Rh1BG326600

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0008969
Physical Location & Seq
Forward (+)
45893841 .. 45897418
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG326600.1

Sequence Viewer

Length: 1014 bp
ATGGGCGACGGGAATAACACCAACAACTACAAGATGTTCAAGTTCTTCAACCGCAAGTTCAAGATCAGCGAGTTGAAGCCACCTCCCGACGTCAAGGACATCTTCAACAAGTTCGCCGCCGGCGGGGACGTCATGTCCCCCGACCAGTTCCGTACGTTCTTGATCGAACACCAGTCCGAGAAATCCGTTTCTTTGGCCGATGCCAAGAGCATCCTACATGAGTTCTCGCTCCGACGACAGCATCTTACGGACCCGAAACACAAGACAGACAAGAAACAGTCCAAAGAGAACAAAACTGCCGCGCCGGCCCACCAAACACATGAGCACGGCCTCACTTTGGACGAATTCTTCAATTTCCTATTCGAGGAAGAATTCAACGGCCCCATCAAACGAGAGGTACACCATGATATGACTGCTCCGTTGTCGCATTATTTCATATATACAGGGCACAATTCGTACCTTACTGGGAATCAACTCAGCAGTGACTGTAGTGATGTGCCAATCATTAAGGCTTTGGAGAGAGGGGTCAAAGTAATTGAGCTTGATTTATGGCCAACGTCTTCGAAACAGGATGTTCAAGTTTATCATGGAAGGACCTTGACGGCTCCTGTGACACTCGTCAAATGCTTGAAATCCATTAAAGAACATGCTTTTGATAAATCTCCATACCCTGTTGTTATTACTTTTGAAGACCACCTTACGACCAAGCTTCGGTGTATAGTTGCAGAGGTTTATATTGTTGGAGTGCCTGCAGATTGTGCCAAGTACAGAACAGAAATAAAAAACGACGAATGGATACCTGTTTGGGATGAAGAGTTTACATTCGGTATGACCGTTCCAGAGCTCGCTATACTTCGGATTGAAGTTCGAGAGTATGACAGGTCTGATAAAGATGACTTTGGTGGCCAGACGTGTTTGCCGGTCTCGGAGCTAAGAACCGGGTTCCGGTCAGTCGCACTTTACGATCATAAGGTTCCAAATCACAATCGATATGCCAGTTGGACTCCACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

337

Amino Acids

38.79

Weight (kDa)

6.38

Isoelectric Point (pI)

47.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PI-PLC-X PF00388 137 - 243 3.2e-38 Phosphatidylinositol-specific phospholipase C, X domain
C2 PF00168 241 - 314 7.5e-12 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 93, 132
AccII CGCG 1 cut(s) 302
AciI CCGC 4 cut(s) 52, 117, 123, 300
AcoI YGGCCR 3 cut(s) 195, 551, 904
AcsI RAATTY 2 cut(s) 344, 371
AcyI GRCGYC 2 cut(s) 90, 129
AfaI GTAC 4 cut(s) 154, 399, 458, 767
AfiI CCNNNNNNNGG 5 cut(s) 123, 337, 364, 711, 945
AflIII ACRYGT 1 cut(s) 911
AhdI GACNNNNNGTC 1 cut(s) 133
AjiI CACGTC 1 cut(s) 912
AluBI AGCT 4 cut(s) 541, 709, 844, 931
AluI AGCT 4 cut(s) 541, 709, 844, 931
Alw21I GWGCWC 2 cut(s) 327, 846
Alw26I GTCTC 1 cut(s) 928
AlwNI CAGNNNCTG 1 cut(s) 486
AoxI GGCC 6 cut(s) 195, 306, 328, 379, 551, 904
ApoI RAATTY 2 cut(s) 344, 371
AspLEI GCGC 1 cut(s) 304
AspS9I GGNCC 4 cut(s) 250, 307, 380, 594
AsuC2I CCSGG 1 cut(s) 940
AsuII TTCGAA 1 cut(s) 563
AvaII GGWCC 2 cut(s) 250, 594
BaeGI GKGCMC 1 cut(s) 450
BalI TGGCCA 2 cut(s) 553, 906
BanII GRGCYC 1 cut(s) 846
BbsI GAAGAC 2 cut(s) 552, 696
Bbv12I GWGCWC 2 cut(s) 327, 846
BccI CCATC 1 cut(s) 392
BceAI ACGGC 3 cut(s) 343, 394, 618
BciVI GTATCC 1 cut(s) 789
BcnI CCSGG 1 cut(s) 940
BcoDI GTCTC 1 cut(s) 928
BfmI CTRYAG 2 cut(s) 487, 750
BfuI GTATCC 1 cut(s) 789
BglI GCCNNNNNGGC 1 cut(s) 305
BisI GCNGC 2 cut(s) 117, 300
BlsI GCNGC 2 cut(s) 118, 301
Bme1390I CCNGG 1 cut(s) 940
Bme18I GGWCC 2 cut(s) 250, 594
BmeRI GACNNNNNGTC 1 cut(s) 133
BmgBI CACGTC 1 cut(s) 912
BmgT120I GGNCC 4 cut(s) 250, 307, 380, 594
BmiI GGNNCC 5 cut(s) 252, 382, 606, 944, 975
BmrFI CCNGG 1 cut(s) 940
BmrI ACTGGG 1 cut(s) 474
BmsI GCATC 3 cut(s) 190, 219, 250
BmuI ACTGGG 1 cut(s) 474
BoxI GACNNNNGTC 1 cut(s) 617
BpiI GAAGAC 2 cut(s) 552, 696
Bpu14I TTCGAA 1 cut(s) 563
BpuMI CCSGG 1 cut(s) 940
Bsa29I ATCGAT 1 cut(s) 988
BsaHI GRCGYC 2 cut(s) 90, 129
BsaI GGTCTC 1 cut(s) 928
BsaWI WCCGGW 1 cut(s) 945
Bsc4I CCNNNNNNNGG 5 cut(s) 123, 337, 364, 711, 945
Bse118I RCCGGY 3 cut(s) 119, 304, 919
Bse1I ACTGG 4 cut(s) 145, 172, 469, 996
BseCI ATCGAT 1 cut(s) 988
BseGI GGATG 3 cut(s) 210, 577, 814
BseLI CCNNNNNNNGG 5 cut(s) 123, 337, 364, 711, 945
BseMII CTCAG 1 cut(s) 490
BseNI ACTGG 4 cut(s) 145, 172, 469, 996
BseSI GKGCMC 1 cut(s) 450
Bsh1236I CGCG 1 cut(s) 302
BshFI GGCC 6 cut(s) 197, 308, 330, 381, 553, 906
BshVI ATCGAT 1 cut(s) 988
BsiHKAI GWGCWC 2 cut(s) 327, 846
BsiSI CCGG 5 cut(s) 120, 305, 920, 939, 946
BsiWI CGTACG 1 cut(s) 152
BslFI GGGAC 2 cut(s) 121, 140
BslI CCNNNNNNNGG 5 cut(s) 123, 337, 364, 711, 945
BsmAI GTCTC 1 cut(s) 928
BsmFI GGGAC 2 cut(s) 121, 140
BsnI GGCC 6 cut(s) 197, 308, 330, 381, 553, 906
Bso31I GGTCTC 1 cut(s) 928
Bsp119I TTCGAA 1 cut(s) 563
Bsp1286I GDGCHC 3 cut(s) 327, 450, 846
Bsp143I GATC 3 cut(s) 63, 162, 964
BspACI CCGC 4 cut(s) 52, 117, 123, 300
BspANI GGCC 6 cut(s) 197, 308, 330, 381, 553, 906
BspCNI CTCAG 1 cut(s) 489
BspDI ATCGAT 1 cut(s) 988
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 5 cut(s) 252, 382, 606, 944, 975
BspMAI CTGCAG 1 cut(s) 754
BspT104I TTCGAA 1 cut(s) 563
BspTNI GGTCTC 1 cut(s) 928
BsrFI RCCGGY 3 cut(s) 119, 304, 919
BsrI ACTGG 4 cut(s) 145, 172, 469, 996
BssAI RCCGGY 3 cut(s) 119, 304, 919
BssMI GATC 3 cut(s) 63, 162, 964
BssNI GRCGYC 2 cut(s) 90, 129
Bst4CI ACNGT 3 cut(s) 279, 488, 835
Bst6I CTCTTC 1 cut(s) 807
BstACI GRCGYC 2 cut(s) 90, 129
BstAPI GCANNNNNTGC 1 cut(s) 758
BstBI TTCGAA 1 cut(s) 563
BstC8I GCNNGC 4 cut(s) 121, 306, 750, 846
BstDEI CTNAG 2 cut(s) 476, 932
BstENI CCTNNNNNAGG 1 cut(s) 362
BstF5I GGATG 3 cut(s) 210, 577, 814
BstFNI CGCG 1 cut(s) 302
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 3 cut(s) 66, 165, 967
BstMAI GTCTC 1 cut(s) 928
BstMBI GATC 3 cut(s) 63, 162, 964
BstMWI GCNNNNNNNGC 2 cut(s) 305, 758
BstNSI RCATGY 1 cut(s) 650
BstPAI GACNNNNGTC 1 cut(s) 617
BstSCI CCNGG 1 cut(s) 938
BstSFI CTRYAG 2 cut(s) 487, 750
BstSLI GKGCMC 1 cut(s) 450
BstUI CGCG 1 cut(s) 302
BstV2I GAAGAC 2 cut(s) 552, 696
Bsu15I ATCGAT 1 cut(s) 988
BsuI GTATCC 1 cut(s) 789
BsuRI GGCC 6 cut(s) 197, 308, 330, 381, 553, 906
BsuTUI ATCGAT 1 cut(s) 988
BtrI CACGTC 1 cut(s) 912
BtsCI GGATG 3 cut(s) 210, 577, 814
BtsI GCAGTG 1 cut(s) 487
BtsIMutI CAGTG 1 cut(s) 487
Cac8I GCNNGC 4 cut(s) 121, 306, 750, 846
CaiI CAGNNNCTG 1 cut(s) 486
CfoI GCGC 1 cut(s) 304
Cfr10I RCCGGY 3 cut(s) 119, 304, 919
Cfr13I GGNCC 4 cut(s) 250, 307, 380, 594
ClaI ATCGAT 1 cut(s) 988
Csp6I GTAC 4 cut(s) 153, 398, 457, 766
CviAII CATG 6 cut(s) 133, 218, 320, 404, 587, 647
CviQI GTAC 4 cut(s) 153, 398, 457, 766
DdeI CTNAG 2 cut(s) 476, 932
DpnI GATC 3 cut(s) 65, 164, 966
DpnII GATC 3 cut(s) 63, 162, 964
DriI GACNNNNNGTC 1 cut(s) 133
EaeI YGGCCR 3 cut(s) 195, 551, 904
Eam1104I CTCTTC 1 cut(s) 807
Eam1105I GACNNNNNGTC 1 cut(s) 133
EarI CTCTTC 1 cut(s) 807
Ecl136II GAGCTC 1 cut(s) 844
Eco24I GRGCYC 1 cut(s) 846
Eco31I GGTCTC 1 cut(s) 928
Eco47I GGWCC 2 cut(s) 250, 594
Eco53kI GAGCTC 1 cut(s) 844
EcoICRI GAGCTC 1 cut(s) 844
EcoNI CCTNNNNNAGG 1 cut(s) 362
EcoO109I RGGNCCY 1 cut(s) 594
EcoRI GAATTC 2 cut(s) 344, 371
EcoT38I GRGCYC 1 cut(s) 846
FaeI CATG 6 cut(s) 136, 221, 323, 407, 590, 650
FalI AAGNNNNNCTT 4 cut(s) 86, 118, 681, 713
FaqI GGGAC 2 cut(s) 121, 140
FatI CATG 6 cut(s) 132, 217, 319, 403, 586, 646
FauI CCCGC 1 cut(s) 116
Fnu4HI GCNGC 2 cut(s) 117, 300
FokI GGATG 3 cut(s) 197, 584, 821
FriOI GRGCYC 1 cut(s) 846
Fsp4HI GCNGC 2 cut(s) 117, 300
GlaI GCGC 1 cut(s) 303
GluI GCNGC 2 cut(s) 117, 300
HaeIII GGCC 6 cut(s) 197, 308, 330, 381, 553, 906
HapII CCGG 5 cut(s) 120, 305, 920, 939, 946
HhaI GCGC 1 cut(s) 304
Hin1I GRCGYC 2 cut(s) 90, 129
Hin1II CATG 6 cut(s) 136, 221, 323, 407, 590, 650
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HindIII AAGCTT 1 cut(s) 707
HinfI GANTC 2 cut(s) 469, 1003
HpaII CCGG 5 cut(s) 120, 305, 920, 939, 946
Hpy166II GTNNAC 2 cut(s) 400, 819
Hpy188I TCNGA 5 cut(s) 178, 233, 858, 886, 928
Hpy188III TCNNGA 5 cut(s) 61, 86, 160, 839, 869
Hpy8I GTNNAC 2 cut(s) 400, 819
Hpy99I CGWCG 4 cut(s) 11, 92, 237, 791
HpyAV CCTTC 1 cut(s) 585
HpyCH4III ACNGT 3 cut(s) 279, 488, 835
HpyCH4IV ACGT 5 cut(s) 90, 129, 155, 557, 911
HpyCH4V TGCA 2 cut(s) 725, 752
HpyF10VI GCNNNNNNNGC 2 cut(s) 305, 758
HpyF3I CTNAG 2 cut(s) 476, 932
HpySE526I ACGT 5 cut(s) 90, 129, 155, 557, 911
Hsp92I GRCGYC 2 cut(s) 90, 129
Hsp92II CATG 6 cut(s) 136, 221, 323, 407, 590, 650
HspAI GCGC 1 cut(s) 302
KroI GCCGGC 2 cut(s) 119, 304
KroNI GCCGGC 2 cut(s) 121, 306
Kzo9I GATC 3 cut(s) 63, 162, 964
LmnI GCTCC 4 cut(s) 234, 421, 610, 928
LweI GCATC 3 cut(s) 190, 219, 250
MaeII ACGT 5 cut(s) 90, 129, 155, 557, 911
MaeIII GTNAC 2 cut(s) 482, 610
MalI GATC 3 cut(s) 65, 164, 966
MboI GATC 3 cut(s) 63, 162, 964
MboII GAAGA 7 cut(s) 37, 94, 340, 380, 552, 701, 824
MhlI GDGCHC 3 cut(s) 327, 450, 846
MlsI TGGCCA 2 cut(s) 553, 906
MluCI AATT 5 cut(s) 344, 352, 371, 451, 534
MluNI TGGCCA 2 cut(s) 553, 906
MlyI GAGTC 1 cut(s) 997
MmeI TCCRAC 3 cut(s) 256, 721, 980
MnlI CCTC 6 cut(s) 93, 341, 358, 388, 515, 721
Mox20I TGGCCA 2 cut(s) 553, 906
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 2 cut(s) 119, 304
MscI TGGCCA 2 cut(s) 553, 906
MseI TTAA 2 cut(s) 507, 639
Msp20I TGGCCA 2 cut(s) 553, 906
MspI CCGG 5 cut(s) 120, 305, 920, 939, 946
MspR9I CCNGG 1 cut(s) 940
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 2 cut(s) 305, 758
NaeI GCCGGC 2 cut(s) 121, 306
NciI CCSGG 1 cut(s) 940
NdeII GATC 3 cut(s) 63, 162, 964
NgoMIV GCCGGC 2 cut(s) 119, 304
NlaIII CATG 6 cut(s) 136, 221, 323, 407, 590, 650
NlaIV GGNNCC 5 cut(s) 252, 382, 606, 944, 975
NmuCI GTSAC 2 cut(s) 482, 610
NspI RCATGY 1 cut(s) 650
NspV TTCGAA 1 cut(s) 563
PcsI WCGNNNNNNNCGW 2 cut(s) 831, 960
PdiI GCCGGC 2 cut(s) 121, 306
PfeI GAWTC 1 cut(s) 469
Pfl23II CGTACG 1 cut(s) 152
PkrI GCNGC 2 cut(s) 118, 301
PleI GAGTC 1 cut(s) 997
PpsI GAGTC 1 cut(s) 997
PpuMI RGGWCCY 1 cut(s) 594
PshAI GACNNNNGTC 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 594
PspLI CGTACG 1 cut(s) 152
PspN4I GGNNCC 5 cut(s) 252, 382, 606, 944, 975
PspPI GGNCC 4 cut(s) 250, 307, 380, 594
PspPPI RGGWCCY 1 cut(s) 594
PstI CTGCAG 1 cut(s) 754
PstNI CAGNNNCTG 1 cut(s) 486
RsaI GTAC 4 cut(s) 154, 399, 458, 767
RsaNI GTAC 4 cut(s) 153, 398, 457, 766
SacI GAGCTC 1 cut(s) 846
SaqAI TTAA 2 cut(s) 507, 639
SatI GCNGC 2 cut(s) 117, 300
Sau3AI GATC 3 cut(s) 63, 162, 964
Sau96I GGNCC 4 cut(s) 250, 307, 380, 594
SchI GAGTC 1 cut(s) 997
ScrFI CCNGG 1 cut(s) 940
SduI GDGCHC 3 cut(s) 327, 450, 846
SfaNI GCATC 3 cut(s) 190, 219, 250
SfcI CTRYAG 2 cut(s) 487, 750
SfuI TTCGAA 1 cut(s) 563
SgrAI CRCCGGYG 1 cut(s) 119
SinI GGWCC 2 cut(s) 250, 594
Sse9I AATT 5 cut(s) 344, 352, 371, 451, 534
SsiI CCGC 4 cut(s) 52, 117, 123, 300
SstI GAGCTC 1 cut(s) 846
StyD4I CCNGG 1 cut(s) 938
TaaI ACNGT 3 cut(s) 279, 488, 835
TaiI ACGT 5 cut(s) 93, 132, 158, 560, 914
TaqI TCGA 5 cut(s) 165, 363, 563, 868, 988
TasI AATT 5 cut(s) 344, 352, 371, 451, 534
TatI WGTACW 1 cut(s) 765
TauI GCSGC 2 cut(s) 119, 302
TfiI GAWTC 1 cut(s) 469
Tru1I TTAA 2 cut(s) 507, 639
Tru9I TTAA 2 cut(s) 507, 639
TscAI CASTG 1 cut(s) 487
TseFI GTSAC 2 cut(s) 482, 610
Tsp45I GTSAC 2 cut(s) 482, 610
TspDTI ATGAA 2 cut(s) 424, 825
TspGWI ACGGA 4 cut(s) 140, 175, 263, 408
TspRI CASTG 1 cut(s) 487
VpaK11BI GGWCC 2 cut(s) 250, 594
XagI CCTNNNNNAGG 1 cut(s) 362
XapI RAATTY 2 cut(s) 344, 371
XceI RCATGY 1 cut(s) 650
ZraI GACGTC 2 cut(s) 91, 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.