Rmu_sc0041308.1_g000001

Phosphoinositide phospholipase C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0041308.1
OG0008969
Physical Location & Seq
Reverse (-)
2 .. 1322
1321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0041308.1_g000001.1.cds

Sequence Viewer

Length: 885 bp
atgtcccattctcatggatcatcatcggaatatccaaaatccaaaaccaaagagagtaagactttcggctcagcatcggagaaaaagatcaagaaaatgggcgacggaaataacaccaacaactacaagatgttcaagttcttcaaccgcaagttcaagatcagcgagttgaagccacctcccgacgtcaaggacatcttctccaagttcgccgccggcggggaactcatatcccccgatcagttccatacgttcttgatcgaacaccaggccgagaaatccgtttctttggccgatgccaagcgcatcctacatgagttctccctccgacgacagcatcttacggacccgaaacacaagaaagacaagaaacagtccaaagagaacaaaactgccgcgccggcccaccaaactcatgagcacggcctcactttggacgaattcttcaatttcctattcgaggaagaattcaacggccccatcaaacgagaggtacaccatgatatgactgctccgttgtcgcattatttcatatatacagggcacaattcgtaccttactgggaatcaactcagcagtgactgtagtgatgtgccaatcattaaggctttggagagaggggtcaaagtaattgagcttgatttatggccaacgtcttcgaaacaggatgttcaagtttatcatggaaggaccttgacggctcctgtgacactcgtcaaatgcttgaaatccattaaagaacatgcttttgataaatctccataccctgttgttattacttttgaagaccaccttacgaccaagcttcggtgtattgttgcagagatggttacccaaatatttggagatatgttgtattatccaaagccagaagacccgatgaca
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00562 ko01100 ko04020 ko04070 ko04919 ko04933 map00562 map01100 map04020 map04070 map04919 map04933
Pfam Domains
Protein Families
C2 EF-hand_like PI-PLC-X PI-PLC-Y

Protein Analysis

295

Amino Acids

33.76

Weight (kDa)

8.7

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
AccII CGCG 1 cut(s) 398
AciI CCGC 4 cut(s) 148, 213, 219, 396
AclWI GGATC 1 cut(s) 25
AcoI YGGCCR 2 cut(s) 291, 647
AcsI RAATTY 2 cut(s) 440, 467
AcyI GRCGYC 1 cut(s) 186
AfaI GTAC 2 cut(s) 495, 554
AfiI CCNNNNNNNGG 4 cut(s) 219, 433, 460, 807
AgsI TTSAA 9 cut(s) 136, 145, 157, 172, 448, 472, 674, 727, 785
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 2 cut(s) 637, 805
AluI AGCT 2 cut(s) 637, 805
Alw21I GWGCWC 1 cut(s) 423
AlwI GGATC 1 cut(s) 25
AlwNI CAGNNNCTG 1 cut(s) 582
AoxI GGCC 6 cut(s) 270, 291, 402, 424, 475, 647
ApoI RAATTY 2 cut(s) 440, 467
AspLEI GCGC 2 cut(s) 306, 400
AspS9I GGNCC 4 cut(s) 346, 403, 476, 690
AsuII TTCGAA 1 cut(s) 659
AvaII GGWCC 2 cut(s) 346, 690
BaeGI GKGCMC 1 cut(s) 546
BalI TGGCCA 1 cut(s) 649
BbsI GAAGAC 3 cut(s) 648, 792, 879
Bbv12I GWGCWC 1 cut(s) 423
BccI CCATC 2 cut(s) 488, 820
BceAI ACGGC 3 cut(s) 439, 490, 714
BciT130I CCWGG 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 583
BglI GCCNNNNNGGC 1 cut(s) 401
BisI GCNGC 2 cut(s) 213, 396
BlpI GCTNAGC 1 cut(s) 70
BlsI GCNGC 2 cut(s) 214, 397
Bme1390I CCNGG 1 cut(s) 269
Bme18I GGWCC 2 cut(s) 346, 690
BmgT120I GGNCC 4 cut(s) 346, 403, 476, 690
BmiI GGNNCC 3 cut(s) 348, 478, 702
BmrFI CCNGG 1 cut(s) 269
BmrI ACTGGG 1 cut(s) 570
BmsI GCATC 4 cut(s) 83, 286, 315, 346
BmuI ACTGGG 1 cut(s) 570
BoxI GACNNNNGTC 1 cut(s) 713
BpiI GAAGAC 3 cut(s) 648, 792, 879
Bpu1102I GCTNAGC 1 cut(s) 70
Bpu14I TTCGAA 1 cut(s) 659
BsaBI GATNNNNATC 1 cut(s) 22
BsaHI GRCGYC 1 cut(s) 186
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 4 cut(s) 219, 433, 460, 807
Bse118I RCCGGY 2 cut(s) 215, 400
Bse1I ACTGG 1 cut(s) 565
Bse8I GATNNNNATC 1 cut(s) 22
BseBI CCWGG 1 cut(s) 269
BseGI GGATG 2 cut(s) 306, 673
BseJI GATNNNNATC 1 cut(s) 22
BseLI CCNNNNNNNGG 4 cut(s) 219, 433, 460, 807
BseMII CTCAG 2 cut(s) 84, 586
BseNI ACTGG 1 cut(s) 565
BseSI GKGCMC 1 cut(s) 546
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 6 cut(s) 272, 293, 404, 426, 477, 649
BsiHKAI GWGCWC 1 cut(s) 423
BsiSI CCGG 2 cut(s) 216, 401
BslI CCNNNNNNNGG 4 cut(s) 219, 433, 460, 807
BsnI GGCC 6 cut(s) 272, 293, 404, 426, 477, 649
Bsp119I TTCGAA 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 423, 546
Bsp143I GATC 5 cut(s) 17, 87, 159, 238, 258
Bsp1720I GCTNAGC 1 cut(s) 70
BspACI CCGC 4 cut(s) 148, 213, 219, 396
BspANI GGCC 6 cut(s) 272, 293, 404, 426, 477, 649
BspCNI CTCAG 2 cut(s) 83, 585
BspFNI CGCG 1 cut(s) 398
BspHI TCATGA 1 cut(s) 415
BspLI GGNNCC 3 cut(s) 348, 478, 702
BspPI GGATC 1 cut(s) 25
BspT104I TTCGAA 1 cut(s) 659
BsrFI RCCGGY 2 cut(s) 215, 400
BsrI ACTGG 1 cut(s) 565
BssAI RCCGGY 2 cut(s) 215, 400
BssMI GATC 5 cut(s) 17, 87, 159, 238, 258
BssNI GRCGYC 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 375, 584
BstACI GRCGYC 1 cut(s) 186
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 2 cut(s) 217, 402
BstDEI CTNAG 2 cut(s) 70, 572
BstEII GGTNACC 1 cut(s) 829
BstENI CCTNNNNNAGG 1 cut(s) 458
BstF5I GGATG 2 cut(s) 306, 673
BstFNI CGCG 1 cut(s) 398
BstHHI GCGC 2 cut(s) 306, 400
BstKTI GATC 5 cut(s) 20, 90, 162, 241, 261
BstMBI GATC 5 cut(s) 17, 87, 159, 238, 258
BstMWI GCNNNNNNNGC 1 cut(s) 401
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 1 cut(s) 746
BstPAI GACNNNNGTC 1 cut(s) 713
BstPI GGTNACC 1 cut(s) 829
BstSCI CCNGG 1 cut(s) 267
BstSFI CTRYAG 1 cut(s) 583
BstSLI GKGCMC 1 cut(s) 546
BstUI CGCG 1 cut(s) 398
BstV2I GAAGAC 3 cut(s) 648, 792, 879
BstXI CCANNNNNNTGG 2 cut(s) 14, 842
BsuRI GGCC 6 cut(s) 272, 293, 404, 426, 477, 649
BtsCI GGATG 2 cut(s) 306, 673
BtsI GCAGTG 1 cut(s) 583
BtsIMutI CAGTG 1 cut(s) 583
Cac8I GCNNGC 2 cut(s) 217, 402
CaiI CAGNNNCTG 1 cut(s) 582
CciI TCATGA 1 cut(s) 415
CfoI GCGC 2 cut(s) 306, 400
Cfr10I RCCGGY 2 cut(s) 215, 400
Cfr13I GGNCC 4 cut(s) 346, 403, 476, 690
Csp6I GTAC 2 cut(s) 494, 553
CviAII CATG 6 cut(s) 14, 314, 416, 500, 683, 743
CviQI GTAC 2 cut(s) 494, 553
DdeI CTNAG 2 cut(s) 70, 572
DpnI GATC 5 cut(s) 19, 89, 161, 240, 260
DpnII GATC 5 cut(s) 17, 87, 159, 238, 258
EaeI YGGCCR 2 cut(s) 291, 647
Eco47I GGWCC 2 cut(s) 346, 690
Eco91I GGTNACC 1 cut(s) 829
EcoNI CCTNNNNNAGG 1 cut(s) 458
EcoO109I RGGNCCY 1 cut(s) 690
EcoO65I GGTNACC 1 cut(s) 829
EcoRI GAATTC 2 cut(s) 440, 467
EcoRII CCWGG 1 cut(s) 267
FaeI CATG 6 cut(s) 17, 317, 419, 503, 686, 746
FalI AAGNNNNNCTT 4 cut(s) 182, 214, 777, 809
FatI CATG 6 cut(s) 13, 313, 415, 499, 682, 742
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 2 cut(s) 213, 396
FokI GGATG 2 cut(s) 293, 680
Fsp4HI GCNGC 2 cut(s) 213, 396
GlaI GCGC 2 cut(s) 305, 399
GluI GCNGC 2 cut(s) 213, 396
HaeIII GGCC 6 cut(s) 272, 293, 404, 426, 477, 649
HapII CCGG 2 cut(s) 216, 401
HhaI GCGC 2 cut(s) 306, 400
Hin1I GRCGYC 1 cut(s) 186
Hin1II CATG 6 cut(s) 17, 317, 419, 503, 686, 746
Hin6I GCGC 2 cut(s) 304, 398
HinP1I GCGC 2 cut(s) 304, 398
HindIII AAGCTT 1 cut(s) 803
HinfI GANTC 1 cut(s) 565
HpaII CCGG 2 cut(s) 216, 401
Hpy166II GTNNAC 1 cut(s) 496
Hpy188I TCNGA 3 cut(s) 28, 79, 329
Hpy188III TCNNGA 5 cut(s) 91, 157, 182, 256, 416
Hpy8I GTNNAC 1 cut(s) 496
Hpy99I CGWCG 3 cut(s) 107, 188, 333
HpyAV CCTTC 1 cut(s) 681
HpyCH4III ACNGT 2 cut(s) 375, 584
HpyCH4IV ACGT 3 cut(s) 186, 251, 653
HpyCH4V TGCA 1 cut(s) 821
HpyF10VI GCNNNNNNNGC 1 cut(s) 401
HpyF3I CTNAG 2 cut(s) 70, 572
HpySE526I ACGT 3 cut(s) 186, 251, 653
Hsp92I GRCGYC 1 cut(s) 186
Hsp92II CATG 6 cut(s) 17, 317, 419, 503, 686, 746
HspAI GCGC 2 cut(s) 304, 398
KroI GCCGGC 2 cut(s) 215, 400
KroNI GCCGGC 2 cut(s) 217, 402
Kzo9I GATC 5 cut(s) 17, 87, 159, 238, 258
LmnI GCTCC 2 cut(s) 517, 706
LpnPI CCDG 9 cut(s) 229, 254, 281, 414, 525, 546, 650, 717, 780
LweI GCATC 4 cut(s) 83, 286, 315, 346
MaeII ACGT 3 cut(s) 186, 251, 653
MaeIII GTNAC 3 cut(s) 578, 706, 829
MalI GATC 5 cut(s) 19, 89, 161, 240, 260
MboI GATC 5 cut(s) 17, 87, 159, 238, 258
MboII GAAGA 7 cut(s) 133, 190, 436, 476, 648, 797, 884
MhlI GDGCHC 2 cut(s) 423, 546
MlsI TGGCCA 1 cut(s) 649
MluCI AATT 5 cut(s) 440, 448, 467, 547, 630
MluNI TGGCCA 1 cut(s) 649
MmeI TCCRAC 1 cut(s) 352
MnlI CCTC 6 cut(s) 189, 335, 437, 454, 484, 611
Mox20I TGGCCA 1 cut(s) 649
MreI CGCCGGCG 1 cut(s) 215
MroNI GCCGGC 2 cut(s) 215, 400
MscI TGGCCA 1 cut(s) 649
MseI TTAA 2 cut(s) 603, 735
MslI CAYNNNNRTG 1 cut(s) 12
Msp20I TGGCCA 1 cut(s) 649
MspI CCGG 2 cut(s) 216, 401
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 1 cut(s) 401
NaeI GCCGGC 2 cut(s) 217, 402
NdeII GATC 5 cut(s) 17, 87, 159, 238, 258
NgoMIV GCCGGC 2 cut(s) 215, 400
NlaIII CATG 6 cut(s) 17, 317, 419, 503, 686, 746
NlaIV GGNNCC 3 cut(s) 348, 478, 702
NmeAIII GCCGAG 1 cut(s) 298
NmuCI GTSAC 2 cut(s) 578, 706
NspI RCATGY 1 cut(s) 746
NspV TTCGAA 1 cut(s) 659
PagI TCATGA 1 cut(s) 415
PdiI GCCGGC 2 cut(s) 217, 402
PfeI GAWTC 1 cut(s) 565
PkrI GCNGC 2 cut(s) 214, 397
PpuMI RGGWCCY 1 cut(s) 690
PshAI GACNNNNGTC 1 cut(s) 713
Psp5II RGGWCCY 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 267
PspEI GGTNACC 1 cut(s) 829
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 3 cut(s) 348, 478, 702
PspPI GGNCC 4 cut(s) 346, 403, 476, 690
PspPPI RGGWCCY 1 cut(s) 690
PstNI CAGNNNCTG 1 cut(s) 582
RsaI GTAC 2 cut(s) 495, 554
RsaNI GTAC 2 cut(s) 494, 553
RseI CAYNNNNRTG 1 cut(s) 12
SaqAI TTAA 2 cut(s) 603, 735
SatI GCNGC 2 cut(s) 213, 396
Sau3AI GATC 5 cut(s) 17, 87, 159, 238, 258
Sau96I GGNCC 4 cut(s) 346, 403, 476, 690
ScrFI CCNGG 1 cut(s) 269
SduI GDGCHC 2 cut(s) 423, 546
SfaNI GCATC 4 cut(s) 83, 286, 315, 346
SfcI CTRYAG 1 cut(s) 583
SfuI TTCGAA 1 cut(s) 659
SgrAI CRCCGGYG 1 cut(s) 215
SinI GGWCC 2 cut(s) 346, 690
SmiMI CAYNNNNRTG 1 cut(s) 12
Sse9I AATT 5 cut(s) 440, 448, 467, 547, 630
SsiI CCGC 4 cut(s) 148, 213, 219, 396
SspI AATATT 1 cut(s) 840
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 375, 584
TaiI ACGT 3 cut(s) 189, 254, 656
TaqI TCGA 3 cut(s) 261, 459, 659
TasI AATT 5 cut(s) 440, 448, 467, 547, 630
TauI GCSGC 2 cut(s) 215, 398
TfiI GAWTC 1 cut(s) 565
Tru1I TTAA 2 cut(s) 603, 735
Tru9I TTAA 2 cut(s) 603, 735
TscAI CASTG 1 cut(s) 583
TseFI GTSAC 2 cut(s) 578, 706
Tsp45I GTSAC 2 cut(s) 578, 706
TspDTI ATGAA 1 cut(s) 520
TspGWI ACGGA 4 cut(s) 120, 271, 359, 504
TspRI CASTG 1 cut(s) 583
VpaK11BI GGWCC 2 cut(s) 346, 690
XagI CCTNNNNNAGG 1 cut(s) 458
XapI RAATTY 2 cut(s) 440, 467
XceI RCATGY 1 cut(s) 746
ZraI GACGTC 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.