AT2G40116

phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008969
Physical Location & Seq
Forward (+)
16751561 .. 16754599
3039 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40116.1

Sequence Viewer

Length: 1842 bp
ATGGGGAAGGAGAAGAAAACAGAGTCGTATAATAACGATAGCGGTAGTTACAATTACAGAATGTTCAAATTTTATAACCGTAAATTCAAGATCAACGAAGTCACACCCACCGACGACGTCCGTGACGCTTTTTGTCAATTCGCCGTCGGTGGCGGTGGTGGAGGAACCGATGGAGATTCAAGTGATGGTGACGGATCAACCGGTGTAATGGGTGCAGAGCAGCTTTGTTCTTTCCTCGATGATCACGGTGAGTCAACAACCGTCGCGGAGGCTCAACGGTTGATCGATGAGGTTATACGACGGAGACACCACGTCACGCGCTTCACGCGCCACGGACTTGACCTCGACGATTTCTTCAATTTCTTATTTTACGACGATCTCAATCCTCCCATTACCCCTCACGTGCATCAAGATATGACAGCTCCATTGTCACATTACTTTATATACACAGGACACAATTCGTATCTTACGGGGAATCAACTAAGCAGCGATTGTAGCGAAGTCCCTGTGATCAAAGCCTTGCAACGAGGCGTTCGAGTCATCGAGCTCGATCTTTGGCCTAACTCTACTGGGACAGATATCAATGTCCTTCACGGAAGAACGCTTACAACGCCTGTACCGCTGATGAAATGCTTGAAATCGATAAGAGATTACGCGTTTTCTAGTTCGCCTTATCCGGTTATCATCACTTTAGAGGATCATCTTACACCTGATCTTCAAGCCAAAGTTGCTGAGATGGCTACACAGATATTTGGGCAGATGTTGTATTACCCTGAATCAGATAGCTTACTGGAATTTCCTTCTCCTGCTTCACTGCTTCATCGGATAATCATCTCCACCAAACCGCCTAAAGAATATCTCGAATCGAGGAACCCGATTGTTAAACAAAAGGATAATAATGTATCTCCATCTTCAGAGGATGAAACACCTAGAACAGAGGAGATTCAGACCCTAGAAAGTATGTTGTTTGACCAAGATTTTGAAAGCAAGAGTGATAGTGATCAAGAGGATGAAGAGGCTAGTGAAGATCAGAAACCTGCATACAAACGGTTGATCACGATTCATGCTGGGAAACCAAAGGGAACGGTGAAAGAAGAGATGAAAGTTGTGGTTGATAAAGTGAGACGTTTGAGTTTAAGCGAGCAGGAACTTGACAGGACTTGTTCATCTAATAGTCAGGATGTTGTAAGGTTTACGCAGCGGAATTTGCTTCGGATATACCCTAAAGGGACAAGATTTAACTCCTCAAACTACAAACCTCTTATCGGTTGGACTCACGGAGCGCAAATGATTGCTTTCAACATGCAGGGATATGGGAAATCTCTGTGGTTGATGCACGGTATGTTTAGAGCCAATGGAGGTTGTGGATATGTCAAAAAACCTAACTTCTTGATGAAGAAAGGGTTTCACGATGAAGTCTTTGACCCTAGGAAGAAACTCCCTGTTAAAGAAACGTTAAAGGTGAAAGTGTATATGGGAGATGGATGGCGTATGGACTTCAGTCATACTCACTTTGATGCATATTCTCCTCCTGATTTCTACACTAAGATGTTCATAGTGGGTGTACCAGCGGATAACGCGAAAAAGAAGACAAAAATAATCGAAGACAATTGGTATCCAATTTGGGATGAGGAATTCAGCTTCCCATTAACCGTTCCAGAGCTTGCATTGCTTAGGATCGAAGTAAGAGAGTATGATATGTCGGAGAAGGATGATTTTGGTGGACAAACATGCTTGCCTGTTGCAGAGTTAAGACCAGGGATTCGATCTGTGCCTTTGTATGACAAAAAGGGTGAGAAAATGAAATCAGTACGGCTTCTTATGCGTTTCATCTTCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

613

Amino Acids

70.04

Weight (kDa)

5.86

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 139 - 281 1.7e-53 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 377 - 462 2.8e-29 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 485 - 586 6.5e-14 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 75
AatII GACGTC 1 cut(s) 120
Acc36I ACCTGC 1 cut(s) 1045
AccII CGCG 5 cut(s) 266, 319, 328, 656, 1580
AciI CCGC 7 cut(s) 42, 153, 266, 620, 845, 1201, 1571
AclI AACGTT 1 cut(s) 1454
AclWI GGATC 3 cut(s) 202, 705, 1685
AcsI RAATTY 5 cut(s) 68, 83, 794, 1204, 1634
AcuI CTGAAG 2 cut(s) 897, 1483
AcvI CACGTG 1 cut(s) 403
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 3 cut(s) 618, 1566, 1812
AfiI CCNNNNNNNGG 1 cut(s) 1265
AflIII ACRYGT 1 cut(s) 654
AgeI ACCGGT 1 cut(s) 200
AgsI TTSAA 8 cut(s) 67, 88, 180, 358, 637, 719, 983, 1300
AhdI GACNNNNNGTC 1 cut(s) 311
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 1 cut(s) 1756
AluBI AGCT 6 cut(s) 223, 422, 547, 786, 1641, 1663
AluI AGCT 6 cut(s) 223, 422, 547, 786, 1641, 1663
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 2 cut(s) 298, 1117
AlwI GGATC 3 cut(s) 202, 705, 1685
AoxI GGCC 1 cut(s) 557
ApeKI GCWGC 3 cut(s) 220, 486, 1198
ApoI RAATTY 5 cut(s) 68, 83, 794, 1204, 1634
ArsI GACNNNNNNTTYG 4 cut(s) 962, 994, 1746, 1778
AsiGI ACCGGT 1 cut(s) 200
AspA2I CCTAGG 1 cut(s) 1427
AspLEI GCGC 3 cut(s) 321, 330, 1285
AsuHPI GGTGA 5 cut(s) 200, 260, 1099, 1474, 1805
AsuII TTCGAA 1 cut(s) 1836
AvrII CCTAGG 1 cut(s) 1427
BaeI ACNNNNGTAYC 2 cut(s) 600, 633
BanII GRGCYC 1 cut(s) 549
BarI GAAGNNNNNNTAC 2 cut(s) 589, 621
BbrPI CACGTG 1 cut(s) 403
BbsI GAAGAC 2 cut(s) 1595, 1611
Bbv12I GWGCWC 1 cut(s) 549
BbvI GCAGC 3 cut(s) 232, 498, 1210
BccI CCATC 6 cut(s) 164, 179, 730, 916, 1475, 1479
BceAI ACGGC 2 cut(s) 128, 1829
BciT130I CCWGG 1 cut(s) 1758
BciVI GTATCC 1 cut(s) 1626
BclI TGATCA 4 cut(s) 241, 510, 1000, 1053
BcoDI GTCTC 2 cut(s) 298, 1117
BfaI CTAG 5 cut(s) 663, 930, 953, 1020, 1428
BfuAI ACCTGC 1 cut(s) 1045
BfuI GTATCC 1 cut(s) 1626
BisI GCNGC 3 cut(s) 221, 487, 1199
BlnI CCTAGG 1 cut(s) 1427
BlsI GCNGC 3 cut(s) 222, 488, 1200
Bme1390I CCNGG 1 cut(s) 1758
BmeRI GACNNNNNGTC 1 cut(s) 311
BmgBI CACGTC 1 cut(s) 313
BmiI GGNNCC 2 cut(s) 166, 872
BmrFI CCNGG 1 cut(s) 1758
BmrI ACTGGG 1 cut(s) 579
BmsI GCATC 3 cut(s) 415, 1323, 1507
BmuI ACTGGG 1 cut(s) 579
BoxI GACNNNNGTC 1 cut(s) 1500
BpiI GAAGAC 2 cut(s) 1595, 1611
Bpu10I CCTNAGC 1 cut(s) 1673
Bpu14I TTCGAA 1 cut(s) 1836
Bsa29I ATCGAT 2 cut(s) 285, 641
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 3 cut(s) 381, 830, 999
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 331, 1427, 1757
BsaWI WCCGGW 2 cut(s) 200, 676
Bsc4I CCNNNNNNNGG 1 cut(s) 1265
Bse118I RCCGGY 1 cut(s) 200
Bse1I ACTGG 2 cut(s) 574, 795
Bse3DI GCAATG 1 cut(s) 1667
Bse8I GATNNNNATC 3 cut(s) 381, 830, 999
BseBI CCWGG 1 cut(s) 1758
BseCI ATCGAT 2 cut(s) 285, 641
BseDI CCNNGG 3 cut(s) 331, 1427, 1757
BseGI GGATG 6 cut(s) 925, 1015, 1186, 1490, 1633, 1717
BseJI GATNNNNATC 3 cut(s) 381, 830, 999
BseLI CCNNNNNNNGG 1 cut(s) 1265
BseMI GCAATG 1 cut(s) 1667
BseMII CTCAG 1 cut(s) 723
BseNI ACTGG 2 cut(s) 574, 795
BseRI GAGGAG 3 cut(s) 953, 1234, 1518
BseXI GCAGC 3 cut(s) 232, 498, 1210
BseYI CCCAGC 1 cut(s) 1067
BsgI GTGCAG 1 cut(s) 234
Bsh1236I CGCG 5 cut(s) 266, 319, 328, 656, 1580
BshFI GGCC 1 cut(s) 559
BshTI ACCGGT 1 cut(s) 200
BshVI ATCGAT 2 cut(s) 285, 641
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 2 cut(s) 201, 677
BslFI GGGAC 3 cut(s) 488, 586, 1243
BslI CCNNNNNNNGG 1 cut(s) 1265
BsmAI GTCTC 2 cut(s) 298, 1117
BsmBI CGTCTC 1 cut(s) 1117
BsmFI GGGAC 3 cut(s) 488, 586, 1243
BsnI GGCC 1 cut(s) 559
Bsp119I TTCGAA 1 cut(s) 1836
Bsp1286I GDGCHC 1 cut(s) 549
BspACI CCGC 7 cut(s) 42, 153, 266, 620, 845, 1201, 1571
BspANI GGCC 1 cut(s) 559
BspCNI CTCAG 1 cut(s) 724
BspDI ATCGAT 2 cut(s) 285, 641
BspFNI CGCG 5 cut(s) 266, 319, 328, 656, 1580
BspLI GGNNCC 2 cut(s) 166, 872
BspMI ACCTGC 1 cut(s) 1045
BspPI GGATC 3 cut(s) 202, 705, 1685
BspT104I TTCGAA 1 cut(s) 1836
BsrDI GCAATG 1 cut(s) 1667
BsrFI RCCGGY 1 cut(s) 200
BsrI ACTGG 2 cut(s) 574, 795
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 3 cut(s) 331, 1427, 1757
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 1427
Bst2UI CCWGG 1 cut(s) 1758
Bst4CI ACNGT 8 cut(s) 80, 248, 262, 279, 1050, 1087, 1340, 1654
Bst6I CTCTTC 2 cut(s) 1008, 1089
BstACI GRCGYC 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 403
BstBI TTCGAA 1 cut(s) 1836
BstC8I GCNNGC 3 cut(s) 1142, 1665, 1736
BstDEI CTNAG 4 cut(s) 482, 732, 1545, 1673
BstDSI CCRYGG 1 cut(s) 331
BstF5I GGATG 6 cut(s) 925, 1015, 1186, 1490, 1633, 1717
BstFNI CGCG 5 cut(s) 266, 319, 328, 656, 1580
BstHHI GCGC 3 cut(s) 321, 330, 1285
BstMAI GTCTC 2 cut(s) 298, 1117
BstNI CCWGG 1 cut(s) 1758
BstNSI RCATGY 2 cut(s) 1306, 1734
BstPAI GACNNNNGTC 1 cut(s) 1500
BstSCI CCNGG 1 cut(s) 1756
BstUI CGCG 5 cut(s) 266, 319, 328, 656, 1580
BstV1I GCAGC 3 cut(s) 232, 498, 1210
BstV2I GAAGAC 2 cut(s) 1595, 1611
Bsu15I ATCGAT 2 cut(s) 285, 641
BsuI GTATCC 1 cut(s) 1626
BsuRI GGCC 1 cut(s) 559
BsuTUI ATCGAT 2 cut(s) 285, 641
BtgI CCRYGG 1 cut(s) 331
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 6 cut(s) 925, 1015, 1186, 1490, 1633, 1717
BtsI GCAGTG 1 cut(s) 812
BtsIMutI CAGTG 1 cut(s) 812
BveI ACCTGC 1 cut(s) 1045
Cac8I GCNNGC 3 cut(s) 1142, 1665, 1736
CfoI GCGC 3 cut(s) 321, 330, 1285
Cfr10I RCCGGY 1 cut(s) 200
ClaI ATCGAT 2 cut(s) 285, 641
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 617, 1565, 1811
CspAI ACCGGT 1 cut(s) 200
CviAII CATG 3 cut(s) 1064, 1303, 1731
CviQI GTAC 3 cut(s) 617, 1565, 1811
DdeI CTNAG 4 cut(s) 482, 732, 1545, 1673
DriI GACNNNNNGTC 1 cut(s) 311
Eam1104I CTCTTC 2 cut(s) 1008, 1089
Eam1105I GACNNNNNGTC 1 cut(s) 311
EarI CTCTTC 2 cut(s) 1008, 1089
Ecl136II GAGCTC 1 cut(s) 547
Eco130I CCWWGG 1 cut(s) 1427
Eco24I GRGCYC 1 cut(s) 549
Eco32I GATATC 1 cut(s) 580
Eco53kI GAGCTC 1 cut(s) 547
Eco57I CTGAAG 2 cut(s) 897, 1483
Eco72I CACGTG 1 cut(s) 403
EcoICRI GAGCTC 1 cut(s) 547
EcoRI GAATTC 1 cut(s) 1634
EcoRII CCWGG 1 cut(s) 1756
EcoRV GATATC 1 cut(s) 580
EcoT14I CCWWGG 1 cut(s) 1427
EcoT22I ATGCAT 1 cut(s) 1522
EcoT38I GRGCYC 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 1427
Esp3I CGTCTC 1 cut(s) 1117
FaeI CATG 3 cut(s) 1067, 1306, 1734
FaqI GGGAC 3 cut(s) 488, 586, 1243
FatI CATG 3 cut(s) 1063, 1302, 1730
FbaI TGATCA 4 cut(s) 241, 510, 1000, 1053
Fnu4HI GCNGC 3 cut(s) 221, 487, 1199
FokI GGATG 6 cut(s) 932, 1022, 1193, 1497, 1640, 1724
FriOI GRGCYC 1 cut(s) 549
Fsp4HI GCNGC 3 cut(s) 221, 487, 1199
FspBI CTAG 5 cut(s) 663, 930, 953, 1020, 1428
GlaI GCGC 3 cut(s) 320, 329, 1284
GluI GCNGC 3 cut(s) 221, 487, 1199
GsaI CCCAGC 1 cut(s) 1071
HaeIII GGCC 1 cut(s) 559
HapII CCGG 2 cut(s) 201, 677
HgaI GACGC 1 cut(s) 134
HhaI GCGC 3 cut(s) 321, 330, 1285
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 3 cut(s) 1067, 1306, 1734
Hin6I GCGC 3 cut(s) 319, 328, 1283
HinP1I GCGC 3 cut(s) 319, 328, 1283
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HpaII CCGG 2 cut(s) 201, 677
HphI GGTGA 5 cut(s) 200, 260, 1099, 1474, 1805
Hpy166II GTNNAC 4 cut(s) 255, 1194, 1565, 1724
Hpy188I TCNGA 7 cut(s) 781, 825, 916, 948, 1032, 1215, 1705
Hpy8I GTNNAC 4 cut(s) 255, 1194, 1565, 1724
Hpy99I CGWCG 7 cut(s) 116, 119, 149, 266, 303, 350, 377
HpyAV CCTTC 3 cut(s) 599, 810, 1702
HpyCH4III ACNGT 8 cut(s) 80, 248, 262, 279, 1050, 1087, 1340, 1654
HpyCH4IV ACGT 5 cut(s) 117, 312, 402, 1126, 1454
HpyCH4V TGCA 9 cut(s) 215, 406, 523, 1040, 1306, 1336, 1520, 1667, 1745
HpyF3I CTNAG 4 cut(s) 482, 732, 1545, 1673
HpySE526I ACGT 5 cut(s) 117, 312, 402, 1126, 1454
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 3 cut(s) 1067, 1306, 1734
HspAI GCGC 3 cut(s) 319, 328, 1283
Ksp22I TGATCA 4 cut(s) 241, 510, 1000, 1053
LmnI GCTCC 2 cut(s) 427, 1280
Lsp1109I GCAGC 3 cut(s) 232, 498, 1210
LweI GCATC 3 cut(s) 415, 1323, 1507
MaeI CTAG 5 cut(s) 663, 930, 953, 1020, 1428
MaeII ACGT 5 cut(s) 117, 312, 402, 1126, 1454
MaeIII GTNAC 6 cut(s) 47, 100, 122, 188, 313, 429
MfeI CAATTG 1 cut(s) 1609
MhlI GDGCHC 1 cut(s) 549
MluI ACGCGT 1 cut(s) 654
MlyI GAGTC 4 cut(s) 32, 260, 546, 1267
MmeI TCCRAC 2 cut(s) 1250, 1683
Mph1103I ATGCAT 1 cut(s) 1522
MseI TTAA 7 cut(s) 882, 1136, 1239, 1446, 1457, 1649, 1751
MslI CAYNNNNRTG 2 cut(s) 1515, 1547
MspA1I CMGCKG 3 cut(s) 622, 1201, 1571
MspI CCGG 2 cut(s) 201, 677
MspR9I CCNGG 1 cut(s) 1758
MunI CAATTG 1 cut(s) 1609
MvaI CCWGG 1 cut(s) 1758
MvnI CGCG 5 cut(s) 266, 319, 328, 656, 1580
NlaIII CATG 3 cut(s) 1067, 1306, 1734
NlaIV GGNNCC 2 cut(s) 166, 872
NmuCI GTSAC 5 cut(s) 100, 122, 188, 313, 429
NsiI ATGCAT 1 cut(s) 1522
NspI RCATGY 2 cut(s) 1306, 1734
NspV TTCGAA 1 cut(s) 1836
PcsI WCGNNNNNNNCGW 2 cut(s) 532, 872
PfeI GAWTC 7 cut(s) 176, 475, 776, 863, 943, 1060, 1762
PflFI GACNNNGTC 1 cut(s) 116
PinAI ACCGGT 1 cut(s) 200
PkrI GCNGC 3 cut(s) 222, 488, 1200
PleI GAGTC 4 cut(s) 31, 259, 545, 1267
PmaCI CACGTG 1 cut(s) 403
PmlI CACGTG 1 cut(s) 403
PpsI GAGTC 4 cut(s) 31, 259, 545, 1267
Ppu21I YACGTR 1 cut(s) 403
PshAI GACNNNNGTC 1 cut(s) 1500
PsiI TTATAA 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 549
Psp1406I AACGTT 1 cut(s) 1454
Psp6I CCWGG 1 cut(s) 1756
PspCI CACGTG 1 cut(s) 403
PspFI CCCAGC 1 cut(s) 1067
PspGI CCWGG 1 cut(s) 1756
PspN4I GGNNCC 2 cut(s) 166, 872
PsyI GACNNNGTC 1 cut(s) 116
RsaI GTAC 3 cut(s) 618, 1566, 1812
RsaNI GTAC 3 cut(s) 617, 1565, 1811
RseI CAYNNNNRTG 2 cut(s) 1515, 1547
SacI GAGCTC 1 cut(s) 549
SaqAI TTAA 7 cut(s) 882, 1136, 1239, 1446, 1457, 1649, 1751
SatI GCNGC 3 cut(s) 221, 487, 1199
SchI GAGTC 4 cut(s) 32, 260, 546, 1267
ScrFI CCNGG 1 cut(s) 1758
SduI GDGCHC 1 cut(s) 549
SfaNI GCATC 3 cut(s) 415, 1323, 1507
SfuI TTCGAA 1 cut(s) 1836
SmiMI CAYNNNNRTG 2 cut(s) 1515, 1547
SsiI CCGC 7 cut(s) 42, 153, 266, 620, 845, 1201, 1571
SspMI CTAG 5 cut(s) 663, 930, 953, 1020, 1428
SstI GAGCTC 1 cut(s) 549
StyD4I CCNGG 1 cut(s) 1756
StyI CCWWGG 1 cut(s) 1427
TaaI ACNGT 8 cut(s) 80, 248, 262, 279, 1050, 1087, 1340, 1654
TaiI ACGT 5 cut(s) 120, 315, 405, 1129, 1457
TfiI GAWTC 7 cut(s) 176, 475, 776, 863, 943, 1060, 1762
Tru1I TTAA 7 cut(s) 882, 1136, 1239, 1446, 1457, 1649, 1751
Tru9I TTAA 7 cut(s) 882, 1136, 1239, 1446, 1457, 1649, 1751
TscAI CASTG 1 cut(s) 819
TseFI GTSAC 5 cut(s) 100, 122, 188, 313, 429
TseI GCWGC 3 cut(s) 220, 486, 1198
Tsp45I GTSAC 5 cut(s) 100, 122, 188, 313, 429
TspGWI ACGGA 6 cut(s) 110, 207, 316, 348, 609, 1293
TspRI CASTG 1 cut(s) 819
Tth111I GACNNNGTC 1 cut(s) 116
XapI RAATTY 5 cut(s) 68, 83, 794, 1204, 1634
XceI RCATGY 2 cut(s) 1306, 1734
XmaJI CCTAGG 1 cut(s) 1427
XspI CTAG 5 cut(s) 663, 930, 953, 1020, 1428
ZraI GACGTC 1 cut(s) 118
Zsp2I ATGCAT 1 cut(s) 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.