Rw1G031980

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008969
Physical Location & Seq
Forward (+)
60182129 .. 60185359
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G031980.1

Sequence Viewer

Length: 1494 bp
ATGTCCCATTCTCATGGATCATCATCGGAATATCCAAAATCCAAAACCAAAGAGAGTAAGACTTTCGGCTCAGCATCGGAGAAAAAGATCAAGAAAATGGGCGACGGAAATAACACCAACAACTACAAGATGTTCAAGTTCTTCAACCGCAAGTTCAAGATCAGCGAGTTGAAGCCACCTCCCGACGTCAAGGACATCTTCTCCAAGTTCGCCGCCGGCGGGGAACTCATATCCCTCGATCAGTTCCATACGTTCTTGATCGAACACCAGGCCGAGAAATCAGTTTCTTTGGCCGATGCCAAGCGCATCCTACATGAGTTCTCGCTCCGACGACAGCATCTTACGGACCCGAAACACAAGAAACAGTCCAAAGAGAACAAAACTGCAGCGCCGGCCCACCAAACACATGAGCACGGCCTCACTTTGGACGAATTCTTCAATTTCCTATTCGATGAAGAATTCAACGGCCCCATCAAACGAGAGGTACACCATGATATGACTGCTCCGTTGTCGCATTATTTCATATATACAGGGCACAATTCGTACCTTACTGGGAATCAACTCAGCAGTGACTGTAGTGATGTGCCAATCATTAAGGCTTTGGAGAGAGGGGTCAAAGTAATTGAGCTTGATTTATGGCCAACGTCTTCGAAACAGGATGTTCAAGTTTATCATGGAAGGACCTTGACGGCTCCTGTGACACTCGACAAATGCTTGAAATCCATTAAAGAACATGCTTTTGATAAATCTCCATACCCTGTTGTTATTACTTTTGAAGACCACCTTACGACCAAGCTTCGGTGTATTGTTGCAGAGATGGTTACCCAAATATTTGGAGATATGTTGTATTATCCAAAGTCAGAAGACCCGATGACAGAATTTCCTTCACCTGAAGCTTTAAAATATCGGATTATTATCTCAACCAAGCCACCAAAAGTAGGTGAAGGTAATGACAAGGATTCATCTGAAGAATCAGAAAAGAGTGACTGCAGTGATAAAGAAACTGAGAGCAAAACTGCAGTTGACATCAAACCCATCCAGTCAGAAGCACCTGAGTATGCGGAACTTATAACAATCCATGCTGGAAAACCACAGGGTGGTTTGAAAGAGGCTCTAGAGGTTGGTGAAAAAGTAAGACGTCTTAGTTTGAGTGAACAAAAACTAGAAAAGGCTGCTGAAGATCATGGAGCTGATATTGTTAGGTTCACACAGAGGAACATTCTAAGGATCTACCCCAAGGGAACACGAGTTACCTCCTCGAATTACAAACCGCATGTTGGGTGGATGCATGGAGCTCAAATGGTTGCGTTTAATATGCAGGGACGTGACAAGTTACTATGGTTGATGCATGGGATGTTTAGAGCCAATGGAGGGTGTGGTTACGTGAAAAAGCCTGGCTTTTTGATGCATAAGGGCCCAAATAATGAGGTTTTTTATCCTAGAAGGCACTTGATGGTGACAAAGACATTGAAGGTGAGAGGACAATTTATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

497

Amino Acids

56.47

Weight (kDa)

8.96

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 166 - 309 1.3e-52 Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y PF00387 380 - 466 5.5e-29 Phosphatidylinositol-specific phospholipase C, Y domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1070
AatII GACGTC 2 cut(s) 189, 1141
AccB7I CCANNNNNTGG 1 cut(s) 1097
AciI CCGC 5 cut(s) 148, 213, 219, 1061, 1271
AclWI GGATC 2 cut(s) 25, 1235
AcoI YGGCCR 2 cut(s) 291, 638
AcsI RAATTY 3 cut(s) 431, 458, 878
AcuI CTGAAG 3 cut(s) 912, 987, 1197
AcyI GRCGYC 2 cut(s) 186, 1138
AdeI CACNNNGTG 1 cut(s) 1097
AfaI GTAC 2 cut(s) 486, 545
AfiI CCNNNNNNNGG 7 cut(s) 219, 424, 798, 938, 1097, 1277, 1371
AjiI CACGTC 1 cut(s) 1325
AjnI CCWGG 2 cut(s) 267, 1393
AluBI AGCT 5 cut(s) 628, 796, 896, 1190, 1295
AluI AGCT 5 cut(s) 628, 796, 896, 1190, 1295
Alw21I GWGCWC 2 cut(s) 414, 1297
AlwI GGATC 2 cut(s) 25, 1235
AlwNI CAGNNNCTG 1 cut(s) 573
AoxI GGCC 7 cut(s) 270, 291, 393, 415, 466, 638, 1414
ApaI GGGCCC 1 cut(s) 1418
ApeKI GCWGC 2 cut(s) 386, 1172
ApoI RAATTY 3 cut(s) 431, 458, 878
AspLEI GCGC 2 cut(s) 306, 391
AspS9I GGNCC 6 cut(s) 346, 394, 467, 681, 1414, 1415
AsuHPI GGTGA 5 cut(s) 879, 953, 1136, 1468, 1486
AsuII TTCGAA 1 cut(s) 650
AvaII GGWCC 2 cut(s) 346, 681
BaeGI GKGCMC 2 cut(s) 537, 1418
BalI TGGCCA 1 cut(s) 640
BanII GRGCYC 2 cut(s) 1297, 1418
BauI CACGAG 1 cut(s) 1245
BbsI GAAGAC 3 cut(s) 639, 783, 870
Bbv12I GWGCWC 2 cut(s) 414, 1297
BbvI GCAGC 2 cut(s) 398, 1159
BccI CCATC 4 cut(s) 479, 811, 1043, 1447
BceAI ACGGC 3 cut(s) 430, 481, 705
BciT130I CCWGG 2 cut(s) 269, 1395
BfaI CTAG 3 cut(s) 1115, 1163, 1440
BfmI CTRYAG 4 cut(s) 384, 574, 988, 1017
BfoI RGCGCY 1 cut(s) 392
BisI GCNGC 3 cut(s) 213, 387, 1173
BlpI GCTNAGC 1 cut(s) 70
BlsI GCNGC 3 cut(s) 214, 388, 1174
Bme1390I CCNGG 2 cut(s) 269, 1395
Bme18I GGWCC 2 cut(s) 346, 681
BmgBI CACGTC 1 cut(s) 1325
BmgT120I GGNCC 6 cut(s) 346, 394, 467, 681, 1414, 1415
BmiI GGNNCC 4 cut(s) 348, 469, 693, 1416
BmrFI CCNGG 2 cut(s) 269, 1395
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 7 cut(s) 83, 286, 315, 346, 1275, 1335, 1395
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 3 cut(s) 639, 783, 870
Bpu1102I GCTNAGC 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 650
BsaAI YACGTR 1 cut(s) 1384
BsaBI GATNNNNATC 2 cut(s) 22, 914
BsaHI GRCGYC 2 cut(s) 186, 1138
BsaJI CCNNGG 1 cut(s) 1236
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 7 cut(s) 219, 424, 798, 938, 1097, 1277, 1371
Bse118I RCCGGY 2 cut(s) 215, 391
Bse1I ACTGG 2 cut(s) 556, 1039
Bse8I GATNNNNATC 2 cut(s) 22, 914
BseBI CCWGG 2 cut(s) 269, 1395
BseDI CCNNGG 1 cut(s) 1236
BseGI GGATG 5 cut(s) 306, 664, 1035, 1290, 1359
BseJI GATNNNNATC 2 cut(s) 22, 914
BseLI CCNNNNNNNGG 7 cut(s) 219, 424, 798, 938, 1097, 1277, 1371
BseMII CTCAG 4 cut(s) 84, 577, 996, 1044
BseNI ACTGG 2 cut(s) 556, 1039
BseRI GAGGAG 1 cut(s) 1246
BseSI GKGCMC 2 cut(s) 537, 1418
BseXI GCAGC 2 cut(s) 398, 1159
BshFI GGCC 7 cut(s) 272, 293, 395, 417, 468, 640, 1416
BsiHKAI GWGCWC 2 cut(s) 414, 1297
BsiSI CCGG 2 cut(s) 216, 392
BslFI GGGAC 1 cut(s) 1335
BslI CCNNNNNNNGG 7 cut(s) 219, 424, 798, 938, 1097, 1277, 1371
BsmFI GGGAC 1 cut(s) 1335
BsnI GGCC 7 cut(s) 272, 293, 395, 417, 468, 640, 1416
Bsp119I TTCGAA 1 cut(s) 650
Bsp120I GGGCCC 1 cut(s) 1414
Bsp1286I GDGCHC 4 cut(s) 414, 537, 1297, 1418
Bsp143I GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
Bsp1720I GCTNAGC 1 cut(s) 70
BspACI CCGC 5 cut(s) 148, 213, 219, 1061, 1271
BspANI GGCC 7 cut(s) 272, 293, 395, 417, 468, 640, 1416
BspCNI CTCAG 4 cut(s) 83, 576, 997, 1045
BspLI GGNNCC 4 cut(s) 348, 469, 693, 1416
BspMAI CTGCAG 3 cut(s) 388, 992, 1021
BspPI GGATC 2 cut(s) 25, 1235
BspT104I TTCGAA 1 cut(s) 650
BsrFI RCCGGY 2 cut(s) 215, 391
BsrI ACTGG 2 cut(s) 556, 1039
BssAI RCCGGY 2 cut(s) 215, 391
BssECI CCNNGG 1 cut(s) 1236
BssMI GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
BssNI GRCGYC 2 cut(s) 186, 1138
BssSI CACGAG 1 cut(s) 1245
BssT1I CCWWGG 1 cut(s) 1236
Bst2BI CACGAG 1 cut(s) 1245
Bst2UI CCWGG 2 cut(s) 269, 1395
Bst4CI ACNGT 2 cut(s) 366, 575
BstACI GRCGYC 2 cut(s) 186, 1138
BstBAI YACGTR 1 cut(s) 1384
BstBI TTCGAA 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 217, 393
BstDEI CTNAG 6 cut(s) 70, 563, 1005, 1053, 1142, 1223
BstEII GGTNACC 1 cut(s) 820
BstF5I GGATG 5 cut(s) 306, 664, 1035, 1290, 1359
BstH2I RGCGCY 1 cut(s) 392
BstHHI GCGC 2 cut(s) 306, 391
BstKTI GATC 7 cut(s) 20, 90, 162, 241, 261, 1183, 1230
BstMBI GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNI CCWGG 2 cut(s) 269, 1395
BstNSI RCATGY 2 cut(s) 737, 1277
BstPI GGTNACC 1 cut(s) 820
BstSCI CCNGG 2 cut(s) 267, 1393
BstSFI CTRYAG 4 cut(s) 384, 574, 988, 1017
BstSLI GKGCMC 2 cut(s) 537, 1418
BstV1I GCAGC 2 cut(s) 398, 1159
BstV2I GAAGAC 3 cut(s) 639, 783, 870
BstX2I RGATCY 1 cut(s) 1227
BstXI CCANNNNNNTGG 2 cut(s) 14, 833
BstYI RGATCY 1 cut(s) 1227
BsuRI GGCC 7 cut(s) 272, 293, 395, 417, 468, 640, 1416
BtrI CACGTC 1 cut(s) 1325
BtsCI GGATG 5 cut(s) 306, 664, 1035, 1290, 1359
BtsI GCAGTG 2 cut(s) 574, 997
BtsIMutI CAGTG 2 cut(s) 574, 997
Cac8I GCNNGC 2 cut(s) 217, 393
CaiI CAGNNNCTG 1 cut(s) 573
CfoI GCGC 2 cut(s) 306, 391
Cfr10I RCCGGY 2 cut(s) 215, 391
Cfr13I GGNCC 6 cut(s) 346, 394, 467, 681, 1414, 1415
Csp6I GTAC 2 cut(s) 485, 544
CviQI GTAC 2 cut(s) 485, 544
DdeI CTNAG 6 cut(s) 70, 563, 1005, 1053, 1142, 1223
DpnI GATC 7 cut(s) 19, 89, 161, 240, 260, 1182, 1229
DpnII GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
DraI TTTAAA 1 cut(s) 900
DraIII CACNNNGTG 1 cut(s) 1097
EaeI YGGCCR 2 cut(s) 291, 638
Ecl136II GAGCTC 1 cut(s) 1295
Eco130I CCWWGG 1 cut(s) 1236
Eco24I GRGCYC 2 cut(s) 1297, 1418
Eco47I GGWCC 2 cut(s) 346, 681
Eco53kI GAGCTC 1 cut(s) 1295
Eco57I CTGAAG 3 cut(s) 912, 987, 1197
Eco91I GGTNACC 1 cut(s) 820
EcoICRI GAGCTC 1 cut(s) 1295
EcoO109I RGGNCCY 2 cut(s) 681, 1414
EcoO65I GGTNACC 1 cut(s) 820
EcoRI GAATTC 2 cut(s) 431, 458
EcoRII CCWGG 2 cut(s) 267, 1393
EcoT14I CCWWGG 1 cut(s) 1236
EcoT22I ATGCAT 3 cut(s) 1290, 1350, 1410
EcoT38I GRGCYC 2 cut(s) 1297, 1418
ErhI CCWWGG 1 cut(s) 1236
FalI AAGNNNNNCTT 6 cut(s) 182, 214, 768, 800, 1382, 1414
FaqI GGGAC 1 cut(s) 1335
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 213, 387, 1173
FokI GGATG 5 cut(s) 293, 671, 1022, 1297, 1366
FriOI GRGCYC 2 cut(s) 1297, 1418
Fsp4HI GCNGC 3 cut(s) 213, 387, 1173
FspBI CTAG 3 cut(s) 1115, 1163, 1440
GlaI GCGC 2 cut(s) 305, 390
GluI GCNGC 3 cut(s) 213, 387, 1173
HaeII RGCGCY 1 cut(s) 392
HaeIII GGCC 7 cut(s) 272, 293, 395, 417, 468, 640, 1416
HapII CCGG 2 cut(s) 216, 392
HhaI GCGC 2 cut(s) 306, 391
Hin1I GRCGYC 2 cut(s) 186, 1138
Hin6I GCGC 2 cut(s) 304, 389
HinP1I GCGC 2 cut(s) 304, 389
HincII GTYRAC 1 cut(s) 1024
HindII GTYRAC 1 cut(s) 1024
HindIII AAGCTT 2 cut(s) 794, 894
HinfI GANTC 3 cut(s) 556, 959, 971
HpaII CCGG 2 cut(s) 216, 392
HphI GGTGA 5 cut(s) 879, 953, 1136, 1468, 1486
Hpy166II GTNNAC 4 cut(s) 487, 1024, 1154, 1206
Hpy188I TCNGA 8 cut(s) 28, 79, 329, 862, 909, 967, 976, 1045
Hpy188III TCNNGA 5 cut(s) 91, 157, 182, 256, 1115
Hpy8I GTNNAC 4 cut(s) 487, 1024, 1154, 1206
Hpy99I CGWCG 3 cut(s) 107, 188, 333
HpyAV CCTTC 5 cut(s) 672, 894, 938, 1437, 1465
HpyCH4III ACNGT 2 cut(s) 366, 575
HpyCH4IV ACGT 6 cut(s) 186, 251, 644, 1138, 1324, 1383
HpyCH4V TGCA 8 cut(s) 386, 812, 990, 1019, 1288, 1318, 1348, 1408
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpyF3I CTNAG 6 cut(s) 70, 563, 1005, 1053, 1142, 1223
HpySE526I ACGT 6 cut(s) 186, 251, 644, 1138, 1324, 1383
Hsp92I GRCGYC 2 cut(s) 186, 1138
HspAI GCGC 2 cut(s) 304, 389
KroI GCCGGC 2 cut(s) 215, 391
KroNI GCCGGC 2 cut(s) 217, 393
Kzo9I GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
LmnI GCTCC 5 cut(s) 330, 508, 697, 1187, 1292
Lsp1109I GCAGC 2 cut(s) 398, 1159
LweI GCATC 7 cut(s) 83, 286, 315, 346, 1275, 1335, 1395
MaeI CTAG 3 cut(s) 1115, 1163, 1440
MaeII ACGT 6 cut(s) 186, 251, 644, 1138, 1324, 1383
MaeIII GTNAC 9 cut(s) 569, 697, 820, 983, 1249, 1325, 1332, 1379, 1456
MalI GATC 7 cut(s) 19, 89, 161, 240, 260, 1182, 1229
MboI GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
MboII GAAGA 9 cut(s) 133, 190, 427, 467, 639, 788, 875, 980, 1190
MflI RGATCY 1 cut(s) 1227
MhlI GDGCHC 4 cut(s) 414, 537, 1297, 1418
MlsI TGGCCA 1 cut(s) 640
MluCI AATT 8 cut(s) 431, 439, 458, 538, 621, 878, 1261, 1484
MluNI TGGCCA 1 cut(s) 640
MmeI TCCRAC 1 cut(s) 352
Mox20I TGGCCA 1 cut(s) 640
Mph1103I ATGCAT 3 cut(s) 1290, 1350, 1410
MreI CGCCGGCG 1 cut(s) 215
MroNI GCCGGC 2 cut(s) 215, 391
MscI TGGCCA 1 cut(s) 640
MseI TTAA 5 cut(s) 594, 726, 899, 1311, 1492
MslI CAYNNNNRTG 1 cut(s) 12
Msp20I TGGCCA 1 cut(s) 640
MspI CCGG 2 cut(s) 216, 392
MspR9I CCNGG 2 cut(s) 269, 1395
MvaI CCWGG 2 cut(s) 269, 1395
MwoI GCNNNNNNNGC 1 cut(s) 392
NaeI GCCGGC 2 cut(s) 217, 393
NdeII GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
NgoMIV GCCGGC 2 cut(s) 215, 391
NlaIV GGNNCC 4 cut(s) 348, 469, 693, 1416
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 5 cut(s) 569, 697, 983, 1325, 1456
NsiI ATGCAT 3 cut(s) 1290, 1350, 1410
NspI RCATGY 2 cut(s) 737, 1277
NspV TTCGAA 1 cut(s) 650
PdiI GCCGGC 2 cut(s) 217, 393
PfeI GAWTC 3 cut(s) 556, 959, 971
PflMI CCANNNNNTGG 1 cut(s) 1097
PkrI GCNGC 3 cut(s) 214, 388, 1174
Ppu21I YACGTR 1 cut(s) 1384
PpuMI RGGWCCY 1 cut(s) 681
PsiI TTATAA 1 cut(s) 1070
Psp124BI GAGCTC 1 cut(s) 1297
Psp5II RGGWCCY 1 cut(s) 681
Psp6I CCWGG 2 cut(s) 267, 1393
PspEI GGTNACC 1 cut(s) 820
PspGI CCWGG 2 cut(s) 267, 1393
PspN4I GGNNCC 4 cut(s) 348, 469, 693, 1416
PspOMI GGGCCC 1 cut(s) 1414
PspPI GGNCC 6 cut(s) 346, 394, 467, 681, 1414, 1415
PspPPI RGGWCCY 1 cut(s) 681
PsrI GAACNNNNNNTAC 2 cut(s) 1234, 1266
PstI CTGCAG 3 cut(s) 388, 992, 1021
PstNI CAGNNNCTG 1 cut(s) 573
PsuI RGATCY 1 cut(s) 1227
RsaI GTAC 2 cut(s) 486, 545
RsaNI GTAC 2 cut(s) 485, 544
RseI CAYNNNNRTG 1 cut(s) 12
SacI GAGCTC 1 cut(s) 1297
SaqAI TTAA 5 cut(s) 594, 726, 899, 1311, 1492
SatI GCNGC 3 cut(s) 213, 387, 1173
Sau3AI GATC 7 cut(s) 17, 87, 159, 238, 258, 1180, 1227
Sau96I GGNCC 6 cut(s) 346, 394, 467, 681, 1414, 1415
ScrFI CCNGG 2 cut(s) 269, 1395
SduI GDGCHC 4 cut(s) 414, 537, 1297, 1418
SfaNI GCATC 7 cut(s) 83, 286, 315, 346, 1275, 1335, 1395
SfcI CTRYAG 4 cut(s) 384, 574, 988, 1017
SfuI TTCGAA 1 cut(s) 650
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 2 cut(s) 346, 681
SmiMI CAYNNNNRTG 1 cut(s) 12
Sse9I AATT 8 cut(s) 431, 439, 458, 538, 621, 878, 1261, 1484
SsiI CCGC 5 cut(s) 148, 213, 219, 1061, 1271
SspI AATATT 1 cut(s) 831
SspMI CTAG 3 cut(s) 1115, 1163, 1440
SstI GAGCTC 1 cut(s) 1297
StyD4I CCNGG 2 cut(s) 267, 1393
StyI CCWWGG 1 cut(s) 1236
TaaI ACNGT 2 cut(s) 366, 575
TaiI ACGT 6 cut(s) 189, 254, 647, 1141, 1327, 1386
TaqI TCGA 6 cut(s) 237, 261, 450, 650, 705, 1259
TasI AATT 8 cut(s) 431, 439, 458, 538, 621, 878, 1261, 1484
TauI GCSGC 1 cut(s) 215
TfiI GAWTC 3 cut(s) 556, 959, 971
Tru1I TTAA 5 cut(s) 594, 726, 899, 1311, 1492
Tru9I TTAA 5 cut(s) 594, 726, 899, 1311, 1492
TscAI CASTG 2 cut(s) 574, 997
TseFI GTSAC 5 cut(s) 569, 697, 983, 1325, 1456
TseI GCWGC 2 cut(s) 386, 1172
Tsp45I GTSAC 5 cut(s) 569, 697, 983, 1325, 1456
TspDTI ATGAA 3 cut(s) 468, 511, 951
TspGWI ACGGA 3 cut(s) 120, 359, 495
TspRI CASTG 2 cut(s) 574, 997
Van91I CCANNNNNTGG 1 cut(s) 1097
VpaK11BI GGWCC 2 cut(s) 346, 681
XapI RAATTY 3 cut(s) 431, 458, 878
XbaI TCTAGA 1 cut(s) 1114
XceI RCATGY 2 cut(s) 737, 1277
XspI CTAG 3 cut(s) 1115, 1163, 1440
ZraI GACGTC 2 cut(s) 187, 1139
Zsp2I ATGCAT 3 cut(s) 1290, 1350, 1410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.