Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014984

Physical Location & Seq

Genomic Coordinates Reverse (-)

5645106 .. 5646331

Gene Structure 1226 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1048200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1071 bp

ATGCATGCTTGCAGAGAAAGAGAGCTCAGGATTGATGAGATTAACATTAGTAATGGGAATTATGTACACAATTACAAGAAGGCTTTCATGCCTCAGCGCGAGTTGCGTTATCAAGAAATTACACATTCTATGCCAAGTCAAAAGATTGAGATATTCAAATACCTAGAGGACTGGGCTGAGCACAACATTTTGATTCACTTGAAACAAGTTGACAAGTGTTGGCCACCTCAAGATTTTCTTCCTCACTCTCGAACTACTGGTGATACGGATGGATTTCTTGAGCAAGTCAAGGAACTAAGGGAGAGGGCAAATGAGATTCCTGATGATTACTTTGTTGTTTTGGTCGGAAACATGATCACTGAAGACGCCCTTCCGACTTACCAAACGATGCTTAATACATTGGATGGAGTTCAAGATGAAACAGGTTCAAGCCCTATTTCTTGGAACAGGGCATGGACTGCTGAAGACAACAGGCATGGGGACCTTCTCAATAAGTATCTCTATCTCACTGGACGAGTCGACATGTCCCAAATTGAGAGGACCATTCAGTATCTGATTACTTATGGAATGGATCCCGGGGGTGAGAACAACCCTTACTTGGCCTTCATATATACTTCATTCCAGGAAAGGGCTACCGCTATCTCTCATGGGAACACTGCCAAACTTGCCAAGGAGCATGGGGACTTGAAGTTGGCGCAAATATGCGGTACAATAGCCTCCGATGAGAAGCGCCATGAGGCGGCCTACAAAAAGATACAAATCACTATGCCCGCTCACTTGATGTATGATGGTCGTGATCACCACCTTTTCCACCACTTTTCCAGTGTTGCGCAGCGCCTTGGATTATATACCACCAATGACTATGCCGATATACTGGAGTTGTTGCTCTCCGTGTGGAAGGTTGAAACCCTAACCGGACTTTCACCTGAGGGCCGAAAGGCTCAGGATTATGTTTGTGGGTTGGCCCTAAGAATAAGAAAACTGGAGGAAAGAGTTCAATTAAGGGACAAGCGACCACTCACCTCCGTACCTATCAGTTGGATCTTTGGTGGACAAGTGAGGGTCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00061 ko01040 ko01212 map00061 map01040 map01212

Pfam Domains

Protein Families

FA_desaturase_2

Protein Analysis

357

Amino Acids

41.17

Weight (kDa)

6.02

Isoelectric Point (pI)

42.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FA_desaturase_2	PF03405	48 - 252	1.7e-92	Fatty acid desaturase
FA_desaturase_2	PF03405	253 - 349	8.3e-31	Fatty acid desaturase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43710 AT2G43710
fragaria_vesca	FvH4_4g10110 FvH4_4g10110
malus_domestica	MD04G1048200.v1.1
prunus_persica	Prupe.1G203700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0405901
rosa_laevigata	RLG00000008819
rosa_multiflora	Rmu_sc0001200.1_g000011
rosa_roxburghii	Rroxscaffold_5G00350440
rosa_rugosa	Rorug04G0062600
rosa_samantha	Rh4AG134100 Rh4BG129200 Rh4CG142000 Rh4DG128500
rosa_wichuraiana	Rw4G010920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	831
AccBSI	CCGCTC	1 cut(s)	773
AccI	GTMKAC	1 cut(s)	519
AccII	CGCG	1 cut(s)	99
AciI	CCGC	4 cut(s)	636, 705, 740, 771
AclWI	GGATC	3 cut(s)	566, 579, 1049
AcoI	YGGCCR	1 cut(s)	221
AcuI	CTGAAG	2 cut(s)	381, 483
AcyI	GRCGYC	1 cut(s)	366
AfaI	GTAC	3 cut(s)	66, 709, 1029
AfiI	CCNNNNNNNGG	3 cut(s)	598, 628, 739
AflIII	ACRYGT	1 cut(s)	522
AgsI	TTSAA	7 cut(s)	157, 202, 413, 429, 688, 905, 998
AjnI	CCWGG	1 cut(s)	621
AluBI	AGCT	1 cut(s)	25
AluI	AGCT	1 cut(s)	25
Alw21I	GWGCWC	2 cut(s)	27, 183
AlwI	GGATC	3 cut(s)	566, 579, 1049
AlwNI	CAGNNNCTG	1 cut(s)	553
Ama87I	CYCGRG	1 cut(s)	575
AoxI	GGCC	5 cut(s)	221, 600, 741, 931, 963
ApeKI	GCWGC	1 cut(s)	832
Asp700I	GAANNNNTTC	2 cut(s)	83, 993
AspLEI	GCGC	5 cut(s)	99, 697, 732, 832, 837
AspS9I	GGNCC	4 cut(s)	481, 540, 931, 964
AsuC2I	CCSGG	2 cut(s)	576, 577
AsuHPI	GGTGA	5 cut(s)	272, 593, 791, 915, 1012
AvaI	CYCGRG	1 cut(s)	575
AvaII	GGWCC	2 cut(s)	481, 540
AxyI	CCTNAGG	1 cut(s)	927
BaeI	ACNNNNGTAYC	2 cut(s)	1011, 1044
BalI	TGGCCA	1 cut(s)	223
BamHI	GGATCC	1 cut(s)	571
BanII	GRGCYC	1 cut(s)	27
BbsI	GAAGAC	2 cut(s)	369, 471
Bbv12I	GWGCWC	2 cut(s)	27, 183
BbvCI	CCTCAGC	1 cut(s)	93
BbvI	GCAGC	1 cut(s)	844
BccI	CCATC	3 cut(s)	263, 398, 782
BciT130I	CCWGG	1 cut(s)	623
BclI	TGATCA	2 cut(s)	354, 796
BcnI	CCSGG	2 cut(s)	576, 577
BfaI	CTAG	1 cut(s)	164
BfoI	RGCGCY	2 cut(s)	733, 838
BisI	GCNGC	2 cut(s)	741, 833
BlpI	GCTNAGC	1 cut(s)	177
BlsI	GCNGC	2 cut(s)	742, 834
Bme1390I	CCNGG	3 cut(s)	576, 577, 623
Bme18I	GGWCC	2 cut(s)	481, 540
BmeT110I	CYCGRG	1 cut(s)	575
BmgT120I	GGNCC	4 cut(s)	481, 540, 931, 964
BmiI	GGNNCC	2 cut(s)	482, 573
BmrFI	CCNGG	3 cut(s)	576, 577, 623
BmrI	ACTGGG	1 cut(s)	181
BmsI	GCATC	1 cut(s)	378
BmuI	ACTGGG	1 cut(s)	181
BpiI	GAAGAC	2 cut(s)	369, 471
BpmI	CTGGAG	2 cut(s)	896, 1004
Bpu10I	CCTNAGC	3 cut(s)	26, 93, 942
Bpu1102I	GCTNAGC	1 cut(s)	177
BpuEI	CTTGAG	2 cut(s)	213, 299
BpuMI	CCSGG	2 cut(s)	576, 577
BsaBI	GATNNNNATC	1 cut(s)	758
BsaHI	GRCGYC	1 cut(s)	366
BsaJI	CCNNGG	4 cut(s)	575, 576, 669, 838
BsaWI	WCCGGW	1 cut(s)	914
Bsc4I	CCNNNNNNNGG	3 cut(s)	598, 628, 739
Bse1I	ACTGG	6 cut(s)	176, 262, 514, 822, 879, 987
Bse21I	CCTNAGG	1 cut(s)	927
Bse8I	GATNNNNATC	1 cut(s)	758
BseBI	CCWGG	1 cut(s)	623
BseDI	CCNNGG	4 cut(s)	575, 576, 669, 838
BseGI	GGATG	2 cut(s)	274, 409
BseJI	GATNNNNATC	1 cut(s)	758
BseLI	CCNNNNNNNGG	3 cut(s)	598, 628, 739
BseMII	CTCAG	5 cut(s)	40, 107, 168, 918, 956
BseNI	ACTGG	6 cut(s)	176, 262, 514, 822, 879, 987
BseXI	GCAGC	1 cut(s)	844
Bsh1236I	CGCG	1 cut(s)	99
BshFI	GGCC	5 cut(s)	223, 602, 743, 933, 965
BsiHKAI	GWGCWC	2 cut(s)	27, 183
BsiHKCI	CYCGRG	1 cut(s)	575
BsiSI	CCGG	2 cut(s)	576, 915
BslFI	GGGAC	4 cut(s)	494, 511, 695, 1019
BslI	CCNNNNNNNGG	3 cut(s)	598, 628, 739
BsmFI	GGGAC	4 cut(s)	494, 511, 695, 1019
BsnI	GGCC	5 cut(s)	223, 602, 743, 933, 965
BsoBI	CYCGRG	1 cut(s)	575
Bsp1286I	GDGCHC	2 cut(s)	27, 183
Bsp1407I	TGTACA	1 cut(s)	64
Bsp143I	GATC	4 cut(s)	354, 571, 796, 1041
Bsp1720I	GCTNAGC	1 cut(s)	177
BspACI	CCGC	4 cut(s)	636, 705, 740, 771
BspANI	GGCC	5 cut(s)	223, 602, 743, 933, 965
BspCNI	CTCAG	5 cut(s)	39, 106, 169, 919, 955
BspFNI	CGCG	1 cut(s)	99
BspLI	GGNNCC	2 cut(s)	482, 573
BspPI	GGATC	3 cut(s)	566, 579, 1049
BsrBI	CCGCTC	1 cut(s)	773
BsrGI	TGTACA	1 cut(s)	64
BsrI	ACTGG	6 cut(s)	176, 262, 514, 822, 879, 987
BssECI	CCNNGG	4 cut(s)	575, 576, 669, 838
BssMI	GATC	4 cut(s)	354, 571, 796, 1041
BssNI	GRCGYC	1 cut(s)	366
BssT1I	CCWWGG	2 cut(s)	669, 838
Bst2UI	CCWGG	1 cut(s)	623
BstACI	GRCGYC	1 cut(s)	366
BstAPI	GCANNNNNTGC	1 cut(s)	458
BstAUI	TGTACA	1 cut(s)	64
BstC8I	GCNNGC	3 cut(s)	6, 10, 771
BstDEI	CTNAG	7 cut(s)	26, 93, 177, 296, 927, 942, 968
BstF5I	GGATG	2 cut(s)	274, 409
BstFNI	CGCG	1 cut(s)	99
BstH2I	RGCGCY	2 cut(s)	733, 838
BstHHI	GCGC	5 cut(s)	99, 697, 732, 832, 837
BstKTI	GATC	4 cut(s)	357, 574, 799, 1044
BstMBI	GATC	4 cut(s)	354, 571, 796, 1041
BstMWI	GCNNNNNNNGC	3 cut(s)	103, 458, 665
BstNI	CCWGG	1 cut(s)	623
BstNSI	RCATGY	2 cut(s)	8, 526
BstSCI	CCNGG	3 cut(s)	574, 575, 621
BstUI	CGCG	1 cut(s)	99
BstV1I	GCAGC	1 cut(s)	844
BstV2I	GAAGAC	2 cut(s)	369, 471
BstX2I	RGATCY	2 cut(s)	571, 1041
BstYI	RGATCY	2 cut(s)	571, 1041
Bsu36I	CCTNAGG	1 cut(s)	927
BsuRI	GGCC	5 cut(s)	223, 602, 743, 933, 965
BtsCI	GGATG	2 cut(s)	274, 409
BtsI	GCAGTG	1 cut(s)	654
BtsIMutI	CAGTG	4 cut(s)	357, 507, 654, 829
Cac8I	GCNNGC	3 cut(s)	6, 10, 771
CaiI	CAGNNNCTG	1 cut(s)	553
CfoI	GCGC	5 cut(s)	99, 697, 732, 832, 837
Cfr13I	GGNCC	4 cut(s)	481, 540, 931, 964
Cfr9I	CCCGGG	1 cut(s)	575
CseI	GACGC	1 cut(s)	374
Csp6I	GTAC	3 cut(s)	65, 708, 1028
CviAII	CATG	9 cut(s)	5, 88, 352, 453, 476, 523, 647, 677, 734
CviQI	GTAC	3 cut(s)	65, 708, 1028
DdeI	CTNAG	7 cut(s)	26, 93, 177, 296, 927, 942, 968
DpnI	GATC	4 cut(s)	356, 573, 798, 1043
DpnII	GATC	4 cut(s)	354, 571, 796, 1041
EaeI	YGGCCR	1 cut(s)	221
Ecl136II	GAGCTC	1 cut(s)	25
Eco130I	CCWWGG	2 cut(s)	669, 838
Eco24I	GRGCYC	1 cut(s)	27
Eco47I	GGWCC	2 cut(s)	481, 540
Eco53kI	GAGCTC	1 cut(s)	25
Eco57I	CTGAAG	2 cut(s)	381, 483
Eco81I	CCTNAGG	1 cut(s)	927
Eco88I	CYCGRG	1 cut(s)	575
EcoICRI	GAGCTC	1 cut(s)	25
EcoO109I	RGGNCCY	1 cut(s)	481
EcoRII	CCWGG	1 cut(s)	621
EcoT14I	CCWWGG	2 cut(s)	669, 838
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	1 cut(s)	27
ErhI	CCWWGG	2 cut(s)	669, 838
FaeI	CATG	9 cut(s)	8, 91, 355, 456, 479, 526, 650, 680, 737
FalI	AAGNNNNNCTT	4 cut(s)	222, 254, 354, 386
FaqI	GGGAC	4 cut(s)	494, 511, 695, 1019
FatI	CATG	9 cut(s)	4, 87, 351, 452, 475, 522, 646, 676, 733
FauI	CCCGC	1 cut(s)	778
FbaI	TGATCA	2 cut(s)	354, 796
FblI	GTMKAC	1 cut(s)	519
Fnu4HI	GCNGC	2 cut(s)	741, 833
FokI	GGATG	2 cut(s)	281, 416
FriOI	GRGCYC	1 cut(s)	27
Fsp4HI	GCNGC	2 cut(s)	741, 833
FspBI	CTAG	1 cut(s)	164
FspI	TGCGCA	1 cut(s)	831
GlaI	GCGC	5 cut(s)	98, 696, 731, 831, 836
GluI	GCNGC	2 cut(s)	741, 833
GsuI	CTGGAG	2 cut(s)	896, 1004
HaeII	RGCGCY	2 cut(s)	733, 838
HaeIII	GGCC	5 cut(s)	223, 602, 743, 933, 965
HapII	CCGG	2 cut(s)	576, 915
HgaI	GACGC	1 cut(s)	374
HhaI	GCGC	5 cut(s)	99, 697, 732, 832, 837
Hin1I	GRCGYC	1 cut(s)	366
Hin1II	CATG	9 cut(s)	8, 91, 355, 456, 479, 526, 650, 680, 737
Hin6I	GCGC	5 cut(s)	97, 695, 730, 830, 835
HinP1I	GCGC	5 cut(s)	97, 695, 730, 830, 835
HincII	GTYRAC	2 cut(s)	211, 520
HindII	GTYRAC	2 cut(s)	211, 520
HinfI	GANTC	3 cut(s)	193, 316, 516
HpaII	CCGG	2 cut(s)	576, 915
HphI	GGTGA	5 cut(s)	272, 593, 791, 915, 1012
Hpy166II	GTNNAC	4 cut(s)	67, 211, 520, 1052
Hpy188I	TCNGA	5 cut(s)	347, 375, 555, 721, 1070
Hpy188III	TCNNGA	9 cut(s)	28, 113, 230, 249, 278, 320, 413, 794, 944
Hpy8I	GTNNAC	4 cut(s)	67, 211, 520, 1052
HpyAV	CCTTC	5 cut(s)	73, 380, 494, 613, 892
HpyCH4V	TGCA	2 cut(s)	4, 12
HpyF10VI	GCNNNNNNNGC	3 cut(s)	103, 458, 665
HpyF3I	CTNAG	7 cut(s)	26, 93, 177, 296, 927, 942, 968
Hsp92I	GRCGYC	1 cut(s)	366
Hsp92II	CATG	9 cut(s)	8, 91, 355, 456, 479, 526, 650, 680, 737
HspAI	GCGC	5 cut(s)	97, 695, 730, 830, 835
Ksp22I	TGATCA	2 cut(s)	354, 796
Kzo9I	GATC	4 cut(s)	354, 571, 796, 1041
LmnI	GCTCC	1 cut(s)	673
Lsp1109I	GCAGC	1 cut(s)	844
LweI	GCATC	1 cut(s)	378
MaeI	CTAG	1 cut(s)	164
MalI	GATC	4 cut(s)	356, 573, 798, 1043
MbiI	CCGCTC	1 cut(s)	773
MboI	GATC	4 cut(s)	354, 571, 796, 1041
MboII	GAAGA	3 cut(s)	230, 374, 476
MflI	RGATCY	2 cut(s)	571, 1041
MhlI	GDGCHC	2 cut(s)	27, 183
MlsI	TGGCCA	1 cut(s)	223
MluCI	AATT	5 cut(s)	58, 70, 117, 531, 998
MluNI	TGGCCA	1 cut(s)	223
MlyI	GAGTC	1 cut(s)	525
MmeI	TCCRAC	3 cut(s)	325, 398, 1019
Mox20I	TGGCCA	1 cut(s)	223
Mph1103I	ATGCAT	1 cut(s)	6
MroXI	GAANNNNTTC	2 cut(s)	83, 993
MscI	TGGCCA	1 cut(s)	223
MseI	TTAA	3 cut(s)	42, 393, 1001
Msp20I	TGGCCA	1 cut(s)	223
MspI	CCGG	2 cut(s)	576, 915
MspR9I	CCNGG	3 cut(s)	576, 577, 623
MteI	GCGCNGCGC	1 cut(s)	833
MvaI	CCWGG	1 cut(s)	623
MvnI	CGCG	1 cut(s)	99
MwoI	GCNNNNNNNGC	3 cut(s)	103, 458, 665
NciI	CCSGG	2 cut(s)	576, 577
NdeII	GATC	4 cut(s)	354, 571, 796, 1041
NlaIII	CATG	9 cut(s)	8, 91, 355, 456, 479, 526, 650, 680, 737
NlaIV	GGNNCC	2 cut(s)	482, 573
NsbI	TGCGCA	1 cut(s)	831
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	2 cut(s)	8, 526
PaeI	GCATGC	1 cut(s)	8
PciI	ACATGT	1 cut(s)	522
PdmI	GAANNNNTTC	2 cut(s)	83, 993
PfeI	GAWTC	2 cut(s)	193, 316
PfoI	TCCNGGA	1 cut(s)	621
PkrI	GCNGC	2 cut(s)	742, 834
PleI	GAGTC	1 cut(s)	524
PpsI	GAGTC	1 cut(s)	524
PpuMI	RGGWCCY	1 cut(s)	481
PscI	ACATGT	1 cut(s)	522
Psp124BI	GAGCTC	1 cut(s)	27
Psp5II	RGGWCCY	1 cut(s)	481
Psp6I	CCWGG	1 cut(s)	621
PspGI	CCWGG	1 cut(s)	621
PspN4I	GGNNCC	2 cut(s)	482, 573
PspPI	GGNCC	4 cut(s)	481, 540, 931, 964
PspPPI	RGGWCCY	1 cut(s)	481
PsrI	GAACNNNNNNTAC	2 cut(s)	578, 610
PstNI	CAGNNNCTG	1 cut(s)	553
PsuI	RGATCY	2 cut(s)	571, 1041
RsaI	GTAC	3 cut(s)	66, 709, 1029
RsaNI	GTAC	3 cut(s)	65, 708, 1028
SacI	GAGCTC	1 cut(s)	27
SalI	GTCGAC	1 cut(s)	518
SaqAI	TTAA	3 cut(s)	42, 393, 1001
SatI	GCNGC	2 cut(s)	741, 833
Sau3AI	GATC	4 cut(s)	354, 571, 796, 1041
Sau96I	GGNCC	4 cut(s)	481, 540, 931, 964
SchI	GAGTC	1 cut(s)	525
ScrFI	CCNGG	3 cut(s)	576, 577, 623
SduI	GDGCHC	2 cut(s)	27, 183
SfaNI	GCATC	1 cut(s)	378
SinI	GGWCC	2 cut(s)	481, 540
SmaI	CCCGGG	1 cut(s)	577
SmlI	CTYRAG	2 cut(s)	228, 278
SmoI	CTYRAG	2 cut(s)	228, 278
SphI	GCATGC	1 cut(s)	8
Sse9I	AATT	5 cut(s)	58, 70, 117, 531, 998
SsiI	CCGC	4 cut(s)	636, 705, 740, 771
SspMI	CTAG	1 cut(s)	164
SstI	GAGCTC	1 cut(s)	27
StyD4I	CCNGG	3 cut(s)	574, 575, 621
StyI	CCWWGG	2 cut(s)	669, 838
TaqI	TCGA	2 cut(s)	250, 519
TasI	AATT	5 cut(s)	58, 70, 117, 531, 998
TatI	WGTACW	1 cut(s)	64
TauI	GCSGC	1 cut(s)	743
TfiI	GAWTC	2 cut(s)	193, 316
Tru1I	TTAA	3 cut(s)	42, 393, 1001
Tru9I	TTAA	3 cut(s)	42, 393, 1001
TscAI	CASTG	4 cut(s)	364, 514, 661, 829
TseI	GCWGC	1 cut(s)	832
TspDTI	ATGAA	4 cut(s)	76, 432, 595, 606
TspGWI	ACGGA	3 cut(s)	281, 880, 1015
TspMI	CCCGGG	1 cut(s)	575
TspRI	CASTG	4 cut(s)	364, 514, 661, 829
VpaK11BI	GGWCC	2 cut(s)	481, 540
XceI	RCATGY	2 cut(s)	8, 526
XmaI	CCCGGG	1 cut(s)	575
XmiI	GTMKAC	1 cut(s)	519
XmnI	GAANNNNTTC	2 cut(s)	83, 993
XspI	CTAG	1 cut(s)	164
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.