Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008969

Physical Location & Seq

Genomic Coordinates Forward (+)

26123173 .. 26126501

Gene Structure 3329 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1152900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1737 bp

ATGGGGGACAGCAATGCACCGCACCACAATTACAAGATGTTCAAGTGCTTCAACCGGAAGTTCAAGGCTAGCGAGGCCGGCCCGCCGCCCGACGTCAAGGCGGTGTTCGCCAAGTACGCTCAGGGCGGGGACCGGATGTCCATCGACCAGTTCCGGACCTTTCTGGTCGAGCACCAGGGCGAGTCGGCCCTCACAATCTCCGACGCCCACACTATCCTCCTCCAGTTCACGACCCGCCACCCGGGCGAGGCCGCGCAGCACAAGAAGCACGTGGGGGGACTCTCTCTCCAGGATTTCTTTCATTTCTTGTTCCTTGAAGAGTTCAACGCCCCAATCAGAAGTCAGGTACACCATGATATGAATGCTCCGGTATCACATTACTTCATATACACAGGGCATAATTCCTACCTTACTGGGAATCAACTGAGTAGTGATTGCAGCGATGTCCCAATCATCAAGGCATTGCAAAAGGGTGTTAAAGTAATTGAACTTGATCTGTGGCCAAACTCTGCAAAAGATGATGTTCAAGTTTATCATGGAAGGACCTTGACCGCTCCTGTGACGCTAATCAAATGCTTGAAGTCGATAAAAGAACATGCTTTTGATAAATCACCATACCCCGTTGTTATAACTTTTGAAGATCACCTCACTCCCAAGCTTCAGGCTAAAGTTGCAGAGTTAGTTATCAAAACATTTGGAGACACGCTGCATTATCCGGAGGCAGAAGACCAAATGGTAGAATTCCCATCACCCGAATCTTTAAAAAACCGAATTATTATCTCAACAAAACCGCCAAAAGAATGTAGCGAATCAAAGAGTTCGAAGGGCAAGGGTAGTGGAAGGTCTTCATCTGAAGAGAGTGGCAGTGATCAAGATGAAGAGGCTATCCACAGTGCCAATGCAGCTGAAGCCAATTCATACCAACAATCAGCGCCTGAGTACAAGCGTCTCATAACAATCCACGCTGGAAAACCATCAGGTGAATTAAAGGATGCTCTAGCAGTTGGTGATAAAGTTAGACGTCTTAGTTTGAGTGAACAAAAACTTGAAAAGGCGGCTGAAGATCATGGAACTGATGTCGTAAGGTTCACACAGAAGAATGTTCTAAGGGTGTACCCGAAAGGAACACGATTCAACTCCTCAAATTACGAACCACATGTTGGCTGGATGCATGGAGCTCAAATGGTTGCATTTAATATGCAGGGAAAGGACAAGTACAATTGGCTGATGCATGGGATGTTTAAAGCCAACGGAGGGTGTGGTTACTTAAAAAAGCCGGACTTTTTGATGTGCAAGGGTCCGAAAGGTGAGGTCTTCGATCCTAAAAACGCCTTGAAAGTGAAAAAGACATTGAAGGTGAAAATATACATGGGAAATGGATGGCACTTGGATTTTAGCCGGACGCACTTTGATTCCTTCTCCCCACCCGACTTCTACACAAAGGTCTATATTGTTGGAGTGCCAGCAGATTGTGGTAAATTCAAAACAAAAATAATTGAGGATGATTGGATACCTGTATGGGACGAAGAGTTTGCATTCCCTATGACTGTTCCCGAGCTTGCCATACTCCGGTTAGAAGTCCGGGAGTATGACAGGTCCGAGAAAGATGATTTTGGCGGGCAGACGTGTCTGCCCGTCTCCGAGCTAAAGCCCGGGATCAGGGCAGTCCCCCTGTATGATAAGAAGGGGGAGAAATACAAATCTGTAAGGCTTCTAATAAGGTTTCAGTTTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933

Pfam Domains

Protein Families

C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

579

Amino Acids

65.22

Weight (kDa)

8.19

Isoelectric Point (pI)

42.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI-PLC-X	PF00388	120 - 263	4.7e-52	Phosphatidylinositol-specific phospholipase C, X domain
PI-PLC-Y	PF00387	342 - 428	2e-28	Phosphatidylinositol-specific phospholipase C, Y domain
C2	PF00168	450 - 552	1.8e-14	C2 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008969)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40116
fragaria_vesca	FvH4_7g24470 FvH4_7g24470 FvH4_7g24470 FvH4_7g24470
malus_domestica	MD01G1152900.v1.1 MD07G1221800.v1.1
prunus_persica	Prupe.2G250000_v2.0.a1
pyrus_communis	pycom01g17050 pycom07g20030
rosa_chinensis	RchiOBHm_Chr1g0370201
rosa_laevigata	RLG00000027068
rosa_multiflora	Rmu_sc0018500.1_g000002 Rmu_sc0041308.1_g000001
rosa_roxburghii	Rroxscaffold_4G00286740 Rroxscaffold_4G00287270
rosa_rugosa	Rorug01G0354600
rosa_samantha	Rh1AG363400 Rh1BG326600 Rh1CG339900 Rh1DG357000
rosa_wichuraiana	Rw1G031980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	629
AatII	GACGTC	2 cut(s)	96, 1024
AccB7I	CCANNNNNTGG	1 cut(s)	1160
AccBSI	CCGCTC	1 cut(s)	554
AccII	CGCG	1 cut(s)	254
AccIII	TCCGGA	2 cut(s)	153, 715
AclWI	GGATC	2 cut(s)	1313, 1664
AcoI	YGGCCR	1 cut(s)	500
AcsI	RAATTY	2 cut(s)	740, 1478
AcuI	CTGAAG	4 cut(s)	644, 873, 927, 1080
AcvI	CACGTG	1 cut(s)	271
AcyI	GRCGYC	3 cut(s)	93, 204, 1021
AfaI	GTAC	5 cut(s)	116, 348, 941, 1115, 1217
AfiI	CCNNNNNNNGG	6 cut(s)	241, 247, 1160, 1254, 1569, 1659
AflIII	ACRYGT	2 cut(s)	1156, 1625
AhdI	GACNNNNNGTC	1 cut(s)	136
AjiI	CACGTC	1 cut(s)	1626
AjnI	CCWGG	2 cut(s)	174, 288
AjuI	GAANNNNNNNTTGG	2 cut(s)	1143, 1175
AluBI	AGCT	5 cut(s)	658, 905, 1178, 1558, 1645
AluI	AGCT	5 cut(s)	658, 905, 1178, 1558, 1645
Alw21I	GWGCWC	2 cut(s)	174, 1180
Alw26I	GTCTC	3 cut(s)	693, 953, 1642
AlwI	GGATC	2 cut(s)	1313, 1664
AlwNI	CAGNNNCTG	1 cut(s)	935
Ama87I	CYCGRG	3 cut(s)	241, 1553, 1652
Aor13HI	TCCGGA	2 cut(s)	153, 715
AoxI	GGCC	5 cut(s)	75, 79, 186, 249, 500
ApeKI	GCWGC	4 cut(s)	256, 438, 706, 902
ApoI	RAATTY	2 cut(s)	740, 1478
ArsI	GACNNNNNNTTYG	2 cut(s)	1514, 1546
Asp700I	GAANNNNTTC	1 cut(s)	844
AspLEI	GCGC	2 cut(s)	256, 934
AspS9I	GGNCC	7 cut(s)	80, 130, 156, 187, 543, 1298, 1596
AsuC2I	CCSGG	5 cut(s)	242, 243, 1583, 1653, 1654
AsuHPI	GGTGA	7 cut(s)	603, 635, 741, 992, 1019, 1319, 1369
AsuII	TTCGAA	1 cut(s)	821
AsuNHI	GCTAGC	1 cut(s)	68
AvaI	CYCGRG	3 cut(s)	241, 1553, 1652
AvaII	GGWCC	5 cut(s)	130, 156, 543, 1298, 1596
BalI	TGGCCA	1 cut(s)	502
BanII	GRGCYC	1 cut(s)	1180
BbrPI	CACGTG	1 cut(s)	271
BbsI	GAAGAC	3 cut(s)	732, 837, 1306
Bbv12I	GWGCWC	2 cut(s)	174, 1180
BbvI	GCAGC	4 cut(s)	268, 450, 693, 914
BccI	CCATC	4 cut(s)	149, 754, 982, 1374
BcgI	CGANNNNNNTGC	2 cut(s)	1514, 1548
BciT130I	CCWGG	2 cut(s)	176, 290
BciVI	GTATCC	1 cut(s)	1503
BclI	TGATCA	1 cut(s)	868
BcnI	CCSGG	5 cut(s)	242, 243, 1583, 1653, 1654
BcoDI	GTCTC	3 cut(s)	693, 953, 1642
BfaI	CTAG	2 cut(s)	69, 998
BfoI	RGCGCY	1 cut(s)	935
BfuI	GTATCC	1 cut(s)	1503
BglI	GCCNNNNNGGC	1 cut(s)	243
BisI	GCNGC	7 cut(s)	86, 252, 257, 439, 707, 903, 1056
BlsI	GCNGC	7 cut(s)	87, 253, 258, 440, 708, 904, 1057
Bme1390I	CCNGG	7 cut(s)	176, 242, 243, 290, 1583, 1653, 1654
Bme18I	GGWCC	5 cut(s)	130, 156, 543, 1298, 1596
BmeRI	GACNNNNNGTC	1 cut(s)	136
BmeT110I	CYCGRG	3 cut(s)	241, 1553, 1652
BmgBI	CACGTC	1 cut(s)	1626
BmgT120I	GGNCC	7 cut(s)	80, 130, 156, 187, 543, 1298, 1596
BmiI	GGNNCC	2 cut(s)	131, 1299
BmrFI	CCNGG	7 cut(s)	176, 242, 243, 290, 1583, 1653, 1654
BmrI	ACTGGG	1 cut(s)	423
BmsI	GCATC	3 cut(s)	982, 1158, 1218
BmtI	GCTAGC	1 cut(s)	72
BmuI	ACTGGG	1 cut(s)	423
BpiI	GAAGAC	3 cut(s)	732, 837, 1306
BpmI	CTGGAG	2 cut(s)	206, 272
Bpu10I	CCTNAGC	1 cut(s)	120
Bpu14I	TTCGAA	1 cut(s)	821
BpuMI	CCSGG	5 cut(s)	242, 243, 1583, 1653, 1654
BsaAI	YACGTR	1 cut(s)	271
BsaBI	GATNNNNATC	1 cut(s)	140
BsaHI	GRCGYC	3 cut(s)	93, 204, 1021
BsaJI	CCNNGG	3 cut(s)	175, 241, 1652
BsaWI	WCCGGW	6 cut(s)	54, 132, 153, 367, 715, 1569
BsaXI	ACNNNNNCTCC	2 cut(s)	270, 300
Bsc4I	CCNNNNNNNGG	6 cut(s)	241, 247, 1160, 1254, 1569, 1659
Bse118I	RCCGGY	1 cut(s)	77
Bse1I	ACTGG	3 cut(s)	148, 223, 418
Bse3DI	GCAATG	2 cut(s)	19, 461
Bse8I	GATNNNNATC	1 cut(s)	140
BseAI	TCCGGA	2 cut(s)	153, 715
BseBI	CCWGG	2 cut(s)	176, 290
BseDI	CCNNGG	3 cut(s)	175, 241, 1652
BseGI	GGATG	6 cut(s)	141, 997, 1173, 1242, 1385, 1507
BseJI	GATNNNNATC	1 cut(s)	140
BseLI	CCNNNNNNNGG	6 cut(s)	241, 247, 1160, 1254, 1569, 1659
BseMI	GCAATG	2 cut(s)	19, 461
BseMII	CTCAG	3 cut(s)	134, 416, 927
BseNI	ACTGG	3 cut(s)	148, 223, 418
BseRI	GAGGAG	2 cut(s)	209, 1129
BseXI	GCAGC	4 cut(s)	268, 450, 693, 914
Bsh1236I	CGCG	1 cut(s)	254
BshFI	GGCC	5 cut(s)	77, 81, 188, 251, 502
BsiHKAI	GWGCWC	2 cut(s)	174, 1180
BsiHKCI	CYCGRG	3 cut(s)	241, 1553, 1652
BslFI	GGGAC	6 cut(s)	20, 143, 291, 431, 1535, 1652
BslI	CCNNNNNNNGG	6 cut(s)	241, 247, 1160, 1254, 1569, 1659
BsmAI	GTCTC	3 cut(s)	693, 953, 1642
BsmBI	CGTCTC	2 cut(s)	953, 1642
BsmFI	GGGAC	6 cut(s)	20, 143, 291, 431, 1535, 1652
BsmI	GAATGC	2 cut(s)	367, 1535
BsnI	GGCC	5 cut(s)	77, 81, 188, 251, 502
BsoBI	CYCGRG	3 cut(s)	241, 1553, 1652
Bsp119I	TTCGAA	1 cut(s)	821
Bsp1286I	GDGCHC	2 cut(s)	174, 1180
Bsp13I	TCCGGA	2 cut(s)	153, 715
Bsp143I	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
BspANI	GGCC	5 cut(s)	77, 81, 188, 251, 502
BspCNI	CTCAG	3 cut(s)	133, 417, 928
BspEI	TCCGGA	2 cut(s)	153, 715
BspFNI	CGCG	1 cut(s)	254
BspLI	GGNNCC	2 cut(s)	131, 1299
BspOI	GCTAGC	1 cut(s)	72
BspPI	GGATC	2 cut(s)	1313, 1664
BspT104I	TTCGAA	1 cut(s)	821
BsrBI	CCGCTC	1 cut(s)	554
BsrDI	GCAATG	2 cut(s)	19, 461
BsrFI	RCCGGY	1 cut(s)	77
BsrI	ACTGG	3 cut(s)	148, 223, 418
BssAI	RCCGGY	1 cut(s)	77
BssECI	CCNNGG	3 cut(s)	175, 241, 1652
BssMI	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
BssNI	GRCGYC	3 cut(s)	93, 204, 1021
Bst2UI	CCWGG	2 cut(s)	176, 290
Bst4CI	ACNGT	2 cut(s)	893, 1549
Bst6I	CTCTTC	4 cut(s)	312, 849, 873, 1521
BstACI	GRCGYC	3 cut(s)	93, 204, 1021
BstBAI	YACGTR	1 cut(s)	271
BstBI	TTCGAA	1 cut(s)	821
BstC8I	GCNNGC	6 cut(s)	70, 79, 83, 1464, 1560, 1619
BstDEI	CTNAG	5 cut(s)	120, 425, 936, 1025, 1106
BstF5I	GGATG	6 cut(s)	141, 997, 1173, 1242, 1385, 1507
BstFNI	CGCG	1 cut(s)	254
BstH2I	RGCGCY	1 cut(s)	935
BstHHI	GCGC	2 cut(s)	256, 934
BstKTI	GATC	6 cut(s)	496, 643, 871, 1066, 1321, 1659
BstMAI	GTCTC	3 cut(s)	693, 953, 1642
BstMBI	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
BstMWI	GCNNNNNNNGC	9 cut(s)	74, 78, 107, 116, 243, 265, 671, 902, 908
BstNI	CCWGG	2 cut(s)	176, 290
BstNSI	RCATGY	2 cut(s)	599, 1160
BstSCI	CCNGG	7 cut(s)	174, 240, 241, 288, 1581, 1651, 1652
BstUI	CGCG	1 cut(s)	254
BstV1I	GCAGC	4 cut(s)	268, 450, 693, 914
BstV2I	GAAGAC	3 cut(s)	732, 837, 1306
BsuI	GTATCC	1 cut(s)	1503
BsuRI	GGCC	5 cut(s)	77, 81, 188, 251, 502
BtgZI	GCGATG	1 cut(s)	456
BtrI	CACGTC	1 cut(s)	1626
BtsCI	GGATG	6 cut(s)	141, 997, 1173, 1242, 1385, 1507
BtsI	GCAGTG	1 cut(s)	871
BtsIMutI	CAGTG	2 cut(s)	871, 898
Cac8I	GCNNGC	6 cut(s)	70, 79, 83, 1464, 1560, 1619
CaiI	CAGNNNCTG	1 cut(s)	935
CfoI	GCGC	2 cut(s)	256, 934
Cfr10I	RCCGGY	1 cut(s)	77
Cfr13I	GGNCC	7 cut(s)	80, 130, 156, 187, 543, 1298, 1596
Cfr9I	CCCGGG	2 cut(s)	241, 1652
CseI	GACGC	4 cut(s)	212, 571, 935, 1411
Csp6I	GTAC	5 cut(s)	115, 347, 940, 1114, 1216
CspCI	CAANNNNNGTGG	2 cut(s)	817, 852
CviAII	CATG	8 cut(s)	353, 536, 596, 1067, 1157, 1172, 1232, 1369
CviQI	GTAC	5 cut(s)	115, 347, 940, 1114, 1216
DdeI	CTNAG	5 cut(s)	120, 425, 936, 1025, 1106
DpnI	GATC	6 cut(s)	495, 642, 870, 1065, 1320, 1658
DpnII	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
DraI	TTTAAA	2 cut(s)	762, 1243
DriI	GACNNNNNGTC	1 cut(s)	136
EaeI	YGGCCR	1 cut(s)	500
Eam1104I	CTCTTC	4 cut(s)	312, 849, 873, 1521
Eam1105I	GACNNNNNGTC	1 cut(s)	136
EarI	CTCTTC	4 cut(s)	312, 849, 873, 1521
Ecl136II	GAGCTC	1 cut(s)	1178
Eco24I	GRGCYC	1 cut(s)	1180
Eco47I	GGWCC	5 cut(s)	130, 156, 543, 1298, 1596
Eco53kI	GAGCTC	1 cut(s)	1178
Eco57I	CTGAAG	4 cut(s)	644, 873, 927, 1080
Eco72I	CACGTG	1 cut(s)	271
Eco88I	CYCGRG	3 cut(s)	241, 1553, 1652
EcoICRI	GAGCTC	1 cut(s)	1178
EcoO109I	RGGNCCY	1 cut(s)	543
EcoRI	GAATTC	1 cut(s)	740
EcoRII	CCWGG	2 cut(s)	174, 288
EcoT22I	ATGCAT	2 cut(s)	1173, 1233
EcoT38I	GRGCYC	1 cut(s)	1180
Esp3I	CGTCTC	2 cut(s)	953, 1642
FaeI	CATG	8 cut(s)	356, 539, 599, 1070, 1160, 1175, 1235, 1372
FalI	AAGNNNNNCTT	2 cut(s)	1265, 1297
FaqI	GGGAC	6 cut(s)	20, 143, 291, 431, 1535, 1652
FatI	CATG	8 cut(s)	352, 535, 595, 1066, 1156, 1171, 1231, 1368
FauI	CCCGC	4 cut(s)	90, 119, 242, 1610
FbaI	TGATCA	1 cut(s)	868
Fnu4HI	GCNGC	7 cut(s)	86, 252, 257, 439, 707, 903, 1056
FokI	GGATG	6 cut(s)	148, 1004, 1180, 1249, 1392, 1514
FriOI	GRGCYC	1 cut(s)	1180
FseI	GGCCGGCC	1 cut(s)	81
Fsp4HI	GCNGC	7 cut(s)	86, 252, 257, 439, 707, 903, 1056
FspBI	CTAG	2 cut(s)	69, 998
GlaI	GCGC	2 cut(s)	255, 933
GluI	GCNGC	7 cut(s)	86, 252, 257, 439, 707, 903, 1056
GsuI	CTGGAG	2 cut(s)	206, 272
HaeII	RGCGCY	1 cut(s)	935
HaeIII	GGCC	5 cut(s)	77, 81, 188, 251, 502
HgaI	GACGC	4 cut(s)	212, 571, 935, 1411
HhaI	GCGC	2 cut(s)	256, 934
Hin1I	GRCGYC	3 cut(s)	93, 204, 1021
Hin1II	CATG	8 cut(s)	356, 539, 599, 1070, 1160, 1175, 1235, 1372
Hin6I	GCGC	2 cut(s)	254, 932
HinP1I	GCGC	2 cut(s)	254, 932
HindIII	AAGCTT	1 cut(s)	656
HinfI	GANTC	7 cut(s)	182, 279, 418, 755, 809, 1131, 1412
HphI	GGTGA	7 cut(s)	603, 635, 741, 992, 1019, 1319, 1369
Hpy166II	GTNNAC	5 cut(s)	228, 349, 1037, 1089, 1114
Hpy188I	TCNGA	6 cut(s)	202, 338, 853, 1302, 1600, 1642
Hpy188III	TCNNGA	5 cut(s)	154, 229, 716, 872, 1553
Hpy8I	GTNNAC	5 cut(s)	228, 349, 1037, 1089, 1114
Hpy99I	CGWCG	2 cut(s)	95, 206
HpyAV	CCTTC	6 cut(s)	534, 817, 834, 1348, 1426, 1678
HpyCH4III	ACNGT	2 cut(s)	893, 1549
HpyCH4IV	ACGT	4 cut(s)	93, 270, 1021, 1625
HpyF10VI	GCNNNNNNNGC	9 cut(s)	74, 78, 107, 116, 243, 265, 671, 902, 908
HpyF3I	CTNAG	5 cut(s)	120, 425, 936, 1025, 1106
HpySE526I	ACGT	4 cut(s)	93, 270, 1021, 1625
Hsp92I	GRCGYC	3 cut(s)	93, 204, 1021
Hsp92II	CATG	8 cut(s)	356, 539, 599, 1070, 1160, 1175, 1235, 1372
HspAI	GCGC	2 cut(s)	254, 932
Kpn2I	TCCGGA	2 cut(s)	153, 715
KroI	GCCGGC	1 cut(s)	77
KroNI	GCCGGC	1 cut(s)	79
Ksp22I	TGATCA	1 cut(s)	868
Kzo9I	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
LmnI	GCTCC	3 cut(s)	370, 559, 1175
Lsp1109I	GCAGC	4 cut(s)	268, 450, 693, 914
LweI	GCATC	3 cut(s)	982, 1158, 1218
MaeI	CTAG	2 cut(s)	69, 998
MaeII	ACGT	4 cut(s)	93, 270, 1021, 1625
MaeIII	GTNAC	2 cut(s)	559, 1262
MalI	GATC	6 cut(s)	495, 642, 870, 1065, 1320, 1658
MbiI	CCGCTC	1 cut(s)	554
MboI	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
MfeI	CAATTG	1 cut(s)	1219
MhlI	GDGCHC	2 cut(s)	174, 1180
MlsI	TGGCCA	1 cut(s)	502
MluNI	TGGCCA	1 cut(s)	502
MlyI	GAGTC	2 cut(s)	191, 273
MmeI	TCCRAC	2 cut(s)	225, 1435
Mox20I	TGGCCA	1 cut(s)	502
Mph1103I	ATGCAT	2 cut(s)	1173, 1233
MroI	TCCGGA	2 cut(s)	153, 715
MroNI	GCCGGC	1 cut(s)	77
MroXI	GAANNNNTTC	1 cut(s)	844
MscI	TGGCCA	1 cut(s)	502
MseI	TTAA	6 cut(s)	477, 761, 986, 1194, 1242, 1268
Msp20I	TGGCCA	1 cut(s)	502
MspA1I	CMGCKG	1 cut(s)	905
MspR9I	CCNGG	7 cut(s)	176, 242, 243, 290, 1583, 1653, 1654
MunI	CAATTG	1 cut(s)	1219
Mva1269I	GAATGC	2 cut(s)	367, 1535
MvaI	CCWGG	2 cut(s)	176, 290
MvnI	CGCG	1 cut(s)	254
MwoI	GCNNNNNNNGC	9 cut(s)	74, 78, 107, 116, 243, 265, 671, 902, 908
NaeI	GCCGGC	1 cut(s)	79
NciI	CCSGG	5 cut(s)	242, 243, 1583, 1653, 1654
NdeII	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
NgoMIV	GCCGGC	1 cut(s)	77
NheI	GCTAGC	1 cut(s)	68
NlaIII	CATG	8 cut(s)	356, 539, 599, 1070, 1160, 1175, 1235, 1372
NlaIV	GGNNCC	2 cut(s)	131, 1299
NmuCI	GTSAC	1 cut(s)	559
NsiI	ATGCAT	2 cut(s)	1173, 1233
NspI	RCATGY	2 cut(s)	599, 1160
NspV	TTCGAA	1 cut(s)	821
PciI	ACATGT	1 cut(s)	1156
PctI	GAATGC	2 cut(s)	367, 1535
PdiI	GCCGGC	1 cut(s)	79
PdmI	GAANNNNTTC	1 cut(s)	844
PfeI	GAWTC	5 cut(s)	418, 755, 809, 1131, 1412
PflMI	CCANNNNNTGG	1 cut(s)	1160
PfoI	TCCNGGA	2 cut(s)	288, 1581
PkrI	GCNGC	7 cut(s)	87, 253, 258, 440, 708, 904, 1057
PleI	GAGTC	2 cut(s)	190, 273
PmaCI	CACGTG	1 cut(s)	271
PmlI	CACGTG	1 cut(s)	271
PpsI	GAGTC	2 cut(s)	190, 273
Ppu21I	YACGTR	1 cut(s)	271
PpuMI	RGGWCCY	1 cut(s)	543
PscI	ACATGT	1 cut(s)	1156
PsiI	TTATAA	1 cut(s)	629
Psp124BI	GAGCTC	1 cut(s)	1180
Psp5II	RGGWCCY	1 cut(s)	543
Psp6I	CCWGG	2 cut(s)	174, 288
PspCI	CACGTG	1 cut(s)	271
PspGI	CCWGG	2 cut(s)	174, 288
PspN4I	GGNNCC	2 cut(s)	131, 1299
PspPI	GGNCC	7 cut(s)	80, 130, 156, 187, 543, 1298, 1596
PspPPI	RGGWCCY	1 cut(s)	543
PstNI	CAGNNNCTG	1 cut(s)	935
PvuII	CAGCTG	1 cut(s)	905
RigI	GGCCGGCC	1 cut(s)	81
RsaI	GTAC	5 cut(s)	116, 348, 941, 1115, 1217
RsaNI	GTAC	5 cut(s)	115, 347, 940, 1114, 1216
SacI	GAGCTC	1 cut(s)	1180
SaqAI	TTAA	6 cut(s)	477, 761, 986, 1194, 1242, 1268
SatI	GCNGC	7 cut(s)	86, 252, 257, 439, 707, 903, 1056
Sau3AI	GATC	6 cut(s)	493, 640, 868, 1063, 1318, 1656
Sau96I	GGNCC	7 cut(s)	80, 130, 156, 187, 543, 1298, 1596
SchI	GAGTC	2 cut(s)	191, 273
ScrFI	CCNGG	7 cut(s)	176, 242, 243, 290, 1583, 1653, 1654
SduI	GDGCHC	2 cut(s)	174, 1180
SfaNI	GCATC	3 cut(s)	982, 1158, 1218
SfuI	TTCGAA	1 cut(s)	821
SinI	GGWCC	5 cut(s)	130, 156, 543, 1298, 1596
SmaI	CCCGGG	2 cut(s)	243, 1654
SspMI	CTAG	2 cut(s)	69, 998
SstI	GAGCTC	1 cut(s)	1180
StyD4I	CCNGG	7 cut(s)	174, 240, 241, 288, 1581, 1651, 1652
TaaI	ACNGT	2 cut(s)	893, 1549
TaiI	ACGT	4 cut(s)	96, 273, 1024, 1628
TaqI	TCGA	5 cut(s)	144, 168, 584, 821, 1317
TatI	WGTACW	2 cut(s)	939, 1215
TauI	GCSGC	3 cut(s)	88, 254, 1058
TfiI	GAWTC	5 cut(s)	418, 755, 809, 1131, 1412
Tru1I	TTAA	6 cut(s)	477, 761, 986, 1194, 1242, 1268
Tru9I	TTAA	6 cut(s)	477, 761, 986, 1194, 1242, 1268
TscAI	CASTG	2 cut(s)	871, 898
TseFI	GTSAC	1 cut(s)	559
TseI	GCWGC	4 cut(s)	256, 438, 706, 902
Tsp45I	GTSAC	1 cut(s)	559
TspDTI	ATGAA	6 cut(s)	290, 373, 374, 837, 891, 906
TspGWI	ACGGA	1 cut(s)	1266
TspMI	CCCGGG	2 cut(s)	241, 1652
TspRI	CASTG	2 cut(s)	871, 898
Van91I	CCANNNNNTGG	1 cut(s)	1160
VpaK11BI	GGWCC	5 cut(s)	130, 156, 543, 1298, 1596
XapI	RAATTY	2 cut(s)	740, 1478
XceI	RCATGY	2 cut(s)	599, 1160
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1161
XmaI	CCCGGG	2 cut(s)	241, 1652
XmnI	GAANNNNTTC	1 cut(s)	844
XspI	CTAG	2 cut(s)	69, 998
ZraI	GACGTC	2 cut(s)	94, 1022
Zsp2I	ATGCAT	2 cut(s)	1173, 1233

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.