AT2G44440

emsy N terminus domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009852
Physical Location & Seq
Reverse (-)
18336400 .. 18339275
2876 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44440.1

Sequence Viewer

Length: 1290 bp
ATGGACTGCAAATCGTCTGATAGCAGTGGAACTGATGATGATCTGCCACCGTCGCATAGAGTTCCACGAGGAGGAGGAGGAGGAAGAGGAGGACGCGTGGCTGGTAATGGAAGACCTTTGAATCTTCCGCCATCTTACCCAAAGATGTATGATGATCTTGCTGCGGATATGGAAGCTCAGATTCACCAGATTGAGAAGGAAGCATATATCTCTGTTTTAAGAGCCTTTAAAGCCCAAGGAGATGCTATTTCATGGGAAAAAGAAAGTGTCATAACTGAATTACGTAAAGAGTTGAGTTTATCGAATGAGGAGCATAGAGAGCTTCTAGGCCGTGTTAACTCAGATGATACAATACGAAGGATAAGGGAATGGAGACAATCTGGAGGAATGCAGCCAAGTATGCGCAATGCTGCTCAAGTGGTTCACGATACATTGCCAAGCCCTTCTGTTTCAGCTTCTATGAAGACGCACAAACCAAACCAGCCAATTCCTTCTCAACCATTCGCTAGTTCTTCACCTTCTTTTCATCCTCAAGCTGATCCCACTCACCCGTTTGCTTCATCGACAGCTAAACGAGGACCTGTTCCTATTGTCAAGGGCAAAAAACACAAACCAGTTTTTCCTGGCTCGTCTTCCACAAAACATGCCCCATACCATCCTTCAGATCAACCTCCACGAGGACAAGTCATGAACAGATTACCATCTGTTCCTGCCAGTTCAAGTGAACCCACAAACGGAATCGATCCTGAATCTTTTTTAGGAAGAAGAGTTAGAACAAAGTGGCCTGAGGACAATACATTTTACGACGCTATCATTACCCAGTATAATCCAGTTGAGGGTCGTCATGCTTTAGTTTATGACATTGCAACACCTTCTGAGACATGGGAATGGGTTAGACTCTCAGAGATATCTCCTGGGGATATAGAGTGGATAGGAGAGGATCCTGGGCTTGGTAATCGATATAATGGACAAGGTCATGGACTGAATAGAACTACCGGACCCAACTGTGTTCCACAACGAGGAAGCGGTTTGGAGAAGAACACTATCAGAAAGGGTTTCAGAACATCACAGAATGGAACTGGAAAAAAGAAACATCTCGATATACGAATCCGCCAAACAGATGTCCTAATCAGAGAGGTGGAGAGAGTTCTTCGTTCACACAATCCAGATCCTTACGAAGTTGAAAGGGCTAAGAGAGTTTTGGAGGAGCACGAGCATGCACTTGTGGGCGCAATTGCAAAGCTTGGAGATATTTCCGATGGAGAAAACGAAGGTGCATTTCGTCGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

429

Amino Acids

47.45

Weight (kDa)

8.67

Isoelectric Point (pI)

48.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 59 - 127 2.2e-30 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 404
AccII CGCG 1 cut(s) 96
AciI CCGC 4 cut(s) 128, 164, 1026, 1111
AclWI GGATC 5 cut(s) 533, 737, 935, 948, 1163
AcuI CTGAAG 1 cut(s) 645
AfiI CCNNNNNNNGG 6 cut(s) 71, 677, 734, 836, 950, 1019
AflIII ACRYGT 1 cut(s) 94
AgsI TTSAA 3 cut(s) 121, 720, 1184
AjnI CCWGG 3 cut(s) 622, 913, 943
AluBI AGCT 6 cut(s) 176, 322, 455, 536, 569, 1243
AluI AGCT 6 cut(s) 176, 322, 455, 536, 569, 1243
Alw21I GWGCWC 1 cut(s) 1212
Alw26I GTCTC 2 cut(s) 367, 872
AlwI GGATC 5 cut(s) 533, 737, 935, 948, 1163
AoxI GGCC 2 cut(s) 328, 782
ApeKI GCWGC 3 cut(s) 161, 391, 410
AspLEI GCGC 2 cut(s) 405, 1232
AspS9I GGNCC 2 cut(s) 578, 998
AsuHPI GGTGA 3 cut(s) 176, 507, 539
AvaII GGWCC 2 cut(s) 578, 998
AxyI CCTNAGG 1 cut(s) 786
BamHI GGATCC 1 cut(s) 940
BauI CACGAG 3 cut(s) 66, 675, 1211
BbsI GAAGAC 3 cut(s) 118, 470, 624
Bbv12I GWGCWC 1 cut(s) 1212
BbvI GCAGC 3 cut(s) 148, 397, 403
BccI CCATC 4 cut(s) 139, 663, 709, 1253
BceAI ACGGC 1 cut(s) 315
BciT130I CCWGG 3 cut(s) 624, 915, 945
BcoDI GTCTC 2 cut(s) 367, 872
BfaI CTAG 2 cut(s) 326, 507
BisI GCNGC 3 cut(s) 162, 392, 411
BlsI GCNGC 3 cut(s) 163, 393, 412
Bme1390I CCNGG 3 cut(s) 624, 915, 945
Bme18I GGWCC 2 cut(s) 578, 998
BmgT120I GGNCC 2 cut(s) 578, 998
BmiI GGNNCC 2 cut(s) 942, 1000
BmrFI CCNGG 3 cut(s) 624, 915, 945
BmrI ACTGGG 1 cut(s) 814
BmsI GCATC 1 cut(s) 232
BmuI ACTGGG 1 cut(s) 814
BpiI GAAGAC 3 cut(s) 118, 470, 624
BpmI CTGGAG 1 cut(s) 402
BpuEI CTTGAG 2 cut(s) 399, 516
Bsa29I ATCGAT 2 cut(s) 741, 958
BsaAI YACGTR 1 cut(s) 284
BsaBI GATNNNNATC 2 cut(s) 39, 700
BsaJI CCNNGG 3 cut(s) 235, 914, 944
BsaWI WCCGGW 1 cut(s) 995
BsaXI ACNNNNNCTCC 2 cut(s) 81, 111
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 677, 734, 836, 950, 1019
Bse1I ACTGG 5 cut(s) 614, 714, 820, 830, 1084
Bse21I CCTNAGG 1 cut(s) 786
Bse3DI GCAATG 3 cut(s) 412, 431, 861
Bse8I GATNNNNATC 2 cut(s) 39, 700
BseBI CCWGG 3 cut(s) 624, 915, 945
BseCI ATCGAT 2 cut(s) 741, 958
BseDI CCNNGG 3 cut(s) 235, 914, 944
BseGI GGATG 2 cut(s) 526, 655
BseJI GATNNNNATC 2 cut(s) 39, 700
BseLI CCNNNNNNNGG 6 cut(s) 71, 677, 734, 836, 950, 1019
BseMI GCAATG 3 cut(s) 412, 431, 861
BseMII CTCAG 5 cut(s) 191, 354, 777, 867, 915
BseNI ACTGG 5 cut(s) 614, 714, 820, 830, 1084
BseRI GAGGAG 7 cut(s) 84, 87, 90, 93, 102, 323, 1220
BseXI GCAGC 3 cut(s) 148, 397, 403
Bsh1236I CGCG 1 cut(s) 96
BshFI GGCC 2 cut(s) 330, 784
BshVI ATCGAT 2 cut(s) 741, 958
BsiHKAI GWGCWC 1 cut(s) 1212
BsiSI CCGG 1 cut(s) 996
BslI CCNNNNNNNGG 6 cut(s) 71, 677, 734, 836, 950, 1019
BsmAI GTCTC 2 cut(s) 367, 872
BsmI GAATGC 1 cut(s) 393
BsnI GGCC 2 cut(s) 330, 784
Bsp1286I GDGCHC 1 cut(s) 1212
Bsp143I GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
BspACI CCGC 4 cut(s) 128, 164, 1026, 1111
BspANI GGCC 2 cut(s) 330, 784
BspCNI CTCAG 5 cut(s) 190, 353, 778, 868, 914
BspDI ATCGAT 2 cut(s) 741, 958
BspFNI CGCG 1 cut(s) 96
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 2 cut(s) 942, 1000
BspPI GGATC 5 cut(s) 533, 737, 935, 948, 1163
BsrDI GCAATG 3 cut(s) 412, 431, 861
BsrI ACTGG 5 cut(s) 614, 714, 820, 830, 1084
BssECI CCNNGG 3 cut(s) 235, 914, 944
BssMI GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
BssSI CACGAG 3 cut(s) 66, 675, 1211
BssT1I CCWWGG 1 cut(s) 235
Bst2BI CACGAG 3 cut(s) 66, 675, 1211
Bst2UI CCWGG 3 cut(s) 624, 915, 945
Bst4CI ACNGT 2 cut(s) 51, 1007
Bst6I CTCTTC 2 cut(s) 79, 760
BstBAI YACGTR 1 cut(s) 284
BstC8I GCNNGC 1 cut(s) 1218
BstDEI CTNAG 6 cut(s) 177, 340, 786, 876, 901, 1191
BstENI CCTNNNNNAGG 1 cut(s) 675
BstF5I GGATG 2 cut(s) 526, 655
BstFNI CGCG 1 cut(s) 96
BstHHI GCGC 2 cut(s) 405, 1232
BstKTI GATC 7 cut(s) 43, 157, 541, 667, 745, 943, 1171
BstMAI GTCTC 2 cut(s) 367, 872
BstMBI GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
BstMWI GCNNNNNNNGC 4 cut(s) 52, 230, 319, 400
BstNI CCWGG 3 cut(s) 624, 915, 945
BstNSI RCATGY 2 cut(s) 647, 1220
BstSCI CCNGG 3 cut(s) 622, 913, 943
BstSNI TACGTA 1 cut(s) 284
BstUI CGCG 1 cut(s) 96
BstV1I GCAGC 3 cut(s) 148, 397, 403
BstV2I GAAGAC 3 cut(s) 118, 470, 624
BstX2I RGATCY 2 cut(s) 940, 1168
BstYI RGATCY 2 cut(s) 940, 1168
Bsu15I ATCGAT 2 cut(s) 741, 958
Bsu36I CCTNAGG 1 cut(s) 786
BsuRI GGCC 2 cut(s) 330, 784
BsuTUI ATCGAT 2 cut(s) 741, 958
BtsCI GGATG 2 cut(s) 526, 655
BtsI GCAGTG 1 cut(s) 31
BtsIMutI CAGTG 1 cut(s) 31
Cac8I GCNNGC 1 cut(s) 1218
CciI TCATGA 1 cut(s) 687
CfoI GCGC 2 cut(s) 405, 1232
Cfr13I GGNCC 2 cut(s) 578, 998
ClaI ATCGAT 2 cut(s) 741, 958
CseI GACGC 3 cut(s) 102, 475, 815
CviAII CATG 7 cut(s) 252, 644, 688, 845, 882, 977, 1217
DdeI CTNAG 6 cut(s) 177, 340, 786, 876, 901, 1191
DpnI GATC 7 cut(s) 42, 156, 540, 666, 744, 942, 1170
DpnII GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
DraI TTTAAA 1 cut(s) 229
Eam1104I CTCTTC 2 cut(s) 79, 760
EarI CTCTTC 2 cut(s) 79, 760
EciI GGCGGA 2 cut(s) 117, 1100
Eco105I TACGTA 1 cut(s) 284
Eco130I CCWWGG 1 cut(s) 235
Eco32I GATATC 1 cut(s) 909
Eco47I GGWCC 2 cut(s) 578, 998
Eco57I CTGAAG 1 cut(s) 645
Eco81I CCTNAGG 1 cut(s) 786
EcoNI CCTNNNNNAGG 1 cut(s) 675
EcoO109I RGGNCCY 1 cut(s) 578
EcoRII CCWGG 3 cut(s) 622, 913, 943
EcoRV GATATC 1 cut(s) 909
EcoT14I CCWWGG 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 7 cut(s) 255, 647, 691, 848, 885, 980, 1220
FatI CATG 7 cut(s) 251, 643, 687, 844, 881, 976, 1216
Fnu4HI GCNGC 3 cut(s) 162, 392, 411
FokI GGATG 2 cut(s) 513, 642
Fsp4HI GCNGC 3 cut(s) 162, 392, 411
FspBI CTAG 2 cut(s) 326, 507
FspI TGCGCA 1 cut(s) 404
GlaI GCGC 2 cut(s) 404, 1231
GluI GCNGC 3 cut(s) 162, 392, 411
GsuI CTGGAG 1 cut(s) 402
HaeIII GGCC 2 cut(s) 330, 784
HapII CCGG 1 cut(s) 996
HgaI GACGC 3 cut(s) 102, 475, 815
HhaI GCGC 2 cut(s) 405, 1232
Hin1II CATG 7 cut(s) 255, 647, 691, 848, 885, 980, 1220
Hin6I GCGC 2 cut(s) 403, 1230
HinP1I GCGC 2 cut(s) 403, 1230
HincII GTYRAC 1 cut(s) 337
HindII GTYRAC 1 cut(s) 337
HindIII AAGCTT 1 cut(s) 1241
HinfI GANTC 6 cut(s) 121, 181, 738, 749, 897, 1107
HpaI GTTAAC 1 cut(s) 337
HpaII CCGG 1 cut(s) 996
HphI GGTGA 3 cut(s) 176, 507, 539
Hpy166II GTNNAC 4 cut(s) 337, 424, 725, 1157
Hpy188III TCNNGA 6 cut(s) 381, 425, 688, 746, 1097, 1166
Hpy8I GTNNAC 4 cut(s) 337, 424, 725, 1157
Hpy99I CGWCG 3 cut(s) 55, 809, 1287
HpyAV CCTTC 8 cut(s) 190, 351, 453, 501, 528, 669, 882, 1265
HpyCH4III ACNGT 2 cut(s) 51, 1007
HpyCH4IV ACGT 1 cut(s) 283
HpyCH4V TGCA 6 cut(s) 9, 391, 866, 1220, 1238, 1277
HpyF10VI GCNNNNNNNGC 4 cut(s) 52, 230, 319, 400
HpyF3I CTNAG 6 cut(s) 177, 340, 786, 876, 901, 1191
HpySE526I ACGT 1 cut(s) 283
Hsp92II CATG 7 cut(s) 255, 647, 691, 848, 885, 980, 1220
HspAI GCGC 2 cut(s) 403, 1230
KspAI GTTAAC 1 cut(s) 337
Kzo9I GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
LmnI GCTCC 2 cut(s) 310, 1207
Lsp1109I GCAGC 3 cut(s) 148, 397, 403
LweI GCATC 1 cut(s) 232
MaeI CTAG 2 cut(s) 326, 507
MaeII ACGT 1 cut(s) 283
MalI GATC 7 cut(s) 42, 156, 540, 666, 744, 942, 1170
MboI GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
MfeI CAATTG 1 cut(s) 1233
MflI RGATCY 2 cut(s) 940, 1168
MhlI GDGCHC 1 cut(s) 1212
MluCI AATT 3 cut(s) 278, 486, 1233
MluI ACGCGT 1 cut(s) 94
MlyI GAGTC 1 cut(s) 891
MseI TTAA 3 cut(s) 218, 228, 336
MslI CAYNNNNRTG 2 cut(s) 886, 1215
MspI CCGG 1 cut(s) 996
MspR9I CCNGG 3 cut(s) 624, 915, 945
MunI CAATTG 1 cut(s) 1233
Mva1269I GAATGC 1 cut(s) 393
MvaI CCWGG 3 cut(s) 624, 915, 945
MvnI CGCG 1 cut(s) 96
MwoI GCNNNNNNNGC 4 cut(s) 52, 230, 319, 400
NdeII GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
NlaIII CATG 7 cut(s) 255, 647, 691, 848, 885, 980, 1220
NlaIV GGNNCC 2 cut(s) 942, 1000
NsbI TGCGCA 1 cut(s) 404
NspI RCATGY 2 cut(s) 647, 1220
PaeI GCATGC 1 cut(s) 1220
PagI TCATGA 1 cut(s) 687
PctI GAATGC 1 cut(s) 393
PfeI GAWTC 5 cut(s) 121, 181, 738, 749, 1107
PflFI GACNNNGTC 1 cut(s) 972
PkrI GCNGC 3 cut(s) 163, 393, 412
PleI GAGTC 1 cut(s) 891
PpsI GAGTC 1 cut(s) 891
Ppu21I YACGTR 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 578
Psp5II RGGWCCY 1 cut(s) 578
Psp6I CCWGG 3 cut(s) 622, 913, 943
PspGI CCWGG 3 cut(s) 622, 913, 943
PspN4I GGNNCC 2 cut(s) 942, 1000
PspPI GGNCC 2 cut(s) 578, 998
PspPPI RGGWCCY 1 cut(s) 578
PsuI RGATCY 2 cut(s) 940, 1168
PsyI GACNNNGTC 1 cut(s) 972
RseI CAYNNNNRTG 2 cut(s) 886, 1215
SaqAI TTAA 3 cut(s) 218, 228, 336
SatI GCNGC 3 cut(s) 162, 392, 411
Sau3AI GATC 7 cut(s) 40, 154, 538, 664, 742, 940, 1168
Sau96I GGNCC 2 cut(s) 578, 998
SchI GAGTC 1 cut(s) 891
ScrFI CCNGG 3 cut(s) 624, 915, 945
SduI GDGCHC 1 cut(s) 1212
SfaNI GCATC 1 cut(s) 232
SinI GGWCC 2 cut(s) 578, 998
SmiMI CAYNNNNRTG 2 cut(s) 886, 1215
SmlI CTYRAG 2 cut(s) 414, 531
SmoI CTYRAG 2 cut(s) 414, 531
SnaBI TACGTA 1 cut(s) 284
SphI GCATGC 1 cut(s) 1220
Sse9I AATT 3 cut(s) 278, 486, 1233
SsiI CCGC 4 cut(s) 128, 164, 1026, 1111
SspMI CTAG 2 cut(s) 326, 507
StyD4I CCNGG 3 cut(s) 622, 913, 943
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 2 cut(s) 51, 1007
TaiI ACGT 1 cut(s) 286
TaqI TCGA 5 cut(s) 302, 563, 741, 958, 1098
TasI AATT 3 cut(s) 278, 486, 1233
TfiI GAWTC 5 cut(s) 121, 181, 738, 749, 1107
Tru1I TTAA 3 cut(s) 218, 228, 336
Tru9I TTAA 3 cut(s) 218, 228, 336
TscAI CASTG 1 cut(s) 31
TseI GCWGC 3 cut(s) 161, 391, 410
TspDTI ATGAA 5 cut(s) 240, 476, 515, 549, 704
TspGWI ACGGA 1 cut(s) 750
TspRI CASTG 1 cut(s) 31
Tth111I GACNNNGTC 1 cut(s) 972
VpaK11BI GGWCC 2 cut(s) 578, 998
XagI CCTNNNNNAGG 1 cut(s) 675
XceI RCATGY 2 cut(s) 647, 1220
XspI CTAG 2 cut(s) 326, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.