Rw1G000240

ENT

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009852
Physical Location & Seq
Reverse (-)
313806 .. 318765
4960 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G000240.1

Sequence Viewer

Length: 1311 bp
ATGGAGCACGAGCCATACGATAGCAGCGGAACTGATGACGATCTTCCTCCATCACATCAAAATAGAATTTCTAGAGGGGGTCGTGTTGCAGGGAATGGAAGATCTGCTGTGGGTTCTGCCCCATATTCTAGGATGTATGGTGAAACTGGCATGGAAGCACAAATTCATCAGCTTGAAAAAGAAGCATACAGTTCAGTTCTAAGAGCTTTTAAAGCTCAAGCTGATCAAATTACTTGGGAAAAGGAAAGTGTGATAACAGAACTTAGAAAGGAGTTGAGGTTATCGAATGAGGAGCACAGAGAACTTCTGGGACGGGTTAATGCAGATGATGTCCTGCAGAGGATAAGGGAGTGGAGACAGGGAGGTGGGATTCAAACAGGCATGCTCAGTACTGTTCAAGCTGTCCATGATCCGTTGCCCAGTCCTACTGTTTCTGCATCTCGGAAGAAACAGAAGACTACACAATCAATACATTCGCAATCCTTTGTTGGGCCATCACCACCATTTCTTCCACAGCCATCAACCACATCCCACCAGCCATCTTCATCCAATGTGAAGAGAGGATCTGTCCCAGTCCCAGCCCCAGTAGCCAAGGGCAAGAAACATAAATCTGGTCAAATTTTACCTGGTGCTTCTTCAATAAAGCCGCACCCTTCTTCAGGAAGGGGTCAAGTTTCTAATAGAGTCTCATCTGGTGCAATTAGGAATGAGCCTGTGGAAGGAGCGACACTTAATCCTTTCATTGGGAAAAGAGTTAGGACTAGGTGGCCGGAGGACAACAGCTTTTATGAAGCTGTTATATCGGACTATGATGCAGCTAAGGCATTACATGTACTAGTCTACGATATTAATTCTGCTGACGAATCGTGGGAATGGGTTAATCTATCAGAGATATCTCCTGAGGATATTCAATGGGTAGATGAAGATCCTGGTATCTCTCAACGTGGGGGTTATGGTGGATCAGGTCATGGGATGAATAGATCTGTAGGCCGTGATAGTGTTCCAGTTTCTGGAAGAGGTAGAGGAATCCGAAAGGGTCAATCAAGAAAAGATTTTCTGCCATCACAGAATGGCGTTGGAAAGAAGGCGCCAGATGATATACAGTTACTTCACACAGATACTTTAATTAAGGAGGTAGAGAGAGTCTTTGGTGCAAATCATCCAGACCCTGTTGAAATTGATAAAGCAAAGAAAGTTTTGAAAGATCATGAGCAAGCACTTATTGATGCAATTGCAAAGCTCACTGATATTTCTGACGGTGAAAGCGCTGAGGGTGGCCACCAGTTCTTACGTGGGCAACCAATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ENT LBR_tudor

Protein Analysis

436

Amino Acids

47.59

Weight (kDa)

6.75

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENT PF03735 53 - 121 1.2e-29 ENT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1087
AccB7I CCANNNNNTGG 1 cut(s) 1010
AccI GTMKAC 1 cut(s) 840
AciI CCGC 2 cut(s) 27, 647
AclWI GGATC 4 cut(s) 404, 571, 920, 967
AcoI YGGCCR 2 cut(s) 767, 1276
AcsI RAATTY 3 cut(s) 66, 162, 618
AcuI CTGAAG 1 cut(s) 642
AcyI GRCGYC 1 cut(s) 1088
AfaI GTAC 2 cut(s) 391, 834
AfeI AGCGCT 1 cut(s) 1267
AfiI CCNNNNNNNGG 5 cut(s) 489, 659, 719, 743, 1010
AflIII ACRYGT 1 cut(s) 829
AgsI TTSAA 7 cut(s) 176, 374, 398, 639, 911, 1175, 1201
AhlI ACTAGT 1 cut(s) 835
AjnI CCWGG 2 cut(s) 625, 928
AluBI AGCT 9 cut(s) 172, 206, 215, 221, 401, 783, 794, 818, 1240
AluI AGCT 9 cut(s) 172, 206, 215, 221, 401, 783, 794, 818, 1240
Alw21I GWGCWC 2 cut(s) 9, 297
Alw26I GTCTC 2 cut(s) 349, 691
AlwI GGATC 4 cut(s) 404, 571, 920, 967
AlwNI CAGNNNCTG 2 cut(s) 1010, 1169
Aor51HI AGCGCT 1 cut(s) 1267
AoxI GGCC 4 cut(s) 491, 767, 988, 1276
ApeKI GCWGC 2 cut(s) 24, 815
ApoI RAATTY 3 cut(s) 66, 162, 618
AseI ATTAAT 1 cut(s) 849
AspLEI GCGC 2 cut(s) 1090, 1268
AspS9I GGNCC 1 cut(s) 491
AsuHPI GGTGA 3 cut(s) 152, 489, 1271
AxyI CCTNAGG 1 cut(s) 900
BalI TGGCCA 1 cut(s) 1278
BanI GGYRCC 1 cut(s) 1087
BauI CACGAG 1 cut(s) 8
BbsI GAAGAC 1 cut(s) 461
Bbv12I GWGCWC 2 cut(s) 9, 297
BbvCI CCTCAGC 1 cut(s) 1269
BbvI GCAGC 2 cut(s) 36, 827
BccI CCATC 5 cut(s) 58, 502, 526, 547, 1069
BceAI ACGGC 1 cut(s) 975
BciT130I CCWGG 2 cut(s) 627, 930
BclI TGATCA 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 349, 691
BcuI ACTAGT 1 cut(s) 835
BfaI CTAG 4 cut(s) 72, 129, 762, 836
BfmI CTRYAG 2 cut(s) 335, 984
BfoI RGCGCY 2 cut(s) 1091, 1269
BglII AGATCT 2 cut(s) 101, 980
BisI GCNGC 3 cut(s) 25, 647, 816
BlsI GCNGC 3 cut(s) 26, 648, 817
BmcAI AGTACT 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 627, 930
BmgT120I GGNCC 1 cut(s) 491
BmiI GGNNCC 1 cut(s) 1089
BmrFI CCNGG 2 cut(s) 627, 930
BmrI ACTGGG 3 cut(s) 414, 566, 578
BmsI GCATC 3 cut(s) 446, 802, 1216
BmuI ACTGGG 3 cut(s) 414, 566, 578
BpiI GAAGAC 1 cut(s) 461
Bpu10I CCTNAGC 2 cut(s) 819, 1269
BpuEI CTTGAG 1 cut(s) 201
BsaAI YACGTR 1 cut(s) 1292
BsaBI GATNNNNATC 2 cut(s) 39, 924
BsaHI GRCGYC 1 cut(s) 1088
BsaJI CCNNGG 1 cut(s) 591
BsaXI ACNNNNNCTCC 2 cut(s) 263, 293
Bsc4I CCNNNNNNNGG 5 cut(s) 489, 659, 719, 743, 1010
Bse1I ACTGG 6 cut(s) 151, 420, 572, 584, 1004, 1282
Bse21I CCTNAGG 1 cut(s) 900
Bse8I GATNNNNATC 2 cut(s) 39, 924
BseBI CCWGG 2 cut(s) 627, 930
BseDI CCNNGG 1 cut(s) 591
BseGI GGATG 5 cut(s) 138, 527, 545, 978, 1159
BseJI GATNNNNATC 2 cut(s) 39, 924
BseLI CCNNNNNNNGG 5 cut(s) 489, 659, 719, 743, 1010
BseMII CTCAG 3 cut(s) 400, 891, 1260
BseNI ACTGG 6 cut(s) 151, 420, 572, 584, 1004, 1282
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 2 cut(s) 36, 827
BseYI CCCAGC 1 cut(s) 577
BshFI GGCC 4 cut(s) 493, 769, 990, 1278
BshNI GGYRCC 1 cut(s) 1087
BsiHKAI GWGCWC 2 cut(s) 9, 297
BsiSI CCGG 1 cut(s) 770
BslFI GGGAC 3 cut(s) 324, 554, 560
BslI CCNNNNNNNGG 5 cut(s) 489, 659, 719, 743, 1010
BsmAI GTCTC 2 cut(s) 349, 691
BsmFI GGGAC 3 cut(s) 324, 554, 560
BsnI GGCC 4 cut(s) 493, 769, 990, 1278
Bsp1286I GDGCHC 2 cut(s) 9, 297
Bsp143I GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
BspACI CCGC 2 cut(s) 27, 647
BspANI GGCC 4 cut(s) 493, 769, 990, 1278
BspCNI CTCAG 3 cut(s) 399, 892, 1261
BspHI TCATGA 1 cut(s) 1207
BspLI GGNNCC 1 cut(s) 1089
BspMAI CTGCAG 1 cut(s) 339
BspPI GGATC 4 cut(s) 404, 571, 920, 967
BspT107I GGYRCC 1 cut(s) 1087
BsrI ACTGG 6 cut(s) 151, 420, 572, 584, 1004, 1282
BssECI CCNNGG 1 cut(s) 591
BssMI GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
BssNI GRCGYC 1 cut(s) 1088
BssSI CACGAG 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 591
Bst2BI CACGAG 1 cut(s) 8
Bst2UI CCWGG 2 cut(s) 627, 930
Bst4CI ACNGT 5 cut(s) 191, 394, 430, 1104, 1259
Bst6I CTCTTC 2 cut(s) 551, 1009
BstACI GRCGYC 1 cut(s) 1088
BstBAI YACGTR 1 cut(s) 1292
BstC8I GCNNGC 2 cut(s) 383, 1215
BstDEI CTNAG 6 cut(s) 200, 263, 386, 819, 900, 1269
BstENI CCTNNNNNAGG 2 cut(s) 657, 717
BstF5I GGATG 5 cut(s) 138, 527, 545, 978, 1159
BstH2I RGCGCY 2 cut(s) 1091, 1269
BstHHI GCGC 2 cut(s) 1090, 1268
BstKTI GATC 9 cut(s) 43, 104, 226, 412, 566, 928, 962, 983, 1207
BstMAI GTCTC 2 cut(s) 349, 691
BstMBI GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
BstMWI GCNNNNNNNGC 3 cut(s) 212, 587, 821
BstNI CCWGG 2 cut(s) 627, 930
BstNSI RCATGY 2 cut(s) 385, 833
BstSCI CCNGG 2 cut(s) 625, 928
BstSFI CTRYAG 2 cut(s) 335, 984
BstV1I GCAGC 2 cut(s) 36, 827
BstV2I GAAGAC 1 cut(s) 461
BstX2I RGATCY 4 cut(s) 101, 563, 925, 980
BstYI RGATCY 4 cut(s) 101, 563, 925, 980
Bsu36I CCTNAGG 1 cut(s) 900
BsuRI GGCC 4 cut(s) 493, 769, 990, 1278
BtsCI GGATG 5 cut(s) 138, 527, 545, 978, 1159
BtsIMutI CAGTG 1 cut(s) 1242
Cac8I GCNNGC 2 cut(s) 383, 1215
CaiI CAGNNNCTG 2 cut(s) 1010, 1169
CciI TCATGA 1 cut(s) 1207
CfoI GCGC 2 cut(s) 1090, 1268
Cfr13I GGNCC 1 cut(s) 491
CsiI ACCWGGT 1 cut(s) 625
Csp6I GTAC 2 cut(s) 390, 833
CviAII CATG 6 cut(s) 151, 382, 407, 830, 968, 1208
CviQI GTAC 2 cut(s) 390, 833
DdeI CTNAG 6 cut(s) 200, 263, 386, 819, 900, 1269
DinI GGCGCC 1 cut(s) 1089
DpnI GATC 9 cut(s) 42, 103, 225, 411, 565, 927, 961, 982, 1206
DpnII GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
DraI TTTAAA 1 cut(s) 211
EaeI YGGCCR 2 cut(s) 767, 1276
Eam1104I CTCTTC 2 cut(s) 551, 1009
EarI CTCTTC 2 cut(s) 551, 1009
Eco130I CCWWGG 1 cut(s) 591
Eco32I GATATC 1 cut(s) 894
Eco47III AGCGCT 1 cut(s) 1267
Eco57I CTGAAG 1 cut(s) 642
Eco81I CCTNAGG 1 cut(s) 900
EcoNI CCTNNNNNAGG 2 cut(s) 657, 717
EcoRII CCWGG 2 cut(s) 625, 928
EcoRV GATATC 1 cut(s) 894
EcoT14I CCWWGG 1 cut(s) 591
EgeI GGCGCC 1 cut(s) 1089
EheI GGCGCC 1 cut(s) 1089
ErhI CCWWGG 1 cut(s) 591
FaeI CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
FaqI GGGAC 3 cut(s) 324, 554, 560
FatI CATG 6 cut(s) 150, 381, 406, 829, 967, 1207
FbaI TGATCA 1 cut(s) 223
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 3 cut(s) 25, 647, 816
FokI GGATG 5 cut(s) 145, 514, 532, 985, 1146
Fsp4HI GCNGC 3 cut(s) 25, 647, 816
FspBI CTAG 4 cut(s) 72, 129, 762, 836
GlaI GCGC 2 cut(s) 1089, 1267
GluI GCNGC 3 cut(s) 25, 647, 816
GsaI CCCAGC 1 cut(s) 581
HaeII RGCGCY 2 cut(s) 1091, 1269
HaeIII GGCC 4 cut(s) 493, 769, 990, 1278
HapII CCGG 1 cut(s) 770
HhaI GCGC 2 cut(s) 1090, 1268
Hin1I GRCGYC 1 cut(s) 1088
Hin1II CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
Hin6I GCGC 2 cut(s) 1088, 1266
HinP1I GCGC 2 cut(s) 1088, 1266
HinfI GANTC 5 cut(s) 370, 684, 863, 1026, 1143
HpaII CCGG 1 cut(s) 770
HphI GGTGA 3 cut(s) 152, 489, 1271
Hpy166II GTNNAC 1 cut(s) 841
Hpy188I TCNGA 5 cut(s) 444, 805, 889, 1031, 1255
Hpy188III TCNNGA 7 cut(s) 72, 660, 899, 1011, 1044, 1163, 1208
Hpy8I GTNNAC 1 cut(s) 841
HpyAV CCTTC 4 cut(s) 657, 663, 713, 1078
HpyCH4III ACNGT 5 cut(s) 191, 394, 430, 1104, 1259
HpyCH4IV ACGT 2 cut(s) 943, 1291
HpyCH4V TGCA 9 cut(s) 89, 323, 337, 437, 698, 815, 1154, 1229, 1235
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 587, 821
HpyF3I CTNAG 6 cut(s) 200, 263, 386, 819, 900, 1269
HpySE526I ACGT 2 cut(s) 943, 1291
Hsp92I GRCGYC 1 cut(s) 1088
Hsp92II CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
HspAI GCGC 2 cut(s) 1088, 1266
KasI GGCGCC 1 cut(s) 1087
Ksp22I TGATCA 1 cut(s) 223
Kzo9I GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
LmnI GCTCC 3 cut(s) 4, 292, 722
Lsp1109I GCAGC 2 cut(s) 36, 827
LweI GCATC 3 cut(s) 446, 802, 1216
MabI ACCWGGT 1 cut(s) 625
MaeI CTAG 4 cut(s) 72, 129, 762, 836
MaeII ACGT 2 cut(s) 943, 1291
MaeIII GTNAC 1 cut(s) 1104
MalI GATC 9 cut(s) 42, 103, 225, 411, 565, 927, 961, 982, 1206
MboI GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
MfeI CAATTG 1 cut(s) 1230
MflI RGATCY 4 cut(s) 101, 563, 925, 980
MhlI GDGCHC 2 cut(s) 9, 297
MlsI TGGCCA 1 cut(s) 1278
MluCI AATT 9 cut(s) 66, 162, 228, 618, 699, 850, 1125, 1176, 1230
MluNI TGGCCA 1 cut(s) 1278
Mly113I GGCGCC 1 cut(s) 1088
MlyI GAGTC 2 cut(s) 693, 1152
MmeI TCCRAC 1 cut(s) 1057
Mox20I TGGCCA 1 cut(s) 1278
MscI TGGCCA 1 cut(s) 1278
MseI TTAA 7 cut(s) 210, 318, 732, 849, 879, 1124, 1128
Msp20I TGGCCA 1 cut(s) 1278
MspA1I CMGCKG 1 cut(s) 27
MspI CCGG 1 cut(s) 770
MspR9I CCNGG 2 cut(s) 627, 930
MunI CAATTG 1 cut(s) 1230
MvaI CCWGG 2 cut(s) 627, 930
MwoI GCNNNNNNNGC 3 cut(s) 212, 587, 821
NarI GGCGCC 1 cut(s) 1088
NdeII GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
NlaIII CATG 6 cut(s) 154, 385, 410, 833, 971, 1211
NlaIV GGNNCC 1 cut(s) 1089
NspI RCATGY 2 cut(s) 385, 833
PacI TTAATTAA 1 cut(s) 1128
PaeI GCATGC 1 cut(s) 385
PagI TCATGA 1 cut(s) 1207
PciI ACATGT 1 cut(s) 829
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PfeI GAWTC 3 cut(s) 370, 863, 1026
PflMI CCANNNNNTGG 1 cut(s) 1010
PkrI GCNGC 3 cut(s) 26, 648, 817
PleI GAGTC 2 cut(s) 692, 1151
PluTI GGCGCC 1 cut(s) 1091
PpsI GAGTC 2 cut(s) 692, 1151
Ppu21I YACGTR 1 cut(s) 1292
PscI ACATGT 1 cut(s) 829
PshBI ATTAAT 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 625, 928
PspFI CCCAGC 1 cut(s) 577
PspGI CCWGG 2 cut(s) 625, 928
PspN4I GGNNCC 1 cut(s) 1089
PspPI GGNCC 1 cut(s) 491
PstI CTGCAG 1 cut(s) 339
PstNI CAGNNNCTG 2 cut(s) 1010, 1169
PsuI RGATCY 4 cut(s) 101, 563, 925, 980
RsaI GTAC 2 cut(s) 391, 834
RsaNI GTAC 2 cut(s) 390, 833
SaqAI TTAA 7 cut(s) 210, 318, 732, 849, 879, 1124, 1128
SatI GCNGC 3 cut(s) 25, 647, 816
Sau3AI GATC 9 cut(s) 40, 101, 223, 409, 563, 925, 959, 980, 1204
Sau96I GGNCC 1 cut(s) 491
ScaI AGTACT 1 cut(s) 391
SchI GAGTC 2 cut(s) 693, 1152
ScrFI CCNGG 2 cut(s) 627, 930
SduI GDGCHC 2 cut(s) 9, 297
SexAI ACCWGGT 1 cut(s) 625
SfaNI GCATC 3 cut(s) 446, 802, 1216
SfcI CTRYAG 2 cut(s) 335, 984
SfoI GGCGCC 1 cut(s) 1089
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
SpeI ACTAGT 1 cut(s) 835
SphI GCATGC 1 cut(s) 385
Sse9I AATT 9 cut(s) 66, 162, 228, 618, 699, 850, 1125, 1176, 1230
SsiI CCGC 2 cut(s) 27, 647
SspDI GGCGCC 1 cut(s) 1087
SspMI CTAG 4 cut(s) 72, 129, 762, 836
StyD4I CCNGG 2 cut(s) 625, 928
StyI CCWWGG 1 cut(s) 591
TaaI ACNGT 5 cut(s) 191, 394, 430, 1104, 1259
TaiI ACGT 2 cut(s) 946, 1294
TaqI TCGA 1 cut(s) 284
TasI AATT 9 cut(s) 66, 162, 228, 618, 699, 850, 1125, 1176, 1230
TatI WGTACW 2 cut(s) 389, 832
TauI GCSGC 1 cut(s) 649
TfiI GAWTC 3 cut(s) 370, 863, 1026
Tru1I TTAA 7 cut(s) 210, 318, 732, 849, 879, 1124, 1128
Tru9I TTAA 7 cut(s) 210, 318, 732, 849, 879, 1124, 1128
TscAI CASTG 1 cut(s) 1249
TseI GCWGC 2 cut(s) 24, 815
TspDTI ATGAA 6 cut(s) 155, 534, 730, 804, 936, 989
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 1 cut(s) 1249
Van91I CCANNNNNTGG 1 cut(s) 1010
VspI ATTAAT 1 cut(s) 849
XagI CCTNNNNNAGG 2 cut(s) 657, 717
XapI RAATTY 3 cut(s) 66, 162, 618
XbaI TCTAGA 1 cut(s) 71
XceI RCATGY 2 cut(s) 385, 833
XcmI CCANNNNNNNNNTGG 1 cut(s) 1289
XmiI GTMKAC 1 cut(s) 840
XspI CTAG 4 cut(s) 72, 129, 762, 836
ZrmI AGTACT 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.