Rh4CG142000

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0014984
Physical Location & Seq
Reverse (-)
28372832 .. 28377343
4512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG142000.1

Sequence Viewer

Length: 999 bp
ATGCCACCTCAAAAGATTGAGATCTTCAAATCCATTGAGGGTTGGGCTGAGGAGAACATCTTAGTTCACCTAAAGCCAGTTGAAAAGTGCTGGCAACCCCAGGATTTTTTGCCAGATCCTGCATCTGATGGATTTGAGGAACAAGTTAAGGAACTACGGGAGAGGGCAAAGGAGATTCCAGATGAATACTTTGTTGTTTTGGTCGGAGATATGATTACTGAAGAAGCCCTTCCAACTTATCAAACAATGCTTAATACCTTGGATGGAGTTCGGGATGAAACAGGTGCAAGCCCAACTCCTTGGGCAGTTTGGACAAGGGCATGGACAGCTGAAGAGAACAGGCACGGTGACCTTCTCAATAAGTATCTCTACCTCTCTGGCCGAGTAGACATGAGGCAAATTGAAAAGACGATTCAGTATTTGATTGGGTCTGGAATGGATCCGCGTACGGAGAACAGTCCTTACCTTGGGTTCATTTATACATCATTCCAGGAGAGGGCTACCTTCATTTCTCATGGAAATACTGCCAGGCATGCCAAGGAGTATGGGGATTTGAAGTTGGCTCAAATATGTGGCACAATAGCCTCAGATGAAAAGCGCCATGAGACTGCATATACCAAGATTGTGGAAAAGCTCTTTGAGATTGATCCCGATGGAACTGTCACATCTTTTGCTGACATGATGAAGAAGAAGATCGCTATGCCAGCTCACTTGATGTATGATGGCCGTGATGACAAACTTTTTGAGCACTTCTCAGCTGTTGCACAGCGTCTTGGTGTCTATACGGCCAAGGACTATGCAGATATATTGGAGTTCTTGGTGGGCAGGTGGAAGGTGCAGGACCTGACTGGACTTTCAGGTGAGGGTCGCAAGGCTCAAGAGTATCTGTGTGGGTTAGCCCCAAGAATTAGAAGACTAGAGGACAGAGCTCAAGCAAGGGCCAAGCAAGGACCTGCCATACCCTTCAGTTGGATCTTTGATAGAAATGTGGGACTGTGA

Protein Analysis

332

Amino Acids

37.96

Weight (kDa)

5.35

Isoelectric Point (pI)

36.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 5 - 326 1.6e-161 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43710 AT2G43710
fragaria_vesca FvH4_4g10110 FvH4_4g10110
malus_domestica MD04G1048200.v1.1
prunus_persica Prupe.1G203700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0405901
rosa_laevigata RLG00000008819
rosa_multiflora Rmu_sc0001200.1_g000011
rosa_roxburghii Rroxscaffold_5G00350440
rosa_rugosa Rorug04G0062600
rosa_samantha Rh4AG134100 Rh4BG129200 Rh4CG142000 Rh4DG128500
rosa_wichuraiana Rw4G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 816
Acc36I ACCTGC 2 cut(s) 816, 961
AccI GTMKAC 1 cut(s) 387
AccII CGCG 1 cut(s) 445
AciI CCGC 1 cut(s) 443
AclWI GGATC 5 cut(s) 110, 434, 447, 641, 980
AcoI YGGCCR 3 cut(s) 379, 724, 786
AcuI CTGAAG 3 cut(s) 240, 351, 949
AfaI GTAC 1 cut(s) 448
AfiI CCNNNNNNNGG 3 cut(s) 467, 496, 969
AgsI TTSAA 4 cut(s) 28, 83, 404, 556
AjnI CCWGG 3 cut(s) 99, 489, 527
AluBI AGCT 5 cut(s) 329, 634, 707, 758, 929
AluI AGCT 5 cut(s) 329, 634, 707, 758, 929
Alw21I GWGCWC 2 cut(s) 750, 931
Alw26I GTCTC 1 cut(s) 599
AlwI GGATC 5 cut(s) 110, 434, 447, 641, 980
AlwNI CAGNNNCTG 2 cut(s) 119, 844
AoxI GGCC 4 cut(s) 379, 724, 786, 939
ArsI GACNNNNNNTTYG 2 cut(s) 725, 757
Asp700I GAANNNNTTC 1 cut(s) 228
AspLEI GCGC 1 cut(s) 600
AspS9I GGNCC 3 cut(s) 841, 939, 950
AsuHPI GGTGA 3 cut(s) 59, 359, 872
AvaII GGWCC 2 cut(s) 841, 950
BamHI GGATCC 1 cut(s) 439
BanII GRGCYC 1 cut(s) 931
BbsI GAAGAC 1 cut(s) 919
Bbv12I GWGCWC 2 cut(s) 750, 931
BbvCI CCTCAGC 1 cut(s) 48
BccI CCATC 4 cut(s) 122, 257, 647, 716
BceAI ACGGC 2 cut(s) 711, 801
BciT130I CCWGG 3 cut(s) 101, 491, 529
BcoDI GTCTC 1 cut(s) 599
BfaI CTAG 1 cut(s) 917
BfoI RGCGCY 1 cut(s) 601
BfuAI ACCTGC 2 cut(s) 816, 961
BglII AGATCT 1 cut(s) 21
Bme1390I CCNGG 3 cut(s) 101, 491, 529
Bme18I GGWCC 2 cut(s) 841, 950
BmgT120I GGNCC 3 cut(s) 841, 939, 950
BmiI GGNNCC 1 cut(s) 441
BmrFI CCNGG 3 cut(s) 101, 491, 529
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 919
BplI GAGNNNNNCTC 2 cut(s) 737, 769
Bpu10I CCTNAGC 1 cut(s) 48
BpuEI CTTGAG 2 cut(s) 861, 915
BsaBI GATNNNNATC 1 cut(s) 20
BsaJI CCNNGG 6 cut(s) 99, 258, 299, 466, 537, 789
Bsc4I CCNNNNNNNGG 3 cut(s) 467, 496, 969
Bse1I ACTGG 2 cut(s) 77, 853
Bse8I GATNNNNATC 1 cut(s) 20
BseBI CCWGG 3 cut(s) 101, 491, 529
BseDI CCNNGG 6 cut(s) 99, 258, 299, 466, 537, 789
BseGI GGATG 2 cut(s) 268, 280
BseJI GATNNNNATC 1 cut(s) 20
BseLI CCNNNNNNNGG 3 cut(s) 467, 496, 969
BseMII CTCAG 3 cut(s) 39, 600, 768
BseNI ACTGG 2 cut(s) 77, 853
BseRI GAGGAG 1 cut(s) 65
BsgI GTGCAG 1 cut(s) 857
Bsh1236I CGCG 1 cut(s) 445
BshFI GGCC 4 cut(s) 381, 726, 788, 941
BsiHKAI GWGCWC 2 cut(s) 750, 931
BsiWI CGTACG 1 cut(s) 446
BslI CCNNNNNNNGG 3 cut(s) 467, 496, 969
BsmAI GTCTC 1 cut(s) 599
BsnI GGCC 4 cut(s) 381, 726, 788, 941
Bsp1286I GDGCHC 2 cut(s) 750, 931
Bsp143I GATC 6 cut(s) 21, 115, 439, 646, 693, 972
BspACI CCGC 1 cut(s) 443
BspANI GGCC 4 cut(s) 381, 726, 788, 941
BspCNI CTCAG 3 cut(s) 40, 599, 767
BspFNI CGCG 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 441
BspMI ACCTGC 2 cut(s) 816, 961
BspPI GGATC 5 cut(s) 110, 434, 447, 641, 980
BsrI ACTGG 2 cut(s) 77, 853
BssECI CCNNGG 6 cut(s) 99, 258, 299, 466, 537, 789
BssMI GATC 6 cut(s) 21, 115, 439, 646, 693, 972
BssT1I CCWWGG 5 cut(s) 258, 299, 466, 537, 789
Bst2UI CCWGG 3 cut(s) 101, 491, 529
Bst4CI ACNGT 4 cut(s) 347, 458, 661, 996
Bst6I CTCTTC 1 cut(s) 327
BstC8I GCNNGC 4 cut(s) 92, 289, 534, 705
BstDEI CTNAG 4 cut(s) 48, 61, 586, 754
BstEII GGTNACC 1 cut(s) 347
BstF5I GGATG 2 cut(s) 268, 280
BstFNI CGCG 1 cut(s) 445
BstH2I RGCGCY 1 cut(s) 601
BstHHI GCGC 1 cut(s) 600
BstKTI GATC 6 cut(s) 24, 118, 442, 649, 696, 975
BstMAI GTCTC 1 cut(s) 599
BstMBI GATC 6 cut(s) 21, 115, 439, 646, 693, 972
BstMWI GCNNNNNNNGC 3 cut(s) 326, 533, 704
BstNI CCWGG 3 cut(s) 101, 491, 529
BstNSI RCATGY 1 cut(s) 536
BstPI GGTNACC 1 cut(s) 347
BstSCI CCNGG 3 cut(s) 99, 489, 527
BstUI CGCG 1 cut(s) 445
BstV2I GAAGAC 1 cut(s) 919
BstX2I RGATCY 4 cut(s) 21, 115, 439, 972
BstXI CCANNNNNNTGG 2 cut(s) 300, 625
BstYI RGATCY 4 cut(s) 21, 115, 439, 972
BsuRI GGCC 4 cut(s) 381, 726, 788, 941
BtsCI GGATG 2 cut(s) 268, 280
BveI ACCTGC 2 cut(s) 816, 961
Cac8I GCNNGC 4 cut(s) 92, 289, 534, 705
CaiI CAGNNNCTG 2 cut(s) 119, 844
CfoI GCGC 1 cut(s) 600
Cfr13I GGNCC 3 cut(s) 841, 939, 950
CseI GACGC 1 cut(s) 758
Csp6I GTAC 1 cut(s) 447
CviAII CATG 6 cut(s) 321, 391, 515, 533, 602, 679
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 4 cut(s) 48, 61, 586, 754
DpnI GATC 6 cut(s) 23, 117, 441, 648, 695, 974
DpnII GATC 6 cut(s) 21, 115, 439, 646, 693, 972
EaeI YGGCCR 3 cut(s) 379, 724, 786
Eam1104I CTCTTC 1 cut(s) 327
EarI CTCTTC 1 cut(s) 327
Ecl136II GAGCTC 1 cut(s) 929
Eco130I CCWWGG 5 cut(s) 258, 299, 466, 537, 789
Eco24I GRGCYC 1 cut(s) 931
Eco47I GGWCC 2 cut(s) 841, 950
Eco53kI GAGCTC 1 cut(s) 929
Eco57I CTGAAG 3 cut(s) 240, 351, 949
Eco91I GGTNACC 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 929
EcoO109I RGGNCCY 2 cut(s) 841, 950
EcoO65I GGTNACC 1 cut(s) 347
EcoRII CCWGG 3 cut(s) 99, 489, 527
EcoT14I CCWWGG 5 cut(s) 258, 299, 466, 537, 789
EcoT38I GRGCYC 1 cut(s) 931
ErhI CCWWGG 5 cut(s) 258, 299, 466, 537, 789
FaeI CATG 6 cut(s) 324, 394, 518, 536, 605, 682
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FatI CATG 6 cut(s) 320, 390, 514, 532, 601, 678
FblI GTMKAC 1 cut(s) 387
FokI GGATG 2 cut(s) 275, 287
FriOI GRGCYC 1 cut(s) 931
FspBI CTAG 1 cut(s) 917
GlaI GCGC 1 cut(s) 599
HaeII RGCGCY 1 cut(s) 601
HaeIII GGCC 4 cut(s) 381, 726, 788, 941
HgaI GACGC 1 cut(s) 758
HhaI GCGC 1 cut(s) 600
Hin1II CATG 6 cut(s) 324, 394, 518, 536, 605, 682
Hin6I GCGC 1 cut(s) 598
HinP1I GCGC 1 cut(s) 598
HinfI GANTC 2 cut(s) 175, 412
HphI GGTGA 3 cut(s) 59, 359, 872
Hpy166II GTNNAC 2 cut(s) 67, 388
Hpy188I TCNGA 3 cut(s) 127, 206, 589
Hpy188III TCNNGA 5 cut(s) 179, 272, 432, 650, 878
Hpy8I GTNNAC 2 cut(s) 67, 388
HpyAV CCTTC 5 cut(s) 239, 362, 514, 826, 973
HpyCH4III ACNGT 4 cut(s) 347, 458, 661, 996
HpyCH4V TGCA 6 cut(s) 122, 287, 611, 764, 800, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 533, 704
HpyF3I CTNAG 4 cut(s) 48, 61, 586, 754
Hsp92II CATG 6 cut(s) 324, 394, 518, 536, 605, 682
HspAI GCGC 1 cut(s) 598
Kzo9I GATC 6 cut(s) 21, 115, 439, 646, 693, 972
LweI GCATC 1 cut(s) 131
MaeI CTAG 1 cut(s) 917
MaeIII GTNAC 2 cut(s) 347, 661
MalI GATC 6 cut(s) 23, 117, 441, 648, 695, 974
MboI GATC 6 cut(s) 21, 115, 439, 646, 693, 972
MboII GAAGA 7 cut(s) 16, 233, 344, 697, 700, 703, 924
MflI RGATCY 4 cut(s) 21, 115, 439, 972
MhlI GDGCHC 2 cut(s) 750, 931
MluCI AATT 2 cut(s) 399, 906
MmeI TCCRAC 3 cut(s) 184, 257, 950
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 2 cut(s) 147, 252
MspA1I CMGCKG 2 cut(s) 329, 758
MspR9I CCNGG 3 cut(s) 101, 491, 529
MvaI CCWGG 3 cut(s) 101, 491, 529
MvnI CGCG 1 cut(s) 445
MwoI GCNNNNNNNGC 3 cut(s) 326, 533, 704
NdeII GATC 6 cut(s) 21, 115, 439, 646, 693, 972
NlaIII CATG 6 cut(s) 324, 394, 518, 536, 605, 682
NlaIV GGNNCC 1 cut(s) 441
NmeAIII GCCGAG 1 cut(s) 407
NmuCI GTSAC 2 cut(s) 347, 661
NspI RCATGY 1 cut(s) 536
PaeI GCATGC 1 cut(s) 536
PaqCI CACCTGC 1 cut(s) 816
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 2 cut(s) 175, 412
Pfl23II CGTACG 1 cut(s) 446
PfoI TCCNGGA 1 cut(s) 489
PpuMI RGGWCCY 2 cut(s) 841, 950
Psp124BI GAGCTC 1 cut(s) 931
Psp5II RGGWCCY 2 cut(s) 841, 950
Psp6I CCWGG 3 cut(s) 99, 489, 527
PspEI GGTNACC 1 cut(s) 347
PspGI CCWGG 3 cut(s) 99, 489, 527
PspLI CGTACG 1 cut(s) 446
PspN4I GGNNCC 1 cut(s) 441
PspPI GGNCC 3 cut(s) 841, 939, 950
PspPPI RGGWCCY 2 cut(s) 841, 950
PsrI GAACNNNNNNTAC 2 cut(s) 446, 478
PstNI CAGNNNCTG 2 cut(s) 119, 844
PsuI RGATCY 4 cut(s) 21, 115, 439, 972
PvuII CAGCTG 2 cut(s) 329, 758
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
SacI GAGCTC 1 cut(s) 931
SaqAI TTAA 2 cut(s) 147, 252
Sau3AI GATC 6 cut(s) 21, 115, 439, 646, 693, 972
Sau96I GGNCC 3 cut(s) 841, 939, 950
ScrFI CCNGG 3 cut(s) 101, 491, 529
SduI GDGCHC 2 cut(s) 750, 931
SfaNI GCATC 1 cut(s) 131
SinI GGWCC 2 cut(s) 841, 950
SmlI CTYRAG 2 cut(s) 876, 930
SmoI CTYRAG 2 cut(s) 876, 930
SphI GCATGC 1 cut(s) 536
Sse9I AATT 2 cut(s) 399, 906
SsiI CCGC 1 cut(s) 443
SspMI CTAG 1 cut(s) 917
SstI GAGCTC 1 cut(s) 931
StyD4I CCNGG 3 cut(s) 99, 489, 527
StyI CCWWGG 5 cut(s) 258, 299, 466, 537, 789
TaaI ACNGT 4 cut(s) 347, 458, 661, 996
TasI AATT 2 cut(s) 399, 906
TfiI GAWTC 2 cut(s) 175, 412
Tru1I TTAA 2 cut(s) 147, 252
Tru9I TTAA 2 cut(s) 147, 252
TseFI GTSAC 2 cut(s) 347, 661
Tsp45I GTSAC 2 cut(s) 347, 661
TspDTI ATGAA 6 cut(s) 198, 291, 463, 496, 606, 698
TspGWI ACGGA 1 cut(s) 464
VpaK11BI GGWCC 2 cut(s) 841, 950
XceI RCATGY 1 cut(s) 536
XmiI GTMKAC 1 cut(s) 387
XmnI GAANNNNTTC 1 cut(s) 228
XspI CTAG 1 cut(s) 917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.