MD02G1318200.v1.1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009852
Physical Location & Seq
Forward (+)
37266737 .. 37273587
6851 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1318200.v1.1.491

Sequence Viewer

Length: 1302 bp
ATGGACTACGAGCCCTACGATAGCAGCGGAACTGATGACGATTTTCCTCCATCACGCCAACATAGAGTTCCCAGAGGGGGTCGCGTTACAGGGAATGGAAGATCTGGTGGGGGTTCTGTCCCATATCCTAGGATGTATGGTGAAACTGACATGGAAGCACAAATTCACCAGCTTGAACGAGAAGCATACAGTTCAGTTCTAAGAGCCTTTAAAGCTCAAGCTGATGCCATTACTTGGGAAAAGGAAGGTCTGTTAACAAACCTTAGAAAGGAGTTGAGGTTATCAAATGAGGAGCACAAAGAACTTCTAGGACGGGTTAATGCAGCAGATGATGTCATAGAGAGGATAAGGGACTGGAGACAGGCTGGTGGGGTTCAATCGGGCATGTTGAGTACTGTTCAAGCAGTGCATGATCCAATACCTAGTCCTACTGTCTCTGTATCACGCAAGAAACAGAAGATGACCCAATCAGTACATACGCAGTCCTTTGCTGGGCCAACACCACCATTTCATCCACAGGCAGTTACCACATCCCACCAGCCATCTTCATCTACTGTAAAACGAGGATCTGTCCCAGCCCCAGCCTCAGGAGCTAAGGGCAAGAAACATAAACAGGGTCAAATTTTGCCCGGTGCTTCTTCAATGAAGCAGTACCCTTCCTCGGGTCCAACAGGAAGGGGTCAAGTTTCGCATAGAGTTTCAATTGGTGACGTTGTAAATGAACCTGCTGAAGGAAAGACAAATGATTCCTTGATTGGGAGGAAAGTAAGGACTAGGTGGCCCGACGACAACACCTTTTATGAAGCCATTATAAAAGACTACAATCAAGCAGAGGGACGGCATCATCTAGTCTACGATATTAATTCGGCAAATGAAACATGGGAGTGGGTTAATTTATCAGAGATATCTCCAGAGGATATTCAATGGGTAGGTGAAGATCCTGGAATCTCTCATCGTGGGGGTTATAGTGGATCTGGTCATGGGATGAATAGATCTGTAGGCCGTGACAATGTTCCAGTTCCTGGAAGAGGTAGAGGGACCCCAAAGGGTCAAACAAGAAAAGATTTTCCGCCATCACAAAATGGCATTGGAAAGAAGGCACCGGACAATATCCAGTTACTTCACACAGATACTTTAATTAAGGAGGTAGAAAGGGTCTTTGGTGAAAACCATCCAGACTCTGTTGAAATTGAGAAAGCAAAGAAAGTGTTGAAAGATCATGAACAAGCACTTATTGATGCAATTGCAAAGCTTGCTGATATTTCTGATGGTGAAAGCGGACCTTTGCCTTTAAACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

434

Amino Acids

47.38

Weight (kDa)

6.99

Isoelectric Point (pI)

40.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 53 - 122 7.5e-27 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 812
Acc36I ACCTGC 1 cut(s) 733
AccB1I GGYRCC 1 cut(s) 1099
AccB7I CCANNNNNTGG 2 cut(s) 234, 1022
AccI GTMKAC 1 cut(s) 852
AccII CGCG 1 cut(s) 84
AciI CCGC 3 cut(s) 27, 1070, 1278
AclWI GGATC 4 cut(s) 407, 574, 932, 979
AcsI RAATTY 2 cut(s) 162, 621
AcuI CTGAAG 1 cut(s) 750
AfaI GTAC 3 cut(s) 394, 474, 653
AgsI TTSAA 8 cut(s) 176, 377, 401, 642, 702, 923, 1187, 1213
AjnI CCWGG 2 cut(s) 940, 1021
AjuI GAANNNNNNNTTGG 4 cut(s) 51, 83, 1143, 1175
AluBI AGCT 6 cut(s) 172, 215, 221, 593, 1252, 1299
AluI AGCT 6 cut(s) 172, 215, 221, 593, 1252, 1299
Alw21I GWGCWC 1 cut(s) 297
Alw26I GTCTC 2 cut(s) 352, 439
AlwI GGATC 4 cut(s) 407, 574, 932, 979
AlwNI CAGNNNCTG 2 cut(s) 1022, 1181
Ama87I CYCGRG 1 cut(s) 661
AoxI GGCC 3 cut(s) 494, 779, 1000
ApeKI GCWGC 2 cut(s) 24, 323
ApoI RAATTY 2 cut(s) 162, 621
AseI ATTAAT 1 cut(s) 861
AspA2I CCTAGG 1 cut(s) 128
AspS9I GGNCC 5 cut(s) 494, 665, 780, 1038, 1280
AsuC2I CCSGG 1 cut(s) 630
AsuHPI GGTGA 6 cut(s) 152, 158, 719, 944, 1175, 1283
AvaI CYCGRG 1 cut(s) 661
AvaII GGWCC 3 cut(s) 665, 1038, 1280
AvrII CCTAGG 1 cut(s) 128
AxyI CCTNAGG 1 cut(s) 586
BanI GGYRCC 1 cut(s) 1099
BanII GRGCYC 1 cut(s) 15
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 2 cut(s) 36, 335
BccI CCATC 5 cut(s) 58, 550, 1081, 1179, 1262
BceAI ACGGC 2 cut(s) 854, 987
BciT130I CCWGG 2 cut(s) 942, 1023
BcnI CCSGG 1 cut(s) 630
BcoDI GTCTC 2 cut(s) 352, 439
BfaI CTAG 5 cut(s) 129, 308, 423, 774, 848
BfmI CTRYAG 1 cut(s) 996
BfuAI ACCTGC 1 cut(s) 733
BglII AGATCT 2 cut(s) 101, 992
BisI GCNGC 2 cut(s) 25, 324
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 2 cut(s) 26, 325
BmcAI AGTACT 1 cut(s) 394
Bme1390I CCNGG 3 cut(s) 630, 942, 1023
Bme18I GGWCC 3 cut(s) 665, 1038, 1280
BmeT110I CYCGRG 1 cut(s) 661
BmgT120I GGNCC 5 cut(s) 494, 665, 780, 1038, 1280
BmiI GGNNCC 4 cut(s) 666, 1039, 1040, 1101
BmrFI CCNGG 3 cut(s) 630, 942, 1023
BmsI GCATC 3 cut(s) 214, 850, 1228
BpmI CTGGAG 2 cut(s) 376, 894
Bpu10I CCTNAGC 1 cut(s) 594
BpuEI CTTGAG 1 cut(s) 201
BpuMI CCSGG 1 cut(s) 630
BsaJI CCNNGG 2 cut(s) 128, 660
BsaWI WCCGGW 1 cut(s) 1102
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bse1I ACTGG 3 cut(s) 359, 1016, 1114
Bse21I CCTNAGG 1 cut(s) 586
BseBI CCWGG 2 cut(s) 942, 1023
BseDI CCNNGG 2 cut(s) 128, 660
BseGI GGATG 5 cut(s) 138, 511, 530, 990, 1171
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 3 cut(s) 359, 1016, 1114
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 36, 335
BseYI CCCAGC 3 cut(s) 491, 574, 580
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 3 cut(s) 496, 781, 1002
BshNI GGYRCC 1 cut(s) 1099
BsiHKAI GWGCWC 1 cut(s) 297
BsiHKCI CYCGRG 1 cut(s) 661
BsiSI CCGG 2 cut(s) 630, 1103
BslFI GGGAC 5 cut(s) 104, 365, 557, 849, 1051
BsmAI GTCTC 2 cut(s) 352, 439
BsmFI GGGAC 5 cut(s) 104, 365, 557, 849, 1051
BsnI GGCC 3 cut(s) 496, 781, 1002
BsoBI CYCGRG 1 cut(s) 661
Bsp1286I GDGCHC 2 cut(s) 15, 297
Bsp143I GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
BspACI CCGC 3 cut(s) 27, 1070, 1278
BspANI GGCC 3 cut(s) 496, 781, 1002
BspCNI CTCAG 1 cut(s) 599
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 1219
BspLI GGNNCC 4 cut(s) 666, 1039, 1040, 1101
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 4 cut(s) 407, 574, 932, 979
BspT107I GGYRCC 1 cut(s) 1099
BsrI ACTGG 3 cut(s) 359, 1016, 1114
BssECI CCNNGG 2 cut(s) 128, 660
BssMI GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
BssT1I CCWWGG 1 cut(s) 128
Bst2UI CCWGG 2 cut(s) 942, 1023
Bst4CI ACNGT 4 cut(s) 191, 397, 433, 556
Bst6I CTCTTC 1 cut(s) 1021
BstAPI GCANNNNNTGC 1 cut(s) 1253
BstC8I GCNNGC 1 cut(s) 1254
BstDEI CTNAG 4 cut(s) 200, 263, 586, 594
BstENI CCTNNNNNAGG 3 cut(s) 266, 729, 1026
BstF5I GGATG 5 cut(s) 138, 511, 530, 990, 1171
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 7 cut(s) 104, 415, 569, 940, 974, 995, 1219
BstMAI GTCTC 2 cut(s) 352, 439
BstMBI GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 212, 590, 1253
BstNI CCWGG 2 cut(s) 942, 1023
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 3 cut(s) 628, 940, 1021
BstSFI CTRYAG 1 cut(s) 996
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 36, 335
BstX2I RGATCY 5 cut(s) 101, 566, 937, 971, 992
BstYI RGATCY 5 cut(s) 101, 566, 937, 971, 992
Bsu36I CCTNAGG 1 cut(s) 586
BsuRI GGCC 3 cut(s) 496, 781, 1002
BtsCI GGATG 5 cut(s) 138, 511, 530, 990, 1171
BtsI GCAGTG 1 cut(s) 411
BtsIMutI CAGTG 1 cut(s) 411
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 1 cut(s) 1254
CaiI CAGNNNCTG 2 cut(s) 1022, 1181
CciI TCATGA 1 cut(s) 1219
Cfr13I GGNCC 5 cut(s) 494, 665, 780, 1038, 1280
Csp6I GTAC 3 cut(s) 393, 473, 652
CviAII CATG 6 cut(s) 151, 385, 410, 879, 980, 1220
CviQI GTAC 3 cut(s) 393, 473, 652
DdeI CTNAG 4 cut(s) 200, 263, 586, 594
DpnI GATC 7 cut(s) 103, 414, 568, 939, 973, 994, 1218
DpnII GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
DraI TTTAAA 2 cut(s) 211, 1293
Eam1104I CTCTTC 1 cut(s) 1021
EarI CTCTTC 1 cut(s) 1021
EciI GGCGGA 1 cut(s) 1059
Eco130I CCWWGG 1 cut(s) 128
Eco24I GRGCYC 1 cut(s) 15
Eco32I GATATC 1 cut(s) 906
Eco47I GGWCC 3 cut(s) 665, 1038, 1280
Eco57I CTGAAG 1 cut(s) 750
Eco81I CCTNAGG 1 cut(s) 586
Eco88I CYCGRG 1 cut(s) 661
EcoNI CCTNNNNNAGG 3 cut(s) 266, 729, 1026
EcoO109I RGGNCCY 1 cut(s) 1038
EcoRII CCWGG 2 cut(s) 940, 1021
EcoRV GATATC 1 cut(s) 906
EcoT14I CCWWGG 1 cut(s) 128
EcoT38I GRGCYC 1 cut(s) 15
ErhI CCWWGG 1 cut(s) 128
FaeI CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
FalI AAGNNNNNCTT 2 cut(s) 1267, 1299
FaqI GGGAC 5 cut(s) 104, 365, 557, 849, 1051
FatI CATG 6 cut(s) 150, 384, 409, 878, 979, 1219
FblI GTMKAC 1 cut(s) 852
Fnu4HI GCNGC 2 cut(s) 25, 324
FokI GGATG 5 cut(s) 145, 498, 517, 997, 1158
FriOI GRGCYC 1 cut(s) 15
Fsp4HI GCNGC 2 cut(s) 25, 324
FspBI CTAG 5 cut(s) 129, 308, 423, 774, 848
GluI GCNGC 2 cut(s) 25, 324
GsaI CCCAGC 3 cut(s) 495, 578, 584
GsuI CTGGAG 2 cut(s) 376, 894
HaeIII GGCC 3 cut(s) 496, 781, 1002
HapII CCGG 2 cut(s) 630, 1103
Hin1II CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
HincII GTYRAC 1 cut(s) 255
HindII GTYRAC 1 cut(s) 255
HindIII AAGCTT 1 cut(s) 1250
HinfI GANTC 3 cut(s) 746, 945, 1178
HpaI GTTAAC 1 cut(s) 255
HpaII CCGG 2 cut(s) 630, 1103
HphI GGTGA 6 cut(s) 152, 158, 719, 944, 1175, 1283
Hpy166II GTNNAC 2 cut(s) 255, 853
Hpy188I TCNGA 2 cut(s) 901, 1267
Hpy188III TCNNGA 4 cut(s) 588, 911, 1175, 1220
Hpy8I GTNNAC 2 cut(s) 255, 853
Hpy99I CGWCG 1 cut(s) 788
HpyAV CCTTC 5 cut(s) 239, 666, 669, 725, 1090
HpyCH4III ACNGT 4 cut(s) 191, 397, 433, 556
HpyCH4IV ACGT 1 cut(s) 711
HpyCH4V TGCA 4 cut(s) 323, 409, 1241, 1247
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 590, 1253
HpyF3I CTNAG 4 cut(s) 200, 263, 586, 594
HpySE526I ACGT 1 cut(s) 711
Hsp92II CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
KflI GGGWCCC 1 cut(s) 1038
KspAI GTTAAC 1 cut(s) 255
Kzo9I GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
LmnI GCTCC 2 cut(s) 292, 590
Lsp1109I GCAGC 2 cut(s) 36, 335
LweI GCATC 3 cut(s) 214, 850, 1228
MaeI CTAG 5 cut(s) 129, 308, 423, 774, 848
MaeII ACGT 1 cut(s) 711
MaeIII GTNAC 5 cut(s) 85, 523, 707, 1004, 1116
MalI GATC 7 cut(s) 103, 414, 568, 939, 973, 994, 1218
MboI GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
MboII GAAGA 6 cut(s) 111, 469, 537, 630, 947, 1038
MfeI CAATTG 2 cut(s) 702, 1242
MflI RGATCY 5 cut(s) 101, 566, 937, 971, 992
MhlI GDGCHC 2 cut(s) 15, 297
MluCI AATT 8 cut(s) 162, 621, 702, 862, 892, 1137, 1188, 1242
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 1 cut(s) 692
MseI TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
MslI CAYNNNNRTG 1 cut(s) 883
MspA1I CMGCKG 2 cut(s) 27, 1299
MspI CCGG 2 cut(s) 630, 1103
MspR9I CCNGG 3 cut(s) 630, 942, 1023
MunI CAATTG 2 cut(s) 702, 1242
MvaI CCWGG 2 cut(s) 942, 1023
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 212, 590, 1253
NciI CCSGG 1 cut(s) 630
NdeII GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
NlaIII CATG 6 cut(s) 154, 388, 413, 882, 983, 1223
NlaIV GGNNCC 4 cut(s) 666, 1039, 1040, 1101
NmuCI GTSAC 2 cut(s) 707, 1004
NspI RCATGY 1 cut(s) 388
PacI TTAATTAA 1 cut(s) 1140
PagI TCATGA 1 cut(s) 1219
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PfeI GAWTC 2 cut(s) 746, 945
PflMI CCANNNNNTGG 2 cut(s) 234, 1022
PfoI TCCNGGA 2 cut(s) 940, 1021
PkrI GCNGC 2 cut(s) 26, 325
PleI GAGTC 1 cut(s) 1172
PpsI GAGTC 1 cut(s) 1172
PpuMI RGGWCCY 1 cut(s) 1038
PshBI ATTAAT 1 cut(s) 861
PsiI TTATAA 1 cut(s) 812
Psp5II RGGWCCY 1 cut(s) 1038
Psp6I CCWGG 2 cut(s) 940, 1021
PspFI CCCAGC 3 cut(s) 491, 574, 580
PspGI CCWGG 2 cut(s) 940, 1021
PspN4I GGNNCC 4 cut(s) 666, 1039, 1040, 1101
PspPI GGNCC 5 cut(s) 494, 665, 780, 1038, 1280
PspPPI RGGWCCY 1 cut(s) 1038
PstNI CAGNNNCTG 2 cut(s) 1022, 1181
PsuI RGATCY 5 cut(s) 101, 566, 937, 971, 992
PvuII CAGCTG 1 cut(s) 1299
RsaI GTAC 3 cut(s) 394, 474, 653
RsaNI GTAC 3 cut(s) 393, 473, 652
RseI CAYNNNNRTG 1 cut(s) 883
SaqAI TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
SatI GCNGC 2 cut(s) 25, 324
Sau3AI GATC 7 cut(s) 101, 412, 566, 937, 971, 992, 1216
Sau96I GGNCC 5 cut(s) 494, 665, 780, 1038, 1280
ScaI AGTACT 1 cut(s) 394
SchI GAGTC 1 cut(s) 1172
ScrFI CCNGG 3 cut(s) 630, 942, 1023
SduI GDGCHC 2 cut(s) 15, 297
SfaNI GCATC 3 cut(s) 214, 850, 1228
SfcI CTRYAG 1 cut(s) 996
SinI GGWCC 3 cut(s) 665, 1038, 1280
SmiMI CAYNNNNRTG 1 cut(s) 883
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
Sse9I AATT 8 cut(s) 162, 621, 702, 862, 892, 1137, 1188, 1242
SsiI CCGC 3 cut(s) 27, 1070, 1278
SspMI CTAG 5 cut(s) 129, 308, 423, 774, 848
StyD4I CCNGG 3 cut(s) 628, 940, 1021
StyI CCWWGG 1 cut(s) 128
TaaI ACNGT 4 cut(s) 191, 397, 433, 556
TaiI ACGT 1 cut(s) 714
TasI AATT 8 cut(s) 162, 621, 702, 862, 892, 1137, 1188, 1242
TatI WGTACW 2 cut(s) 392, 472
TfiI GAWTC 2 cut(s) 746, 945
Tru1I TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
Tru9I TTAA 8 cut(s) 210, 254, 318, 861, 891, 1136, 1140, 1292
TscAI CASTG 1 cut(s) 411
TseFI GTSAC 2 cut(s) 707, 1004
TseI GCWGC 2 cut(s) 24, 323
Tsp45I GTSAC 2 cut(s) 707, 1004
TspDTI ATGAA 8 cut(s) 500, 537, 659, 735, 816, 888, 1001, 1236
TspRI CASTG 1 cut(s) 411
Van91I CCANNNNNTGG 2 cut(s) 234, 1022
VpaK11BI GGWCC 3 cut(s) 665, 1038, 1280
VspI ATTAAT 1 cut(s) 861
XagI CCTNNNNNAGG 3 cut(s) 266, 729, 1026
XapI RAATTY 2 cut(s) 162, 621
XceI RCATGY 1 cut(s) 388
XmaJI CCTAGG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 852
XspI CTAG 5 cut(s) 129, 308, 423, 774, 848
ZrmI AGTACT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.