MboII GAAGA 8 cut(s) 194, 868, 917, 1008, 1212, 1215, 1258, 1308
MfeI CAATTG 1 cut(s) 429
MflI RGATCY 1 cut(s) 463
MlsI TGGCCA 1 cut(s) 204
MluCI AATT 8 cut(s) 149, 361, 429, 524, 801, 942, 1286, 1302
MluNI TGGCCA 1 cut(s) 204
MlyI GAGTC 3 cut(s) 656, 697, 985
MmeI TCCRAC 1 cut(s) 557
Mox20I TGGCCA 1 cut(s) 204
Mph1103I ATGCAT 2 cut(s) 1080, 1299
MroXI GAANNNNTTC 1 cut(s) 443
MscI TGGCCA 1 cut(s) 204
MseI TTAA 3 cut(s) 240, 834, 1113
MslI CAYNNNNRTG 2 cut(s) 606, 1156
Msp20I TGGCCA 1 cut(s) 204
MspA1I CMGCKG 1 cut(s) 1358
MspR9I CCNGG 1 cut(s) 1182
MunI CAATTG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 1182
MvnI CGCG 1 cut(s) 167
MwoI GCNNNNNNNGC 3 cut(s) 1061, 1352, 1355
NcoI CCATGG 2 cut(s) 111, 205
NdeI CATATG 2 cut(s) 283, 1327
NdeII GATC 3 cut(s) 463, 552, 793
NlaIV GGNNCC 1 cut(s) 465
NmeAIII GCCGAG 1 cut(s) 963
NmuCI GTSAC 5 cut(s) 74, 246, 579, 731, 916
NsiI ATGCAT 2 cut(s) 1080, 1299
NspI RCATGY 3 cut(s) 877, 1159, 1198
PagI TCATGA 1 cut(s) 1066
PciI ACATGT 1 cut(s) 1155
PciSI GCTCTTC 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 443
PfeI GAWTC 6 cut(s) 52, 185, 324, 710, 1185, 1313
PflMI CCANNNNNTGG 1 cut(s) 1328
PkrI GCNGC 6 cut(s) 26, 46, 374, 1216, 1348, 1357
PleI GAGTC 3 cut(s) 655, 696, 985
PpsI GAGTC 3 cut(s) 655, 696, 985
PscI ACATGT 1 cut(s) 1155
Psp6I CCWGG 1 cut(s) 1180
PspFI CCCAGC 2 cut(s) 47, 1321
PspGI CCWGG 1 cut(s) 1180
PspN4I GGNNCC 1 cut(s) 465
PspPI GGNCC 1 cut(s) 496
PsuI RGATCY 1 cut(s) 463
PvuII CAGCTG 1 cut(s) 1358
RsaI GTAC 1 cut(s) 1384
RsaNI GTAC 1 cut(s) 1383
RseI CAYNNNNRTG 2 cut(s) 606, 1156
SapI GCTCTTC 1 cut(s) 900
SaqAI TTAA 3 cut(s) 240, 834, 1113
SatI GCNGC 6 cut(s) 25, 45, 373, 1215, 1347, 1356
Sau3AI GATC 3 cut(s) 463, 552, 793
Sau96I GGNCC 1 cut(s) 496
SchI GAGTC 3 cut(s) 656, 697, 985
ScrFI CCNGG 1 cut(s) 1182
SfaNI GCATC 4 cut(s) 554, 646, 1065, 1306
SfcI CTRYAG 2 cut(s) 1027, 1209
SmiMI CAYNNNNRTG 2 cut(s) 606, 1156
Sse9I AATT 8 cut(s) 149, 361, 429, 524, 801, 942, 1286, 1302
SsiI CCGC 3 cut(s) 24, 121, 1105
SspI AATATT 1 cut(s) 14
SspMI CTAG 4 cut(s) 126, 321, 791, 1388
StyD4I CCNGG 1 cut(s) 1180
StyI CCWWGG 2 cut(s) 111, 205
TaaI ACNGT 5 cut(s) 32, 359, 593, 737, 916
TaiI ACGT 3 cut(s) 1093, 1208, 1381
TaqI TCGA 6 cut(s) 34, 541, 555, 819, 994, 1244
TasI AATT 8 cut(s) 149, 361, 429, 524, 801, 942, 1286, 1302
TauI GCSGC 1 cut(s) 27
TfiI GAWTC 6 cut(s) 52, 185, 324, 710, 1185, 1313
Tru1I TTAA 3 cut(s) 240, 834, 1113
Tru9I TTAA 3 cut(s) 240, 834, 1113
TscAI CASTG 4 cut(s) 404, 678, 733, 1411
TseFI GTSAC 5 cut(s) 74, 246, 579, 731, 916
TseI GCWGC 5 cut(s) 44, 372, 1214, 1346, 1355
Tsp45I GTSAC 5 cut(s) 74, 246, 579, 731, 916
TspDTI ATGAA 7 cut(s) 380, 618, 786, 794, 852, 1140, 1282
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 4 cut(s) 404, 678, 733, 1411
Van91I CCANNNNNTGG 1 cut(s) 1328
XapI RAATTY 2 cut(s) 801, 1286
XbaI TCTAGA 2 cut(s) 125, 1387
XceI RCATGY 3 cut(s) 877, 1159, 1198
XmiI GTMKAC 1 cut(s) 1208
XmnI GAANNNNTTC 1 cut(s) 443
XspI CTAG 4 cut(s) 126, 321, 791, 1388
Zsp2I ATGCAT 2 cut(s) 1080, 1299
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.