pycom01g17050

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0008969
Physical Location & Seq
Forward (+)
16442685 .. 16445654
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g17050.1

Sequence Viewer

Length: 1737 bp
ATGGGGGACAGCAATGCACCGCACCACAATTACAAGATGTTCAAGTGCTTCAACCGGAAGTTCAAGGCCAGCGAGGCCGGCCCGCCGCCCGACGTCAAGGCGGTGTTCGCCGAGTACGCGCAGGGTGGGGACCGGATGTCCGTCGACCAGTTCCGGAGCTTTCTGGTCGAGCACCAGGGCGAGTCGGCCCTCACTATCTCCGACGCCCATACTATCCTCCACCAGTTCACGACCCGCCACCCGGGCGAGGCCGCGCAGCACAAGAAGCACGCGGGGGGACTCTCTCTCGAGGATTTCTTTCATTTCTTGTTCCTTGAAGAGTTCAACGCCCCAATCAGAAGTCAGGTACACCATGATATGAATGCTCCAGTATCACATTACTTCATATATACAGGGCATAATTCCTACCTTACTGGGAATCAACTGAGCAGTGATTGCAGCGATGTCCCAATCATCAAGGCATTGCAAAAGGGTGTTAAAGTAATTGAACTTGATCTGTGGCCAAACTCTGCAAAAGATGATGTTCAAGTTTATCATGGAAGGACCTTGACCGCTCCTGTGACGCTAATCAAATGCTTGAAGTCGATAAAAGAACATGCTTTTGATAAATCACCATACCCCGTTGTTATAACTTTTGAAGATCACCTTACTCCCAAGCTTCAGGCTAAAGTTGCAGAGTTGGTTATCAAAACATTTGGAGACACGCTGTATTATCCGGAGGCAGAAGACCAAATGGTAGAATTCCCATCACCCGAATCCTTAAAAAAGCGAATTATTATCTCAACAAAACCGCCAAAAGAAAGCAGCGGATCAAAGAGTTCAAAGGGCAAGGGTAGTGGAAGGTCTTCATCTGAAGAGAGTGGCAGTGATCAAGATGGCGACGCTATCAACAGTGCCAATGCAGCTGAAGCCAAATCATACCAACAATCAGCGCCCGAGTACAAGCGTCTCATAACAATCCGCGCTGGAAAACCATCTGGTGAATTAAAGGATGCTCTAGCAGTCGGTGATAAGGTTAGACGTCTTAGTTTGAGTGAAGGAAAACTTGAAAAGGTGGCTGAAGATCATGGAACTGATGTCGTAAGGTTCACACAGAAGAATGTTCTAAGGGTGTACCCGAAAGGAACACGATTCAACTCCTCAAATTACGAACCACATGTTGGCTGGATGCATGGAGCTCAAATGGTTGCATTTAATATGCAGGGAAAGGACAAGTACAATTGGCTGATGCATGGGATGTTTAAAGCCAACGGAGGGTGTGGTTACTTAAAAAAGCCGGACTTTTTGATGTGCAAGGGTCCGAAAGGTGAGGTATTCGATCCTAAAAACGCCTTGAAAGTGAAAAAGACATTGAAGGTGAAAATATACATGGGAAATGGATGGCACTTGGATTTTAGCCGAACGCACTTTGATTCCTTCTCCCCACCCGACTTCTACACAAAGGTCTATATTGTTGGAGTGCCAGCAGATTGTGGTAAATTCAAAACAAAGATAATTGAGGATGATTGGATACCTGTATGGGACGAAGAGTTTACATTCCCTATGACTGTTCCGGAGCTTGCCATACTCCGGTTAGAAGTCCGGGAGTATGACAGGTCAGAGAAAGATGATTTTGGCGGGCAGACGTGTCTGCCCGTTTCCGAGCTGAAGCCCGGAATCAGGGCAGTCCCCCTGTATGATAAAAAGGGGGAGAAATACAAATCTGTAAGGCTTCTAATAAGGTTTCATTTTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

579

Amino Acids

65.06

Weight (kDa)

8.5

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_like PF09279 31 - 107 3.4e-06 Phosphoinositide-specific phospholipase C, efhand-like
PI-PLC-X PF00388 120 - 263 8.5e-53 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 342 - 428 3.1e-28 Phosphatidylinositol-specific phospholipase C, Y domain
C2 PF00168 450 - 552 6e-15 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 629
AatII GACGTC 2 cut(s) 96, 1024
AccB7I CCANNNNNTGG 1 cut(s) 1160
AccBSI CCGCTC 1 cut(s) 554
AccI GTMKAC 1 cut(s) 144
AccII CGCG 4 cut(s) 119, 254, 272, 963
AccIII TCCGGA 3 cut(s) 153, 715, 1552
AclWI GGATC 2 cut(s) 817, 1313
AcoI YGGCCR 1 cut(s) 500
AcsI RAATTY 2 cut(s) 740, 1478
AcuI CTGAAG 5 cut(s) 644, 873, 927, 1080, 1667
AcyI GRCGYC 3 cut(s) 93, 204, 1021
AfaI GTAC 5 cut(s) 116, 348, 941, 1115, 1217
AfiI CCNNNNNNNGG 6 cut(s) 241, 247, 1160, 1254, 1569, 1659
AflIII ACRYGT 2 cut(s) 1156, 1625
AhdI GACNNNNNGTC 1 cut(s) 136
AjiI CACGTC 1 cut(s) 1626
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 2 cut(s) 1143, 1175
AluBI AGCT 6 cut(s) 159, 658, 905, 1178, 1558, 1645
AluI AGCT 6 cut(s) 159, 658, 905, 1178, 1558, 1645
Alw21I GWGCWC 2 cut(s) 174, 1180
Alw26I GTCTC 2 cut(s) 693, 953
AlwI GGATC 2 cut(s) 817, 1313
Ama87I CYCGRG 3 cut(s) 241, 287, 935
Aor13HI TCCGGA 3 cut(s) 153, 715, 1552
AoxI GGCC 6 cut(s) 66, 75, 79, 186, 249, 500
ApeKI GCWGC 4 cut(s) 256, 438, 804, 902
ApoI RAATTY 2 cut(s) 740, 1478
Asp700I GAANNNNTTC 1 cut(s) 844
AspLEI GCGC 4 cut(s) 121, 256, 934, 965
AspS9I GGNCC 5 cut(s) 80, 130, 187, 543, 1298
AsuC2I CCSGG 4 cut(s) 242, 243, 1583, 1653
AsuHPI GGTGA 7 cut(s) 603, 635, 741, 992, 1019, 1319, 1369
AvaI CYCGRG 3 cut(s) 241, 287, 935
AvaII GGWCC 3 cut(s) 130, 543, 1298
BalI TGGCCA 1 cut(s) 502
BanII GRGCYC 1 cut(s) 1180
BbsI GAAGAC 2 cut(s) 732, 837
Bbv12I GWGCWC 2 cut(s) 174, 1180
BbvI GCAGC 4 cut(s) 268, 450, 816, 914
BccI CCATC 4 cut(s) 754, 869, 982, 1374
BciT130I CCWGG 1 cut(s) 176
BciVI GTATCC 1 cut(s) 1503
BclI TGATCA 1 cut(s) 868
BcnI CCSGG 4 cut(s) 242, 243, 1583, 1653
BcoDI GTCTC 2 cut(s) 693, 953
BfaI CTAG 1 cut(s) 998
BfoI RGCGCY 1 cut(s) 935
BfuI GTATCC 1 cut(s) 1503
BglI GCCNNNNNGGC 2 cut(s) 74, 243
BisI GCNGC 6 cut(s) 86, 252, 257, 439, 805, 903
BlsI GCNGC 6 cut(s) 87, 253, 258, 440, 806, 904
Bme1390I CCNGG 5 cut(s) 176, 242, 243, 1583, 1653
Bme18I GGWCC 3 cut(s) 130, 543, 1298
BmeRI GACNNNNNGTC 1 cut(s) 136
BmeT110I CYCGRG 3 cut(s) 241, 287, 935
BmgBI CACGTC 1 cut(s) 1626
BmgT120I GGNCC 5 cut(s) 80, 130, 187, 543, 1298
BmiI GGNNCC 2 cut(s) 131, 1299
BmrFI CCNGG 5 cut(s) 176, 242, 243, 1583, 1653
BmrI ACTGGG 1 cut(s) 423
BmsI GCATC 3 cut(s) 982, 1158, 1218
BmuI ACTGGG 1 cut(s) 423
BpiI GAAGAC 2 cut(s) 732, 837
BpmI CTGGAG 1 cut(s) 351
BpuMI CCSGG 4 cut(s) 242, 243, 1583, 1653
BsaHI GRCGYC 3 cut(s) 93, 204, 1021
BsaJI CCNNGG 2 cut(s) 175, 241
BsaWI WCCGGW 6 cut(s) 54, 132, 153, 715, 1552, 1569
Bsc4I CCNNNNNNNGG 6 cut(s) 241, 247, 1160, 1254, 1569, 1659
Bse118I RCCGGY 1 cut(s) 77
Bse1I ACTGG 4 cut(s) 148, 223, 368, 418
Bse3DI GCAATG 2 cut(s) 19, 461
BseAI TCCGGA 3 cut(s) 153, 715, 1552
BseBI CCWGG 1 cut(s) 176
BseDI CCNNGG 2 cut(s) 175, 241
BseGI GGATG 6 cut(s) 141, 997, 1173, 1242, 1385, 1507
BseLI CCNNNNNNNGG 6 cut(s) 241, 247, 1160, 1254, 1569, 1659
BseMI GCAATG 2 cut(s) 19, 461
BseMII CTCAG 1 cut(s) 416
BseNI ACTGG 4 cut(s) 148, 223, 368, 418
BseRI GAGGAG 1 cut(s) 1129
BseXI GCAGC 4 cut(s) 268, 450, 816, 914
Bsh1236I CGCG 4 cut(s) 119, 254, 272, 963
BshFI GGCC 6 cut(s) 68, 77, 81, 188, 251, 502
BsiHKAI GWGCWC 2 cut(s) 174, 1180
BsiHKCI CYCGRG 3 cut(s) 241, 287, 935
BslFI GGGAC 6 cut(s) 20, 143, 291, 431, 1535, 1652
BslI CCNNNNNNNGG 6 cut(s) 241, 247, 1160, 1254, 1569, 1659
BsmAI GTCTC 2 cut(s) 693, 953
BsmBI CGTCTC 1 cut(s) 953
BsmFI GGGAC 6 cut(s) 20, 143, 291, 431, 1535, 1652
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 6 cut(s) 68, 77, 81, 188, 251, 502
BsoBI CYCGRG 3 cut(s) 241, 287, 935
Bsp1286I GDGCHC 2 cut(s) 174, 1180
Bsp13I TCCGGA 3 cut(s) 153, 715, 1552
Bsp143I GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
BspANI GGCC 6 cut(s) 68, 77, 81, 188, 251, 502
BspCNI CTCAG 1 cut(s) 417
BspEI TCCGGA 3 cut(s) 153, 715, 1552
BspFNI CGCG 4 cut(s) 119, 254, 272, 963
BspLI GGNNCC 2 cut(s) 131, 1299
BspPI GGATC 2 cut(s) 817, 1313
BsrBI CCGCTC 1 cut(s) 554
BsrDI GCAATG 2 cut(s) 19, 461
BsrFI RCCGGY 1 cut(s) 77
BsrI ACTGG 4 cut(s) 148, 223, 368, 418
BssAI RCCGGY 1 cut(s) 77
BssECI CCNNGG 2 cut(s) 175, 241
BssMI GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
BssNI GRCGYC 3 cut(s) 93, 204, 1021
Bst2UI CCWGG 1 cut(s) 176
Bst4CI ACNGT 2 cut(s) 893, 1549
Bst6I CTCTTC 3 cut(s) 312, 849, 1521
BstACI GRCGYC 3 cut(s) 93, 204, 1021
BstAPI GCANNNNNTGC 1 cut(s) 435
BstC8I GCNNGC 7 cut(s) 70, 79, 83, 270, 1464, 1560, 1619
BstDEI CTNAG 3 cut(s) 425, 1025, 1106
BstF5I GGATG 6 cut(s) 141, 997, 1173, 1242, 1385, 1507
BstFNI CGCG 4 cut(s) 119, 254, 272, 963
BstH2I RGCGCY 1 cut(s) 935
BstHHI GCGC 4 cut(s) 121, 256, 934, 965
BstKTI GATC 6 cut(s) 496, 643, 812, 871, 1066, 1321
BstMAI GTCTC 2 cut(s) 693, 953
BstMBI GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
BstNI CCWGG 1 cut(s) 176
BstNSI RCATGY 2 cut(s) 599, 1160
BstSCI CCNGG 5 cut(s) 174, 240, 241, 1581, 1651
BstUI CGCG 4 cut(s) 119, 254, 272, 963
BstV1I GCAGC 4 cut(s) 268, 450, 816, 914
BstV2I GAAGAC 2 cut(s) 732, 837
BsuI GTATCC 1 cut(s) 1503
BsuRI GGCC 6 cut(s) 68, 77, 81, 188, 251, 502
BtgZI GCGATG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 1626
BtsCI GGATG 6 cut(s) 141, 997, 1173, 1242, 1385, 1507
BtsI GCAGTG 2 cut(s) 436, 871
BtsIMutI CAGTG 3 cut(s) 436, 871, 898
Cac8I GCNNGC 7 cut(s) 70, 79, 83, 270, 1464, 1560, 1619
CfoI GCGC 4 cut(s) 121, 256, 934, 965
Cfr10I RCCGGY 1 cut(s) 77
Cfr13I GGNCC 5 cut(s) 80, 130, 187, 543, 1298
Cfr9I CCCGGG 1 cut(s) 241
CseI GACGC 4 cut(s) 212, 571, 890, 935
Csp6I GTAC 5 cut(s) 115, 347, 940, 1114, 1216
CspCI CAANNNNNGTGG 2 cut(s) 817, 852
CviAII CATG 8 cut(s) 353, 536, 596, 1067, 1157, 1172, 1232, 1369
CviQI GTAC 5 cut(s) 115, 347, 940, 1114, 1216
DdeI CTNAG 3 cut(s) 425, 1025, 1106
DpnI GATC 6 cut(s) 495, 642, 811, 870, 1065, 1320
DpnII GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
DraI TTTAAA 1 cut(s) 1243
DriI GACNNNNNGTC 1 cut(s) 136
EaeI YGGCCR 1 cut(s) 500
Eam1104I CTCTTC 3 cut(s) 312, 849, 1521
Eam1105I GACNNNNNGTC 1 cut(s) 136
EarI CTCTTC 3 cut(s) 312, 849, 1521
Ecl136II GAGCTC 1 cut(s) 1178
Eco24I GRGCYC 1 cut(s) 1180
Eco47I GGWCC 3 cut(s) 130, 543, 1298
Eco53kI GAGCTC 1 cut(s) 1178
Eco57I CTGAAG 5 cut(s) 644, 873, 927, 1080, 1667
Eco88I CYCGRG 3 cut(s) 241, 287, 935
EcoICRI GAGCTC 1 cut(s) 1178
EcoO109I RGGNCCY 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 740
EcoRII CCWGG 1 cut(s) 174
EcoT22I ATGCAT 2 cut(s) 1173, 1233
EcoT38I GRGCYC 1 cut(s) 1180
Esp3I CGTCTC 1 cut(s) 953
FaeI CATG 8 cut(s) 356, 539, 599, 1070, 1160, 1175, 1235, 1372
FalI AAGNNNNNCTT 6 cut(s) 630, 662, 1029, 1061, 1265, 1297
FaqI GGGAC 6 cut(s) 20, 143, 291, 431, 1535, 1652
FatI CATG 8 cut(s) 352, 535, 595, 1066, 1156, 1171, 1231, 1368
FauI CCCGC 4 cut(s) 90, 242, 265, 1610
FbaI TGATCA 1 cut(s) 868
FblI GTMKAC 1 cut(s) 144
Fnu4HI GCNGC 6 cut(s) 86, 252, 257, 439, 805, 903
FokI GGATG 6 cut(s) 148, 1004, 1180, 1249, 1392, 1514
FriOI GRGCYC 1 cut(s) 1180
FseI GGCCGGCC 1 cut(s) 81
Fsp4HI GCNGC 6 cut(s) 86, 252, 257, 439, 805, 903
FspBI CTAG 1 cut(s) 998
GlaI GCGC 4 cut(s) 120, 255, 933, 964
GluI GCNGC 6 cut(s) 86, 252, 257, 439, 805, 903
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 935
HaeIII GGCC 6 cut(s) 68, 77, 81, 188, 251, 502
HgaI GACGC 4 cut(s) 212, 571, 890, 935
HhaI GCGC 4 cut(s) 121, 256, 934, 965
Hin1I GRCGYC 3 cut(s) 93, 204, 1021
Hin1II CATG 8 cut(s) 356, 539, 599, 1070, 1160, 1175, 1235, 1372
Hin6I GCGC 4 cut(s) 119, 254, 932, 963
HinP1I GCGC 4 cut(s) 119, 254, 932, 963
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 656
HinfI GANTC 7 cut(s) 182, 279, 418, 755, 1131, 1412, 1656
HphI GGTGA 7 cut(s) 603, 635, 741, 992, 1019, 1319, 1369
Hpy166II GTNNAC 6 cut(s) 145, 228, 349, 1089, 1114, 1533
Hpy188I TCNGA 6 cut(s) 202, 338, 853, 1302, 1600, 1642
Hpy188III TCNNGA 6 cut(s) 154, 229, 287, 716, 872, 1553
Hpy8I GTNNAC 6 cut(s) 145, 228, 349, 1089, 1114, 1533
Hpy99I CGWCG 4 cut(s) 95, 146, 206, 884
HpyAV CCTTC 5 cut(s) 534, 834, 1031, 1348, 1426
HpyCH4III ACNGT 2 cut(s) 893, 1549
HpyCH4IV ACGT 3 cut(s) 93, 1021, 1625
HpyF3I CTNAG 3 cut(s) 425, 1025, 1106
HpySE526I ACGT 3 cut(s) 93, 1021, 1625
Hsp92I GRCGYC 3 cut(s) 93, 204, 1021
Hsp92II CATG 8 cut(s) 356, 539, 599, 1070, 1160, 1175, 1235, 1372
HspAI GCGC 4 cut(s) 119, 254, 932, 963
Kpn2I TCCGGA 3 cut(s) 153, 715, 1552
KroI GCCGGC 1 cut(s) 77
KroNI GCCGGC 1 cut(s) 79
Ksp22I TGATCA 1 cut(s) 868
Kzo9I GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
LmnI GCTCC 5 cut(s) 156, 370, 559, 1175, 1555
Lsp1109I GCAGC 4 cut(s) 268, 450, 816, 914
LweI GCATC 3 cut(s) 982, 1158, 1218
MaeI CTAG 1 cut(s) 998
MaeII ACGT 3 cut(s) 93, 1021, 1625
MaeIII GTNAC 2 cut(s) 559, 1262
MalI GATC 6 cut(s) 495, 642, 811, 870, 1065, 1320
MbiI CCGCTC 1 cut(s) 554
MboI GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
MboII GAAGA 8 cut(s) 329, 650, 737, 837, 866, 1073, 1108, 1538
MfeI CAATTG 1 cut(s) 1219
MhlI GDGCHC 2 cut(s) 174, 1180
MlsI TGGCCA 1 cut(s) 502
MluNI TGGCCA 1 cut(s) 502
MlyI GAGTC 2 cut(s) 191, 273
MmeI TCCRAC 2 cut(s) 225, 1435
Mox20I TGGCCA 1 cut(s) 502
Mph1103I ATGCAT 2 cut(s) 1173, 1233
MroI TCCGGA 3 cut(s) 153, 715, 1552
MroNI GCCGGC 1 cut(s) 77
MroXI GAANNNNTTC 1 cut(s) 844
MscI TGGCCA 1 cut(s) 502
MseI TTAA 6 cut(s) 477, 761, 986, 1194, 1242, 1268
Msp20I TGGCCA 1 cut(s) 502
MspA1I CMGCKG 2 cut(s) 807, 905
MspR9I CCNGG 5 cut(s) 176, 242, 243, 1583, 1653
MunI CAATTG 1 cut(s) 1219
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 1 cut(s) 176
MvnI CGCG 4 cut(s) 119, 254, 272, 963
NaeI GCCGGC 1 cut(s) 79
NciI CCSGG 4 cut(s) 242, 243, 1583, 1653
NdeII GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
NgoMIV GCCGGC 1 cut(s) 77
NlaIII CATG 8 cut(s) 356, 539, 599, 1070, 1160, 1175, 1235, 1372
NlaIV GGNNCC 2 cut(s) 131, 1299
NmeAIII GCCGAG 1 cut(s) 136
NmuCI GTSAC 1 cut(s) 559
NsiI ATGCAT 2 cut(s) 1173, 1233
NspI RCATGY 2 cut(s) 599, 1160
PaeR7I CTCGAG 1 cut(s) 287
PciI ACATGT 1 cut(s) 1156
PctI GAATGC 1 cut(s) 367
PdiI GCCGGC 1 cut(s) 79
PdmI GAANNNNTTC 1 cut(s) 844
PfeI GAWTC 5 cut(s) 418, 755, 1131, 1412, 1656
PflMI CCANNNNNTGG 1 cut(s) 1160
PfoI TCCNGGA 1 cut(s) 1581
PkrI GCNGC 6 cut(s) 87, 253, 258, 440, 806, 904
PleI GAGTC 2 cut(s) 190, 273
PpsI GAGTC 2 cut(s) 190, 273
PpuMI RGGWCCY 1 cut(s) 543
PscI ACATGT 1 cut(s) 1156
PsiI TTATAA 1 cut(s) 629
Psp124BI GAGCTC 1 cut(s) 1180
Psp5II RGGWCCY 1 cut(s) 543
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PspN4I GGNNCC 2 cut(s) 131, 1299
PspPI GGNCC 5 cut(s) 80, 130, 187, 543, 1298
PspPPI RGGWCCY 1 cut(s) 543
PvuII CAGCTG 1 cut(s) 905
RigI GGCCGGCC 1 cut(s) 81
RsaI GTAC 5 cut(s) 116, 348, 941, 1115, 1217
RsaNI GTAC 5 cut(s) 115, 347, 940, 1114, 1216
SacI GAGCTC 1 cut(s) 1180
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 6 cut(s) 477, 761, 986, 1194, 1242, 1268
SatI GCNGC 6 cut(s) 86, 252, 257, 439, 805, 903
Sau3AI GATC 6 cut(s) 493, 640, 809, 868, 1063, 1318
Sau96I GGNCC 5 cut(s) 80, 130, 187, 543, 1298
SchI GAGTC 2 cut(s) 191, 273
ScrFI CCNGG 5 cut(s) 176, 242, 243, 1583, 1653
SduI GDGCHC 2 cut(s) 174, 1180
SfaNI GCATC 3 cut(s) 982, 1158, 1218
SfiI GGCCNNNNNGGCC 1 cut(s) 74
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 3 cut(s) 130, 543, 1298
SlaI CTCGAG 1 cut(s) 287
SmaI CCCGGG 1 cut(s) 243
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
SspMI CTAG 1 cut(s) 998
SstI GAGCTC 1 cut(s) 1180
StyD4I CCNGG 5 cut(s) 174, 240, 241, 1581, 1651
TaaI ACNGT 2 cut(s) 893, 1549
TaiI ACGT 3 cut(s) 96, 1024, 1628
TaqI TCGA 5 cut(s) 144, 168, 288, 584, 1317
TatI WGTACW 2 cut(s) 939, 1215
TauI GCSGC 2 cut(s) 88, 254
TfiI GAWTC 5 cut(s) 418, 755, 1131, 1412, 1656
Tru1I TTAA 6 cut(s) 477, 761, 986, 1194, 1242, 1268
Tru9I TTAA 6 cut(s) 477, 761, 986, 1194, 1242, 1268
TscAI CASTG 3 cut(s) 436, 871, 898
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 4 cut(s) 256, 438, 804, 902
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 5 cut(s) 290, 373, 374, 837, 1715
TspGWI ACGGA 2 cut(s) 130, 1266
TspMI CCCGGG 1 cut(s) 241
TspRI CASTG 3 cut(s) 436, 871, 898
Van91I CCANNNNNTGG 1 cut(s) 1160
VpaK11BI GGWCC 3 cut(s) 130, 543, 1298
XapI RAATTY 2 cut(s) 740, 1478
XceI RCATGY 2 cut(s) 599, 1160
XcmI CCANNNNNNNNNTGG 1 cut(s) 1161
XhoI CTCGAG 1 cut(s) 287
XmaI CCCGGG 1 cut(s) 241
XmiI GTMKAC 1 cut(s) 144
XmnI GAANNNNTTC 1 cut(s) 844
XspI CTAG 1 cut(s) 998
ZraI GACGTC 2 cut(s) 94, 1022
Zsp2I ATGCAT 2 cut(s) 1173, 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.