Rh4DG128500

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0014984
Physical Location & Seq
Reverse (-)
21275156 .. 21279134
3979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG128500.1

Sequence Viewer

Length: 1182 bp
ATGGCTCTCAAACTCAACGCCTTCACTATTCAATCCCCCAGGTTTGCTCTTCCCCAGATGGCCAGCCTCAGATCTCCCAAGTTCTCGATGGCCTCCACTCTCCGCTCTGGCGCCAAGGAGGTTGAGAATCTGAAGAAGCCTTTTACGCCTCCTCGGGAGGTGCATGTTCAAGTAACCCATTCCATGCCACCTCAAAAGATTGAGATCTTCAAATCCATTGAGGGTTGGGCTGAGGAGAACATCTTAGTTCACCTAAAGCCAGTTGAAAAGTGCTGGCAACCCCAGGATTTTTTGCCAGATCCTGCATCTGATGGATTTGAGGAACAAGTTAAGGAACTACGGGAGAGGGCAAAGGAGATTCCAGATGAATACTTTGTTGTTTTGGTCGGAGATATGATTACTGAAGAAGCCCTTCCAACTTATCAAACAATGCTTAATACCTTGGATGGAGTTAGGGATGAAACAGGTGCAAGCCCAACTCCTTGGGCAGTTTGGACAAGGGCATGGACAGCTGAAGAGAACAGGCACGGTGACCTTCTCAATAAGTATCTCTACCTCTCTGGCCGAGTAGACATGAGGCAAATTGAAAAGACGATTCAGTATTTGATTGGGTCTGGAATGGATCCGCGTACGGAGAACAGTCCTTACCTTGGGTTCATTTATACATCATTCCAGGAGAGGGCTACCTTCATTTCTCATGGAAATACTGCCAGGCATGCCAAGGAGTACGGGGATTTGAAGTTGGCTCAAATATGTGGTACAATAGCCTCAGATGAAAAGCGCCATGAGACTGCATATACTAAGATTGTGGAAAAGCTCTTTGAGATTGATCCCGATGGAACTGTCACGTCTTTTGCTGACATGATGAAGAAGAAGATCGCTATGCCAGCTCACTTGATGTATGATGGCCATGATGACAAACTTTTTGAGCACTTCTCAGCTGTTGCACAGCGTCTTGGTGTCTATACGGCCAAGGACTATGCAGATATATTGGAGTTCTTGGTGGGCAGGTGGAAGGTGCAGGACCTGACTGGACTTTCAGGTGAGGGTCGCAAGGCTCAAGAGTATCTGTGTGGGTTAGCCCCAAGAATTAGAAGACTAGAGGACAGAGCTCAAGCAAGGGCCAAGCAAGGACCTGCCATACCCTTCAGTTGGATATTTGATAGAAATGTGGGACTGTGA

Protein Analysis

393

Amino Acids

44.73

Weight (kDa)

6.11

Isoelectric Point (pI)

40.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 66 - 387 5.4e-161 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43710 AT2G43710
fragaria_vesca FvH4_4g10110 FvH4_4g10110
malus_domestica MD04G1048200.v1.1
prunus_persica Prupe.1G203700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0405901
rosa_laevigata RLG00000008819
rosa_multiflora Rmu_sc0001200.1_g000011
rosa_roxburghii Rroxscaffold_5G00350440
rosa_rugosa Rorug04G0062600
rosa_samantha Rh4AG134100 Rh4BG129200 Rh4CG142000 Rh4DG128500
rosa_wichuraiana Rw4G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 999
Acc36I ACCTGC 2 cut(s) 999, 1144
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 105
AccI GTMKAC 1 cut(s) 570
AccII CGCG 1 cut(s) 628
AciI CCGC 2 cut(s) 103, 626
AclWI GGATC 4 cut(s) 293, 617, 630, 824
AcoI YGGCCR 4 cut(s) 60, 562, 907, 969
AcuI CTGAAG 4 cut(s) 152, 423, 534, 1132
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 3 cut(s) 631, 728, 760
AfiI CCNNNNNNNGG 3 cut(s) 650, 679, 1152
AgsI TTSAA 6 cut(s) 32, 170, 211, 266, 587, 739
AjiI CACGTC 1 cut(s) 849
AjnI CCWGG 4 cut(s) 38, 282, 672, 710
AluBI AGCT 5 cut(s) 512, 817, 890, 941, 1112
AluI AGCT 5 cut(s) 512, 817, 890, 941, 1112
Alw21I GWGCWC 2 cut(s) 933, 1114
Alw26I GTCTC 1 cut(s) 782
AlwI GGATC 4 cut(s) 293, 617, 630, 824
AlwNI CAGNNNCTG 2 cut(s) 302, 1027
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 6 cut(s) 60, 90, 562, 907, 969, 1122
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
Asp700I GAANNNNTTC 1 cut(s) 411
AspLEI GCGC 2 cut(s) 113, 783
AspS9I GGNCC 3 cut(s) 1024, 1122, 1133
AsuHPI GGTGA 3 cut(s) 242, 542, 1055
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 1024, 1133
BalI TGGCCA 2 cut(s) 62, 909
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 1114
BbsI GAAGAC 1 cut(s) 1102
Bbv12I GWGCWC 2 cut(s) 933, 1114
BbvCI CCTCAGC 1 cut(s) 231
BccI CCATC 6 cut(s) 52, 82, 305, 440, 830, 899
BceAI ACGGC 1 cut(s) 984
BciT130I CCWGG 4 cut(s) 40, 284, 674, 712
BcoDI GTCTC 1 cut(s) 782
BfaI CTAG 1 cut(s) 1100
BfoI RGCGCY 2 cut(s) 114, 784
BfuAI ACCTGC 2 cut(s) 999, 1144
BglII AGATCT 2 cut(s) 71, 204
Bme1390I CCNGG 4 cut(s) 40, 284, 674, 712
Bme18I GGWCC 2 cut(s) 1024, 1133
BmeT110I CYCGRG 1 cut(s) 153
BmgBI CACGTC 1 cut(s) 849
BmgT120I GGNCC 3 cut(s) 1024, 1122, 1133
BmiI GGNNCC 2 cut(s) 112, 624
BmrFI CCNGG 4 cut(s) 40, 284, 674, 712
BmsI GCATC 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 1102
BplI GAGNNNNNCTC 2 cut(s) 920, 952
Bpu10I CCTNAGC 1 cut(s) 231
BpuEI CTTGAG 2 cut(s) 1044, 1098
BsaBI GATNNNNATC 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 9 cut(s) 38, 114, 152, 282, 441, 482, 649, 720, 972
Bsc4I CCNNNNNNNGG 3 cut(s) 650, 679, 1152
Bse1I ACTGG 2 cut(s) 260, 1036
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 4 cut(s) 40, 284, 674, 712
BseDI CCNNGG 9 cut(s) 38, 114, 152, 282, 441, 482, 649, 720, 972
BseGI GGATG 2 cut(s) 451, 463
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 3 cut(s) 650, 679, 1152
BseMII CTCAG 4 cut(s) 82, 222, 783, 951
BseNI ACTGG 2 cut(s) 260, 1036
BseRI GAGGAG 2 cut(s) 141, 248
BsgI GTGCAG 1 cut(s) 1040
Bsh1236I CGCG 1 cut(s) 628
BshFI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BshNI GGYRCC 1 cut(s) 110
BsiHKAI GWGCWC 2 cut(s) 933, 1114
BsiHKCI CYCGRG 1 cut(s) 153
BsiWI CGTACG 1 cut(s) 629
BslI CCNNNNNNNGG 3 cut(s) 650, 679, 1152
BsmAI GTCTC 1 cut(s) 782
BsnI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 2 cut(s) 933, 1114
Bsp143I GATC 6 cut(s) 71, 204, 298, 622, 829, 876
BspACI CCGC 2 cut(s) 103, 626
BspANI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BspCNI CTCAG 4 cut(s) 81, 223, 782, 950
BspFNI CGCG 1 cut(s) 628
BspLI GGNNCC 2 cut(s) 112, 624
BspMI ACCTGC 2 cut(s) 999, 1144
BspPI GGATC 4 cut(s) 293, 617, 630, 824
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 105
BsrI ACTGG 2 cut(s) 260, 1036
BssECI CCNNGG 9 cut(s) 38, 114, 152, 282, 441, 482, 649, 720, 972
BssMI GATC 6 cut(s) 71, 204, 298, 622, 829, 876
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 6 cut(s) 114, 441, 482, 649, 720, 972
Bst2UI CCWGG 4 cut(s) 40, 284, 674, 712
Bst4CI ACNGT 4 cut(s) 530, 641, 844, 1179
Bst6I CTCTTC 2 cut(s) 54, 510
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 5 cut(s) 64, 275, 472, 717, 888
BstDEI CTNAG 6 cut(s) 68, 231, 244, 769, 801, 937
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 2 cut(s) 451, 463
BstFNI CGCG 1 cut(s) 628
BstH2I RGCGCY 2 cut(s) 114, 784
BstHHI GCGC 2 cut(s) 113, 783
BstKTI GATC 6 cut(s) 74, 207, 301, 625, 832, 879
BstMAI GTCTC 1 cut(s) 782
BstMBI GATC 6 cut(s) 71, 204, 298, 622, 829, 876
BstMWI GCNNNNNNNGC 4 cut(s) 145, 509, 716, 887
BstNI CCWGG 4 cut(s) 40, 284, 674, 712
BstNSI RCATGY 2 cut(s) 167, 719
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 4 cut(s) 38, 282, 672, 710
BstUI CGCG 1 cut(s) 628
BstV2I GAAGAC 1 cut(s) 1102
BstX2I RGATCY 4 cut(s) 71, 204, 298, 622
BstXI CCANNNNNNTGG 1 cut(s) 483
BstYI RGATCY 4 cut(s) 71, 204, 298, 622
BsuRI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BtrI CACGTC 1 cut(s) 849
BtsCI GGATG 2 cut(s) 451, 463
BveI ACCTGC 2 cut(s) 999, 1144
Cac8I GCNNGC 5 cut(s) 64, 275, 472, 717, 888
CaiI CAGNNNCTG 2 cut(s) 302, 1027
CfoI GCGC 2 cut(s) 113, 783
Cfr13I GGNCC 3 cut(s) 1024, 1122, 1133
CseI GACGC 1 cut(s) 941
Csp6I GTAC 3 cut(s) 630, 727, 759
CviAII CATG 9 cut(s) 164, 184, 504, 574, 698, 716, 785, 862, 911
CviQI GTAC 3 cut(s) 630, 727, 759
DdeI CTNAG 6 cut(s) 68, 231, 244, 769, 801, 937
DinI GGCGCC 1 cut(s) 112
DpnI GATC 6 cut(s) 73, 206, 300, 624, 831, 878
DpnII GATC 6 cut(s) 71, 204, 298, 622, 829, 876
EaeI YGGCCR 4 cut(s) 60, 562, 907, 969
Eam1104I CTCTTC 2 cut(s) 54, 510
EarI CTCTTC 2 cut(s) 54, 510
Ecl136II GAGCTC 1 cut(s) 1112
Eco130I CCWWGG 6 cut(s) 114, 441, 482, 649, 720, 972
Eco24I GRGCYC 1 cut(s) 1114
Eco47I GGWCC 2 cut(s) 1024, 1133
Eco53kI GAGCTC 1 cut(s) 1112
Eco57I CTGAAG 4 cut(s) 152, 423, 534, 1132
Eco88I CYCGRG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 530
EcoICRI GAGCTC 1 cut(s) 1112
EcoO109I RGGNCCY 2 cut(s) 1024, 1133
EcoO65I GGTNACC 1 cut(s) 530
EcoRII CCWGG 4 cut(s) 38, 282, 672, 710
EcoT14I CCWWGG 6 cut(s) 114, 441, 482, 649, 720, 972
EcoT38I GRGCYC 1 cut(s) 1114
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 6 cut(s) 114, 441, 482, 649, 720, 972
FaeI CATG 9 cut(s) 167, 187, 507, 577, 701, 719, 788, 865, 914
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FatI CATG 9 cut(s) 163, 183, 503, 573, 697, 715, 784, 861, 910
FblI GTMKAC 1 cut(s) 570
FokI GGATG 2 cut(s) 458, 470
FriOI GRGCYC 1 cut(s) 1114
FspBI CTAG 1 cut(s) 1100
GlaI GCGC 2 cut(s) 112, 782
HaeII RGCGCY 2 cut(s) 114, 784
HaeIII GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
HgaI GACGC 1 cut(s) 941
HhaI GCGC 2 cut(s) 113, 783
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 9 cut(s) 167, 187, 507, 577, 701, 719, 788, 865, 914
Hin6I GCGC 2 cut(s) 111, 781
HinP1I GCGC 2 cut(s) 111, 781
HinfI GANTC 3 cut(s) 127, 358, 595
HphI GGTGA 3 cut(s) 242, 542, 1055
Hpy166II GTNNAC 2 cut(s) 250, 571
Hpy188I TCNGA 5 cut(s) 71, 132, 310, 389, 772
Hpy188III TCNNGA 6 cut(s) 85, 155, 362, 615, 833, 1061
Hpy8I GTNNAC 2 cut(s) 250, 571
HpyAV CCTTC 6 cut(s) 31, 422, 545, 697, 1009, 1156
HpyCH4III ACNGT 4 cut(s) 530, 641, 844, 1179
HpyCH4IV ACGT 1 cut(s) 848
HpyCH4V TGCA 7 cut(s) 163, 305, 470, 794, 947, 983, 1021
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 509, 716, 887
HpyF3I CTNAG 6 cut(s) 68, 231, 244, 769, 801, 937
HpySE526I ACGT 1 cut(s) 848
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 9 cut(s) 167, 187, 507, 577, 701, 719, 788, 865, 914
HspAI GCGC 2 cut(s) 111, 781
KasI GGCGCC 1 cut(s) 110
Kzo9I GATC 6 cut(s) 71, 204, 298, 622, 829, 876
LguI GCTCTTC 1 cut(s) 54
LweI GCATC 1 cut(s) 314
MaeI CTAG 1 cut(s) 1100
MaeII ACGT 1 cut(s) 848
MaeIII GTNAC 3 cut(s) 172, 530, 844
MalI GATC 6 cut(s) 73, 206, 300, 624, 831, 878
MbiI CCGCTC 1 cut(s) 105
MboI GATC 6 cut(s) 71, 204, 298, 622, 829, 876
MboII GAAGA 9 cut(s) 41, 145, 199, 416, 527, 880, 883, 886, 1107
MflI RGATCY 4 cut(s) 71, 204, 298, 622
MhlI GDGCHC 2 cut(s) 933, 1114
MlsI TGGCCA 2 cut(s) 62, 909
MluCI AATT 2 cut(s) 582, 1089
MluNI TGGCCA 2 cut(s) 62, 909
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 3 cut(s) 367, 440, 1133
Mox20I TGGCCA 2 cut(s) 62, 909
MroXI GAANNNNTTC 1 cut(s) 411
MscI TGGCCA 2 cut(s) 62, 909
MseI TTAA 2 cut(s) 330, 435
Msp20I TGGCCA 2 cut(s) 62, 909
MspA1I CMGCKG 2 cut(s) 512, 941
MspR9I CCNGG 4 cut(s) 40, 284, 674, 712
MvaI CCWGG 4 cut(s) 40, 284, 674, 712
MvnI CGCG 1 cut(s) 628
MwoI GCNNNNNNNGC 4 cut(s) 145, 509, 716, 887
NarI GGCGCC 1 cut(s) 111
NdeII GATC 6 cut(s) 71, 204, 298, 622, 829, 876
NlaIII CATG 9 cut(s) 167, 187, 507, 577, 701, 719, 788, 865, 914
NlaIV GGNNCC 2 cut(s) 112, 624
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 2 cut(s) 530, 844
NspI RCATGY 2 cut(s) 167, 719
PaeI GCATGC 1 cut(s) 719
PaqCI CACCTGC 1 cut(s) 999
PciSI GCTCTTC 1 cut(s) 54
PdmI GAANNNNTTC 1 cut(s) 411
PfeI GAWTC 3 cut(s) 127, 358, 595
Pfl23II CGTACG 1 cut(s) 629
PfoI TCCNGGA 1 cut(s) 672
PluTI GGCGCC 1 cut(s) 114
PpuMI RGGWCCY 2 cut(s) 1024, 1133
Psp124BI GAGCTC 1 cut(s) 1114
Psp5II RGGWCCY 2 cut(s) 1024, 1133
Psp6I CCWGG 4 cut(s) 38, 282, 672, 710
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 4 cut(s) 38, 282, 672, 710
PspLI CGTACG 1 cut(s) 629
PspN4I GGNNCC 2 cut(s) 112, 624
PspPI GGNCC 3 cut(s) 1024, 1122, 1133
PspPPI RGGWCCY 2 cut(s) 1024, 1133
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PstNI CAGNNNCTG 2 cut(s) 302, 1027
PsuI RGATCY 4 cut(s) 71, 204, 298, 622
PvuII CAGCTG 2 cut(s) 512, 941
RsaI GTAC 3 cut(s) 631, 728, 760
RsaNI GTAC 3 cut(s) 630, 727, 759
SacI GAGCTC 1 cut(s) 1114
SapI GCTCTTC 1 cut(s) 54
SaqAI TTAA 2 cut(s) 330, 435
Sau3AI GATC 6 cut(s) 71, 204, 298, 622, 829, 876
Sau96I GGNCC 3 cut(s) 1024, 1122, 1133
ScrFI CCNGG 4 cut(s) 40, 284, 674, 712
SduI GDGCHC 2 cut(s) 933, 1114
SfaNI GCATC 1 cut(s) 314
SfoI GGCGCC 1 cut(s) 112
SinI GGWCC 2 cut(s) 1024, 1133
SmlI CTYRAG 2 cut(s) 1059, 1113
SmoI CTYRAG 2 cut(s) 1059, 1113
SphI GCATGC 1 cut(s) 719
Sse9I AATT 2 cut(s) 582, 1089
SsiI CCGC 2 cut(s) 103, 626
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 1 cut(s) 1100
SstI GAGCTC 1 cut(s) 1114
StyD4I CCNGG 4 cut(s) 38, 282, 672, 710
StyI CCWWGG 6 cut(s) 114, 441, 482, 649, 720, 972
TaaI ACNGT 4 cut(s) 530, 641, 844, 1179
TaiI ACGT 1 cut(s) 851
TaqI TCGA 1 cut(s) 86
TasI AATT 2 cut(s) 582, 1089
TfiI GAWTC 3 cut(s) 127, 358, 595
Tru1I TTAA 2 cut(s) 330, 435
Tru9I TTAA 2 cut(s) 330, 435
TseFI GTSAC 2 cut(s) 530, 844
Tsp45I GTSAC 2 cut(s) 530, 844
TspDTI ATGAA 6 cut(s) 381, 474, 646, 679, 789, 881
TspGWI ACGGA 1 cut(s) 647
VpaK11BI GGWCC 2 cut(s) 1024, 1133
XceI RCATGY 2 cut(s) 167, 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 411
XspI CTAG 1 cut(s) 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.