AT2G43710

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0014984
Physical Location & Seq
Forward (+)
18119873 .. 18122889
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G43710.2

Sequence Viewer

Length: 1206 bp
ATGGCTCTAAAGTTTAACCCTTTGGTGGCATCTCAGCCTTACAAATTCCCTTCCTCGACTCGTCCGCCAACTCCTTCTTTCAGATCTCCCAAGTTCCTCTGCCTCGCTTCTTCTTCTCCGGCTCTCAGCTCCGGCCCCAAGGAGGTTGAGAGTTTGAAGAAACCATTTACGCCACCCAGGGAAGTGCATGTTCAAGTCTTGCACTCCATGCCACCTCAAAAGATCGAGATCTTCAAATCTATGGAAAACTGGGCCGAGGAGAACCTTCTGATTCACCTCAAGGATGTGGAGAAGTCTTGGCAACCCCAGGATTTCTTGCCTGACCCTGCATCAGATGGGTTTGAAGATCAGGTAAGAGAGTTAAGAGAGAGGGCTAGAGAGCTCCCTGATGATTACTTTGTTGTTTTGGTGGGGGACATGATCACAGAAGAAGCACTTCCGACCTATCAAACTATGTTGAACACTTTGGATGGAGTTAGGGATGAAACAGGTGCTAGTCCTACTTCATGGGCTATTTGGACCAGAGCTTGGACTGCAGAAGAAAACCGACATGGCGATCTTCTGAATAAATACCTTTACTTGTCTGGTCGTGTTGACATGAGGCAGATCGAAAAGACCATTCAGTACTTGATTGGATCTGGAATGGATCCGCGGACAGAGAATAACCCCTACCTTGGCTTCATCTATACGTCATTCCAAGAAAGAGCGACATTCATCTCTCACGGAAACACAGCCCGCCAAGCCAAAGAGCACGGGGACATCAAACTAGCCCAAATATGTGGCACAATAGCTGCAGACGAGAAGCGTCATGAAACAGCATACACCAAGATAGTTGAAAAGCTCTTTGAGATTGATCCTGATGGTACTGTCATGGCTTTTGCAGACATGATGAGAAAGAAAATCTCAATGCCTGCTCACTTGATGTATGATGGGCGCAACGACAACCTCTTTGACAACTTCTCTTCCGTGGCTCAGAGGCTCGGTGTTTACACCGCCAAAGACTATGCAGACATTCTTGAGTTTCTGGTTGGTAGGTGGAAAATCCAGGACTTAACCGGGCTTTCAGGTGAAGGAAACAAAGCACAAGACTATTTATGCGGGTTGGCTCCAAGGATCAAGAGATTGGATGAGAGAGCTCAAGCAAGAGCCAAGAAAGGACCCAAGATTCCTTTCAGTTGGATACACGACAGAGAAGTGCAGCTCTAA

Protein Analysis

401

Amino Acids

45.69

Weight (kDa)

6.05

Isoelectric Point (pI)

36.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 74 - 395 2.9e-158 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43710 AT2G43710
fragaria_vesca FvH4_4g10110 FvH4_4g10110
malus_domestica MD04G1048200.v1.1
prunus_persica Prupe.1G203700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0405901
rosa_laevigata RLG00000008819
rosa_multiflora Rmu_sc0001200.1_g000011
rosa_roxburghii Rroxscaffold_5G00350440
rosa_rugosa Rorug04G0062600
rosa_samantha Rh4AG134100 Rh4BG129200 Rh4CG142000 Rh4DG128500
rosa_wichuraiana Rw4G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 528
AccII CGCG 1 cut(s) 652
AciI CCGC 6 cut(s) 65, 650, 652, 736, 993, 1098
AclWI GGATC 5 cut(s) 641, 643, 654, 848, 1121
AcsI RAATTY 1 cut(s) 44
AfaI GTAC 2 cut(s) 626, 865
AfiI CCNNNNNNNGG 4 cut(s) 25, 142, 528, 674
AgsI TTSAA 6 cut(s) 157, 194, 235, 344, 460, 836
AjnI CCWGG 3 cut(s) 176, 306, 1044
AjuI GAANNNNNNNTTGG 2 cut(s) 1154, 1186
AluBI AGCT 7 cut(s) 129, 382, 527, 791, 841, 1136, 1201
AluI AGCT 7 cut(s) 129, 382, 527, 791, 841, 1136, 1201
Alw21I GWGCWC 3 cut(s) 384, 753, 1138
AlwI GGATC 5 cut(s) 641, 643, 654, 848, 1121
AoxI GGCC 2 cut(s) 133, 252
ApeKI GCWGC 2 cut(s) 791, 1198
ApoI RAATTY 1 cut(s) 44
ArsI GACNNNNNNTTYG 2 cut(s) 932, 964
Asp700I GAANNNNTTC 1 cut(s) 435
AspLEI GCGC 1 cut(s) 936
AspS9I GGNCC 4 cut(s) 134, 252, 519, 1157
AsuC2I CCSGG 1 cut(s) 1057
AsuHPI GGTGA 2 cut(s) 266, 1079
AvaII GGWCC 2 cut(s) 519, 1157
BamHI GGATCC 1 cut(s) 646
BanII GRGCYC 2 cut(s) 384, 1138
Bbv12I GWGCWC 3 cut(s) 384, 753, 1138
BbvI GCAGC 1 cut(s) 778
BccI CCATC 4 cut(s) 329, 464, 854, 923
BciT130I CCWGG 3 cut(s) 178, 308, 1046
BciVI GTATCC 1 cut(s) 1173
BclI TGATCA 1 cut(s) 420
BcnI CCSGG 1 cut(s) 1057
BfaI CTAG 3 cut(s) 375, 495, 767
BfmI CTRYAG 2 cut(s) 534, 792
BfuI GTATCC 1 cut(s) 1173
BglII AGATCT 2 cut(s) 83, 228
BisI GCNGC 2 cut(s) 792, 1199
BlsI GCNGC 2 cut(s) 793, 1200
BmcAI AGTACT 1 cut(s) 626
Bme1390I CCNGG 4 cut(s) 178, 308, 1046, 1057
Bme18I GGWCC 2 cut(s) 519, 1157
BmgT120I GGNCC 4 cut(s) 134, 252, 519, 1157
BmiI GGNNCC 4 cut(s) 136, 648, 1107, 1159
BmrFI CCNGG 4 cut(s) 178, 308, 1046, 1057
BmrI ACTGGG 1 cut(s) 259
BmsI GCATC 2 cut(s) 38, 338
BmuI ACTGGG 1 cut(s) 259
BpuEI CTTGAG 3 cut(s) 263, 1037, 1122
BpuMI CCSGG 1 cut(s) 1057
BsaBI GATNNNNATC 1 cut(s) 227
BsaJI CCNNGG 9 cut(s) 138, 176, 177, 255, 306, 650, 673, 966, 1109
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 142, 528, 674
Bse1I ACTGG 1 cut(s) 254
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 3 cut(s) 178, 308, 1046
BseDI CCNNGG 9 cut(s) 138, 176, 177, 255, 306, 650, 673, 966, 1109
BseGI GGATG 4 cut(s) 289, 475, 487, 1132
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 4 cut(s) 25, 142, 528, 674
BseMII CTCAG 3 cut(s) 47, 139, 986
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 1 cut(s) 272
BseXI GCAGC 1 cut(s) 778
Bsh1236I CGCG 1 cut(s) 652
BshFI GGCC 2 cut(s) 135, 254
BsiHKAI GWGCWC 3 cut(s) 384, 753, 1138
BsiSI CCGG 3 cut(s) 119, 132, 1056
BslFI GGGAC 2 cut(s) 428, 770
BslI CCNNNNNNNGG 4 cut(s) 25, 142, 528, 674
BsmFI GGGAC 2 cut(s) 428, 770
BsnI GGCC 2 cut(s) 135, 254
Bsp1286I GDGCHC 3 cut(s) 384, 753, 1138
BspACI CCGC 6 cut(s) 65, 650, 652, 736, 993, 1098
BspANI GGCC 2 cut(s) 135, 254
BspCNI CTCAG 3 cut(s) 46, 138, 985
BspFNI CGCG 1 cut(s) 652
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 4 cut(s) 136, 648, 1107, 1159
BspMAI CTGCAG 2 cut(s) 538, 796
BspPI GGATC 5 cut(s) 641, 643, 654, 848, 1121
BsrI ACTGG 1 cut(s) 254
BssECI CCNNGG 9 cut(s) 138, 176, 177, 255, 306, 650, 673, 966, 1109
BssT1I CCWWGG 3 cut(s) 138, 673, 1109
Bst2UI CCWGG 3 cut(s) 178, 308, 1046
Bst4CI ACNGT 1 cut(s) 868
Bst6I CTCTTC 1 cut(s) 967
BstAPI GCANNNNNTGC 1 cut(s) 208
BstC8I GCNNGC 2 cut(s) 736, 912
BstDEI CTNAG 3 cut(s) 33, 125, 972
BstDSI CCRYGG 2 cut(s) 650, 966
BstF5I GGATG 4 cut(s) 289, 475, 487, 1132
BstFNI CGCG 1 cut(s) 652
BstHHI GCGC 1 cut(s) 936
BstMWI GCNNNNNNNGC 3 cut(s) 208, 533, 740
BstNI CCWGG 3 cut(s) 178, 308, 1046
BstNSI RCATGY 1 cut(s) 191
BstSCI CCNGG 4 cut(s) 176, 306, 1044, 1055
BstSFI CTRYAG 2 cut(s) 534, 792
BstUI CGCG 1 cut(s) 652
BstV1I GCAGC 1 cut(s) 778
BstX2I RGATCY 4 cut(s) 83, 228, 635, 646
BstXI CCANNNNNNTGG 1 cut(s) 779
BstYI RGATCY 4 cut(s) 83, 228, 635, 646
BsuI GTATCC 1 cut(s) 1173
BsuRI GGCC 2 cut(s) 135, 254
BtgI CCRYGG 2 cut(s) 650, 966
BtsCI GGATG 4 cut(s) 289, 475, 487, 1132
Cac8I GCNNGC 2 cut(s) 736, 912
CciI TCATGA 1 cut(s) 808
CfoI GCGC 1 cut(s) 936
Cfr13I GGNCC 4 cut(s) 134, 252, 519, 1157
Cfr42I CCGCGG 1 cut(s) 653
CseI GACGC 1 cut(s) 794
Csp6I GTAC 2 cut(s) 625, 864
CviAII CATG 9 cut(s) 188, 208, 418, 507, 551, 598, 809, 871, 886
CviQI GTAC 2 cut(s) 625, 864
DdeI CTNAG 3 cut(s) 33, 125, 972
Eam1104I CTCTTC 1 cut(s) 967
EarI CTCTTC 1 cut(s) 967
EciI GGCGGA 1 cut(s) 54
Ecl136II GAGCTC 2 cut(s) 382, 1136
Eco130I CCWWGG 3 cut(s) 138, 673, 1109
Eco24I GRGCYC 2 cut(s) 384, 1138
Eco47I GGWCC 2 cut(s) 519, 1157
Eco53kI GAGCTC 2 cut(s) 382, 1136
EcoICRI GAGCTC 2 cut(s) 382, 1136
EcoO109I RGGNCCY 1 cut(s) 1157
EcoRII CCWGG 3 cut(s) 176, 306, 1044
EcoT14I CCWWGG 3 cut(s) 138, 673, 1109
EcoT38I GRGCYC 2 cut(s) 384, 1138
ErhI CCWWGG 3 cut(s) 138, 673, 1109
FaeI CATG 9 cut(s) 191, 211, 421, 510, 554, 601, 812, 874, 889
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 2 cut(s) 428, 770
FatI CATG 9 cut(s) 187, 207, 417, 506, 550, 597, 808, 870, 885
FauI CCCGC 2 cut(s) 743, 1091
FbaI TGATCA 1 cut(s) 420
Fnu4HI GCNGC 2 cut(s) 792, 1199
FokI GGATG 4 cut(s) 296, 482, 494, 1139
FriOI GRGCYC 2 cut(s) 384, 1138
Fsp4HI GCNGC 2 cut(s) 792, 1199
FspBI CTAG 3 cut(s) 375, 495, 767
GlaI GCGC 1 cut(s) 935
GluI GCNGC 2 cut(s) 792, 1199
HaeIII GGCC 2 cut(s) 135, 254
HapII CCGG 3 cut(s) 119, 132, 1056
HgaI GACGC 1 cut(s) 794
HhaI GCGC 1 cut(s) 936
Hin1II CATG 9 cut(s) 191, 211, 421, 510, 554, 601, 812, 874, 889
Hin6I GCGC 1 cut(s) 934
HinP1I GCGC 1 cut(s) 934
HincII GTYRAC 1 cut(s) 595
HindII GTYRAC 1 cut(s) 595
HinfI GANTC 3 cut(s) 58, 271, 1165
HpaII CCGG 3 cut(s) 119, 132, 1056
HphI GGTGA 2 cut(s) 266, 1079
Hpy166II GTNNAC 2 cut(s) 595, 988
Hpy188I TCNGA 6 cut(s) 83, 270, 334, 441, 564, 975
Hpy188III TCNNGA 6 cut(s) 226, 639, 809, 857, 1016, 1117
Hpy8I GTNNAC 2 cut(s) 595, 988
HpyAV CCTTC 4 cut(s) 60, 84, 275, 1064
HpyCH4III ACNGT 1 cut(s) 868
HpyCH4IV ACGT 1 cut(s) 689
HpyCH4V TGCA 8 cut(s) 187, 202, 329, 536, 794, 881, 1007, 1198
HpyF10VI GCNNNNNNNGC 3 cut(s) 208, 533, 740
HpyF3I CTNAG 3 cut(s) 33, 125, 972
HpySE526I ACGT 1 cut(s) 689
Hsp92II CATG 9 cut(s) 191, 211, 421, 510, 554, 601, 812, 874, 889
HspAI GCGC 1 cut(s) 934
Ksp22I TGATCA 1 cut(s) 420
KspI CCGCGG 1 cut(s) 653
LmnI GCTCC 3 cut(s) 134, 387, 1111
Lsp1109I GCAGC 1 cut(s) 778
LweI GCATC 2 cut(s) 38, 338
MaeI CTAG 3 cut(s) 375, 495, 767
MaeII ACGT 1 cut(s) 689
MboII GAAGA 9 cut(s) 102, 105, 169, 223, 356, 440, 551, 551, 954
MflI RGATCY 4 cut(s) 83, 228, 635, 646
MhlI GDGCHC 3 cut(s) 384, 753, 1138
MluCI AATT 1 cut(s) 44
MlyI GAGTC 1 cut(s) 52
MmeI TCCRAC 2 cut(s) 464, 1157
MroXI GAANNNNTTC 1 cut(s) 435
MseI TTAA 3 cut(s) 15, 362, 1052
MspA1I CMGCKG 1 cut(s) 652
MspI CCGG 3 cut(s) 119, 132, 1056
MspR9I CCNGG 4 cut(s) 178, 308, 1046, 1057
MvaI CCWGG 3 cut(s) 178, 308, 1046
MvnI CGCG 1 cut(s) 652
MwoI GCNNNNNNNGC 3 cut(s) 208, 533, 740
NciI CCSGG 1 cut(s) 1057
NlaIII CATG 9 cut(s) 191, 211, 421, 510, 554, 601, 812, 874, 889
NlaIV GGNNCC 4 cut(s) 136, 648, 1107, 1159
NmeAIII GCCGAG 1 cut(s) 280
NspI RCATGY 1 cut(s) 191
PagI TCATGA 1 cut(s) 808
PasI CCCWGGG 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 435
PfeI GAWTC 2 cut(s) 271, 1165
PflMI CCANNNNNTGG 1 cut(s) 528
PfoI TCCNGGA 1 cut(s) 1044
PkrI GCNGC 2 cut(s) 793, 1200
PleI GAGTC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 52
PpuMI RGGWCCY 1 cut(s) 1157
Psp124BI GAGCTC 2 cut(s) 384, 1138
Psp5II RGGWCCY 1 cut(s) 1157
Psp6I CCWGG 3 cut(s) 176, 306, 1044
PspGI CCWGG 3 cut(s) 176, 306, 1044
PspN4I GGNNCC 4 cut(s) 136, 648, 1107, 1159
PspPI GGNCC 4 cut(s) 134, 252, 519, 1157
PspPPI RGGWCCY 1 cut(s) 1157
PstI CTGCAG 2 cut(s) 538, 796
PsuI RGATCY 4 cut(s) 83, 228, 635, 646
RsaI GTAC 2 cut(s) 626, 865
RsaNI GTAC 2 cut(s) 625, 864
SacI GAGCTC 2 cut(s) 384, 1138
SacII CCGCGG 1 cut(s) 653
SaqAI TTAA 3 cut(s) 15, 362, 1052
SatI GCNGC 2 cut(s) 792, 1199
Sau96I GGNCC 4 cut(s) 134, 252, 519, 1157
ScaI AGTACT 1 cut(s) 626
SchI GAGTC 1 cut(s) 52
ScrFI CCNGG 4 cut(s) 178, 308, 1046, 1057
SduI GDGCHC 3 cut(s) 384, 753, 1138
SfaNI GCATC 2 cut(s) 38, 338
SfcI CTRYAG 2 cut(s) 534, 792
Sfr303I CCGCGG 1 cut(s) 653
SgrBI CCGCGG 1 cut(s) 653
SinI GGWCC 2 cut(s) 519, 1157
SmlI CTYRAG 3 cut(s) 278, 1016, 1137
SmoI CTYRAG 3 cut(s) 278, 1016, 1137
Sse9I AATT 1 cut(s) 44
SsiI CCGC 6 cut(s) 65, 650, 652, 736, 993, 1098
SspMI CTAG 3 cut(s) 375, 495, 767
SstI GAGCTC 2 cut(s) 384, 1138
StyD4I CCNGG 4 cut(s) 176, 306, 1044, 1055
StyI CCWWGG 3 cut(s) 138, 673, 1109
TaaI ACNGT 1 cut(s) 868
TaiI ACGT 1 cut(s) 692
TaqI TCGA 3 cut(s) 56, 225, 609
TasI AATT 1 cut(s) 44
TatI WGTACW 1 cut(s) 624
TfiI GAWTC 2 cut(s) 271, 1165
Tru1I TTAA 3 cut(s) 15, 362, 1052
Tru9I TTAA 3 cut(s) 15, 362, 1052
TseI GCWGC 2 cut(s) 791, 1198
TspDTI ATGAA 5 cut(s) 495, 498, 670, 703, 825
TspGWI ACGGA 2 cut(s) 738, 955
Van91I CCANNNNNTGG 1 cut(s) 528
VpaK11BI GGWCC 2 cut(s) 519, 1157
XapI RAATTY 1 cut(s) 44
XceI RCATGY 1 cut(s) 191
XmnI GAANNNNTTC 1 cut(s) 435
XspI CTAG 3 cut(s) 375, 495, 767
ZrmI AGTACT 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.