Rorug04G0062600

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014984
Physical Location & Seq
Forward (+)
9807109 .. 9808443
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0062600.1

Sequence Viewer

Length: 1335 bp
ATGGCTGACAAAACAACCCTCCAGTCTACCAAACTAAGAAGTAAAAAACCAAACTGGTCCGAGCTTCCAGTTGACCCTTGTGAAACAATCTTATCAAAATTGACCCCAAAATCCATACTACGATTCAAGGCAGTTTGTCCTTCATGGAACAAGGCAGCCGAATCCATCTTGCGGTCATCATCATTCACTTGCGCACAAGAACCACCATGGCTCCTCCTTCCCCAAGATGGTCCTGGTCATGATCACAGTGGGTTTCGTTTTTACAGTTTTGAAGGAAAGTCTTACAACTTGAACAAGAACATGCCTGAGGAATTGGGTCATAGTGTTTGCCTCGGATCTGATCAAAGTTGGCTGCTTCTTCTTGTCTTGCAGAACATGAATAATCTAAAGCTTCTGCTCTTTAATCCCCTTTCGGGTACACAACTTCTACTCCCACCTCTCAGTACTTTTCCAGATATACTTCGCATAGCTTATTACCATGATGATATCAACATGTATAATGATGATGACTCGTGTAAAATCAAAATCGAAGTCAAAATCAAAATCATGACCAATCCTGATCCTACTACACCACCACGATCGTTCAATTTGGTTATGAGATCAATGAAAGATTTTAAACATAAGATATTAACCAAAGTAGTTCTGTCATCACGGCCAATTCGCAACAGCAACAAAAGAAGCAGCATTGGGGTGTTTGTGATGTACAGATTCAGTATATTCTATCCGGGTTATTTTTATCTTGCATTCTGTGTGACTGCAGATGATGAGTGGAAAAAACTTGATGAACAAAGAGCGTACCAAGATATTACGAGCTATGATAATCAGCTTTATGCTTTGTGTCATGGTTACACTAGTGTTCAAGTATGGGATTTCGTGAGTTCCTTTCCTGTTAAAAAATTGGAAATCAAAGTGGATGCATCGTCCATAGTACTCCAATTAGCAGGCTTCTACAGTTGTGCTTTCATAATCAAAACAGGCGTAGATGATCTCTTCCTGGTCCTGAAGTTTTTATGTTATAGTCTCATGAAACCGGATGTATTCCATGTTTATAAACGGGATTCCAATGATAGATGGATGGCGGTTGAATCCATTGGCAATCATGCTTTGGTTTTGAGTGTGAAACAGTCTATATTAATTTCTTCAAAAGATTCTCCAGAATATGATCAGAATTCAATCTACTCTCCTTATTTACTCTCAGATAAGAGCCCTTCCTGCAGTCGTTTAGGTTTGTTTAGTTGGAGAAACAAACAAGTGAAAGAAGTCTGTGGTGGTAAACTGGCGCCTGAAACACCACAAATTTTCTTCGTTCCAAATCCCTTTTCACACATTCGATAG

Protein Analysis

444

Amino Acids

50.8

Weight (kDa)

8.84

Isoelectric Point (pI)

55.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 19 - 55 2.6e-06 F-box domain
F-box-like PF12937 19 - 55 7.3e-06 F-box-like
Beta-prop_KIB1-4 PF03478 87 - 395 5.7e-33 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43710 AT2G43710
fragaria_vesca FvH4_4g10110 FvH4_4g10110
malus_domestica MD04G1048200.v1.1
prunus_persica Prupe.1G203700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0405901
rosa_laevigata RLG00000008819
rosa_multiflora Rmu_sc0001200.1_g000011
rosa_roxburghii Rroxscaffold_5G00350440
rosa_rugosa Rorug04G0062600
rosa_samantha Rh4AG134100 Rh4BG129200 Rh4CG142000 Rh4DG128500
rosa_wichuraiana Rw4G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1050
Acc16I TGCGCA 1 cut(s) 193
AccB1I GGYRCC 1 cut(s) 1279
AccI GTMKAC 1 cut(s) 26
AciI CCGC 2 cut(s) 172, 1079
AclWI GGATC 2 cut(s) 343, 554
AcoI YGGCCR 1 cut(s) 653
AcsI RAATTY 2 cut(s) 1168, 1296
AcuI CTGAAG 1 cut(s) 1022
AcyI GRCGYC 1 cut(s) 1280
AfaI GTAC 5 cut(s) 418, 445, 704, 797, 930
AfiI CCNNNNNNNGG 3 cut(s) 171, 227, 413
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 8 cut(s) 127, 272, 292, 586, 860, 1085, 1143, 1173
AhlI ACTAGT 1 cut(s) 851
AjnI CCWGG 2 cut(s) 232, 993
AjuI GAANNNNNNNTTGG 2 cut(s) 1303, 1335
AluBI AGCT 5 cut(s) 64, 391, 470, 813, 826
AluI AGCT 5 cut(s) 64, 391, 470, 813, 826
Alw26I GTCTC 1 cut(s) 1025
AlwI GGATC 2 cut(s) 343, 554
AoxI GGCC 1 cut(s) 653
ApeKI GCWGC 3 cut(s) 155, 352, 681
ApoI RAATTY 2 cut(s) 1168, 1296
ArsI GACNNNNNNTTYG 2 cut(s) 516, 548
AseI ATTAAT 1 cut(s) 1133
AspLEI GCGC 2 cut(s) 194, 1282
AspS9I GGNCC 3 cut(s) 57, 230, 997
AsuC2I CCSGG 1 cut(s) 726
AvaII GGWCC 3 cut(s) 57, 230, 997
AxyI CCTNAGG 1 cut(s) 306
BanI GGYRCC 1 cut(s) 1279
BanII GRGCYC 1 cut(s) 1208
BauI CACGAG 1 cut(s) 511
BbvI GCAGC 3 cut(s) 167, 339, 693
BccI CCATC 4 cut(s) 173, 221, 1065, 1069
BceAI ACGGC 1 cut(s) 668
BciT130I CCWGG 2 cut(s) 234, 995
BclI TGATCA 3 cut(s) 241, 340, 1162
BcnI CCSGG 1 cut(s) 726
BcoDI GTCTC 1 cut(s) 1025
BcuI ACTAGT 1 cut(s) 851
BfaI CTAG 1 cut(s) 852
BfmI CTRYAG 3 cut(s) 756, 949, 1213
BfoI RGCGCY 1 cut(s) 1283
BisI GCNGC 3 cut(s) 156, 353, 682
BlsI GCNGC 3 cut(s) 157, 354, 683
BmcAI AGTACT 2 cut(s) 445, 930
Bme1390I CCNGG 3 cut(s) 234, 726, 995
Bme18I GGWCC 3 cut(s) 57, 230, 997
BmgT120I GGNCC 3 cut(s) 57, 230, 997
BmiI GGNNCC 2 cut(s) 212, 1281
BmrFI CCNGG 3 cut(s) 234, 726, 995
BmsI GCATC 2 cut(s) 904, 926
BpmI CTGGAG 2 cut(s) 5, 1137
BpuMI CCSGG 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 1280
BsaJI CCNNGG 2 cut(s) 206, 331
BsaWI WCCGGW 1 cut(s) 1030
BsaXI ACNNNNNCTCC 4 cut(s) 195, 225, 414, 444
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 227, 413
Bse1I ACTGG 4 cut(s) 22, 59, 68, 1281
Bse21I CCTNAGG 1 cut(s) 306
BseBI CCWGG 2 cut(s) 234, 995
BseDI CCNNGG 2 cut(s) 206, 331
BseGI GGATG 3 cut(s) 919, 1039, 1080
BseLI CCNNNNNNNGG 3 cut(s) 171, 227, 413
BseMII CTCAG 3 cut(s) 297, 454, 1209
BseNI ACTGG 4 cut(s) 22, 59, 68, 1281
BseRI GAGGAG 1 cut(s) 203
BseXI GCAGC 3 cut(s) 167, 339, 693
Bsh1285I CGRYCG 1 cut(s) 581
BshFI GGCC 1 cut(s) 655
BshNI GGYRCC 1 cut(s) 1279
BsiEI CGRYCG 1 cut(s) 581
BsiSI CCGG 2 cut(s) 725, 1031
BslI CCNNNNNNNGG 3 cut(s) 171, 227, 413
BsmAI GTCTC 1 cut(s) 1025
BsmI GAATGC 1 cut(s) 743
BsnI GGCC 1 cut(s) 655
Bsp1286I GDGCHC 1 cut(s) 1208
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
Bsp19I CCATGG 1 cut(s) 206
BspACI CCGC 2 cut(s) 172, 1079
BspANI GGCC 1 cut(s) 655
BspCNI CTCAG 3 cut(s) 298, 453, 1208
BspHI TCATGA 3 cut(s) 238, 546, 1023
BspLI GGNNCC 2 cut(s) 212, 1281
BspMAI CTGCAG 2 cut(s) 760, 1217
BspPI GGATC 2 cut(s) 343, 554
BspT107I GGYRCC 1 cut(s) 1279
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 4 cut(s) 22, 59, 68, 1281
BssECI CCNNGG 2 cut(s) 206, 331
BssMI GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
BssNI GRCGYC 1 cut(s) 1280
BssSI CACGAG 1 cut(s) 511
BssT1I CCWWGG 1 cut(s) 206
Bst2BI CACGAG 1 cut(s) 511
Bst2UI CCWGG 2 cut(s) 234, 995
Bst4CI ACNGT 4 cut(s) 248, 266, 953, 1125
Bst6I CTCTTC 1 cut(s) 995
BstACI GRCGYC 1 cut(s) 1280
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 1 cut(s) 943
BstDEI CTNAG 4 cut(s) 35, 306, 440, 1195
BstDSI CCRYGG 1 cut(s) 206
BstF5I GGATG 3 cut(s) 919, 1039, 1080
BstH2I RGCGCY 1 cut(s) 1283
BstHHI GCGC 2 cut(s) 194, 1282
BstKTI GATC 8 cut(s) 244, 338, 343, 562, 581, 602, 988, 1165
BstMAI GTCTC 1 cut(s) 1025
BstMBI GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
BstMCI CGRYCG 1 cut(s) 581
BstMWI GCNNNNNNNGC 1 cut(s) 1212
BstNI CCWGG 2 cut(s) 234, 995
BstNSI RCATGY 2 cut(s) 304, 496
BstSCI CCNGG 3 cut(s) 232, 724, 993
BstSFI CTRYAG 3 cut(s) 756, 949, 1213
BstV1I GCAGC 3 cut(s) 167, 339, 693
BstX2I RGATCY 1 cut(s) 335
BstYI RGATCY 1 cut(s) 335
Bsu36I CCTNAGG 1 cut(s) 306
BsuRI GGCC 1 cut(s) 655
BtgI CCRYGG 1 cut(s) 206
BtsCI GGATG 3 cut(s) 919, 1039, 1080
BtsIMutI CAGTG 1 cut(s) 253
Cac8I GCNNGC 1 cut(s) 943
CciI TCATGA 3 cut(s) 238, 546, 1023
CfoI GCGC 2 cut(s) 194, 1282
Cfr13I GGNCC 3 cut(s) 57, 230, 997
Csp6I GTAC 5 cut(s) 417, 444, 703, 796, 929
CviQI GTAC 5 cut(s) 417, 444, 703, 796, 929
DdeI CTNAG 4 cut(s) 35, 306, 440, 1195
DinI GGCGCC 1 cut(s) 1281
DpnI GATC 8 cut(s) 243, 337, 342, 561, 580, 601, 987, 1164
DpnII GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
DraI TTTAAA 1 cut(s) 616
EaeI YGGCCR 1 cut(s) 653
Eam1104I CTCTTC 1 cut(s) 995
EarI CTCTTC 1 cut(s) 995
Eco130I CCWWGG 1 cut(s) 206
Eco24I GRGCYC 1 cut(s) 1208
Eco32I GATATC 1 cut(s) 487
Eco47I GGWCC 3 cut(s) 57, 230, 997
Eco57I CTGAAG 1 cut(s) 1022
Eco81I CCTNAGG 1 cut(s) 306
EcoRI GAATTC 1 cut(s) 1168
EcoRII CCWGG 2 cut(s) 232, 993
EcoRV GATATC 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 206
EcoT22I ATGCAT 1 cut(s) 919
EcoT38I GRGCYC 1 cut(s) 1208
EgeI GGCGCC 1 cut(s) 1281
EheI GGCGCC 1 cut(s) 1281
ErhI CCWWGG 1 cut(s) 206
FbaI TGATCA 3 cut(s) 241, 340, 1162
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 3 cut(s) 156, 353, 682
FokI GGATG 3 cut(s) 926, 1046, 1087
FriOI GRGCYC 1 cut(s) 1208
Fsp4HI GCNGC 3 cut(s) 156, 353, 682
FspBI CTAG 1 cut(s) 852
FspI TGCGCA 1 cut(s) 193
GlaI GCGC 2 cut(s) 193, 1281
GluI GCNGC 3 cut(s) 156, 353, 682
GsuI CTGGAG 2 cut(s) 5, 1137
HaeII RGCGCY 1 cut(s) 1283
HaeIII GGCC 1 cut(s) 655
HapII CCGG 2 cut(s) 725, 1031
HhaI GCGC 2 cut(s) 194, 1282
Hin1I GRCGYC 1 cut(s) 1280
Hin6I GCGC 2 cut(s) 192, 1280
HinP1I GCGC 2 cut(s) 192, 1280
HincII GTYRAC 1 cut(s) 73
HindII GTYRAC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 7 cut(s) 123, 161, 509, 708, 1058, 1085, 1148
HpaII CCGG 2 cut(s) 725, 1031
Hpy166II GTNNAC 4 cut(s) 27, 73, 419, 1274
Hpy188I TCNGA 5 cut(s) 61, 335, 340, 1167, 1198
Hpy188III TCNNGA 8 cut(s) 239, 452, 547, 557, 874, 1000, 1024, 1154
Hpy8I GTNNAC 4 cut(s) 27, 73, 419, 1274
HpyAV CCTTC 4 cut(s) 150, 227, 266, 1218
HpyCH4III ACNGT 4 cut(s) 248, 266, 953, 1125
HpyCH4V TGCA 5 cut(s) 370, 743, 758, 917, 1215
HpyF10VI GCNNNNNNNGC 1 cut(s) 1212
HpyF3I CTNAG 4 cut(s) 35, 306, 440, 1195
Hsp92I GRCGYC 1 cut(s) 1280
HspAI GCGC 2 cut(s) 192, 1280
KasI GGCGCC 1 cut(s) 1279
Ksp22I TGATCA 3 cut(s) 241, 340, 1162
Kzo9I GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
LmnI GCTCC 1 cut(s) 216
Lsp1109I GCAGC 3 cut(s) 167, 339, 693
LweI GCATC 2 cut(s) 904, 926
MaeI CTAG 1 cut(s) 852
MaeIII GTNAC 2 cut(s) 751, 845
MalI GATC 8 cut(s) 243, 337, 342, 561, 580, 601, 987, 1164
MboI GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
MboII GAAGA 4 cut(s) 350, 982, 1131, 1294
MflI RGATCY 1 cut(s) 335
MhlI GDGCHC 1 cut(s) 1208
MluCI AATT 9 cut(s) 98, 311, 586, 657, 896, 935, 1134, 1168, 1296
Mly113I GGCGCC 1 cut(s) 1280
MlyI GAGTC 1 cut(s) 503
MmeI TCCRAC 1 cut(s) 1217
MnlI CCTC 5 cut(s) 29, 224, 301, 341, 447
Mph1103I ATGCAT 1 cut(s) 919
MseI TTAA 5 cut(s) 402, 615, 629, 891, 1133
MslI CAYNNNNRTG 1 cut(s) 689
MspI CCGG 2 cut(s) 725, 1031
MspR9I CCNGG 3 cut(s) 234, 726, 995
Mva1269I GAATGC 1 cut(s) 743
MvaI CCWGG 2 cut(s) 234, 995
MwoI GCNNNNNNNGC 1 cut(s) 1212
NarI GGCGCC 1 cut(s) 1280
NciI CCSGG 1 cut(s) 726
NcoI CCATGG 1 cut(s) 206
NdeII GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
NlaIV GGNNCC 2 cut(s) 212, 1281
NmuCI GTSAC 1 cut(s) 751
NsbI TGCGCA 1 cut(s) 193
NsiI ATGCAT 1 cut(s) 919
NspI RCATGY 2 cut(s) 304, 496
PagI TCATGA 3 cut(s) 238, 546, 1023
PciI ACATGT 1 cut(s) 492
PctI GAATGC 1 cut(s) 743
PfeI GAWTC 6 cut(s) 123, 161, 708, 1058, 1085, 1148
PkrI GCNGC 3 cut(s) 157, 354, 683
Ple19I CGATCG 1 cut(s) 581
PleI GAGTC 1 cut(s) 503
PluTI GGCGCC 1 cut(s) 1283
PpsI GAGTC 1 cut(s) 503
PscI ACATGT 1 cut(s) 492
PshBI ATTAAT 1 cut(s) 1133
PsiI TTATAA 1 cut(s) 1050
Psp6I CCWGG 2 cut(s) 232, 993
PspGI CCWGG 2 cut(s) 232, 993
PspN4I GGNNCC 2 cut(s) 212, 1281
PspPI GGNCC 3 cut(s) 57, 230, 997
PstI CTGCAG 2 cut(s) 760, 1217
PsuI RGATCY 1 cut(s) 335
PvuI CGATCG 1 cut(s) 581
RsaI GTAC 5 cut(s) 418, 445, 704, 797, 930
RsaNI GTAC 5 cut(s) 417, 444, 703, 796, 929
RseI CAYNNNNRTG 1 cut(s) 689
SaqAI TTAA 5 cut(s) 402, 615, 629, 891, 1133
SatI GCNGC 3 cut(s) 156, 353, 682
Sau3AI GATC 8 cut(s) 241, 335, 340, 559, 578, 599, 985, 1162
Sau96I GGNCC 3 cut(s) 57, 230, 997
ScaI AGTACT 2 cut(s) 445, 930
SchI GAGTC 1 cut(s) 503
ScrFI CCNGG 3 cut(s) 234, 726, 995
SduI GDGCHC 1 cut(s) 1208
SetI ASST 7 cut(s) 66, 393, 439, 472, 815, 828, 1228
SfaNI GCATC 2 cut(s) 904, 926
SfcI CTRYAG 3 cut(s) 756, 949, 1213
SfoI GGCGCC 1 cut(s) 1281
SinI GGWCC 3 cut(s) 57, 230, 997
SmiMI CAYNNNNRTG 1 cut(s) 689
SpeI ACTAGT 1 cut(s) 851
Sse9I AATT 9 cut(s) 98, 311, 586, 657, 896, 935, 1134, 1168, 1296
SsiI CCGC 2 cut(s) 172, 1079
SspDI GGCGCC 1 cut(s) 1279
SspMI CTAG 1 cut(s) 852
StyD4I CCNGG 3 cut(s) 232, 724, 993
StyI CCWWGG 1 cut(s) 206
TaaI ACNGT 4 cut(s) 248, 266, 953, 1125
TaqI TCGA 2 cut(s) 528, 1330
TasI AATT 9 cut(s) 98, 311, 586, 657, 896, 935, 1134, 1168, 1296
TatI WGTACW 3 cut(s) 443, 702, 928
TfiI GAWTC 6 cut(s) 123, 161, 708, 1058, 1085, 1148
Tru1I TTAA 5 cut(s) 402, 615, 629, 891, 1133
Tru9I TTAA 5 cut(s) 402, 615, 629, 891, 1133
TscAI CASTG 1 cut(s) 253
TseFI GTSAC 1 cut(s) 751
TseI GCWGC 3 cut(s) 155, 352, 681
Tsp45I GTSAC 1 cut(s) 751
TspDTI ATGAA 6 cut(s) 132, 392, 620, 798, 952, 1040
TspRI CASTG 1 cut(s) 253
VpaK11BI GGWCC 3 cut(s) 57, 230, 997
VspI ATTAAT 1 cut(s) 1133
XapI RAATTY 2 cut(s) 1168, 1296
XceI RCATGY 2 cut(s) 304, 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 230
XmiI GTMKAC 1 cut(s) 26
XspI CTAG 1 cut(s) 852
ZrmI AGTACT 2 cut(s) 445, 930
Zsp2I ATGCAT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.