AT2G40480

WEB family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0008639
Physical Location & Seq
Forward (+)
16910167 .. 16912616
2450 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G40480.1

Sequence Viewer

Length: 1557 bp
ATGGCATTGGCAGAGGAGCAGCATCAGAATTATCATGTGGCTGAGGCGATTCCGGGTACGCCTGGGATCCATGATGTCAGGATTCAACCCGGTTCTGAGAATTCCGGGTTCTGTGTTGATCCGGTTGGTGTTTCTAATGTACCTGGAATTAGGAGAGTTGGGTTGAGAGCTGAAATCGATACTTCTCCTCCATTCGGATCGGTTCAGGAGGCGGTGACTCGATTCGGTGGTCGTGGTTATTGGGTTCCTTTCAAGCTTGATGACACTTTCAATGGTGAATTTGACATTAAGAGAATGGAGGAGCATGCAGCGGAGCTTGAGAAGGATCTGATTGTGAAAGAGCTTGAAACTTTGGATGTTTTAGAAGCTCTTGGATCAACCAAAAGGATCGTTGAAGATTTGAAGCGGCAGCTTCAGCAAGAAGCACTGAGATGCTCTGACCAACTCAGTTCAGATATCAAAGAGATGAACGATGAGCATTGCCATCACAATCCCATGTCATCTCCTGATTTGATCTTGATGGAATTGAAGCAAGCTAAGATGAATCTTGGTAAAACCATGGACAACCTTGTAGTGATCCAGTCCTCTGTTGAATCTCTGAATAAGAAAATGAAAGAAGAGAAAGACTTCCTTGAGAAGACACGGGCTAAGCTAACGTATGGGTTTGGTGGACCGGTATCTCTAGCTGAGGAGTTGAGTCGGATTAAGGTAAAACCACAGGTTCAAGATGAACCGTTAAGGGAGCAGGTTAAGATGGTTGCTGAAGCCGATGAAACGGGTTTAAACCTGCAGAACAAGAACAGTCTGAGAACTGCAGAGATGAGATTGGTTGCTGCTAGGAAAATGGAAGAAGCTGCGAAAGCAGCGGAAGCACTTGCGATTGCTGAAATTACAATGTTATCATCCAATGGTGAAAGCCAGGACGATGATTCGGAATTTTGCTTTCCAGAGCCTCCAAGATCTCCTGTGACACCAAGAGGACTCAGGATTGATAATGACTTCTCCACGGATAAGAGTTCAAGACGAGGGATTTTGAAGAAGCTTGAGGAAGCTACTGAAGGAGTTAAACAAAGTAAGCAGGCTTTAGAAGCTGCGTTAAACCGAGTAGAGATCGCTAATGTGAAGCAACTTGCAGCTGAAAATGCTTTCCGCGGGTGGACAAAAGACTCATTGAAAGGCGATAACTTTACACCTTTGAATCATACCCGACGCTCCTTTTTCAGCCATTTGAACAAGCATCACGAGCCACTTGACATTCTCCCAAAGCCAGTGTTAAAGTCTAATATCTCAATGCGTGATGTTCTAAGGCGTAAGCAAGTTCCAAAGGAAGATGTTGTTGCTCCCCAAAGACAGAGCCTTGAAGGGCAAATACCGAGGCGAAATGTGAATTTGAGCCAAATGTTGAAGGAACTAAAGCAGGATGTGAAGTTTTCGGCACGGGGAGAGAAAGAGGAGGTACATGAAGAGAAACAGTACGTCACGCAGAGGAGAAAGTTTGGGTTCATACACATTACACTTCCACTGCAGAAGCAGAGCAAGAAAAAATCCAGCCTTTAA

Protein Analysis

518

Amino Acids

58.48

Weight (kDa)

6.71

Isoelectric Point (pI)

57.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 57 - 140 1.2e-10 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 736, 795
AccII CGCG 1 cut(s) 1152
AciI CCGC 6 cut(s) 212, 311, 406, 866, 1150, 1152
AclWI GGATC 8 cut(s) 61, 74, 113, 205, 333, 382, 395, 571
AcsI RAATTY 4 cut(s) 100, 278, 935, 1387
AcuI CTGAAG 3 cut(s) 398, 783, 1077
AfaI GTAC 4 cut(s) 58, 141, 1458, 1475
AfiI CCNNNNNNNGG 1 cut(s) 194
AgeI ACCGGT 1 cut(s) 673
AjnI CCWGG 3 cut(s) 61, 142, 918
AlwI GGATC 8 cut(s) 61, 74, 113, 205, 333, 382, 395, 571
ApeKI GCWGC 8 cut(s) 19, 308, 409, 833, 854, 863, 1091, 1133
ApoI RAATTY 4 cut(s) 100, 278, 935, 1387
AsiGI ACCGGT 1 cut(s) 673
Asp700I GAANNNNTTC 1 cut(s) 626
AspS9I GGNCC 1 cut(s) 671
AsuC2I CCSGG 3 cut(s) 54, 90, 106
AsuHPI GGTGA 3 cut(s) 226, 287, 923
AvaII GGWCC 1 cut(s) 671
BamHI GGATCC 1 cut(s) 66
BarI GAAGNNNNNNTAC 2 cut(s) 1353, 1385
BauI CACGAG 1 cut(s) 1241
BbsI GAAGAC 1 cut(s) 644
BbvCI CCTCAGC 2 cut(s) 42, 687
BbvI GCAGC 8 cut(s) 31, 320, 421, 820, 841, 875, 1078, 1145
BccI CCATC 3 cut(s) 492, 514, 748
BciT130I CCWGG 3 cut(s) 63, 144, 920
BcnI CCSGG 3 cut(s) 54, 90, 106
BfaI CTAG 2 cut(s) 683, 837
BfmI CTRYAG 3 cut(s) 788, 813, 1523
BfuAI ACCTGC 2 cut(s) 736, 795
BglII AGATCT 1 cut(s) 959
BisI GCNGC 9 cut(s) 20, 309, 407, 410, 834, 855, 864, 1092, 1134
BlpI GCTNAGC 1 cut(s) 648
BlsI GCNGC 9 cut(s) 21, 310, 408, 411, 835, 856, 865, 1093, 1135
Bme1390I CCNGG 6 cut(s) 54, 63, 90, 106, 144, 920
Bme18I GGWCC 1 cut(s) 671
BmgT120I GGNCC 1 cut(s) 671
BmiI GGNNCC 2 cut(s) 68, 246
BmrFI CCNGG 6 cut(s) 54, 63, 90, 106, 144, 920
BmsI GCATC 3 cut(s) 31, 422, 1246
BpiI GAAGAC 1 cut(s) 644
Bpu10I CCTNAGC 2 cut(s) 42, 687
Bpu1102I GCTNAGC 1 cut(s) 648
BpuEI CTTGAG 3 cut(s) 338, 653, 1064
BpuMI CCSGG 3 cut(s) 54, 90, 106
Bsa29I ATCGAT 1 cut(s) 177
BsaJI CCNNGG 5 cut(s) 62, 558, 1005, 1150, 1373
BsaWI WCCGGW 2 cut(s) 121, 673
BsaXI ACNNNNNCTCC 2 cut(s) 172, 202
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 1 cut(s) 673
Bse1I ACTGG 2 cut(s) 580, 1268
Bse3DI GCAATG 1 cut(s) 478
BseBI CCWGG 3 cut(s) 63, 144, 920
BseCI ATCGAT 1 cut(s) 177
BseDI CCNNGG 5 cut(s) 62, 558, 1005, 1150, 1373
BseGI GGATG 3 cut(s) 361, 902, 1426
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 1 cut(s) 478
BseMII CTCAG 7 cut(s) 33, 87, 419, 460, 678, 797, 997
BseNI ACTGG 2 cut(s) 580, 1268
BseRI GAGGAG 6 cut(s) 29, 177, 314, 704, 1466, 1501
BseXI GCAGC 8 cut(s) 31, 320, 421, 820, 841, 875, 1078, 1145
Bsh1236I CGCG 1 cut(s) 1152
BshTI ACCGGT 1 cut(s) 673
BshVI ATCGAT 1 cut(s) 177
BsiSI CCGG 5 cut(s) 53, 90, 105, 122, 674
BslI CCNNNNNNNGG 1 cut(s) 194
Bsp1720I GCTNAGC 1 cut(s) 648
Bsp19I CCATGG 1 cut(s) 558
BspACI CCGC 6 cut(s) 212, 311, 406, 866, 1150, 1152
BspCNI CTCAG 7 cut(s) 34, 88, 420, 459, 679, 798, 996
BspDI ATCGAT 1 cut(s) 177
BspFNI CGCG 1 cut(s) 1152
BspLI GGNNCC 2 cut(s) 68, 246
BspMAI CTGCAG 3 cut(s) 792, 817, 1527
BspMI ACCTGC 2 cut(s) 736, 795
BspPI GGATC 8 cut(s) 61, 74, 113, 205, 333, 382, 395, 571
BsrDI GCAATG 1 cut(s) 478
BsrFI RCCGGY 1 cut(s) 673
BsrI ACTGG 2 cut(s) 580, 1268
BssAI RCCGGY 1 cut(s) 673
BssECI CCNNGG 5 cut(s) 62, 558, 1005, 1150, 1373
BssSI CACGAG 1 cut(s) 1241
BssT1I CCWWGG 1 cut(s) 558
Bst2BI CACGAG 1 cut(s) 1241
Bst2UI CCWGG 3 cut(s) 63, 144, 920
Bst4CI ACNGT 3 cut(s) 735, 803, 1473
Bst6I CTCTTC 2 cut(s) 612, 1458
BstC8I GCNNGC 3 cut(s) 306, 534, 1080
BstDSI CCRYGG 3 cut(s) 558, 1005, 1150
BstF5I GGATG 3 cut(s) 361, 902, 1426
BstFNI CGCG 1 cut(s) 1152
BstMWI GCNNNNNNNGC 7 cut(s) 415, 860, 863, 869, 1088, 1142, 1243
BstNI CCWGG 3 cut(s) 63, 144, 920
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 6 cut(s) 52, 61, 88, 104, 142, 918
BstSFI CTRYAG 3 cut(s) 788, 813, 1523
BstUI CGCG 1 cut(s) 1152
BstV1I GCAGC 8 cut(s) 31, 320, 421, 820, 841, 875, 1078, 1145
BstV2I GAAGAC 1 cut(s) 644
BstX2I RGATCY 3 cut(s) 66, 325, 959
BstYI RGATCY 3 cut(s) 66, 325, 959
Bsu15I ATCGAT 1 cut(s) 177
BsuTUI ATCGAT 1 cut(s) 177
BtgI CCRYGG 3 cut(s) 558, 1005, 1150
BtsCI GGATG 3 cut(s) 361, 902, 1426
BtsI GCAGTG 1 cut(s) 1520
BtsIMutI CAGTG 3 cut(s) 425, 1275, 1520
BveI ACCTGC 2 cut(s) 736, 795
Cac8I GCNNGC 3 cut(s) 306, 534, 1080
Cfr10I RCCGGY 1 cut(s) 673
Cfr13I GGNCC 1 cut(s) 671
Cfr42I CCGCGG 1 cut(s) 1153
ClaI ATCGAT 1 cut(s) 177
CseI GACGC 1 cut(s) 1218
Csp6I GTAC 4 cut(s) 57, 140, 1457, 1474
CspAI ACCGGT 1 cut(s) 673
CviAII CATG 6 cut(s) 35, 71, 305, 496, 559, 1460
CviQI GTAC 4 cut(s) 57, 140, 1457, 1474
DraI TTTAAA 1 cut(s) 783
Eam1104I CTCTTC 2 cut(s) 612, 1458
EarI CTCTTC 2 cut(s) 612, 1458
Eco130I CCWWGG 1 cut(s) 558
Eco32I GATATC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 671
Eco57I CTGAAG 3 cut(s) 398, 783, 1077
EcoRI GAATTC 1 cut(s) 100
EcoRII CCWGG 3 cut(s) 61, 142, 918
EcoRV GATATC 1 cut(s) 457
EcoT14I CCWWGG 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 558
FaeI CATG 6 cut(s) 38, 74, 308, 499, 562, 1463
FaiI YATR 9 cut(s) 36, 72, 306, 497, 560, 660, 1203, 1461, 1505
FalI AAGNNNNNCTT 2 cut(s) 615, 647
FatI CATG 6 cut(s) 34, 70, 304, 495, 558, 1459
FauI CCCGC 1 cut(s) 1145
Fnu4HI GCNGC 9 cut(s) 20, 309, 407, 410, 834, 855, 864, 1092, 1134
FokI GGATG 3 cut(s) 368, 889, 1433
Fsp4HI GCNGC 9 cut(s) 20, 309, 407, 410, 834, 855, 864, 1092, 1134
FspBI CTAG 2 cut(s) 683, 837
GluI GCNGC 9 cut(s) 20, 309, 407, 410, 834, 855, 864, 1092, 1134
HapII CCGG 5 cut(s) 53, 90, 105, 122, 674
HgaI GACGC 1 cut(s) 1218
Hin1II CATG 6 cut(s) 38, 74, 308, 499, 562, 1463
HindIII AAGCTT 2 cut(s) 254, 1040
HpaII CCGG 5 cut(s) 53, 90, 105, 122, 674
HphI GGTGA 3 cut(s) 226, 287, 923
Hpy166II GTNNAC 2 cut(s) 671, 1158
Hpy188III TCNNGA 9 cut(s) 79, 206, 506, 517, 725, 947, 985, 1020, 1241
Hpy8I GTNNAC 2 cut(s) 671, 1158
Hpy99I CGWCG 1 cut(s) 1212
HpyAV CCTTC 4 cut(s) 316, 1052, 1355, 1399
HpyCH4III ACNGT 3 cut(s) 735, 803, 1473
HpyCH4IV ACGT 2 cut(s) 656, 1476
HpyCH4V TGCA 5 cut(s) 308, 790, 815, 1133, 1525
HpyF10VI GCNNNNNNNGC 7 cut(s) 415, 860, 863, 869, 1088, 1142, 1243
HpySE526I ACGT 2 cut(s) 656, 1476
Hsp92II CATG 6 cut(s) 38, 74, 308, 499, 562, 1463
KspI CCGCGG 1 cut(s) 1153
LmnI GCTCC 6 cut(s) 16, 301, 313, 742, 1217, 1345
Lsp1109I GCAGC 8 cut(s) 31, 320, 421, 820, 841, 875, 1078, 1145
LweI GCATC 3 cut(s) 31, 422, 1246
MaeI CTAG 2 cut(s) 683, 837
MaeII ACGT 2 cut(s) 656, 1476
MaeIII GTNAC 3 cut(s) 214, 967, 1477
MboII GAAGA 7 cut(s) 407, 629, 649, 860, 1048, 1340, 1475
MflI RGATCY 3 cut(s) 66, 325, 959
MluCI AATT 8 cut(s) 28, 100, 147, 278, 524, 888, 935, 1387
MlyI GAGTC 4 cut(s) 211, 706, 975, 1160
MmeI TCCRAC 1 cut(s) 680
MroXI GAANNNNTTC 1 cut(s) 626
MseI TTAA 9 cut(s) 288, 705, 737, 750, 782, 1065, 1097, 1274, 1555
MslI CAYNNNNRTG 1 cut(s) 430
MspA1I CMGCKG 4 cut(s) 311, 866, 1136, 1152
MspI CCGG 5 cut(s) 53, 90, 105, 122, 674
MspR9I CCNGG 6 cut(s) 54, 63, 90, 106, 144, 920
MssI GTTTAAAC 1 cut(s) 783
MvaI CCWGG 3 cut(s) 63, 144, 920
MvnI CGCG 1 cut(s) 1152
MwoI GCNNNNNNNGC 7 cut(s) 415, 860, 863, 869, 1088, 1142, 1243
NciI CCSGG 3 cut(s) 54, 90, 106
NcoI CCATGG 1 cut(s) 558
NlaIII CATG 6 cut(s) 38, 74, 308, 499, 562, 1463
NlaIV GGNNCC 2 cut(s) 68, 246
NmuCI GTSAC 3 cut(s) 214, 967, 1477
NspI RCATGY 1 cut(s) 308
PaeI GCATGC 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 626
PfeI GAWTC 7 cut(s) 49, 82, 222, 544, 593, 929, 1198
PinAI ACCGGT 1 cut(s) 673
PkrI GCNGC 9 cut(s) 21, 310, 408, 411, 835, 856, 865, 1093, 1135
PleI GAGTC 4 cut(s) 211, 705, 975, 1160
PmeI GTTTAAAC 1 cut(s) 783
PpsI GAGTC 4 cut(s) 211, 705, 975, 1160
Psp6I CCWGG 3 cut(s) 61, 142, 918
PspGI CCWGG 3 cut(s) 61, 142, 918
PspN4I GGNNCC 2 cut(s) 68, 246
PspPI GGNCC 1 cut(s) 671
PstI CTGCAG 3 cut(s) 792, 817, 1527
PsuI RGATCY 3 cut(s) 66, 325, 959
PvuII CAGCTG 1 cut(s) 1136
RsaI GTAC 4 cut(s) 58, 141, 1458, 1475
RsaNI GTAC 4 cut(s) 57, 140, 1457, 1474
RseI CAYNNNNRTG 1 cut(s) 430
SacII CCGCGG 1 cut(s) 1153
SaqAI TTAA 9 cut(s) 288, 705, 737, 750, 782, 1065, 1097, 1274, 1555
SatI GCNGC 9 cut(s) 20, 309, 407, 410, 834, 855, 864, 1092, 1134
Sau96I GGNCC 1 cut(s) 671
SchI GAGTC 4 cut(s) 211, 706, 975, 1160
ScrFI CCNGG 6 cut(s) 54, 63, 90, 106, 144, 920
SfaNI GCATC 3 cut(s) 31, 422, 1246
SfcI CTRYAG 3 cut(s) 788, 813, 1523
Sfr303I CCGCGG 1 cut(s) 1153
SgrBI CCGCGG 1 cut(s) 1153
SinI GGWCC 1 cut(s) 671
SmiMI CAYNNNNRTG 1 cut(s) 430
SmlI CTYRAG 3 cut(s) 317, 632, 1043
SmoI CTYRAG 3 cut(s) 317, 632, 1043
SphI GCATGC 1 cut(s) 308
Sse9I AATT 8 cut(s) 28, 100, 147, 278, 524, 888, 935, 1387
SsiI CCGC 6 cut(s) 212, 311, 406, 866, 1150, 1152
SspMI CTAG 2 cut(s) 683, 837
StyD4I CCNGG 6 cut(s) 52, 61, 88, 104, 142, 918
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 3 cut(s) 735, 803, 1473
TaiI ACGT 2 cut(s) 659, 1479
TaqI TCGA 2 cut(s) 177, 220
TasI AATT 8 cut(s) 28, 100, 147, 278, 524, 888, 935, 1387
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 7 cut(s) 49, 82, 222, 544, 593, 929, 1198
Tru1I TTAA 9 cut(s) 288, 705, 737, 750, 782, 1065, 1097, 1274, 1555
Tru9I TTAA 9 cut(s) 288, 705, 737, 750, 782, 1065, 1097, 1274, 1555
TscAI CASTG 3 cut(s) 432, 1275, 1527
TseFI GTSAC 3 cut(s) 214, 967, 1477
TseI GCWGC 8 cut(s) 19, 308, 409, 833, 854, 863, 1091, 1133
Tsp45I GTSAC 3 cut(s) 214, 967, 1477
TspDTI ATGAA 7 cut(s) 482, 557, 626, 744, 786, 1476, 1492
TspGWI ACGGA 1 cut(s) 1022
TspRI CASTG 3 cut(s) 432, 1275, 1527
VpaK11BI GGWCC 1 cut(s) 671
XapI RAATTY 4 cut(s) 100, 278, 935, 1387
XceI RCATGY 1 cut(s) 308
XmnI GAANNNNTTC 1 cut(s) 626
XspI CTAG 2 cut(s) 683, 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.