Rroxscaffold_5G00350440

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014984
Physical Location & Seq
Reverse (-)
24395234 .. 24399641
4408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00350440.1

Sequence Viewer

Length: 861 bp
ATGGCTCTCAAACTCAACGCCTTCACTACTCAATCCCCCAAGTTTGCTCTTCCCCAGATGGCCAGCCTCAGATCTCCCAAGTTCTCGATGGCCTCCACTCTCCGCTCTGGCGCCAAGGAGGTTGAGAATCTGAAGAAGCCTTTTACTCCTCCTCGGGAGGTGCATGTTCAAGTAACCCATTCCATGCCACCTCAAAAGATTGAGATCTTCAAATCCATTGAGGGTTGGGCTGAGGAGAACATCTTAATTCACCTAAAGCCAGTTGAAAAGTGCTGGCAACCCCAGGATTTTTTGCCAGATCCTGCATCTGATGGATTTGAGGAACAAGTTAAGGAACTACGGGAGAGGGCAAAGGAGATTCCAGATGAATACTTTGTTGTTTTGGTCGGAGATATGATTACTGAAGAAGCCCTTCCAACTTATCAAACAATGCTTAATACCTTGGATGGAGTTCGGGATGAAACAGGTGCAAGCCCAACTCCTTGGGCAGTTTGGACAAGGGCATGGACAGCTGAAGAGAACAGGCACGGTGACCTTCTCAATAAGTATCTCTACCTCTCTGGCCGAGTAGACATGAGGCAAATTGAAAAGACGATTCAGTATTTGATTGGGTCTGGAATGGATCCGCGTACGGAGAACAGTCCTTACCTTGGGTTCATTTATACATCATTCCAGGAGAGGGCTACCTTCATTTCTCATGGAAATACTGCCAGGCATGCCAAGGACTATGGGGATTTGAAGTTGGCTCAAATATGTGGCACAATAGCCTCAGATGAAAAGCGCCATGAGACTGCATATACTAAGATTGTGGAAAAGCTCTTTGAGATTGATCCCGATGGAACTGTCACGTCTTTTTGCTGA

Protein Analysis

286

Amino Acids

32.53

Weight (kDa)

5.49

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 66 - 285 6.7e-114 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43710 AT2G43710
fragaria_vesca FvH4_4g10110 FvH4_4g10110
malus_domestica MD04G1048200.v1.1
prunus_persica Prupe.1G203700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0405901
rosa_laevigata RLG00000008819
rosa_multiflora Rmu_sc0001200.1_g000011
rosa_roxburghii Rroxscaffold_5G00350440
rosa_rugosa Rorug04G0062600
rosa_samantha Rh4AG134100 Rh4BG129200 Rh4CG142000 Rh4DG128500
rosa_wichuraiana Rw4G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 570
AccII CGCG 1 cut(s) 628
AciI CCGC 2 cut(s) 103, 626
AclWI GGATC 4 cut(s) 293, 617, 630, 824
AcoI YGGCCR 2 cut(s) 60, 562
AcuI CTGAAG 3 cut(s) 152, 423, 534
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 1 cut(s) 631
AfiI CCNNNNNNNGG 2 cut(s) 650, 679
AgsI TTSAA 5 cut(s) 170, 211, 266, 587, 739
AjiI CACGTC 1 cut(s) 849
AjnI CCWGG 3 cut(s) 282, 672, 710
AluBI AGCT 2 cut(s) 512, 817
AluI AGCT 2 cut(s) 512, 817
Alw26I GTCTC 1 cut(s) 782
AlwI GGATC 4 cut(s) 293, 617, 630, 824
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 3 cut(s) 60, 90, 562
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 2 cut(s) 113, 783
AsuHPI GGTGA 2 cut(s) 242, 542
AvaI CYCGRG 1 cut(s) 153
BalI TGGCCA 1 cut(s) 62
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 110
BbvCI CCTCAGC 1 cut(s) 231
BccI CCATC 5 cut(s) 52, 82, 305, 440, 830
BciT130I CCWGG 3 cut(s) 284, 674, 712
BcoDI GTCTC 1 cut(s) 782
BfoI RGCGCY 2 cut(s) 114, 784
BglII AGATCT 2 cut(s) 71, 204
Bme1390I CCNGG 3 cut(s) 284, 674, 712
BmeT110I CYCGRG 1 cut(s) 153
BmgBI CACGTC 1 cut(s) 849
BmiI GGNNCC 2 cut(s) 112, 624
BmrFI CCNGG 3 cut(s) 284, 674, 712
BmsI GCATC 1 cut(s) 314
Bpu10I CCTNAGC 1 cut(s) 231
BsaBI GATNNNNATC 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 7 cut(s) 114, 152, 282, 441, 482, 649, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 650, 679
Bse1I ACTGG 1 cut(s) 260
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 3 cut(s) 284, 674, 712
BseDI CCNNGG 7 cut(s) 114, 152, 282, 441, 482, 649, 720
BseGI GGATG 2 cut(s) 451, 463
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 2 cut(s) 650, 679
BseMII CTCAG 3 cut(s) 82, 222, 783
BseNI ACTGG 1 cut(s) 260
BseRI GAGGAG 3 cut(s) 138, 141, 248
Bsh1236I CGCG 1 cut(s) 628
BshFI GGCC 3 cut(s) 62, 92, 564
BshNI GGYRCC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 153
BsiWI CGTACG 1 cut(s) 629
BslI CCNNNNNNNGG 2 cut(s) 650, 679
BsmAI GTCTC 1 cut(s) 782
BsnI GGCC 3 cut(s) 62, 92, 564
BsoBI CYCGRG 1 cut(s) 153
Bsp143I GATC 5 cut(s) 71, 204, 298, 622, 829
BspACI CCGC 2 cut(s) 103, 626
BspANI GGCC 3 cut(s) 62, 92, 564
BspCNI CTCAG 3 cut(s) 81, 223, 782
BspFNI CGCG 1 cut(s) 628
BspLI GGNNCC 2 cut(s) 112, 624
BspPI GGATC 4 cut(s) 293, 617, 630, 824
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 105
BsrI ACTGG 1 cut(s) 260
BssECI CCNNGG 7 cut(s) 114, 152, 282, 441, 482, 649, 720
BssMI GATC 5 cut(s) 71, 204, 298, 622, 829
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 5 cut(s) 114, 441, 482, 649, 720
Bst2UI CCWGG 3 cut(s) 284, 674, 712
Bst4CI ACNGT 3 cut(s) 530, 641, 844
Bst6I CTCTTC 2 cut(s) 54, 510
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 4 cut(s) 64, 275, 472, 717
BstDEI CTNAG 4 cut(s) 68, 231, 769, 801
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 2 cut(s) 451, 463
BstFNI CGCG 1 cut(s) 628
BstH2I RGCGCY 2 cut(s) 114, 784
BstHHI GCGC 2 cut(s) 113, 783
BstKTI GATC 5 cut(s) 74, 207, 301, 625, 832
BstMAI GTCTC 1 cut(s) 782
BstMBI GATC 5 cut(s) 71, 204, 298, 622, 829
BstMWI GCNNNNNNNGC 2 cut(s) 509, 716
BstNI CCWGG 3 cut(s) 284, 674, 712
BstNSI RCATGY 2 cut(s) 167, 719
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 3 cut(s) 282, 672, 710
BstUI CGCG 1 cut(s) 628
BstX2I RGATCY 4 cut(s) 71, 204, 298, 622
BstXI CCANNNNNNTGG 1 cut(s) 483
BstYI RGATCY 4 cut(s) 71, 204, 298, 622
BsuRI GGCC 3 cut(s) 62, 92, 564
BtrI CACGTC 1 cut(s) 849
BtsCI GGATG 2 cut(s) 451, 463
Cac8I GCNNGC 4 cut(s) 64, 275, 472, 717
CaiI CAGNNNCTG 1 cut(s) 302
CfoI GCGC 2 cut(s) 113, 783
Csp6I GTAC 1 cut(s) 630
CviAII CATG 7 cut(s) 164, 184, 504, 574, 698, 716, 785
CviQI GTAC 1 cut(s) 630
DdeI CTNAG 4 cut(s) 68, 231, 769, 801
DinI GGCGCC 1 cut(s) 112
DpnI GATC 5 cut(s) 73, 206, 300, 624, 831
DpnII GATC 5 cut(s) 71, 204, 298, 622, 829
EaeI YGGCCR 2 cut(s) 60, 562
Eam1104I CTCTTC 2 cut(s) 54, 510
EarI CTCTTC 2 cut(s) 54, 510
Eco130I CCWWGG 5 cut(s) 114, 441, 482, 649, 720
Eco57I CTGAAG 3 cut(s) 152, 423, 534
Eco88I CYCGRG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 530
EcoO65I GGTNACC 1 cut(s) 530
EcoRII CCWGG 3 cut(s) 282, 672, 710
EcoT14I CCWWGG 5 cut(s) 114, 441, 482, 649, 720
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 5 cut(s) 114, 441, 482, 649, 720
FaeI CATG 7 cut(s) 167, 187, 507, 577, 701, 719, 788
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FatI CATG 7 cut(s) 163, 183, 503, 573, 697, 715, 784
FblI GTMKAC 1 cut(s) 570
FokI GGATG 2 cut(s) 458, 470
GlaI GCGC 2 cut(s) 112, 782
HaeII RGCGCY 2 cut(s) 114, 784
HaeIII GGCC 3 cut(s) 62, 92, 564
HhaI GCGC 2 cut(s) 113, 783
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 7 cut(s) 167, 187, 507, 577, 701, 719, 788
Hin6I GCGC 2 cut(s) 111, 781
HinP1I GCGC 2 cut(s) 111, 781
HinfI GANTC 3 cut(s) 127, 358, 595
HphI GGTGA 2 cut(s) 242, 542
Hpy166II GTNNAC 1 cut(s) 571
Hpy188I TCNGA 5 cut(s) 71, 132, 310, 389, 772
Hpy188III TCNNGA 6 cut(s) 85, 155, 362, 455, 615, 833
Hpy8I GTNNAC 1 cut(s) 571
HpyAV CCTTC 4 cut(s) 31, 422, 545, 697
HpyCH4III ACNGT 3 cut(s) 530, 641, 844
HpyCH4IV ACGT 1 cut(s) 848
HpyCH4V TGCA 4 cut(s) 163, 305, 470, 794
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 716
HpyF3I CTNAG 4 cut(s) 68, 231, 769, 801
HpySE526I ACGT 1 cut(s) 848
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 7 cut(s) 167, 187, 507, 577, 701, 719, 788
HspAI GCGC 2 cut(s) 111, 781
KasI GGCGCC 1 cut(s) 110
Kzo9I GATC 5 cut(s) 71, 204, 298, 622, 829
LguI GCTCTTC 1 cut(s) 54
LweI GCATC 1 cut(s) 314
MaeII ACGT 1 cut(s) 848
MaeIII GTNAC 3 cut(s) 172, 530, 844
MalI GATC 5 cut(s) 73, 206, 300, 624, 831
MbiI CCGCTC 1 cut(s) 105
MboI GATC 5 cut(s) 71, 204, 298, 622, 829
MboII GAAGA 5 cut(s) 41, 145, 199, 416, 527
MflI RGATCY 4 cut(s) 71, 204, 298, 622
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 2 cut(s) 246, 582
MluNI TGGCCA 1 cut(s) 62
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 2 cut(s) 367, 440
Mox20I TGGCCA 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 411
MscI TGGCCA 1 cut(s) 62
MseI TTAA 3 cut(s) 245, 330, 435
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 512
MspR9I CCNGG 3 cut(s) 284, 674, 712
MvaI CCWGG 3 cut(s) 284, 674, 712
MvnI CGCG 1 cut(s) 628
MwoI GCNNNNNNNGC 2 cut(s) 509, 716
NarI GGCGCC 1 cut(s) 111
NdeII GATC 5 cut(s) 71, 204, 298, 622, 829
NlaIII CATG 7 cut(s) 167, 187, 507, 577, 701, 719, 788
NlaIV GGNNCC 2 cut(s) 112, 624
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 2 cut(s) 530, 844
NspI RCATGY 2 cut(s) 167, 719
PaeI GCATGC 1 cut(s) 719
PciSI GCTCTTC 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 3 cut(s) 127, 358, 595
Pfl23II CGTACG 1 cut(s) 629
PfoI TCCNGGA 1 cut(s) 672
PluTI GGCGCC 1 cut(s) 114
Psp6I CCWGG 3 cut(s) 282, 672, 710
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 3 cut(s) 282, 672, 710
PspLI CGTACG 1 cut(s) 629
PspN4I GGNNCC 2 cut(s) 112, 624
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PstNI CAGNNNCTG 1 cut(s) 302
PsuI RGATCY 4 cut(s) 71, 204, 298, 622
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 631
RsaNI GTAC 1 cut(s) 630
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 3 cut(s) 245, 330, 435
Sau3AI GATC 5 cut(s) 71, 204, 298, 622, 829
ScrFI CCNGG 3 cut(s) 284, 674, 712
SfaNI GCATC 1 cut(s) 314
SfoI GGCGCC 1 cut(s) 112
SphI GCATGC 1 cut(s) 719
Sse9I AATT 2 cut(s) 246, 582
SsiI CCGC 2 cut(s) 103, 626
SspDI GGCGCC 1 cut(s) 110
StyD4I CCNGG 3 cut(s) 282, 672, 710
StyI CCWWGG 5 cut(s) 114, 441, 482, 649, 720
TaaI ACNGT 3 cut(s) 530, 641, 844
TaiI ACGT 1 cut(s) 851
TaqI TCGA 1 cut(s) 86
TasI AATT 2 cut(s) 246, 582
TfiI GAWTC 3 cut(s) 127, 358, 595
Tru1I TTAA 3 cut(s) 245, 330, 435
Tru9I TTAA 3 cut(s) 245, 330, 435
TseFI GTSAC 2 cut(s) 530, 844
Tsp45I GTSAC 2 cut(s) 530, 844
TspDTI ATGAA 5 cut(s) 381, 474, 646, 679, 789
TspGWI ACGGA 1 cut(s) 647
XceI RCATGY 2 cut(s) 167, 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.