Prupe.2G004600_v2.0.a1

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0009852
Physical Location & Seq
Reverse (-)
462313 .. 468272
5960 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G004600.1

Sequence Viewer

Length: 1341 bp
ATGGACTACGAGCCCTACGATAGCAGCGGAACTGATGACGATCTTCCTCCATCACACCAAAATAGAATTCCCAGAGGGGGTCGTGTTGCAGGGAATGGAAGATCTGCTGTGGGTTCCGTCCCATATCCTAGGATGTATGGTGATACTGACATGGAAGCACAAATTCACCAGCTTGAGCAAGAAGCATACAGTTCAGTTCTAAGAGCCTTTAAAGCTCAAGCTGATCTCATTACTTGGGAAAAGGAAAGTCTGATAACAGAACTTAGAAAGGAGTTGAGGCTATCGAACGAGGAGCATAGAGAACTTCTAGGACGGGTTAATGCAGATGATGTCATACGGAGGATAAGGGAGTGGAGACAGGCTGGTGGGGCTCAACTCGGCATGCACAATACTGTGCAAGCAGCTCATGATCCAATACCCAGTCCTACTGTCTCTGCATCTCGCAAGAAACAGAAGATGACCCAGTCAGTACATTCACAATCCTTTGTTGGGCCATCGCCACCATTTCATCCACAGGCTACCGCATCCCACCAGCCATCTTCATCTACTCTTAAACGAGGGTCTGTCCCAGCCCCAGCCCCAGCCCCAGCCCCAGCCCCGGGAGCCAAGGGAAAGAAACATAAACCTGGTCAAATTTTACCGGGTGCTTCTTCAATGAAGCAGTTCCCTTCCACAGGTCCGACAGGAAGAGGTCAAGTTTCGAATAGAGTCTCTTCCGGTGCCATTGTCAATGAACCTGCTGAAGGAACGACATATGATCCCTTGATTGGCAGGAAAGTAAGGACTAGGTGGCCTGATGACAACAACTTTTATGAAGCTGTTATAACGGACTACAATCAAGCTGAGGGACGACATGCCCTAGTCTACGATATTAATTCTGCAAATGAAACTTGGGAGTGGGTTAATCTATCAGAGATATCTCCTGAGGATATTCAATGGGTAGGTGAAGATCCTGGAATATCTCGTCGTGGGGGTTATGGTGGATCTGGTCATGGTATTAACAGATCTGTAGGCCGTGACAATGTTCCAGTTCCTGGAAGAGGTAGAGGGATCCCAAAGGGTCAATCAAGAAAAGATTTTCCACCGTCACAGAATGGCATTGGAAAGAAGGCTCCAGATGATATACAGTTACTTCACACTGATACTTTAATTAAGGAGGTAGAGAGAGTCTTTGGTGCAAATCATCCAGACCCTGTTGAAATTGAGAAAGCGAAGAAAGTGTTGAAAGACCATGAACAAGCACTTATCGATGCAATTGCAAAGCTTGCTGATATTTCTGACGGTGAAAGCGCTGACGGTGCCCAGCAGTTCTTACATAGGCAACCAATGGATCGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

447

Amino Acids

48.73

Weight (kDa)

6.58

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 824
Acc36I ACCTGC 1 cut(s) 745
AccB1I GGYRCC 2 cut(s) 719, 1298
AccB7I CCANNNNNTGG 1 cut(s) 1034
AccI GTMKAC 1 cut(s) 864
AciI CCGC 2 cut(s) 27, 522
AclWI GGATC 7 cut(s) 404, 752, 944, 991, 1045, 1058, 1338
AcsI RAATTY 3 cut(s) 66, 162, 633
AcuI CTGAAG 1 cut(s) 762
AfaI GTAC 1 cut(s) 471
AfeI AGCGCT 1 cut(s) 1291
AfiI CCNNNNNNNGG 9 cut(s) 77, 489, 598, 599, 674, 743, 767, 1034, 1040
AgsI TTSAA 4 cut(s) 654, 935, 1199, 1225
AjnI CCWGG 3 cut(s) 625, 952, 1033
AjuI GAANNNNNNNTTGG 2 cut(s) 51, 83
AluBI AGCT 7 cut(s) 172, 215, 221, 404, 818, 842, 1264
AluI AGCT 7 cut(s) 172, 215, 221, 404, 818, 842, 1264
Alw26I GTCTC 3 cut(s) 349, 436, 715
AlwI GGATC 7 cut(s) 404, 752, 944, 991, 1045, 1058, 1338
AlwNI CAGNNNCTG 2 cut(s) 1034, 1193
Ama87I CYCGRG 1 cut(s) 598
Aor51HI AGCGCT 1 cut(s) 1291
AoxI GGCC 3 cut(s) 491, 791, 1012
ApeKI GCWGC 2 cut(s) 24, 401
ApoI RAATTY 3 cut(s) 66, 162, 633
AseI ATTAAT 1 cut(s) 873
Asp700I GAANNNNTTC 1 cut(s) 662
AspA2I CCTAGG 1 cut(s) 128
AspLEI GCGC 1 cut(s) 1292
AspS9I GGNCC 2 cut(s) 491, 677
AsuC2I CCSGG 3 cut(s) 599, 600, 642
AsuHPI GGTGA 4 cut(s) 152, 158, 956, 1295
AsuII TTCGAA 1 cut(s) 701
AvaI CYCGRG 1 cut(s) 598
AvaII GGWCC 1 cut(s) 677
AvrII CCTAGG 1 cut(s) 128
AxyI CCTNAGG 1 cut(s) 924
BaeGI GKGCMC 1 cut(s) 1303
BamHI GGATCC 1 cut(s) 1050
BanI GGYRCC 2 cut(s) 719, 1298
BanII GRGCYC 2 cut(s) 15, 373
BbvCI CCTCAGC 1 cut(s) 843
BbvI GCAGC 2 cut(s) 36, 413
BccI CCATC 3 cut(s) 58, 502, 544
BceAI ACGGC 1 cut(s) 999
BcgI CGANNNNNNTGC 2 cut(s) 1238, 1272
BciT130I CCWGG 3 cut(s) 627, 954, 1035
BcnI CCSGG 3 cut(s) 599, 600, 642
BcoDI GTCTC 3 cut(s) 349, 436, 715
BfaI CTAG 4 cut(s) 129, 308, 786, 860
BfmI CTRYAG 1 cut(s) 1008
BfoI RGCGCY 1 cut(s) 1293
BfuAI ACCTGC 1 cut(s) 745
BglII AGATCT 2 cut(s) 101, 1004
BisI GCNGC 2 cut(s) 25, 402
BlnI CCTAGG 1 cut(s) 128
BlsI GCNGC 2 cut(s) 26, 403
Bme1390I CCNGG 6 cut(s) 599, 600, 627, 642, 954, 1035
Bme18I GGWCC 1 cut(s) 677
BmeT110I CYCGRG 1 cut(s) 598
BmgT120I GGNCC 2 cut(s) 491, 677
BmiI GGNNCC 6 cut(s) 115, 604, 721, 1052, 1113, 1300
BmrFI CCNGG 6 cut(s) 599, 600, 627, 642, 954, 1035
BmrI ACTGGG 2 cut(s) 414, 457
BmsI GCATC 3 cut(s) 446, 533, 1240
BmuI ACTGGG 2 cut(s) 414, 457
BpmI CTGGAG 1 cut(s) 1098
Bpu10I CCTNAGC 1 cut(s) 843
Bpu14I TTCGAA 1 cut(s) 701
BpuEI CTTGAG 2 cut(s) 194, 201
BpuMI CCSGG 3 cut(s) 599, 600, 642
Bsa29I ATCGAT 1 cut(s) 1248
BsaBI GATNNNNATC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 128, 597, 598, 606
BsaWI WCCGGW 1 cut(s) 716
Bsc4I CCNNNNNNNGG 9 cut(s) 77, 489, 598, 599, 674, 743, 767, 1034, 1040
Bse1I ACTGG 3 cut(s) 420, 463, 1028
Bse21I CCTNAGG 1 cut(s) 924
Bse8I GATNNNNATC 1 cut(s) 39
BseBI CCWGG 3 cut(s) 627, 954, 1035
BseCI ATCGAT 1 cut(s) 1248
BseDI CCNNGG 4 cut(s) 128, 597, 598, 606
BseGI GGATG 4 cut(s) 138, 508, 524, 1183
BseJI GATNNNNATC 1 cut(s) 39
BseLI CCNNNNNNNGG 9 cut(s) 77, 489, 598, 599, 674, 743, 767, 1034, 1040
BseMII CTCAG 2 cut(s) 834, 915
BseNI ACTGG 3 cut(s) 420, 463, 1028
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 1303
BseXI GCAGC 2 cut(s) 36, 413
BseYI CCCAGC 6 cut(s) 568, 574, 580, 586, 592, 1302
BshFI GGCC 3 cut(s) 493, 793, 1014
BshNI GGYRCC 2 cut(s) 719, 1298
BshVI ATCGAT 1 cut(s) 1248
BsiHKCI CYCGRG 1 cut(s) 598
BsiSI CCGG 3 cut(s) 599, 641, 717
BslFI GGGAC 3 cut(s) 104, 551, 861
BslI CCNNNNNNNGG 9 cut(s) 77, 489, 598, 599, 674, 743, 767, 1034, 1040
BsmAI GTCTC 3 cut(s) 349, 436, 715
BsmFI GGGAC 3 cut(s) 104, 551, 861
BsnI GGCC 3 cut(s) 493, 793, 1014
BsoBI CYCGRG 1 cut(s) 598
Bsp119I TTCGAA 1 cut(s) 701
Bsp1286I GDGCHC 3 cut(s) 15, 373, 1303
BspACI CCGC 2 cut(s) 27, 522
BspANI GGCC 3 cut(s) 493, 793, 1014
BspCNI CTCAG 2 cut(s) 835, 916
BspDI ATCGAT 1 cut(s) 1248
BspHI TCATGA 1 cut(s) 406
BspLI GGNNCC 6 cut(s) 115, 604, 721, 1052, 1113, 1300
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 7 cut(s) 404, 752, 944, 991, 1045, 1058, 1338
BspT104I TTCGAA 1 cut(s) 701
BspT107I GGYRCC 2 cut(s) 719, 1298
BsrI ACTGG 3 cut(s) 420, 463, 1028
BssECI CCNNGG 4 cut(s) 128, 597, 598, 606
BssT1I CCWWGG 2 cut(s) 128, 606
Bst2UI CCWGG 3 cut(s) 627, 954, 1035
Bst4CI ACNGT 7 cut(s) 191, 394, 430, 1086, 1128, 1283, 1298
Bst6I CTCTTC 3 cut(s) 682, 718, 1033
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstBI TTCGAA 1 cut(s) 701
BstC8I GCNNGC 3 cut(s) 383, 399, 1266
BstDEI CTNAG 4 cut(s) 200, 263, 843, 924
BstENI CCTNNNNNAGG 3 cut(s) 672, 741, 1038
BstF5I GGATG 4 cut(s) 138, 508, 524, 1183
BstH2I RGCGCY 1 cut(s) 1293
BstHHI GCGC 1 cut(s) 1292
BstMAI GTCTC 3 cut(s) 349, 436, 715
BstMWI GCNNNNNNNGC 5 cut(s) 212, 368, 602, 1265, 1298
BstNI CCWGG 3 cut(s) 627, 954, 1035
BstNSI RCATGY 2 cut(s) 385, 857
BstSCI CCNGG 6 cut(s) 597, 598, 625, 640, 952, 1033
BstSFI CTRYAG 1 cut(s) 1008
BstSLI GKGCMC 1 cut(s) 1303
BstV1I GCAGC 2 cut(s) 36, 413
BstX2I RGATCY 5 cut(s) 101, 949, 983, 1004, 1050
BstYI RGATCY 5 cut(s) 101, 949, 983, 1004, 1050
Bsu15I ATCGAT 1 cut(s) 1248
Bsu36I CCTNAGG 1 cut(s) 924
BsuRI GGCC 3 cut(s) 493, 793, 1014
BsuTUI ATCGAT 1 cut(s) 1248
BtgZI GCGATG 1 cut(s) 480
BtsCI GGATG 4 cut(s) 138, 508, 524, 1183
BtsIMutI CAGTG 1 cut(s) 1137
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 3 cut(s) 383, 399, 1266
CaiI CAGNNNCTG 2 cut(s) 1034, 1193
CciI TCATGA 1 cut(s) 406
CfoI GCGC 1 cut(s) 1292
Cfr13I GGNCC 2 cut(s) 491, 677
Cfr9I CCCGGG 1 cut(s) 598
ClaI ATCGAT 1 cut(s) 1248
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 1 cut(s) 470
CviAII CATG 6 cut(s) 151, 382, 407, 854, 992, 1232
CviQI GTAC 1 cut(s) 470
DdeI CTNAG 4 cut(s) 200, 263, 843, 924
DraI TTTAAA 1 cut(s) 211
Eam1104I CTCTTC 3 cut(s) 682, 718, 1033
EarI CTCTTC 3 cut(s) 682, 718, 1033
Eco130I CCWWGG 2 cut(s) 128, 606
Eco24I GRGCYC 2 cut(s) 15, 373
Eco32I GATATC 1 cut(s) 918
Eco47I GGWCC 1 cut(s) 677
Eco47III AGCGCT 1 cut(s) 1291
Eco57I CTGAAG 1 cut(s) 762
Eco81I CCTNAGG 1 cut(s) 924
Eco88I CYCGRG 1 cut(s) 598
EcoNI CCTNNNNNAGG 3 cut(s) 672, 741, 1038
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 3 cut(s) 625, 952, 1033
EcoRV GATATC 1 cut(s) 918
EcoT14I CCWWGG 2 cut(s) 128, 606
EcoT38I GRGCYC 2 cut(s) 15, 373
ErhI CCWWGG 2 cut(s) 128, 606
FaeI CATG 6 cut(s) 154, 385, 410, 857, 995, 1235
FaqI GGGAC 3 cut(s) 104, 551, 861
FatI CATG 6 cut(s) 150, 381, 406, 853, 991, 1231
FauNDI CATATG 1 cut(s) 754
FblI GTMKAC 1 cut(s) 864
Fnu4HI GCNGC 2 cut(s) 25, 402
FokI GGATG 4 cut(s) 145, 495, 511, 1170
FriOI GRGCYC 2 cut(s) 15, 373
Fsp4HI GCNGC 2 cut(s) 25, 402
FspBI CTAG 4 cut(s) 129, 308, 786, 860
GlaI GCGC 1 cut(s) 1291
GluI GCNGC 2 cut(s) 25, 402
GsaI CCCAGC 6 cut(s) 572, 578, 584, 590, 596, 1306
GsuI CTGGAG 1 cut(s) 1098
HaeII RGCGCY 1 cut(s) 1293
HaeIII GGCC 3 cut(s) 493, 793, 1014
HapII CCGG 3 cut(s) 599, 641, 717
HhaI GCGC 1 cut(s) 1292
Hin1II CATG 6 cut(s) 154, 385, 410, 857, 995, 1235
Hin6I GCGC 1 cut(s) 1290
HinP1I GCGC 1 cut(s) 1290
HindIII AAGCTT 1 cut(s) 1262
HinfI GANTC 2 cut(s) 708, 1167
HpaII CCGG 3 cut(s) 599, 641, 717
HphI GGTGA 4 cut(s) 152, 158, 956, 1295
Hpy166II GTNNAC 1 cut(s) 865
Hpy188I TCNGA 4 cut(s) 252, 681, 913, 1279
Hpy188III TCNNGA 6 cut(s) 407, 923, 1068, 1115, 1187, 1334
Hpy8I GTNNAC 1 cut(s) 865
Hpy99I CGWCG 1 cut(s) 969
HpyAV CCTTC 3 cut(s) 678, 737, 1102
HpyCH4III ACNGT 7 cut(s) 191, 394, 430, 1086, 1128, 1283, 1298
HpyCH4V TGCA 9 cut(s) 89, 323, 385, 397, 437, 881, 1178, 1253, 1259
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 368, 602, 1265, 1298
HpyF3I CTNAG 4 cut(s) 200, 263, 843, 924
Hsp92II CATG 6 cut(s) 154, 385, 410, 857, 995, 1235
HspAI GCGC 1 cut(s) 1290
LmnI GCTCC 3 cut(s) 292, 602, 1117
Lsp1109I GCAGC 2 cut(s) 36, 413
LweI GCATC 3 cut(s) 446, 533, 1240
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 4 cut(s) 129, 308, 786, 860
MaeIII GTNAC 3 cut(s) 1016, 1086, 1128
MfeI CAATTG 1 cut(s) 1254
MflI RGATCY 5 cut(s) 101, 949, 983, 1004, 1050
MhlI GDGCHC 3 cut(s) 15, 373, 1303
MluCI AATT 7 cut(s) 66, 162, 633, 874, 1149, 1200, 1254
MlyI GAGTC 2 cut(s) 717, 1176
MmeI TCCRAC 1 cut(s) 704
MroXI GAANNNNTTC 1 cut(s) 662
MseI TTAA 8 cut(s) 210, 318, 552, 873, 903, 999, 1148, 1152
MspA1I CMGCKG 1 cut(s) 27
MspI CCGG 3 cut(s) 599, 641, 717
MspR9I CCNGG 6 cut(s) 599, 600, 627, 642, 954, 1035
MunI CAATTG 1 cut(s) 1254
MvaI CCWGG 3 cut(s) 627, 954, 1035
MwoI GCNNNNNNNGC 5 cut(s) 212, 368, 602, 1265, 1298
NciI CCSGG 3 cut(s) 599, 600, 642
NdeI CATATG 1 cut(s) 754
NlaIII CATG 6 cut(s) 154, 385, 410, 857, 995, 1235
NlaIV GGNNCC 6 cut(s) 115, 604, 721, 1052, 1113, 1300
NmeAIII GCCGAG 1 cut(s) 357
NmuCI GTSAC 2 cut(s) 1016, 1086
NspI RCATGY 2 cut(s) 385, 857
NspV TTCGAA 1 cut(s) 701
PacI TTAATTAA 1 cut(s) 1152
PaeI GCATGC 1 cut(s) 385
PagI TCATGA 1 cut(s) 406
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 662
PflFI GACNNNGTC 1 cut(s) 463
PflMI CCANNNNNTGG 1 cut(s) 1034
PfoI TCCNGGA 2 cut(s) 952, 1033
PkrI GCNGC 2 cut(s) 26, 403
PleI GAGTC 2 cut(s) 716, 1175
PpsI GAGTC 2 cut(s) 716, 1175
PshBI ATTAAT 1 cut(s) 873
PsiI TTATAA 1 cut(s) 824
Psp6I CCWGG 3 cut(s) 625, 952, 1033
PspFI CCCAGC 6 cut(s) 568, 574, 580, 586, 592, 1302
PspGI CCWGG 3 cut(s) 625, 952, 1033
PspN4I GGNNCC 6 cut(s) 115, 604, 721, 1052, 1113, 1300
PspPI GGNCC 2 cut(s) 491, 677
PstNI CAGNNNCTG 2 cut(s) 1034, 1193
PsuI RGATCY 5 cut(s) 101, 949, 983, 1004, 1050
PsyI GACNNNGTC 1 cut(s) 463
RsaI GTAC 1 cut(s) 471
RsaNI GTAC 1 cut(s) 470
SaqAI TTAA 8 cut(s) 210, 318, 552, 873, 903, 999, 1148, 1152
SatI GCNGC 2 cut(s) 25, 402
Sau96I GGNCC 2 cut(s) 491, 677
SchI GAGTC 2 cut(s) 717, 1176
ScrFI CCNGG 6 cut(s) 599, 600, 627, 642, 954, 1035
SduI GDGCHC 3 cut(s) 15, 373, 1303
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 3 cut(s) 446, 533, 1240
SfcI CTRYAG 1 cut(s) 1008
SfuI TTCGAA 1 cut(s) 701
SinI GGWCC 1 cut(s) 677
SmaI CCCGGG 1 cut(s) 600
SmlI CTYRAG 2 cut(s) 173, 216
SmoI CTYRAG 2 cut(s) 173, 216
SphI GCATGC 1 cut(s) 385
Sse9I AATT 7 cut(s) 66, 162, 633, 874, 1149, 1200, 1254
SsiI CCGC 2 cut(s) 27, 522
SspMI CTAG 4 cut(s) 129, 308, 786, 860
StyD4I CCNGG 6 cut(s) 597, 598, 625, 640, 952, 1033
StyI CCWWGG 2 cut(s) 128, 606
TaaI ACNGT 7 cut(s) 191, 394, 430, 1086, 1128, 1283, 1298
TaqI TCGA 4 cut(s) 284, 701, 1248, 1333
TasI AATT 7 cut(s) 66, 162, 633, 874, 1149, 1200, 1254
TatI WGTACW 1 cut(s) 469
Tru1I TTAA 8 cut(s) 210, 318, 552, 873, 903, 999, 1148, 1152
Tru9I TTAA 8 cut(s) 210, 318, 552, 873, 903, 999, 1148, 1152
TscAI CASTG 1 cut(s) 1144
TseFI GTSAC 2 cut(s) 1016, 1086
TseI GCWGC 2 cut(s) 24, 401
Tsp45I GTSAC 2 cut(s) 1016, 1086
TspDTI ATGAA 7 cut(s) 497, 531, 671, 747, 828, 900, 1248
TspGWI ACGGA 3 cut(s) 106, 352, 842
TspMI CCCGGG 1 cut(s) 598
TspRI CASTG 1 cut(s) 1144
Tth111I GACNNNGTC 1 cut(s) 463
Van91I CCANNNNNTGG 1 cut(s) 1034
VpaK11BI GGWCC 1 cut(s) 677
VspI ATTAAT 1 cut(s) 873
XagI CCTNNNNNAGG 3 cut(s) 672, 741, 1038
XapI RAATTY 3 cut(s) 66, 162, 633
XceI RCATGY 2 cut(s) 385, 857
XmaI CCCGGG 1 cut(s) 598
XmaJI CCTAGG 1 cut(s) 128
XmiI GTMKAC 1 cut(s) 864
XmnI GAANNNNTTC 1 cut(s) 662
XspI CTAG 4 cut(s) 129, 308, 786, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.