Rmu_sc0001791.1_g000014

DA1-related

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001791.1
OG0002660
Physical Location & Seq
Forward (+)
80633 .. 84385
3753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001791.1_g000014.1.cds

Sequence Viewer

Length: 1440 bp
atggctccctcaggtgtcaaccagctctctcacccttgcgtttatggggagtttgtttcttcatacgcagagaggaagtcacgctttatgaaatggctgagtaaacttttcaggagcgggaacaatcgaggaggaacttctgggaatcgcaatcatcagcttattggcgacgaaaacatggtttggcgtgcgccttttagatctgtggatgagccgtctagaacccagaaagagaaagaggaattagaccatgcaattgcgctctctctggctgaagattcaaagagacacagaggatatagatggcaggacaaagataacgagctagctaggtcacttcaggatataaatccaccaatatatcctccacatgcccctccagttcctcaacataatccgtatgcagttcctcaacataatacgtatggaactagaatatgtggtggttgtcagcaagatatacgttacgggagggatccttatcataagtcctgttttaaggagctggcccatccaaaatgtgaagtttgtcatcaatttattccaacgaatcaagctggtttgatcgagtatagatgccatccattttggtcacaaaaatactgtccatcacatgaacatgataacacagctcgttgctgtagttgtgaacgcttagagtcttgggatgcaagatatatttcagtgggagatggacggagtctatgctttgaatgtatggaatcagctatcatggatactggtgattgtcagccattgtatcatgccatcagagattattatgaaggaatgaatatgaaattagatcagcaaattcccatgcttctggttgaaagacaagcacttaacgaagctattgtcggggagaagaatggcctccatcacatgcccgagacaaggggtttatgcctttctgaagagcagacagtcaccagtatactcaaaaggccaagaattggagggcatcaacttatcggaatgagaacccaacctcaaaagttgactcgaagatgtgaagttactgccattcttgttctctatagccttccaagattactaacaggtgctattcttgctcatgagttgatgcacggctggttacgtctcaaaggctaccgtaaccttaatcctgaagtagaggaaggtatctgtcaagtgctttcatacatgtggcttgaaacagaagtaatgccaggattcaaaaacatgccatccacgtctacatgtgcagcttcttcttccacttcctcatcctcatcgaaaaagggtggaaagtcgaatgttgaacagaagcttggtgaatttttcatgcatcaaattgcgcatgattcttccccagcatatggtggagggtttagagcagctaatgcagctgtcaataagtatggtttacgtcgtactctggatcacattcgactcactggaaatttcccattgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0007275 GO:0008150 GO:0009888 GO:0010087 GO:0010088 GO:0022622 GO:0032182 GO:0032501 GO:0032502 GO:0043130 GO:0048364 GO:0048731 GO:0048856 GO:0099402
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

479

Amino Acids

54.34

Weight (kDa)

8.45

Isoelectric Point (pI)

51.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1323
AccB7I CCANNNNNTGG 2 cut(s) 956, 1343
AccBSI CCGCTC 1 cut(s) 117
AccI GTMKAC 2 cut(s) 937, 1220
AciI CCGC 1 cut(s) 117
AclWI GGATC 3 cut(s) 470, 483, 1413
AcsI RAATTY 3 cut(s) 813, 1301, 1426
AcuI CTGAAG 4 cut(s) 294, 323, 936, 1152
AfaI GTAC 1 cut(s) 1399
AfiI CCNNNNNNNGG 3 cut(s) 897, 956, 1343
AflIII ACRYGT 2 cut(s) 1167, 1223
AgsI TTSAA 6 cut(s) 282, 713, 833, 1178, 1201, 1286
AjiI CACGTC 1 cut(s) 1218
AjnI CCWGG 1 cut(s) 1192
AjuI GAANNNNNNNTTGG 2 cut(s) 1278, 1310
Alw26I GTCTC 3 cut(s) 280, 887, 1109
AlwI GGATC 3 cut(s) 470, 483, 1413
Ama87I CYCGRG 1 cut(s) 890
AoxI GGCC 3 cut(s) 507, 874, 947
ApeKI GCWGC 3 cut(s) 1229, 1361, 1370
ApoI RAATTY 3 cut(s) 813, 1301, 1426
ArsI GACNNNNNNTTYG 4 cut(s) 589, 621, 843, 875
AspLEI GCGC 3 cut(s) 193, 262, 1324
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 4 cut(s) 23, 755, 922, 1310
AsuNHI GCTAGC 1 cut(s) 325
AvaI CYCGRG 1 cut(s) 890
AxyI CCTNAGG 1 cut(s) 10
BamHI GGATCC 1 cut(s) 475
BbvI GCAGC 3 cut(s) 1241, 1373, 1382
BccI CCATC 8 cut(s) 297, 519, 588, 616, 686, 776, 888, 1219
BceAI ACGGC 2 cut(s) 199, 1108
BciT130I CCWGG 1 cut(s) 1194
BciVI GTATCC 1 cut(s) 730
BcoDI GTCTC 3 cut(s) 280, 887, 1109
BfaI CTAG 4 cut(s) 219, 326, 330, 432
BfmI CTRYAG 2 cut(s) 640, 1039
BfuI GTATCC 1 cut(s) 730
BglII AGATCT 1 cut(s) 200
BisI GCNGC 3 cut(s) 1230, 1362, 1371
BlsI GCNGC 3 cut(s) 1231, 1363, 1372
Bme1390I CCNGG 1 cut(s) 1194
BmeT110I CYCGRG 1 cut(s) 890
BmgBI CACGTC 1 cut(s) 1218
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 2 cut(s) 6, 477
BmrFI CCNGG 1 cut(s) 1194
BmsI GCATC 5 cut(s) 566, 658, 973, 1077, 1321
BmtI GCTAGC 1 cut(s) 329
BpmI CTGGAG 1 cut(s) 363
BsaAI YACGTR 1 cut(s) 423
BsaBI GATNNNNATC 2 cut(s) 348, 480
Bsc4I CCNNNNNNNGG 3 cut(s) 897, 956, 1343
Bse1I ACTGG 4 cut(s) 380, 745, 933, 1426
Bse21I CCTNAGG 1 cut(s) 10
Bse8I GATNNNNATC 2 cut(s) 348, 480
BseBI CCWGG 1 cut(s) 1194
BseGI GGATG 6 cut(s) 214, 511, 580, 673, 1211, 1250
BseJI GATNNNNATC 2 cut(s) 348, 480
BseLI CCNNNNNNNGG 3 cut(s) 897, 956, 1343
BseMII CTCAG 2 cut(s) 24, 89
BseNI ACTGG 4 cut(s) 380, 745, 933, 1426
BseRI GAGGAG 1 cut(s) 144
BseXI GCAGC 3 cut(s) 1241, 1373, 1382
BseYI CCCAGC 1 cut(s) 1336
BsgI GTGCAG 1 cut(s) 1248
BshFI GGCC 3 cut(s) 509, 876, 949
BsiHKCI CYCGRG 1 cut(s) 890
BslI CCNNNNNNNGG 3 cut(s) 897, 956, 1343
BsmAI GTCTC 3 cut(s) 280, 887, 1109
BsmBI CGTCTC 1 cut(s) 1109
BsnI GGCC 3 cut(s) 509, 876, 949
BsoBI CYCGRG 1 cut(s) 890
Bsp143I GATC 5 cut(s) 200, 475, 564, 805, 1405
BspACI CCGC 1 cut(s) 117
BspANI GGCC 3 cut(s) 509, 876, 949
BspCNI CTCAG 2 cut(s) 23, 90
BspHI TCATGA 1 cut(s) 1078
BspLI GGNNCC 2 cut(s) 6, 477
BspOI GCTAGC 1 cut(s) 329
BspPI GGATC 3 cut(s) 470, 483, 1413
BspQI GCTCTTC 1 cut(s) 912
BsrBI CCGCTC 1 cut(s) 117
BsrI ACTGG 4 cut(s) 380, 745, 933, 1426
BssMI GATC 5 cut(s) 200, 475, 564, 805, 1405
BssNAI GTATAC 1 cut(s) 938
Bst1107I GTATAC 1 cut(s) 938
Bst2UI CCWGG 1 cut(s) 1194
Bst4CI ACNGT 3 cut(s) 605, 928, 1118
Bst6I CTCTTC 1 cut(s) 912
BstAPI GCANNNNNTGC 1 cut(s) 1367
BstBAI YACGTR 1 cut(s) 423
BstC8I GCNNGC 3 cut(s) 189, 327, 507
BstDEI CTNAG 3 cut(s) 10, 98, 655
BstF5I GGATG 6 cut(s) 214, 511, 580, 673, 1211, 1250
BstHHI GCGC 3 cut(s) 193, 262, 1324
BstKTI GATC 5 cut(s) 203, 478, 567, 808, 1408
BstMAI GTCTC 3 cut(s) 280, 887, 1109
BstMBI GATC 5 cut(s) 200, 475, 564, 805, 1405
BstMWI GCNNNNNNNGC 3 cut(s) 1073, 1367, 1370
BstNI CCWGG 1 cut(s) 1194
BstNSI RCATGY 5 cut(s) 374, 889, 1171, 1210, 1227
BstSCI CCNGG 1 cut(s) 1192
BstSFI CTRYAG 2 cut(s) 640, 1039
BstSNI TACGTA 1 cut(s) 423
BstV1I GCAGC 3 cut(s) 1241, 1373, 1382
BstX2I RGATCY 2 cut(s) 200, 475
BstXI CCANNNNNNTGG 1 cut(s) 826
BstYI RGATCY 2 cut(s) 200, 475
BstZ17I GTATAC 1 cut(s) 938
Bsu36I CCTNAGG 1 cut(s) 10
BsuI GTATCC 1 cut(s) 730
BsuRI GGCC 3 cut(s) 509, 876, 949
BtrI CACGTC 1 cut(s) 1218
BtsCI GGATG 6 cut(s) 214, 511, 580, 673, 1211, 1250
BtsIMutI CAGTG 2 cut(s) 690, 1419
Cac8I GCNNGC 3 cut(s) 189, 327, 507
CciI TCATGA 1 cut(s) 1078
CfoI GCGC 3 cut(s) 193, 262, 1324
Cfr13I GGNCC 1 cut(s) 508
Csp6I GTAC 1 cut(s) 1398
CviQI GTAC 1 cut(s) 1398
DdeI CTNAG 3 cut(s) 10, 98, 655
DpnI GATC 5 cut(s) 202, 477, 566, 807, 1407
DpnII GATC 5 cut(s) 200, 475, 564, 805, 1405
Eam1104I CTCTTC 1 cut(s) 912
EarI CTCTTC 1 cut(s) 912
Eco105I TACGTA 1 cut(s) 423
Eco57I CTGAAG 4 cut(s) 294, 323, 936, 1152
Eco81I CCTNAGG 1 cut(s) 10
Eco88I CYCGRG 1 cut(s) 890
EcoRII CCWGG 1 cut(s) 1192
EcoT22I ATGCAT 1 cut(s) 1314
Esp3I CGTCTC 1 cut(s) 1109
FalI AAGNNNNNCTT 2 cut(s) 68, 100
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 1342
FblI GTMKAC 2 cut(s) 937, 1220
Fnu4HI GCNGC 3 cut(s) 1230, 1362, 1371
FokI GGATG 6 cut(s) 221, 498, 567, 680, 1198, 1237
Fsp4HI GCNGC 3 cut(s) 1230, 1362, 1371
FspBI CTAG 4 cut(s) 219, 326, 330, 432
FspI TGCGCA 1 cut(s) 1323
GlaI GCGC 3 cut(s) 192, 261, 1323
GluI GCNGC 3 cut(s) 1230, 1362, 1371
GsaI CCCAGC 1 cut(s) 1340
GsuI CTGGAG 1 cut(s) 363
HaeIII GGCC 3 cut(s) 509, 876, 949
HhaI GCGC 3 cut(s) 193, 262, 1324
Hin6I GCGC 3 cut(s) 191, 260, 1322
HinP1I GCGC 3 cut(s) 191, 260, 1322
HincII GTYRAC 2 cut(s) 19, 1002
HindII GTYRAC 2 cut(s) 19, 1002
HindIII AAGCTT 1 cut(s) 1292
HphI GGTGA 4 cut(s) 23, 755, 922, 1310
Hpy166II GTNNAC 7 cut(s) 19, 104, 650, 938, 1002, 1221, 1391
Hpy188I TCNGA 3 cut(s) 773, 916, 977
Hpy188III TCNNGA 6 cut(s) 112, 219, 341, 1079, 1130, 1403
Hpy8I GTNNAC 7 cut(s) 19, 104, 650, 938, 1002, 1221, 1391
Hpy99I CGWCG 2 cut(s) 173, 1398
HpyAV CCTTC 3 cut(s) 779, 1055, 1136
HpyCH4III ACNGT 3 cut(s) 605, 928, 1118
HpyCH4IV ACGT 5 cut(s) 422, 463, 1102, 1217, 1393
HpyCH4V TGCA 7 cut(s) 254, 404, 671, 1090, 1229, 1312, 1370
HpyF10VI GCNNNNNNNGC 3 cut(s) 1073, 1367, 1370
HpyF3I CTNAG 3 cut(s) 10, 98, 655
HpySE526I ACGT 5 cut(s) 422, 463, 1102, 1217, 1393
HspAI GCGC 3 cut(s) 191, 260, 1322
Kzo9I GATC 5 cut(s) 200, 475, 564, 805, 1405
LguI GCTCTTC 1 cut(s) 912
LmnI GCTCC 3 cut(s) 10, 114, 502
Lsp1109I GCAGC 3 cut(s) 1241, 1373, 1382
LweI GCATC 5 cut(s) 566, 658, 973, 1077, 1321
MaeI CTAG 4 cut(s) 219, 326, 330, 432
MaeII ACGT 5 cut(s) 422, 463, 1102, 1217, 1393
MaeIII GTNAC 8 cut(s) 78, 333, 464, 591, 928, 1018, 1098, 1118
MalI GATC 5 cut(s) 202, 477, 566, 807, 1407
MbiI CCGCTC 1 cut(s) 117
MboI GATC 5 cut(s) 200, 475, 564, 805, 1405
MboII GAAGA 8 cut(s) 51, 287, 880, 929, 1020, 1227, 1230, 1323
MfeI CAATTG 1 cut(s) 255
MflI RGATCY 2 cut(s) 200, 475
MluCI AATT 9 cut(s) 242, 255, 536, 800, 813, 954, 1301, 1317, 1426
MlyI GAGTC 4 cut(s) 668, 709, 997, 1410
MmeI TCCRAC 1 cut(s) 569
Mph1103I ATGCAT 1 cut(s) 1314
MseI TTAA 3 cut(s) 498, 846, 1125
MslI CAYNNNNRTG 2 cut(s) 618, 1168
MspA1I CMGCKG 1 cut(s) 1373
MspR9I CCNGG 1 cut(s) 1194
MunI CAATTG 1 cut(s) 255
MvaI CCWGG 1 cut(s) 1194
MwoI GCNNNNNNNGC 3 cut(s) 1073, 1367, 1370
NdeI CATATG 1 cut(s) 1342
NdeII GATC 5 cut(s) 200, 475, 564, 805, 1405
NheI GCTAGC 1 cut(s) 325
NlaIV GGNNCC 2 cut(s) 6, 477
NmuCI GTSAC 4 cut(s) 78, 333, 591, 928
NsbI TGCGCA 1 cut(s) 1323
NsiI ATGCAT 1 cut(s) 1314
NspI RCATGY 5 cut(s) 374, 889, 1171, 1210, 1227
PagI TCATGA 1 cut(s) 1078
PciI ACATGT 2 cut(s) 1167, 1223
PciSI GCTCTTC 1 cut(s) 912
PfeI GAWTC 6 cut(s) 145, 278, 550, 722, 1197, 1328
PflFI GACNNNGTC 1 cut(s) 699
PflMI CCANNNNNTGG 2 cut(s) 956, 1343
PkrI GCNGC 3 cut(s) 1231, 1363, 1372
PleI GAGTC 4 cut(s) 667, 708, 997, 1410
PpsI GAGTC 4 cut(s) 667, 708, 997, 1410
Ppu21I YACGTR 1 cut(s) 423
PscI ACATGT 2 cut(s) 1167, 1223
Psp6I CCWGG 1 cut(s) 1192
PspFI CCCAGC 1 cut(s) 1336
PspGI CCWGG 1 cut(s) 1192
PspN4I GGNNCC 2 cut(s) 6, 477
PspPI GGNCC 1 cut(s) 508
PsuI RGATCY 2 cut(s) 200, 475
PsyI GACNNNGTC 1 cut(s) 699
PvuII CAGCTG 1 cut(s) 1373
RsaI GTAC 1 cut(s) 1399
RsaNI GTAC 1 cut(s) 1398
RseI CAYNNNNRTG 2 cut(s) 618, 1168
SapI GCTCTTC 1 cut(s) 912
SaqAI TTAA 3 cut(s) 498, 846, 1125
SatI GCNGC 3 cut(s) 1230, 1362, 1371
Sau3AI GATC 5 cut(s) 200, 475, 564, 805, 1405
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 4 cut(s) 668, 709, 997, 1410
ScrFI CCNGG 1 cut(s) 1194
SfaNI GCATC 5 cut(s) 566, 658, 973, 1077, 1321
SfcI CTRYAG 2 cut(s) 640, 1039
SmiMI CAYNNNNRTG 2 cut(s) 618, 1168
SnaBI TACGTA 1 cut(s) 423
Sse9I AATT 9 cut(s) 242, 255, 536, 800, 813, 954, 1301, 1317, 1426
SsiI CCGC 1 cut(s) 117
SspMI CTAG 4 cut(s) 219, 326, 330, 432
StyD4I CCNGG 1 cut(s) 1192
TaaI ACNGT 3 cut(s) 605, 928, 1118
TaiI ACGT 5 cut(s) 425, 466, 1105, 1220, 1396
TaqI TCGA 6 cut(s) 127, 567, 1006, 1259, 1277, 1414
TasI AATT 9 cut(s) 242, 255, 536, 800, 813, 954, 1301, 1317, 1426
TfiI GAWTC 6 cut(s) 145, 278, 550, 722, 1197, 1328
Tru1I TTAA 3 cut(s) 498, 846, 1125
Tru9I TTAA 3 cut(s) 498, 846, 1125
TscAI CASTG 2 cut(s) 690, 1426
TseFI GTSAC 4 cut(s) 78, 333, 591, 928
TseI GCWGC 3 cut(s) 1229, 1361, 1370
Tsp45I GTSAC 4 cut(s) 78, 333, 591, 928
TspDTI ATGAA 8 cut(s) 51, 104, 630, 798, 806, 812, 1152, 1297
TspGWI ACGGA 2 cut(s) 387, 712
TspRI CASTG 2 cut(s) 690, 1426
Tth111I GACNNNGTC 1 cut(s) 699
Van91I CCANNNNNTGG 2 cut(s) 956, 1343
XapI RAATTY 3 cut(s) 813, 1301, 1426
XbaI TCTAGA 1 cut(s) 218
XceI RCATGY 5 cut(s) 374, 889, 1171, 1210, 1227
XmiI GTMKAC 2 cut(s) 937, 1220
XspI CTAG 4 cut(s) 219, 326, 330, 432
Zsp2I ATGCAT 1 cut(s) 1314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.