FvH4_7g00190

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0009852
Physical Location & Seq
Reverse (-)
781160 .. 786575
5416 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g00190.t1

Sequence Viewer

Length: 1311 bp
ATGGAGCACGAGCCATACGATAGCAGCGGAACTGATGACGACCTTCCTCCATCACACCAAAATAGAATTCCTCGAGGGGGTCGTGTTGCAGGGAATGGAAGGTCTGCTGTGGCTTCTGTCCCGTATTCCAGGATGTATGGTGACACTGGCATGGAAGCACAAATTCATCAGCTTGAGAAAGAAGCATACAGTTCAGTTCTAAGAGCCTTTAAAGCTCAAGCTGATCAAATTACTTGGGAAAAGGAAAGTGTGATAACAGAACTTAGAAAGGAGTTGAGGTTATCGAATGAGGAGCACAGAGAACTTCTGGGACGGGTTAATGCTGATGATGTCCTACAGAGGATAAGGGAGTGGAGACAGGGAGGTGGAGTTCAACTGGGCATGCTCAATACTGTTCAAGCTGTTCATGATCCATTGCCGAGTCCTACTGTTTCTGCATCTCGGAAGAAACAGAAGACTACACAATCAATACATTCTCAATCCTTTGTAGCCCCATCACCACCATTTCTTCCACAGCCATCAACAACATCCCACCAGCCATCTTCATCTAATGTAAAGAGAGGATCTGTCCCAGTCCCAGCCCCAGCAGCCAAGGGCAAGAAACATAAATCTGGTCAAATTTTACCTGGTGCTTCTTCAATAAAGCCGCACCCTTCTTCAGGAAGGGGTCCAGTTTCTAATAGAGTCTCTTCTGGTGCAATTAGGAATGAGCCTGTGGAAGGAGCGACACTTAATCCTTTCATTGGGAAAAAAGTTAGGACTAGGTGGCCCGAGGACAACAGCTTTTATGAAGCTGTTATAACGGACTATAATGCAGCTAAGGGATTACATGTACTAGTCTACGATATGAATTCTGCTGATGAATCGTGGGAATGGGTTAATCTATCAGAGATATCTCCTGAGGATATTCAGTGGGTAGACGAAGATCCTGGTATCTCTCATCGTGGGGGTTATGGTGGATCAGGGCATGGGATGAATAGATCTGTAGGCCGTGACAGTGTTCCAGGTTCTGGAAGAGGTAGAGGAATCCGAAAGGGTCAATCAAGAAAAGATTTTTTGCCATCCCAGAATGGCATTGGAAAGAAGGCGCCAGATGATATACATTTACTTCACACAGATACTTTAATTAAGGAGGTAGAGAGAGTCTTTGGTGCAAATCATCCAGACCCTGTTGAAATTGATAAAGCAAAGAAAGTTTTGAAAGATCATGAACAAGCACTTATTGATGCAATTGCAAAGCTCACAGATATTTCTGATGGTGAAAGTGCTGAGGTTGGCCACCAATTCTTACGTGGGCAACCAATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

437

Amino Acids

47.63

Weight (kDa)

7.02

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 53 - 121 1.2e-29 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 800
AbsI CCTCGAGG 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 1087
AccB7I CCANNNNNTGG 1 cut(s) 1010
AccI GTMKAC 2 cut(s) 840, 918
AciI CCGC 2 cut(s) 27, 647
AclWI GGATC 4 cut(s) 404, 571, 920, 967
AcoI YGGCCR 1 cut(s) 1276
AcsI RAATTY 4 cut(s) 66, 162, 618, 850
AcuI CTGAAG 1 cut(s) 642
AcyI GRCGYC 1 cut(s) 1088
AfaI GTAC 1 cut(s) 834
AfiI CCNNNNNNNGG 5 cut(s) 77, 659, 719, 743, 1010
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 5 cut(s) 374, 398, 639, 1175, 1201
AhlI ACTAGT 1 cut(s) 835
AjnI CCWGG 4 cut(s) 128, 625, 928, 1003
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AloI GAACNNNNNNTCC 2 cut(s) 354, 386
AluBI AGCT 8 cut(s) 172, 215, 221, 401, 783, 794, 818, 1240
AluI AGCT 8 cut(s) 172, 215, 221, 401, 783, 794, 818, 1240
Alw21I GWGCWC 2 cut(s) 9, 297
Alw26I GTCTC 2 cut(s) 349, 691
AlwI GGATC 4 cut(s) 404, 571, 920, 967
AlwNI CAGNNNCTG 2 cut(s) 1010, 1169
Ama87I CYCGRG 2 cut(s) 72, 770
AoxI GGCC 3 cut(s) 767, 988, 1276
ApeKI GCWGC 3 cut(s) 24, 587, 815
ApoI RAATTY 4 cut(s) 66, 162, 618, 850
AspLEI GCGC 1 cut(s) 1090
AspS9I GGNCC 2 cut(s) 668, 768
AsuHPI GGTGA 3 cut(s) 152, 489, 1271
AvaI CYCGRG 2 cut(s) 72, 770
AvaII GGWCC 1 cut(s) 668
AxyI CCTNAGG 1 cut(s) 900
BalI TGGCCA 1 cut(s) 1278
BanI GGYRCC 1 cut(s) 1087
BauI CACGAG 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 2 cut(s) 9, 297
BbvCI CCTCAGC 1 cut(s) 1269
BbvI GCAGC 3 cut(s) 36, 599, 827
BccI CCATC 6 cut(s) 58, 502, 526, 547, 1069, 1250
BceAI ACGGC 1 cut(s) 975
BciT130I CCWGG 4 cut(s) 130, 627, 930, 1005
BclI TGATCA 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 349, 691
BcuI ACTAGT 1 cut(s) 835
BfaI CTAG 2 cut(s) 762, 836
BfmI CTRYAG 2 cut(s) 335, 984
BfoI RGCGCY 1 cut(s) 1091
BglII AGATCT 1 cut(s) 980
BisI GCNGC 4 cut(s) 25, 588, 647, 816
BlsI GCNGC 4 cut(s) 26, 589, 648, 817
Bme1390I CCNGG 4 cut(s) 130, 627, 930, 1005
Bme18I GGWCC 1 cut(s) 668
BmeT110I CYCGRG 2 cut(s) 72, 770
BmgT120I GGNCC 2 cut(s) 668, 768
BmiI GGNNCC 2 cut(s) 669, 1089
BmrFI CCNGG 4 cut(s) 130, 627, 930, 1005
BmrI ACTGGG 2 cut(s) 386, 566
BmsI GCATC 2 cut(s) 446, 1216
BmuI ACTGGG 2 cut(s) 386, 566
BpiI GAAGAC 1 cut(s) 461
Bpu10I CCTNAGC 2 cut(s) 819, 1269
BpuEI CTTGAG 2 cut(s) 194, 201
BsaAI YACGTR 1 cut(s) 1292
BsaHI GRCGYC 1 cut(s) 1088
BsaJI CCNNGG 2 cut(s) 591, 771
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 354, 384
Bsc4I CCNNNNNNNGG 5 cut(s) 77, 659, 719, 743, 1010
Bse1I ACTGG 4 cut(s) 151, 381, 572, 671
Bse21I CCTNAGG 1 cut(s) 900
Bse3DI GCAATG 1 cut(s) 413
BseBI CCWGG 4 cut(s) 130, 627, 930, 1005
BseDI CCNNGG 2 cut(s) 591, 771
BseGI GGATG 5 cut(s) 138, 527, 978, 1061, 1159
BseLI CCNNNNNNNGG 5 cut(s) 77, 659, 719, 743, 1010
BseMI GCAATG 1 cut(s) 413
BseMII CTCAG 2 cut(s) 891, 1260
BseNI ACTGG 4 cut(s) 151, 381, 572, 671
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 3 cut(s) 36, 599, 827
BseYI CCCAGC 2 cut(s) 577, 583
BshFI GGCC 3 cut(s) 769, 990, 1278
BshNI GGYRCC 1 cut(s) 1087
BsiHKAI GWGCWC 2 cut(s) 9, 297
BsiHKCI CYCGRG 2 cut(s) 72, 770
BslFI GGGAC 4 cut(s) 104, 324, 554, 560
BslI CCNNNNNNNGG 5 cut(s) 77, 659, 719, 743, 1010
BsmAI GTCTC 2 cut(s) 349, 691
BsmFI GGGAC 4 cut(s) 104, 324, 554, 560
BsnI GGCC 3 cut(s) 769, 990, 1278
BsoBI CYCGRG 2 cut(s) 72, 770
Bsp1286I GDGCHC 2 cut(s) 9, 297
Bsp143I GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
BspACI CCGC 2 cut(s) 27, 647
BspANI GGCC 3 cut(s) 769, 990, 1278
BspCNI CTCAG 2 cut(s) 892, 1261
BspHI TCATGA 2 cut(s) 406, 1207
BspLI GGNNCC 2 cut(s) 669, 1089
BspPI GGATC 4 cut(s) 404, 571, 920, 967
BspT107I GGYRCC 1 cut(s) 1087
BsrDI GCAATG 1 cut(s) 413
BsrI ACTGG 4 cut(s) 151, 381, 572, 671
BssECI CCNNGG 2 cut(s) 591, 771
BssMI GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
BssNI GRCGYC 1 cut(s) 1088
BssSI CACGAG 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 591
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 4 cut(s) 130, 627, 930, 1005
Bst4CI ACNGT 4 cut(s) 191, 394, 430, 998
Bst6I CTCTTC 2 cut(s) 694, 1009
BstACI GRCGYC 1 cut(s) 1088
BstBAI YACGTR 1 cut(s) 1292
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 5 cut(s) 200, 263, 819, 900, 1269
BstENI CCTNNNNNAGG 2 cut(s) 657, 717
BstF5I GGATG 5 cut(s) 138, 527, 978, 1061, 1159
BstH2I RGCGCY 1 cut(s) 1091
BstHHI GCGC 1 cut(s) 1090
BstKTI GATC 7 cut(s) 226, 412, 566, 928, 962, 983, 1207
BstMAI GTCTC 2 cut(s) 349, 691
BstMBI GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
BstMWI GCNNNNNNNGC 2 cut(s) 212, 587
BstNI CCWGG 4 cut(s) 130, 627, 930, 1005
BstNSI RCATGY 2 cut(s) 385, 833
BstSCI CCNGG 4 cut(s) 128, 625, 928, 1003
BstSFI CTRYAG 2 cut(s) 335, 984
BstV1I GCAGC 3 cut(s) 36, 599, 827
BstV2I GAAGAC 1 cut(s) 461
BstX2I RGATCY 3 cut(s) 563, 925, 980
BstYI RGATCY 3 cut(s) 563, 925, 980
Bsu36I CCTNAGG 1 cut(s) 900
BsuRI GGCC 3 cut(s) 769, 990, 1278
BtsCI GGATG 5 cut(s) 138, 527, 978, 1061, 1159
BtsIMutI CAGTG 3 cut(s) 144, 917, 1003
Cac8I GCNNGC 1 cut(s) 383
CaiI CAGNNNCTG 2 cut(s) 1010, 1169
CciI TCATGA 2 cut(s) 406, 1207
CfoI GCGC 1 cut(s) 1090
Cfr13I GGNCC 2 cut(s) 668, 768
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 1 cut(s) 833
CviAII CATG 6 cut(s) 151, 382, 407, 830, 968, 1208
CviQI GTAC 1 cut(s) 833
DdeI CTNAG 5 cut(s) 200, 263, 819, 900, 1269
DinI GGCGCC 1 cut(s) 1089
DpnI GATC 7 cut(s) 225, 411, 565, 927, 961, 982, 1206
DpnII GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
DraI TTTAAA 1 cut(s) 211
EaeI YGGCCR 1 cut(s) 1276
Eam1104I CTCTTC 2 cut(s) 694, 1009
EarI CTCTTC 2 cut(s) 694, 1009
Eco130I CCWWGG 1 cut(s) 591
Eco32I GATATC 1 cut(s) 894
Eco47I GGWCC 1 cut(s) 668
Eco57I CTGAAG 1 cut(s) 642
Eco81I CCTNAGG 1 cut(s) 900
Eco88I CYCGRG 2 cut(s) 72, 770
EcoNI CCTNNNNNAGG 2 cut(s) 657, 717
EcoRI GAATTC 2 cut(s) 66, 850
EcoRII CCWGG 4 cut(s) 128, 625, 928, 1003
EcoRV GATATC 1 cut(s) 894
EcoT14I CCWWGG 1 cut(s) 591
EgeI GGCGCC 1 cut(s) 1089
EheI GGCGCC 1 cut(s) 1089
ErhI CCWWGG 1 cut(s) 591
FaeI CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
FaqI GGGAC 4 cut(s) 104, 324, 554, 560
FatI CATG 6 cut(s) 150, 381, 406, 829, 967, 1207
FbaI TGATCA 1 cut(s) 223
FblI GTMKAC 2 cut(s) 840, 918
Fnu4HI GCNGC 4 cut(s) 25, 588, 647, 816
FokI GGATG 5 cut(s) 145, 514, 985, 1048, 1146
Fsp4HI GCNGC 4 cut(s) 25, 588, 647, 816
FspBI CTAG 2 cut(s) 762, 836
GlaI GCGC 1 cut(s) 1089
GluI GCNGC 4 cut(s) 25, 588, 647, 816
GsaI CCCAGC 2 cut(s) 581, 587
HaeII RGCGCY 1 cut(s) 1091
HaeIII GGCC 3 cut(s) 769, 990, 1278
HhaI GCGC 1 cut(s) 1090
Hin1I GRCGYC 1 cut(s) 1088
Hin1II CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
Hin6I GCGC 1 cut(s) 1088
HinP1I GCGC 1 cut(s) 1088
HinfI GANTC 5 cut(s) 421, 684, 863, 1026, 1143
HphI GGTGA 3 cut(s) 152, 489, 1271
Hpy166II GTNNAC 2 cut(s) 841, 919
Hpy188I TCNGA 4 cut(s) 444, 889, 1031, 1255
Hpy188III TCNNGA 7 cut(s) 407, 660, 899, 1011, 1044, 1163, 1208
Hpy8I GTNNAC 2 cut(s) 841, 919
HpyAV CCTTC 6 cut(s) 53, 93, 657, 663, 713, 1078
HpyCH4III ACNGT 4 cut(s) 191, 394, 430, 998
HpyCH4IV ACGT 1 cut(s) 1291
HpyCH4V TGCA 7 cut(s) 89, 437, 698, 815, 1154, 1229, 1235
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 587
HpyF3I CTNAG 5 cut(s) 200, 263, 819, 900, 1269
HpySE526I ACGT 1 cut(s) 1291
Hsp92I GRCGYC 1 cut(s) 1088
Hsp92II CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
HspAI GCGC 1 cut(s) 1088
KasI GGCGCC 1 cut(s) 1087
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
LmnI GCTCC 3 cut(s) 4, 292, 722
Lsp1109I GCAGC 3 cut(s) 36, 599, 827
LweI GCATC 2 cut(s) 446, 1216
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 2 cut(s) 762, 836
MaeII ACGT 1 cut(s) 1291
MaeIII GTNAC 2 cut(s) 140, 992
MalI GATC 7 cut(s) 225, 411, 565, 927, 961, 982, 1206
MboI GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
MboII GAAGA 9 cut(s) 457, 466, 500, 534, 627, 648, 681, 935, 1026
MfeI CAATTG 1 cut(s) 1230
MflI RGATCY 3 cut(s) 563, 925, 980
MhlI GDGCHC 2 cut(s) 9, 297
MlsI TGGCCA 1 cut(s) 1278
MluNI TGGCCA 1 cut(s) 1278
Mly113I GGCGCC 1 cut(s) 1088
MlyI GAGTC 3 cut(s) 430, 693, 1152
Mox20I TGGCCA 1 cut(s) 1278
MscI TGGCCA 1 cut(s) 1278
MseI TTAA 6 cut(s) 210, 318, 732, 879, 1124, 1128
MslI CAYNNNNRTG 1 cut(s) 149
Msp20I TGGCCA 1 cut(s) 1278
MspA1I CMGCKG 1 cut(s) 27
MspR9I CCNGG 4 cut(s) 130, 627, 930, 1005
MunI CAATTG 1 cut(s) 1230
MvaI CCWGG 4 cut(s) 130, 627, 930, 1005
MwoI GCNNNNNNNGC 2 cut(s) 212, 587
NarI GGCGCC 1 cut(s) 1088
NdeII GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
NlaIII CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
NlaIV GGNNCC 2 cut(s) 669, 1089
NmeAIII GCCGAG 1 cut(s) 444
NmuCI GTSAC 2 cut(s) 140, 992
NspI RCATGY 2 cut(s) 385, 833
PacI TTAATTAA 1 cut(s) 1128
PaeI GCATGC 1 cut(s) 385
PaeR7I CTCGAG 1 cut(s) 72
PagI TCATGA 2 cut(s) 406, 1207
PciI ACATGT 1 cut(s) 829
PcsI WCGNNNNNNNCGW 2 cut(s) 15, 79
PfeI GAWTC 2 cut(s) 863, 1026
PflMI CCANNNNNTGG 1 cut(s) 1010
PfoI TCCNGGA 1 cut(s) 128
PkrI GCNGC 4 cut(s) 26, 589, 648, 817
PleI GAGTC 3 cut(s) 429, 692, 1151
PluTI GGCGCC 1 cut(s) 1091
PpsI GAGTC 3 cut(s) 429, 692, 1151
Ppu21I YACGTR 1 cut(s) 1292
PscI ACATGT 1 cut(s) 829
PsiI TTATAA 1 cut(s) 800
Psp6I CCWGG 4 cut(s) 128, 625, 928, 1003
PspFI CCCAGC 2 cut(s) 577, 583
PspGI CCWGG 4 cut(s) 128, 625, 928, 1003
PspN4I GGNNCC 2 cut(s) 669, 1089
PspPI GGNCC 2 cut(s) 668, 768
PspXI VCTCGAGB 1 cut(s) 72
PstNI CAGNNNCTG 2 cut(s) 1010, 1169
PsuI RGATCY 3 cut(s) 563, 925, 980
RsaI GTAC 1 cut(s) 834
RsaNI GTAC 1 cut(s) 833
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 6 cut(s) 210, 318, 732, 879, 1124, 1128
SatI GCNGC 4 cut(s) 25, 588, 647, 816
Sau3AI GATC 7 cut(s) 223, 409, 563, 925, 959, 980, 1204
Sau96I GGNCC 2 cut(s) 668, 768
SchI GAGTC 3 cut(s) 430, 693, 1152
ScrFI CCNGG 4 cut(s) 130, 627, 930, 1005
SduI GDGCHC 2 cut(s) 9, 297
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 2 cut(s) 446, 1216
SfcI CTRYAG 2 cut(s) 335, 984
SfoI GGCGCC 1 cut(s) 1089
Sfr274I CTCGAG 1 cut(s) 72
SinI GGWCC 1 cut(s) 668
SlaI CTCGAG 1 cut(s) 72
SmiMI CAYNNNNRTG 1 cut(s) 149
SmlI CTYRAG 3 cut(s) 72, 173, 216
SmoI CTYRAG 3 cut(s) 72, 173, 216
SpeI ACTAGT 1 cut(s) 835
SphI GCATGC 1 cut(s) 385
SsiI CCGC 2 cut(s) 27, 647
SspDI GGCGCC 1 cut(s) 1087
SspMI CTAG 2 cut(s) 762, 836
StyD4I CCNGG 4 cut(s) 128, 625, 928, 1003
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 4 cut(s) 191, 394, 430, 998
TaiI ACGT 1 cut(s) 1294
TaqI TCGA 2 cut(s) 73, 284
TatI WGTACW 1 cut(s) 832
TauI GCSGC 1 cut(s) 649
TfiI GAWTC 2 cut(s) 863, 1026
Tru1I TTAA 6 cut(s) 210, 318, 732, 879, 1124, 1128
Tru9I TTAA 6 cut(s) 210, 318, 732, 879, 1124, 1128
TscAI CASTG 3 cut(s) 151, 917, 1003
TseFI GTSAC 2 cut(s) 140, 992
TseI GCWGC 3 cut(s) 24, 587, 815
Tsp45I GTSAC 2 cut(s) 140, 992
TspDTI ATGAA 9 cut(s) 155, 395, 534, 730, 804, 863, 876, 989, 1224
TspGWI ACGGA 1 cut(s) 818
TspRI CASTG 3 cut(s) 151, 917, 1003
Van91I CCANNNNNTGG 1 cut(s) 1010
VpaK11BI GGWCC 1 cut(s) 668
XagI CCTNNNNNAGG 2 cut(s) 657, 717
XapI RAATTY 4 cut(s) 66, 162, 618, 850
XceI RCATGY 2 cut(s) 385, 833
XcmI CCANNNNNNNNNTGG 2 cut(s) 1073, 1289
XhoI CTCGAG 1 cut(s) 72
XmiI GTMKAC 2 cut(s) 840, 918
XspI CTAG 2 cut(s) 762, 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.