FvH4_4g10110

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014984
Physical Location & Seq
Reverse (-)
12943084 .. 12947580
4497 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g10110.t2

Sequence Viewer

Length: 1182 bp
ATGGCTCTCAAACTCAACGCCTTCCCTACTCAATCACCAAGGTTTGCTCTTCCGCAGATGGCCAGCCTCAGATCTCCCAAGTTCTCCATGGCCTCCACTCTCCGCTCTGGCGCCAAGGAGGTTGAGAATCTGAAGAAGCCTTTCACACCTCCTCGGGAGGTGCATGTTCAAGTAACCCATTCCATGCCACCTCAAAAGATTGAGATCTTCAAATCCATTGAGGGTTGGGCTGAACAGAACATCTTAGTTCACCTAAAGCCAGTTGAAAAGTGTTGGCAACCCCAGGATTTTTTGCCAGATCCTGCATCCGATGGATTTGAAGACCAAGTTAGGGAACTACGGGAGAGGGCAAAGGAGATTCCAGATGAATACTTTGTTGTTTTGGTTGGAGATATGATTACTGAAGAAGCCCTTCCAACTTATCAAACCATGCTAAATACCTTGGATGGAGTTCGGGATGAAACAGGTGCAAGCCCAACTCCTTGGGCAGTTTGGACAAGGGCATGGACAGCCGAAGAGAACAGGCATGGTGACCTTCTCAATAAGTACCTTTACCTCTGTGGCCGAGTAGATATGAGACAAATTGAAAAGACTATTCAGTATTTGATTGGGTCTGGCATGGATCCGCGTACGGAGAACAGTCCTTACCTTGGGTTCATTTATACATCGTTCCAGGAGAGGGCTACCTTCATCTCTCATGGAAACACTGCCAGGCATGCCAAGGACTATGGGGACTTGAAGTTGGCTCAAATATGTGGCACAATAGCCTCAGATGAGAAGCGCCATGAGACTGCATATACCAAGATTGTTGAAAAGCTCTTTGAGATTGATCCTGATGGAACTATCACATCTTTTGCTGACATGATGAAGAAGAAGATCGCTATGCCAGCTCACTTGATGTATGACGGCCGCGATGACAAACTTTTTGATCACTTTTCAGCTGTTGCACAGCGTCTTGGTGTCTATACGGCCAAGGACTATGCAGATATATTGGAGTTCTTGGTGGGCAGGTGGAAGGTGCAGGACCTGACTGGACTTTCAGGTGAGGGTCGCAAGGCTCAAGAATATCTGTGTGGGTTAGCCCCAAGAATTAGACGACTAGAGGAAAGAGCTCAAGCAAGGGCCAAGCAAGGTCCTGCCATACCTTTCAGTTGGATCTTTGATAGAAATGTGGGGCTGTGA

Protein Analysis

394

Amino Acids

44.77

Weight (kDa)

6.35

Isoelectric Point (pI)

40.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 5 - 326 1e-160 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014984)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G43710 AT2G43710
fragaria_vesca FvH4_4g10110 FvH4_4g10110
malus_domestica MD04G1048200.v1.1
prunus_persica Prupe.1G203700_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0405901
rosa_laevigata RLG00000008819
rosa_multiflora Rmu_sc0001200.1_g000011
rosa_roxburghii Rroxscaffold_5G00350440
rosa_rugosa Rorug04G0062600
rosa_samantha Rh4AG134100 Rh4BG129200 Rh4CG142000 Rh4DG128500
rosa_wichuraiana Rw4G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 999
Acc36I ACCTGC 1 cut(s) 999
AccB1I GGYRCC 1 cut(s) 110
AccBSI CCGCTC 1 cut(s) 105
AccII CGCG 2 cut(s) 628, 912
AciI CCGC 4 cut(s) 53, 103, 626, 910
AclWI GGATC 5 cut(s) 293, 617, 630, 824, 1163
AcoI YGGCCR 4 cut(s) 60, 562, 907, 969
AcuI CTGAAG 2 cut(s) 152, 423
AcyI GRCGYC 1 cut(s) 111
AfaI GTAC 2 cut(s) 548, 631
AfiI CCNNNNNNNGG 3 cut(s) 331, 650, 679
AgsI TTSAA 7 cut(s) 170, 211, 266, 320, 587, 739, 812
AjnI CCWGG 3 cut(s) 282, 672, 710
AluBI AGCT 4 cut(s) 817, 890, 941, 1112
AluI AGCT 4 cut(s) 817, 890, 941, 1112
Alw21I GWGCWC 1 cut(s) 1114
Alw26I GTCTC 2 cut(s) 571, 782
AlwI GGATC 5 cut(s) 293, 617, 630, 824, 1163
AlwNI CAGNNNCTG 2 cut(s) 302, 1027
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 6 cut(s) 60, 90, 562, 907, 969, 1122
ArsI GACNNNNNNTTYG 2 cut(s) 908, 940
Asp700I GAANNNNTTC 2 cut(s) 140, 411
AspLEI GCGC 2 cut(s) 113, 783
AspS9I GGNCC 3 cut(s) 1024, 1122, 1133
AsuHPI GGTGA 4 cut(s) 27, 242, 542, 1055
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 2 cut(s) 1024, 1133
BalI TGGCCA 1 cut(s) 62
BamHI GGATCC 1 cut(s) 622
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 1114
BbsI GAAGAC 1 cut(s) 327
Bbv12I GWGCWC 1 cut(s) 1114
BccI CCATC 4 cut(s) 52, 305, 440, 830
BceAI ACGGC 2 cut(s) 922, 984
BciT130I CCWGG 3 cut(s) 284, 674, 712
BclI TGATCA 1 cut(s) 928
BcoDI GTCTC 2 cut(s) 571, 782
BfaI CTAG 1 cut(s) 1100
BfoI RGCGCY 2 cut(s) 114, 784
BfuAI ACCTGC 1 cut(s) 999
BglII AGATCT 2 cut(s) 71, 204
BisI GCNGC 1 cut(s) 910
BlsI GCNGC 1 cut(s) 911
Bme1390I CCNGG 3 cut(s) 284, 674, 712
Bme18I GGWCC 2 cut(s) 1024, 1133
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 1024, 1122, 1133
BmiI GGNNCC 2 cut(s) 112, 624
BmrFI CCNGG 3 cut(s) 284, 674, 712
BmsI GCATC 1 cut(s) 314
BpiI GAAGAC 1 cut(s) 327
BpuEI CTTGAG 2 cut(s) 1044, 1098
BsaBI GATNNNNATC 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 111
Bsc4I CCNNNNNNNGG 3 cut(s) 331, 650, 679
Bse1I ACTGG 2 cut(s) 260, 1036
Bse8I GATNNNNATC 1 cut(s) 203
BseBI CCWGG 3 cut(s) 284, 674, 712
BseGI GGATG 3 cut(s) 305, 451, 463
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 3 cut(s) 331, 650, 679
BseMII CTCAG 2 cut(s) 82, 783
BseNI ACTGG 2 cut(s) 260, 1036
BseRI GAGGAG 1 cut(s) 141
BseX3I CGGCCG 1 cut(s) 907
BsgI GTGCAG 1 cut(s) 1040
Bsh1236I CGCG 2 cut(s) 628, 912
Bsh1285I CGRYCG 1 cut(s) 910
BshFI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BshNI GGYRCC 1 cut(s) 110
BsiEI CGRYCG 1 cut(s) 910
BsiHKAI GWGCWC 1 cut(s) 1114
BsiHKCI CYCGRG 1 cut(s) 153
BsiWI CGTACG 1 cut(s) 629
BslFI GGGAC 1 cut(s) 746
BslI CCNNNNNNNGG 3 cut(s) 331, 650, 679
BsmAI GTCTC 2 cut(s) 571, 782
BsmFI GGGAC 1 cut(s) 746
BsnI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BsoBI CYCGRG 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 1114
Bsp143I GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 4 cut(s) 53, 103, 626, 910
BspANI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BspCNI CTCAG 2 cut(s) 81, 782
BspFNI CGCG 2 cut(s) 628, 912
BspLI GGNNCC 2 cut(s) 112, 624
BspMI ACCTGC 1 cut(s) 999
BspPI GGATC 5 cut(s) 293, 617, 630, 824, 1163
BspQI GCTCTTC 1 cut(s) 54
BspT107I GGYRCC 1 cut(s) 110
BsrBI CCGCTC 1 cut(s) 105
BsrI ACTGG 2 cut(s) 260, 1036
BssMI GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 8 cut(s) 38, 87, 114, 441, 482, 649, 720, 972
Bst2UI CCWGG 3 cut(s) 284, 674, 712
Bst4CI ACNGT 1 cut(s) 641
Bst6I CTCTTC 2 cut(s) 54, 510
BstACI GRCGYC 1 cut(s) 111
BstC8I GCNNGC 4 cut(s) 64, 472, 717, 888
BstDEI CTNAG 3 cut(s) 68, 244, 769
BstDSI CCRYGG 1 cut(s) 87
BstEII GGTNACC 1 cut(s) 530
BstF5I GGATG 3 cut(s) 305, 451, 463
BstFNI CGCG 2 cut(s) 628, 912
BstH2I RGCGCY 2 cut(s) 114, 784
BstHHI GCGC 2 cut(s) 113, 783
BstKTI GATC 8 cut(s) 74, 207, 301, 625, 832, 879, 931, 1158
BstMAI GTCTC 2 cut(s) 571, 782
BstMBI GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
BstMCI CGRYCG 1 cut(s) 910
BstMWI GCNNNNNNNGC 3 cut(s) 509, 716, 887
BstNI CCWGG 3 cut(s) 284, 674, 712
BstNSI RCATGY 2 cut(s) 167, 719
BstPI GGTNACC 1 cut(s) 530
BstSCI CCNGG 3 cut(s) 282, 672, 710
BstUI CGCG 2 cut(s) 628, 912
BstV2I GAAGAC 1 cut(s) 327
BstX2I RGATCY 5 cut(s) 71, 204, 298, 622, 1155
BstXI CCANNNNNNTGG 1 cut(s) 483
BstYI RGATCY 5 cut(s) 71, 204, 298, 622, 1155
BstZI CGGCCG 1 cut(s) 907
BsuRI GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 927
BtsCI GGATG 3 cut(s) 305, 451, 463
BtsI GCAGTG 1 cut(s) 705
BtsIMutI CAGTG 1 cut(s) 705
BveI ACCTGC 1 cut(s) 999
Cac8I GCNNGC 4 cut(s) 64, 472, 717, 888
CaiI CAGNNNCTG 2 cut(s) 302, 1027
CfoI GCGC 2 cut(s) 113, 783
Cfr13I GGNCC 3 cut(s) 1024, 1122, 1133
CseI GACGC 1 cut(s) 941
Csp6I GTAC 2 cut(s) 547, 630
CviQI GTAC 2 cut(s) 547, 630
DdeI CTNAG 3 cut(s) 68, 244, 769
DinI GGCGCC 1 cut(s) 112
DpnI GATC 8 cut(s) 73, 206, 300, 624, 831, 878, 930, 1157
DpnII GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
EaeI YGGCCR 4 cut(s) 60, 562, 907, 969
EagI CGGCCG 1 cut(s) 907
Eam1104I CTCTTC 2 cut(s) 54, 510
EarI CTCTTC 2 cut(s) 54, 510
Ecl136II GAGCTC 1 cut(s) 1112
EclXI CGGCCG 1 cut(s) 907
Eco130I CCWWGG 8 cut(s) 38, 87, 114, 441, 482, 649, 720, 972
Eco24I GRGCYC 1 cut(s) 1114
Eco47I GGWCC 2 cut(s) 1024, 1133
Eco52I CGGCCG 1 cut(s) 907
Eco53kI GAGCTC 1 cut(s) 1112
Eco57I CTGAAG 2 cut(s) 152, 423
Eco88I CYCGRG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 530
EcoICRI GAGCTC 1 cut(s) 1112
EcoO109I RGGNCCY 2 cut(s) 1024, 1133
EcoO65I GGTNACC 1 cut(s) 530
EcoRII CCWGG 3 cut(s) 282, 672, 710
EcoT14I CCWWGG 8 cut(s) 38, 87, 114, 441, 482, 649, 720, 972
EcoT38I GRGCYC 1 cut(s) 1114
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 8 cut(s) 38, 87, 114, 441, 482, 649, 720, 972
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FaqI GGGAC 1 cut(s) 746
FbaI TGATCA 1 cut(s) 928
Fnu4HI GCNGC 1 cut(s) 910
FokI GGATG 3 cut(s) 292, 458, 470
FriOI GRGCYC 1 cut(s) 1114
Fsp4HI GCNGC 1 cut(s) 910
FspBI CTAG 1 cut(s) 1100
GlaI GCGC 2 cut(s) 112, 782
GluI GCNGC 1 cut(s) 910
HaeII RGCGCY 2 cut(s) 114, 784
HaeIII GGCC 6 cut(s) 62, 92, 564, 909, 971, 1124
HgaI GACGC 1 cut(s) 941
HhaI GCGC 2 cut(s) 113, 783
Hin1I GRCGYC 1 cut(s) 111
Hin6I GCGC 2 cut(s) 111, 781
HinP1I GCGC 2 cut(s) 111, 781
HinfI GANTC 2 cut(s) 127, 358
HphI GGTGA 4 cut(s) 27, 242, 542, 1055
Hpy166II GTNNAC 1 cut(s) 250
Hpy188I TCNGA 4 cut(s) 71, 132, 310, 772
Hpy188III TCNNGA 5 cut(s) 155, 362, 455, 833, 1061
Hpy8I GTNNAC 1 cut(s) 250
HpyAV CCTTC 5 cut(s) 31, 422, 545, 697, 1009
HpyCH4III ACNGT 1 cut(s) 641
HpyCH4V TGCA 7 cut(s) 163, 305, 470, 794, 947, 983, 1021
HpyF10VI GCNNNNNNNGC 3 cut(s) 509, 716, 887
HpyF3I CTNAG 3 cut(s) 68, 244, 769
Hsp92I GRCGYC 1 cut(s) 111
HspAI GCGC 2 cut(s) 111, 781
KasI GGCGCC 1 cut(s) 110
Ksp22I TGATCA 1 cut(s) 928
Kzo9I GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
LguI GCTCTTC 1 cut(s) 54
LweI GCATC 1 cut(s) 314
MaeI CTAG 1 cut(s) 1100
MaeIII GTNAC 2 cut(s) 172, 530
MalI GATC 8 cut(s) 73, 206, 300, 624, 831, 878, 930, 1157
MbiI CCGCTC 1 cut(s) 105
MboI GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
MboII GAAGA 9 cut(s) 41, 145, 199, 332, 416, 527, 880, 883, 886
MflI RGATCY 5 cut(s) 71, 204, 298, 622, 1155
MhlI GDGCHC 1 cut(s) 1114
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 2 cut(s) 582, 1089
MluNI TGGCCA 1 cut(s) 62
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 3 cut(s) 367, 440, 1133
Mox20I TGGCCA 1 cut(s) 62
MroXI GAANNNNTTC 2 cut(s) 140, 411
MscI TGGCCA 1 cut(s) 62
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 941
MspR9I CCNGG 3 cut(s) 284, 674, 712
MvaI CCWGG 3 cut(s) 284, 674, 712
MvnI CGCG 2 cut(s) 628, 912
MwoI GCNNNNNNNGC 3 cut(s) 509, 716, 887
NarI GGCGCC 1 cut(s) 111
NcoI CCATGG 1 cut(s) 87
NdeII GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
NlaIV GGNNCC 2 cut(s) 112, 624
NmeAIII GCCGAG 1 cut(s) 590
NmuCI GTSAC 1 cut(s) 530
NspI RCATGY 2 cut(s) 167, 719
PaeI GCATGC 1 cut(s) 719
PaqCI CACCTGC 1 cut(s) 999
PciSI GCTCTTC 1 cut(s) 54
PdmI GAANNNNTTC 2 cut(s) 140, 411
PfeI GAWTC 2 cut(s) 127, 358
Pfl23II CGTACG 1 cut(s) 629
PfoI TCCNGGA 1 cut(s) 672
PkrI GCNGC 1 cut(s) 911
PluTI GGCGCC 1 cut(s) 114
PpuMI RGGWCCY 2 cut(s) 1024, 1133
Psp124BI GAGCTC 1 cut(s) 1114
Psp5II RGGWCCY 2 cut(s) 1024, 1133
Psp6I CCWGG 3 cut(s) 282, 672, 710
PspEI GGTNACC 1 cut(s) 530
PspGI CCWGG 3 cut(s) 282, 672, 710
PspLI CGTACG 1 cut(s) 629
PspN4I GGNNCC 2 cut(s) 112, 624
PspPI GGNCC 3 cut(s) 1024, 1122, 1133
PspPPI RGGWCCY 2 cut(s) 1024, 1133
PsrI GAACNNNNNNTAC 2 cut(s) 629, 661
PstNI CAGNNNCTG 2 cut(s) 302, 1027
PsuI RGATCY 5 cut(s) 71, 204, 298, 622, 1155
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 2 cut(s) 548, 631
RsaNI GTAC 2 cut(s) 547, 630
SacI GAGCTC 1 cut(s) 1114
SapI GCTCTTC 1 cut(s) 54
SatI GCNGC 1 cut(s) 910
Sau3AI GATC 8 cut(s) 71, 204, 298, 622, 829, 876, 928, 1155
Sau96I GGNCC 3 cut(s) 1024, 1122, 1133
ScrFI CCNGG 3 cut(s) 284, 674, 712
SduI GDGCHC 1 cut(s) 1114
SfaNI GCATC 1 cut(s) 314
SfoI GGCGCC 1 cut(s) 112
SinI GGWCC 2 cut(s) 1024, 1133
SmlI CTYRAG 2 cut(s) 1059, 1113
SmoI CTYRAG 2 cut(s) 1059, 1113
SphI GCATGC 1 cut(s) 719
Sse9I AATT 2 cut(s) 582, 1089
SsiI CCGC 4 cut(s) 53, 103, 626, 910
SspDI GGCGCC 1 cut(s) 110
SspMI CTAG 1 cut(s) 1100
SstI GAGCTC 1 cut(s) 1114
StyD4I CCNGG 3 cut(s) 282, 672, 710
StyI CCWWGG 8 cut(s) 38, 87, 114, 441, 482, 649, 720, 972
TaaI ACNGT 1 cut(s) 641
TasI AATT 2 cut(s) 582, 1089
TauI GCSGC 1 cut(s) 912
TfiI GAWTC 2 cut(s) 127, 358
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 1 cut(s) 530
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 5 cut(s) 381, 474, 646, 679, 881
TspGWI ACGGA 1 cut(s) 647
TspRI CASTG 1 cut(s) 712
VpaK11BI GGWCC 2 cut(s) 1024, 1133
XceI RCATGY 2 cut(s) 167, 719
XcmI CCANNNNNNNNNTGG 1 cut(s) 85
XmnI GAANNNNTTC 2 cut(s) 140, 411
XspI CTAG 1 cut(s) 1100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.