Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0008969

Physical Location & Seq

Genomic Coordinates Forward (+)

29882948 .. 29886335

Gene Structure 3388 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1221800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGGGTGACAGCAATGCGCCGCACCACAATTACACGATGTTCAAGTGCTTCAACCGGAAGTTCAAGGCCAGCGTGGCCGGCCCGCCGCCCGACGTCAAGGCGGTGTTCTCGAAGTTTGCGCAGGGCGGGGACCACATGTCCATCGACCAGTTCCGGAGCTTCCTGGTCGAGCACCAGGGCGAGTCGGCTGAGGCCCTCAGTATCTCCGACGCGCACACTATCCTCCTCGACTTCACGACCCACCACCCGGGAGAAGCCGCGCACCACAAGAAGCACGTGGGCGGTCTCACTCTCGAGGATTTCTTTCATTTCTTGTTTCTTGAAGAATTCAACAGTCCAATCAGAAGACAGGTACACCATGATATGAATGCTCCAATATCACATTACTTCATATATACAGGGCATAATTCCTACCTTACTGGGAATCAACTCAGTAGTGACTGCAGTGATGTCCCAATCATCACGGCATTGCAAAAGGGTGTTAAAGTAATTGAACTCGATTTGTGGCCAAACTCTGCAAAGGATGATGTTCAAGTTTATCATGGAAGGACCTTGACCGCTCCTGTGACGCTAATCAAATGCTTGAAGTCGATAAAAGAACATGCTTTTGATAAATCTCCATACCCCGTTGTTATAACTTTTGAAGATCACCTTACTCCCAATCTTCAGGCTAAAGTTGCAGAGATGGTTATCCAAACATTTGGAGACATGCTGTATTATCCGGGGGCAGAAGACCAGATGGTAGAGTTCCCATCGCCCGAATCTTTAAAAAACCGAGTTATCATCTCGACAAAACCGCCAAAAGAATGCAGCGAATCAAAGAGTTCGAAGGACAAGGGTGGTGGAAGGTCTTCATCTGAAGAGAGTGGGAGTGATCAAGAGGGTGAGGCTATCAACAATGCCAATGCAGCTGAAGCTAAATCATTCCAACAATCAGCGCCCGAGTACAAGCGTCTCATAACAATCCATGCTGGAAAACCGTCGGGTGAACTAAGGGATGCACTAGCGGTTGGTGATAAAGTTAGACGTCTAAGTTTGAGTGAACAGAAACTTGAAAAGGCGGCCGAAGATCATGGAGCTGATGTCGTAAGGTTCACACAGAAGAATGTTCTAAGGGTGTACCCGAAGGGAACAAGATTCAACTCCTCAAATTACGAACCACATGTTGGGTGGATGCATGGAGCTCAAATGGTTGCATTTAATATGCAGGGAAATGACAAATACAATTGGTTGATGCATGGGATGTTTAAAGCCAACGGGGGGTGCGGTTATCTGAAGAAGCCCGACTTTTTGATGTACAAGGGTCCGAATGGTGAGGTTTTCGATCCTAAAAAGACCTTGACCGTCGTAAAGACGTTGAAGGTGAAAGTATACATGGGAAACGGATGGCACTTGGATTTTAGCCGGACGCACTTTGATTCCTTCTCCCCTCCGGATTTCTACACAAAGGTTTTTATTGTTGGAGTGCCAGCAGATTGTGGTAAGTTCAAAACAAAGATAATTGAGGATGATTGGATACCTGTATGGGATGAAGAGTTTACATTCCCTATGACAGTTCCGGAGCTTGCCATACTCCGGTTAGAAGTCCGAGAGTATAACATGTCCGAGAAAGACGGTTTTGGTGGGCAAACGTGTCTGCCCGTCTCCGAGTTAAAACCCGGGATCAGGGCGGTCCCCCTGTATGATGAAAAGGGGGAGAAACTCAAATCTGTAAAGCTTCTAATAAGGTTTCAGTTCGAGAACGCACTCTCCCTTACAACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933

Pfam Domains

Protein Families

C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

588

Amino Acids

65.88

Weight (kDa)

6.5

Isoelectric Point (pI)

39.83

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI-PLC-X	PF00388	122 - 265	4.5e-53	Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y	PF00387	344 - 430	1.5e-28	Phosphatidylinositol-specific phospholipase C, Y domain
C2	PF00168	452 - 554	1.1e-12	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40116
fragaria_vesca	FvH4_7g24470 FvH4_7g24470 FvH4_7g24470 FvH4_7g24470
malus_domestica	MD01G1152900.v1.1 MD07G1221800.v1.1
prunus_persica	Prupe.2G250000_v2.0.a1
pyrus_communis	pycom01g17050 pycom07g20030
rosa_chinensis	RchiOBHm_Chr1g0370201
rosa_laevigata	RLG00000027068
rosa_multiflora	Rmu_sc0018500.1_g000002 Rmu_sc0041308.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286740 Rroxscaffold_4G00287270
rosa_rugosa	Rorug01G0354600
rosa_samantha	Rh1AG363400 Rh1BG326600 Rh1CG339900 Rh1DG357000
rosa_wichuraiana	Rw1G031980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	635
AatII	GACGTC	2 cut(s)	96, 1030
Acc16I	TGCGCA	1 cut(s)	120
AccB7I	CCANNNNNTGG	1 cut(s)	1166
AccBSI	CCGCTC	1 cut(s)	560
AccI	GTMKAC	1 cut(s)	1371
AccII	CGCG	2 cut(s)	212, 260
AccIII	TCCGGA	3 cut(s)	153, 1432, 1558
AclWI	GGATC	2 cut(s)	1319, 1670
AcoI	YGGCCR	3 cut(s)	75, 506, 1062
AcsI	RAATTY	1 cut(s)	326
AcuI	CTGAAG	4 cut(s)	650, 879, 933, 1295
AcvI	CACGTG	1 cut(s)	277
AcyI	GRCGYC	2 cut(s)	93, 1027
AfaI	GTAC	4 cut(s)	354, 947, 1121, 1298
AfiI	CCNNNNNNNGG	5 cut(s)	247, 1166, 1260, 1575, 1665
AflIII	ACRYGT	4 cut(s)	135, 1162, 1599, 1631
AhdI	GACNNNNNGTC	1 cut(s)	136
AjnI	CCWGG	2 cut(s)	162, 174
AjuI	GAANNNNNNNTTGG	2 cut(s)	1149, 1181
AloI	GAACNNNNNNTCC	1 cut(s)	1733
AluBI	AGCT	7 cut(s)	159, 911, 917, 1079, 1184, 1564, 1717
AluI	AGCT	7 cut(s)	159, 911, 917, 1079, 1184, 1564, 1717
Alw21I	GWGCWC	2 cut(s)	174, 1186
Alw26I	GTCTC	4 cut(s)	290, 699, 959, 1648
AlwI	GGATC	2 cut(s)	1319, 1670
Ama87I	CYCGRG	4 cut(s)	247, 293, 941, 1658
Aor13HI	TCCGGA	3 cut(s)	153, 1432, 1558
AoxI	GGCC	6 cut(s)	66, 75, 79, 192, 506, 1062
ApeKI	GCWGC	2 cut(s)	810, 908
ApoI	RAATTY	1 cut(s)	326
Asp700I	GAANNNNTTC	1 cut(s)	850
AspLEI	GCGC	5 cut(s)	19, 121, 214, 262, 940
AspS9I	GGNCC	6 cut(s)	80, 130, 193, 549, 1304, 1672
AsuC2I	CCSGG	5 cut(s)	248, 249, 723, 1659, 1660
AsuHPI	GGTGA	7 cut(s)	17, 641, 896, 998, 1025, 1325, 1375
AsuII	TTCGAA	1 cut(s)	827
AvaI	CYCGRG	4 cut(s)	247, 293, 941, 1658
AvaII	GGWCC	4 cut(s)	130, 549, 1304, 1672
BalI	TGGCCA	1 cut(s)	508
BanII	GRGCYC	1 cut(s)	1186
BbrPI	CACGTG	1 cut(s)	277
BbsI	GAAGAC	3 cut(s)	352, 738, 843
Bbv12I	GWGCWC	2 cut(s)	174, 1186
BbvCI	CCTCAGC	1 cut(s)	189
BbvI	GCAGC	2 cut(s)	822, 920
BccI	CCATC	5 cut(s)	149, 679, 733, 760, 1380
BceAI	ACGGC	1 cut(s)	480
BciT130I	CCWGG	2 cut(s)	164, 176
BciVI	GTATCC	1 cut(s)	1509
BclI	TGATCA	1 cut(s)	874
BcnI	CCSGG	5 cut(s)	248, 249, 723, 1659, 1660
BcoDI	GTCTC	4 cut(s)	290, 699, 959, 1648
BfaI	CTAG	1 cut(s)	1004
BfmI	CTRYAG	1 cut(s)	442
BfoI	RGCGCY	1 cut(s)	941
BfuI	GTATCC	1 cut(s)	1509
BglI	GCCNNNNNGGC	1 cut(s)	74
BisI	GCNGC	6 cut(s)	20, 86, 258, 811, 909, 1062
BlsI	GCNGC	6 cut(s)	21, 87, 259, 812, 910, 1063
Bme1390I	CCNGG	7 cut(s)	164, 176, 248, 249, 723, 1659, 1660
Bme18I	GGWCC	4 cut(s)	130, 549, 1304, 1672
BmeRI	GACNNNNNGTC	1 cut(s)	136
BmeT110I	CYCGRG	4 cut(s)	247, 293, 941, 1658
BmgT120I	GGNCC	6 cut(s)	80, 130, 193, 549, 1304, 1672
BmiI	GGNNCC	3 cut(s)	131, 1305, 1674
BmrFI	CCNGG	7 cut(s)	164, 176, 248, 249, 723, 1659, 1660
BmrI	ACTGGG	1 cut(s)	429
BmsI	GCATC	3 cut(s)	988, 1164, 1224
BmuI	ACTGGG	1 cut(s)	429
BpiI	GAAGAC	3 cut(s)	352, 738, 843
Bpu10I	CCTNAGC	1 cut(s)	189
Bpu14I	TTCGAA	1 cut(s)	827
BpuMI	CCSGG	5 cut(s)	248, 249, 723, 1659, 1660
BsaAI	YACGTR	1 cut(s)	277
BsaBI	GATNNNNATC	1 cut(s)	689
BsaHI	GRCGYC	2 cut(s)	93, 1027
BsaI	GGTCTC	1 cut(s)	290
BsaJI	CCNNGG	4 cut(s)	175, 247, 722, 1658
BsaWI	WCCGGW	5 cut(s)	54, 153, 1432, 1558, 1575
BsaXI	ACNNNNNCTCC	4 cut(s)	1068, 1098, 1733, 1763
Bsc4I	CCNNNNNNNGG	5 cut(s)	247, 1166, 1260, 1575, 1665
Bse118I	RCCGGY	1 cut(s)	77
Bse1I	ACTGG	2 cut(s)	148, 424
Bse3DI	GCAATG	2 cut(s)	19, 467
Bse8I	GATNNNNATC	1 cut(s)	689
BseAI	TCCGGA	3 cut(s)	153, 1432, 1558
BseBI	CCWGG	2 cut(s)	164, 176
BseDI	CCNNGG	4 cut(s)	175, 247, 722, 1658
BseGI	GGATG	7 cut(s)	529, 1003, 1179, 1248, 1391, 1513, 1534
BseJI	GATNNNNATC	1 cut(s)	689
BseLI	CCNNNNNNNGG	5 cut(s)	247, 1166, 1260, 1575, 1665
BseMI	GCAATG	2 cut(s)	19, 467
BseMII	CTCAG	3 cut(s)	180, 211, 445
BseNI	ACTGG	2 cut(s)	148, 424
BseRI	GAGGAG	2 cut(s)	215, 1135
BseX3I	CGGCCG	1 cut(s)	1062
BseXI	GCAGC	2 cut(s)	822, 920
Bsh1236I	CGCG	2 cut(s)	212, 260
Bsh1285I	CGRYCG	1 cut(s)	1065
BshFI	GGCC	6 cut(s)	68, 77, 81, 194, 508, 1064
BsiEI	CGRYCG	1 cut(s)	1065
BsiHKAI	GWGCWC	2 cut(s)	174, 1186
BsiHKCI	CYCGRG	4 cut(s)	247, 293, 941, 1658
BslFI	GGGAC	3 cut(s)	143, 437, 1658
BslI	CCNNNNNNNGG	5 cut(s)	247, 1166, 1260, 1575, 1665
BsmAI	GTCTC	4 cut(s)	290, 699, 959, 1648
BsmBI	CGTCTC	2 cut(s)	959, 1648
BsmFI	GGGAC	3 cut(s)	143, 437, 1658
BsmI	GAATGC	2 cut(s)	373, 812
BsnI	GGCC	6 cut(s)	68, 77, 81, 194, 508, 1064
Bso31I	GGTCTC	1 cut(s)	290
BsoBI	CYCGRG	4 cut(s)	247, 293, 941, 1658
Bsp119I	TTCGAA	1 cut(s)	827
Bsp1286I	GDGCHC	2 cut(s)	174, 1186
Bsp13I	TCCGGA	3 cut(s)	153, 1432, 1558
Bsp1407I	TGTACA	1 cut(s)	1296
Bsp143I	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
BspANI	GGCC	6 cut(s)	68, 77, 81, 194, 508, 1064
BspCNI	CTCAG	3 cut(s)	181, 210, 444
BspEI	TCCGGA	3 cut(s)	153, 1432, 1558
BspFNI	CGCG	2 cut(s)	212, 260
BspLI	GGNNCC	3 cut(s)	131, 1305, 1674
BspMAI	CTGCAG	1 cut(s)	446
BspPI	GGATC	2 cut(s)	1319, 1670
BspT104I	TTCGAA	1 cut(s)	827
BspTNI	GGTCTC	1 cut(s)	290
BsrBI	CCGCTC	1 cut(s)	560
BsrDI	GCAATG	2 cut(s)	19, 467
BsrFI	RCCGGY	1 cut(s)	77
BsrGI	TGTACA	1 cut(s)	1296
BsrI	ACTGG	2 cut(s)	148, 424
BssAI	RCCGGY	1 cut(s)	77
BssECI	CCNNGG	4 cut(s)	175, 247, 722, 1658
BssMI	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
BssNAI	GTATAC	1 cut(s)	1372
BssNI	GRCGYC	2 cut(s)	93, 1027
Bst1107I	GTATAC	1 cut(s)	1372
Bst2UI	CCWGG	2 cut(s)	164, 176
Bst4CI	ACNGT	5 cut(s)	335, 981, 1345, 1555, 1616
Bst6I	CTCTTC	2 cut(s)	855, 1527
BstACI	GRCGYC	2 cut(s)	93, 1027
BstAUI	TGTACA	1 cut(s)	1296
BstBAI	YACGTR	1 cut(s)	277
BstBI	TTCGAA	1 cut(s)	827
BstC8I	GCNNGC	5 cut(s)	70, 79, 83, 1470, 1566
BstDEI	CTNAG	6 cut(s)	189, 197, 431, 992, 1031, 1112
BstF5I	GGATG	7 cut(s)	529, 1003, 1179, 1248, 1391, 1513, 1534
BstFNI	CGCG	2 cut(s)	212, 260
BstH2I	RGCGCY	1 cut(s)	941
BstHHI	GCGC	5 cut(s)	19, 121, 214, 262, 940
BstKTI	GATC	5 cut(s)	649, 877, 1072, 1327, 1665
BstMAI	GTCTC	4 cut(s)	290, 699, 959, 1648
BstMBI	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
BstMCI	CGRYCG	1 cut(s)	1065
BstMWI	GCNNNNNNNGC	5 cut(s)	74, 78, 677, 908, 914
BstNI	CCWGG	2 cut(s)	164, 176
BstNSI	RCATGY	5 cut(s)	139, 605, 712, 1166, 1603
BstSCI	CCNGG	7 cut(s)	162, 174, 246, 247, 721, 1657, 1658
BstSFI	CTRYAG	1 cut(s)	442
BstUI	CGCG	2 cut(s)	212, 260
BstV1I	GCAGC	2 cut(s)	822, 920
BstV2I	GAAGAC	3 cut(s)	352, 738, 843
BstXI	CCANNNNNNTGG	1 cut(s)	701
BstZ17I	GTATAC	1 cut(s)	1372
BstZI	CGGCCG	1 cut(s)	1062
BsuI	GTATCC	1 cut(s)	1509
BsuRI	GGCC	6 cut(s)	68, 77, 81, 194, 508, 1064
BtgZI	GCGATG	1 cut(s)	738
BtsCI	GGATG	7 cut(s)	529, 1003, 1179, 1248, 1391, 1513, 1534
BtsI	GCAGTG	1 cut(s)	451
BtsIMutI	CAGTG	1 cut(s)	451
Cac8I	GCNNGC	5 cut(s)	70, 79, 83, 1470, 1566
CfoI	GCGC	5 cut(s)	19, 121, 214, 262, 940
Cfr10I	RCCGGY	1 cut(s)	77
Cfr13I	GGNCC	6 cut(s)	80, 130, 193, 549, 1304, 1672
Cfr9I	CCCGGG	2 cut(s)	247, 1658
CseI	GACGC	4 cut(s)	218, 577, 941, 1417
Csp6I	GTAC	4 cut(s)	353, 946, 1120, 1297
CspCI	CAANNNNNGTGG	2 cut(s)	823, 858
CviQI	GTAC	4 cut(s)	353, 946, 1120, 1297
DdeI	CTNAG	6 cut(s)	189, 197, 431, 992, 1031, 1112
DpnI	GATC	5 cut(s)	648, 876, 1071, 1326, 1664
DpnII	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
DraI	TTTAAA	2 cut(s)	768, 1249
DriI	GACNNNNNGTC	1 cut(s)	136
EaeI	YGGCCR	3 cut(s)	75, 506, 1062
EagI	CGGCCG	1 cut(s)	1062
Eam1104I	CTCTTC	2 cut(s)	855, 1527
Eam1105I	GACNNNNNGTC	1 cut(s)	136
EarI	CTCTTC	2 cut(s)	855, 1527
Ecl136II	GAGCTC	1 cut(s)	1184
EclXI	CGGCCG	1 cut(s)	1062
Eco24I	GRGCYC	1 cut(s)	1186
Eco31I	GGTCTC	1 cut(s)	290
Eco47I	GGWCC	4 cut(s)	130, 549, 1304, 1672
Eco52I	CGGCCG	1 cut(s)	1062
Eco53kI	GAGCTC	1 cut(s)	1184
Eco57I	CTGAAG	4 cut(s)	650, 879, 933, 1295
Eco72I	CACGTG	1 cut(s)	277
Eco88I	CYCGRG	4 cut(s)	247, 293, 941, 1658
EcoICRI	GAGCTC	1 cut(s)	1184
EcoO109I	RGGNCCY	2 cut(s)	193, 549
EcoRI	GAATTC	1 cut(s)	326
EcoRII	CCWGG	2 cut(s)	162, 174
EcoT22I	ATGCAT	2 cut(s)	1179, 1239
EcoT38I	GRGCYC	1 cut(s)	1186
Esp3I	CGTCTC	2 cut(s)	959, 1648
FalI	AAGNNNNNCTT	4 cut(s)	636, 668, 1271, 1303
FaqI	GGGAC	3 cut(s)	143, 437, 1658
FauI	CCCGC	2 cut(s)	90, 119
FbaI	TGATCA	1 cut(s)	874
FblI	GTMKAC	1 cut(s)	1371
Fnu4HI	GCNGC	6 cut(s)	20, 86, 258, 811, 909, 1062
FokI	GGATG	7 cut(s)	536, 1010, 1186, 1255, 1398, 1520, 1541
FriOI	GRGCYC	1 cut(s)	1186
FseI	GGCCGGCC	1 cut(s)	81
Fsp4HI	GCNGC	6 cut(s)	20, 86, 258, 811, 909, 1062
FspBI	CTAG	1 cut(s)	1004
FspI	TGCGCA	1 cut(s)	120
GlaI	GCGC	5 cut(s)	18, 120, 213, 261, 939
GluI	GCNGC	6 cut(s)	20, 86, 258, 811, 909, 1062
HaeII	RGCGCY	1 cut(s)	941
HaeIII	GGCC	6 cut(s)	68, 77, 81, 194, 508, 1064
HgaI	GACGC	4 cut(s)	218, 577, 941, 1417
HhaI	GCGC	5 cut(s)	19, 121, 214, 262, 940
Hin1I	GRCGYC	2 cut(s)	93, 1027
Hin6I	GCGC	5 cut(s)	17, 119, 212, 260, 938
HinP1I	GCGC	5 cut(s)	17, 119, 212, 260, 938
HindIII	AAGCTT	1 cut(s)	1715
HinfI	GANTC	6 cut(s)	182, 424, 761, 815, 1137, 1418
HphI	GGTGA	7 cut(s)	17, 641, 896, 998, 1025, 1325, 1375
Hpy166II	GTNNAC	7 cut(s)	355, 989, 1043, 1095, 1120, 1372, 1539
Hpy188I	TCNGA	8 cut(s)	208, 344, 859, 1275, 1308, 1589, 1606, 1648
Hpy8I	GTNNAC	7 cut(s)	355, 989, 1043, 1095, 1120, 1372, 1539
Hpy99I	CGWCG	4 cut(s)	95, 212, 985, 1349
HpyAV	CCTTC	6 cut(s)	540, 823, 840, 1120, 1354, 1432
HpyCH4III	ACNGT	5 cut(s)	335, 981, 1345, 1555, 1616
HpyCH4IV	ACGT	5 cut(s)	93, 276, 1027, 1355, 1631
HpyF10VI	GCNNNNNNNGC	5 cut(s)	74, 78, 677, 908, 914
HpyF3I	CTNAG	6 cut(s)	189, 197, 431, 992, 1031, 1112
HpySE526I	ACGT	5 cut(s)	93, 276, 1027, 1355, 1631
Hsp92I	GRCGYC	2 cut(s)	93, 1027
HspAI	GCGC	5 cut(s)	17, 119, 212, 260, 938
Kpn2I	TCCGGA	3 cut(s)	153, 1432, 1558
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
Ksp22I	TGATCA	1 cut(s)	874
Kzo9I	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
LmnI	GCTCC	6 cut(s)	156, 376, 565, 1076, 1181, 1561
Lsp1109I	GCAGC	2 cut(s)	822, 920
LweI	GCATC	3 cut(s)	988, 1164, 1224
MaeI	CTAG	1 cut(s)	1004
MaeII	ACGT	5 cut(s)	93, 276, 1027, 1355, 1631
MaeIII	GTNAC	3 cut(s)	5, 437, 565
MalI	GATC	5 cut(s)	648, 876, 1071, 1326, 1664
MbiI	CCGCTC	1 cut(s)	560
MboI	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
MfeI	CAATTG	1 cut(s)	1225
MhlI	GDGCHC	2 cut(s)	174, 1186
MlsI	TGGCCA	1 cut(s)	508
MluCI	AATT	7 cut(s)	28, 326, 406, 489, 1150, 1225, 1500
MluNI	TGGCCA	1 cut(s)	508
MlyI	GAGTC	1 cut(s)	191
MmeI	TCCRAC	3 cut(s)	231, 952, 1441
Mox20I	TGGCCA	1 cut(s)	508
Mph1103I	ATGCAT	2 cut(s)	1179, 1239
MroI	TCCGGA	3 cut(s)	153, 1432, 1558
MroNI	GCCGGC	1 cut(s)	77
MroXI	GAANNNNTTC	1 cut(s)	850
MscI	TGGCCA	1 cut(s)	508
MseI	TTAA	5 cut(s)	483, 767, 1200, 1248, 1652
Msp20I	TGGCCA	1 cut(s)	508
MspA1I	CMGCKG	1 cut(s)	911
MspR9I	CCNGG	7 cut(s)	164, 176, 248, 249, 723, 1659, 1660
MunI	CAATTG	1 cut(s)	1225
Mva1269I	GAATGC	2 cut(s)	373, 812
MvaI	CCWGG	2 cut(s)	164, 176
MvnI	CGCG	2 cut(s)	212, 260
MwoI	GCNNNNNNNGC	5 cut(s)	74, 78, 677, 908, 914
NaeI	GCCGGC	1 cut(s)	79
NciI	CCSGG	5 cut(s)	248, 249, 723, 1659, 1660
NdeII	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
NgoMIV	GCCGGC	1 cut(s)	77
NlaIV	GGNNCC	3 cut(s)	131, 1305, 1674
NmuCI	GTSAC	3 cut(s)	5, 437, 565
NsbI	TGCGCA	1 cut(s)	120
NsiI	ATGCAT	2 cut(s)	1179, 1239
NspI	RCATGY	5 cut(s)	139, 605, 712, 1166, 1603
NspV	TTCGAA	1 cut(s)	827
PaeR7I	CTCGAG	1 cut(s)	293
PciI	ACATGT	3 cut(s)	135, 1162, 1599
PctI	GAATGC	2 cut(s)	373, 812
PdiI	GCCGGC	1 cut(s)	79
PdmI	GAANNNNTTC	1 cut(s)	850
PfeI	GAWTC	5 cut(s)	424, 761, 815, 1137, 1418
PflMI	CCANNNNNTGG	1 cut(s)	1166
PkrI	GCNGC	6 cut(s)	21, 87, 259, 812, 910, 1063
PleI	GAGTC	1 cut(s)	190
PmaCI	CACGTG	1 cut(s)	277
PmlI	CACGTG	1 cut(s)	277
PpsI	GAGTC	1 cut(s)	190
Ppu21I	YACGTR	1 cut(s)	277
PpuMI	RGGWCCY	1 cut(s)	549
PscI	ACATGT	3 cut(s)	135, 1162, 1599
PsiI	TTATAA	1 cut(s)	635
Psp124BI	GAGCTC	1 cut(s)	1186
Psp5II	RGGWCCY	1 cut(s)	549
Psp6I	CCWGG	2 cut(s)	162, 174
PspCI	CACGTG	1 cut(s)	277
PspGI	CCWGG	2 cut(s)	162, 174
PspN4I	GGNNCC	3 cut(s)	131, 1305, 1674
PspPI	GGNCC	6 cut(s)	80, 130, 193, 549, 1304, 1672
PspPPI	RGGWCCY	1 cut(s)	549
PstI	CTGCAG	1 cut(s)	446
PvuII	CAGCTG	1 cut(s)	911
RigI	GGCCGGCC	1 cut(s)	81
RsaI	GTAC	4 cut(s)	354, 947, 1121, 1298
RsaNI	GTAC	4 cut(s)	353, 946, 1120, 1297
SacI	GAGCTC	1 cut(s)	1186
SaqAI	TTAA	5 cut(s)	483, 767, 1200, 1248, 1652
SatI	GCNGC	6 cut(s)	20, 86, 258, 811, 909, 1062
Sau3AI	GATC	5 cut(s)	646, 874, 1069, 1324, 1662
Sau96I	GGNCC	6 cut(s)	80, 130, 193, 549, 1304, 1672
SchI	GAGTC	1 cut(s)	191
ScrFI	CCNGG	7 cut(s)	164, 176, 248, 249, 723, 1659, 1660
SduI	GDGCHC	2 cut(s)	174, 1186
SfaNI	GCATC	3 cut(s)	988, 1164, 1224
SfcI	CTRYAG	1 cut(s)	442
SfiI	GGCCNNNNNGGCC	1 cut(s)	74
Sfr274I	CTCGAG	1 cut(s)	293
SfuI	TTCGAA	1 cut(s)	827
SinI	GGWCC	4 cut(s)	130, 549, 1304, 1672
SlaI	CTCGAG	1 cut(s)	293
SmaI	CCCGGG	2 cut(s)	249, 1660
SmlI	CTYRAG	1 cut(s)	293
SmoI	CTYRAG	1 cut(s)	293
Sse9I	AATT	7 cut(s)	28, 326, 406, 489, 1150, 1225, 1500
SspMI	CTAG	1 cut(s)	1004
SstI	GAGCTC	1 cut(s)	1186
StyD4I	CCNGG	7 cut(s)	162, 174, 246, 247, 721, 1657, 1658
TaaI	ACNGT	5 cut(s)	335, 981, 1345, 1555, 1616
TaiI	ACGT	5 cut(s)	96, 279, 1030, 1358, 1634
TasI	AATT	7 cut(s)	28, 326, 406, 489, 1150, 1225, 1500
TatI	WGTACW	2 cut(s)	945, 1296
TauI	GCSGC	4 cut(s)	22, 88, 260, 1064
TfiI	GAWTC	5 cut(s)	424, 761, 815, 1137, 1418
Tru1I	TTAA	5 cut(s)	483, 767, 1200, 1248, 1652
Tru9I	TTAA	5 cut(s)	483, 767, 1200, 1248, 1652
TscAI	CASTG	1 cut(s)	451
TseFI	GTSAC	3 cut(s)	5, 437, 565
TseI	GCWGC	2 cut(s)	810, 908
Tsp45I	GTSAC	3 cut(s)	5, 437, 565
TspDTI	ATGAA	6 cut(s)	296, 379, 380, 843, 1545, 1701
TspGWI	ACGGA	1 cut(s)	1398
TspMI	CCCGGG	2 cut(s)	247, 1658
TspRI	CASTG	1 cut(s)	451
Van91I	CCANNNNNTGG	1 cut(s)	1166
VpaK11BI	GGWCC	4 cut(s)	130, 549, 1304, 1672
XapI	RAATTY	1 cut(s)	326
XceI	RCATGY	5 cut(s)	139, 605, 712, 1166, 1603
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1167
XhoI	CTCGAG	1 cut(s)	293
XmaI	CCCGGG	2 cut(s)	247, 1658
XmiI	GTMKAC	1 cut(s)	1371
XmnI	GAANNNNTTC	1 cut(s)	850
XspI	CTAG	1 cut(s)	1004
ZraI	GACGTC	2 cut(s)	94, 1028
Zsp2I	ATGCAT	2 cut(s)	1179, 1239

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.